Structural characterization and distribution of zebrafish germ plasm during interphase and mitosis

In zebrafish, germ cells are responsible for transmitting the genetic information from one generation to the next. During the first cleavages of zebrafish embryonic development, a specialized part of the cytoplasm known as germ plasm, is responsible of committing four blastomeres to become the progenitors of all germ cells in the forming embryo. Much is known about how the germ plasm is spatially distributed in early stages of primordial germ cell development, a process described to be dependant on microtubules and actin. However, little is known about how the material is inherited after it reorganizes into a perinuclear location, or how is the symmetrical distribution regulated in order to ensure proper inheritance of the material by both daughter cells. It is also not clear whether there is a controlled mechanism that regulates the number of granules inherited by the daughter cells, or whether it is a random process. We describe the distribution of germ plasm material from 4hpf to 24hpf in zebrafish primordial germ cells using Vasa protein as marker. Vasa positive material appears to be conglomerate into 3 to 4 big spherical structures at 4hpf. While development progresses, these big structures become smaller perinuclear granules that reach a total number of approximately 30 at 24hpf. We investigated how this transformation occurs and how the minus-end microtubule dependent motor protein Dynein plays a role in this process. Additionally, we describe specific colocalization of microtubules and perinuclear granules during interphase and more interestingly, during all different stages of cell division. We show that distribution of granules follow what seems to be a regulated distribution: during cells division, daughter cells inherit an equal number of granules. We propose that due to the permanent colocalization of microtubular structures with germinal granules during interphase and cell division, a coordinated mechanism between these structures may ensure proper distribution of the material among daughter cells. Furthermore, we show that exposure to the microtubule-depolymerizing drug nocodazole leads to disassembly of the germ cell nuclear lamin matrix, chromatin condensation, and fusion of granules to a big conglomerate, revealing dependence of granular distribution on microtubules and proper nuclear structure.

Kraichnan–Leith–Batchelor similarity theory and two-dimensional inverse cascades

We study the scaling properties and Kraichnan–Leith–Batchelor (KLB) theory of forced inverse cascades in generalized two-dimensional (2D) fluids (α-turbulence models) simulated at resolution 8192x8192. We consider α=1 (surface quasigeostrophic flow), α=2 (2D Euler flow) and α=3. The forcing scale is well resolved, a direct cascade is present and there is no large-scale dissipation. Coherent vortices spanning a range of sizes, most larger than the forcing scale, are present for both α=1 and α=2. The active scalar field for α=3 contains comparatively few and small vortices. The energy spectral slopes in the inverse cascade are steeper than the KLB prediction −(7−α)/3 in all three systems. Since we stop the simulations well before the cascades have reached the domain scale, vortex formation and spectral steepening are not due to condensation effects; nor are they caused by large-scale dissipation, which is absent. One- and two-point p.d.f.s, hyperflatness factors and structure functions indicate that the inverse cascades are intermittent and non-Gaussian over much of the inertial range for α=1 and α=2, while the α=3 inverse cascade is much closer to Gaussian and non-intermittent. For α=3 the steep spectrum is close to that associated with enstrophy equipartition. Continuous wavelet analysis shows approximate KLB scaling ℰ(k)∝k−2 (α=1) and ℰ(k)∝k−5/3 (α=2) in the interstitial regions between the coherent vortices. Our results demonstrate that coherent vortex formation (α=1 and α=2) and non-realizability (α=3) cause 2D inverse cascades to deviate from the KLB predictions, but that the flow between the vortices exhibits KLB scaling and non-intermittent statistics for α=1 and α=2.

VELOCITY FIELD OF COMPRESSIBLE MAGNETOHYDRODYNAMIC TURBULENCE: WAVELET DECOMPOSITION AND MODE SCALINGS

We study compressible magnetohydrodynamic turbulence, which holds the key to many astrophysical processes, including star formation and cosmic-ray propagation. To account for the variations of the magnetic field in the strongly turbulent fluid, we use wavelet decomposition of the turbulent velocity field into Alfven, slow, and fast modes, which presents an extension of the Cho & Lazarian decomposition approach based on Fourier transforms. The wavelets allow us to follow the variations of the local direction of the magnetic field and therefore improve the quality of the decomposition compared to the Fourier transforms, which are done in the mean field reference frame. For each resulting component, we calculate the spectra and two-point statistics such as longitudinal and transverse structure functions as well as higher order intermittency statistics. In addition, we perform a Helmholtz-Hodge decomposition of the velocity field into incompressible and compressible parts and analyze these components. We find that the turbulence intermittency is different for different components, and we show that the intermittency statistics depend on whether the phenomenon was studied in the global reference frame related to the mean magnetic field or in the frame defined by the local magnetic field. The dependencies of the measures we obtained are different for different components of the velocity; for instance, we show that while the Alfven mode intermittency changes marginally with the Mach number, the intermittency of the fast mode is substantially affected by the change.

Weak nonmesonic decay spectra of hypernuclei

We compute one- and two-nucleon kinetic-energy spectra and opening-angle distributions for the nonmesonic weak decay of several hypernuclei, and compare our results with some recent data. The decay dynamics is described by transition potentials of the one-meson-exchange type, and the nuclear structure aspects by two versions of the independent-particle shell model (IPSM). In version IPSM-a, the bole states are treated as stationary, while in version IPSM-b the deep-hole ones are considered to be quasi-stationary and are described by Breit-Wigner distributions.

Understanding fusion suppression and enhancement in the (18)O+(58,60,64)Ni systems

Realistic coupled-channel calculation results for the (18)[O] + (58,60,64)Ni systems in the bombarding energy range 34.5 <= E(Lab) <= 6-5 MeV are presented. The overall agreement with existing experimental data is quite good. Our calculations predict an unexpected fusion suppression for above-barrier energies, with an important contribution of the two neutron ((18)O, (16)O) transfer channel couplings. The sub-barrier fusion enhancement and the above barrier suppression, predicted by the calculations, are consistent with the nuclear structure of the Ni region. Comparisons with recently reported similar effects in reactions induced by the (6)He projectile are discussed. (C) 2009 Elsevier B.V. All rights reserved.

Identificação não linear usando uma rede fuzzy wavelet neural network modificada

In last decades, neural networks have been established as a major tool for the identification of nonlinear systems. Among the various types of networks used in identification, one that can be highlighted is the wavelet neural network (WNN). This network combines the characteristics of wavelet multiresolution theory with learning ability and generalization of neural networks usually, providing more accurate models than those ones obtained by traditional networks. An extension of WNN networks is to combine the neuro-fuzzy ANFIS (Adaptive Network Based Fuzzy Inference System) structure with wavelets, leading to generate the Fuzzy Wavelet Neural Network - FWNN structure. This network is very similar to ANFIS networks, with the difference that traditional polynomials present in consequent of this network are replaced by WNN networks. This paper proposes the identification of nonlinear dynamical systems from a network FWNN modified. In the proposed structure, functions only wavelets are used in the consequent. Thus, it is possible to obtain a simplification of the structure, reducing the number of adjustable parameters of the network. To evaluate the performance of network FWNN with this modification, an analysis of network performance is made, verifying advantages, disadvantages and cost effectiveness when compared to other existing FWNN structures in literature. The evaluations are carried out via the identification of two simulated systems traditionally found in the literature and a real nonlinear system, consisting of a nonlinear multi section tank. Finally, the network is used to infer values of temperature and humidity inside of a neonatal incubator. The execution of such analyzes is based on various criteria, like: mean squared error, number of training epochs, number of adjustable parameters, the variation of the mean square error, among others. The results found show the generalization ability of the modified structure, despite the simplification performed

Cardioplegia using low volumic cardioplegic agents: morphological study in isolated rabbit hearts

INTRODUÇÃO: A solução cardioplégica farmacológica busca eliminar as conseqüências do dano isquêmico, que é o resultado do desbalanço entre a oferta e o consumo de energia durante a parada dos batimentos cardíacos, nas cirurgias cardíacas com circulação extracorpórea. OBJETIVO: Este trabalho avalia experimentalmente as alterações estruturais e ultra-estruturais em coração isolado de coelhos submetidos à parada protegida pela Solução para Cardioplegia de Baixo Volume (SCBV). MÉTODO: O estudo compreendeu um grupo controle e dois grupos experimentais. No grupo I, a parada cardíaca foi obtida pela infusão da SCBV por 2 horas. No grupo II, o experimento foi conduzido da mesma forma até a parada protegida pela SCBV por duas horas, imediatamente procedeu-se à reperfusão com solução oxigenada de Ringer Locke (RL) por uma hora. No grupo controle os corações foram perfundidos com solução oxigenada de RL por duas horas. Após os experimentos, oito amostras de parede lateral do ventrículo esquerdo foram fixadas em formaldeído 10% e glutaraldeído 2,5% para análises histológica e ultra-estrutural. RESULTADOS: As células do miocárdio, fibroblastos e células endoteliais, observadas nos grupos experimentais I e II, apresentaram marginalização da heterocromatina, compactação do nucléolo, alteração na forma das mitocôndrias, compactação das cristas e aumento da densidade da matriz mitocondrial, indicando que tanto a estrutura nuclear quanto a das organelas citoplasmáticas foram alteradas em relação às células do grupo controle. CONCLUSÃO: As modificações estruturais foram decorrentes de uma adaptação fisiológica da célula, não sendo indicativas de oncose ou apoptose, sugerindo, portanto, que a solução cardioplégica utilizada foi eficiente para a preservação das células.

Target mass corrections revisited

We propose a new implementation of target mass corrections to nucleon structure functions which, unlike existing treatments, has the correct kinematic threshold behavior at finite Q(2) in the x -> 1 limit. We illustrate the differences between the new approach and existing prescriptions by considering specific examples for the F-2 and F-L structure functions, and discuss the broader implications of our results, which call into question the notion of universal parton distribution at finite Q(2).

A systematization for one-loop 4D Feynman integrals

We present a strategy for the systematization of manipulations and calculations involving divergent (or not) Feynman integrals, typical of the one-loop perturbative solutions of QFT, where the use of an explicit regularization is avoided. Two types of systematization are adopted. The divergent parts are put in terms of a small number of standard objects, and a set of structure functions for the finite parts is also defined. Some important properties of the finite structures, specially useful in the verification of relations among Green's functions, are identified. We show that, in fundamental (renormalizable) theories, all the finite parts of two-, three- and four-point functions can be written in terms of only three basic functions while the divergent parts require (only) five objects. The final results obtained within the proposed strategy can be easily converted into those corresponding to any specific regularization technique providing an unified point of view for the treatment of divergent Feynman integrals. Examples of physical amplitudes evaluation and their corresponding symmetry relations verification are presented as well as generalizations of our results for the treatment of Green's functions having an arbitrary number of points are considered.

IMPROVED STATISTICAL QCD MODEL FOR THE QUARK CONTENT of THE NUCLEON

The neutron-to-proton ratio of the structure functions, F(2)(n)/F(2)(p), as well as the corresponding difference F(2)(p)-F(2)(n) are obtained within a statistical quark model for the nucleon, where the quark energy levels are given by a central linear confining potential.

Weak nonmesonic decay spectra of hypernuclei

We compute one- and two-nucleon kinetic-energy spectra and opening-angle distributions for the nonmesonic weak decay of several hypernuclei, and compare our results with some recent data. The decay dynamics is described by transition potentials of the one-meson-exchange type, and the nuclear structure aspects by two versions of the independent-particle shell model (IPSM). In version IPSM-a, the bole states are treated as stationary, while in version IPSM-b the deep-hole ones are considered to be quasi-stationary and are described by Breit-Wigner distributions.

Kinetic energy sum spectra in nonmesonic weak decay of hypernuclei

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

ULTRASTRUCTURE OF THE GERMINATIVE EPITHELIUM IN A GOAT WITH A 5/15-ROBERTSONIAN TRANSLOCATION

The spermatogenesis of two goats bearing a 5/15 Robertsonian translocation was investigated by electron microscopy. There was no dramatic change in the morphology of the cells of the spermatic line. All cells of the seminiferous epithelium seemed quite normal at the ultrastructural level. However a certain disturbance in the cell localization and some morphological abnormalities involving nuclear structure were seen. Spermatocytes and spermatids normal in appearance were observed, but a great number of cells presented two or more nuclei. These cells were frequently seen to become degenerated during spermatogenesis. We believe that unbalanced spermatocytes degenerate during the process and only some spermatocytes succeed in fertilizing gametes.

The surface geometry of exotic nuclei

We analyze the surface geometry of the spherical even-even Ca, Ni, Sn and Pb nuclei using two approaches: The relativistic Dirac-Hartree-Bogoliubov one with several parameter sets and the non-relativistic Hartree-Fock-Bogoliubov one with the Gogny force. The proton and neutron density distributions are fitted to two-parameter Fermi density distributions to obtain the half-density radii and diffuseness parameters. Those parameters allow us to determine the nature of the neutron skins predicted by the models. The calculations are compared with existing experimental data. © 2007 American Institute of Physics.

Nonmesonic weak decay of Λ-hypernuclei within independent-particle shell-model

After a short introduction to the nonmesonic weak decay (NMWD) ΛN→nN of Λ-hypernuclei we discuss the long-standing puzzle on the ratio Γn/Γp, and some recent experimental evidences that signalized towards its final solution. Two versions of the Independent-Particle-Shell-Model (IPSM) are employed to account for the nuclear structure of the final residual nuclei. They are: (a) IPSM-a, where no correlation, except for the Pauli principle, is taken into account, and (b) IPSM-b, where the highly excited hole states are considered to be quasi-stationary and are described by Breit-Wigner distributions, whose widths are estimated from the experimental data. We evaluate the coincidence spectra in Λ 4He, Λ 5He, Λ 12C, Λ 16O, and Λ 28Si, as a function of the sum of kinetic energies EnN=En+EN for N=n, p. The recent Brookhaven National Laboratory experiment E788 on Λ 4He, is interpreted within the IPSM. We found that the shapes of all the spectra are basically tailored by the kinematics of the corresponding phase space, depending very weakly on the dynamics, which is gauged here by the one-meson-exchange- potential. In spite of the straightforwardness of the approach a good agreement with data is achieved. This might be an indication that the final-state- interactions and the two-nucleon induced processes are not very important in the decay of this hypernucleus. We have also found that the π+K exchange potential with soft vertex-form-factor cutoffs (Λπ≈0. 7GeV, ΛK≈0.9GeV), is able to account simultaneously for the available experimental data related to Γp and Γn for Λ 4H, and Λ 5He. © 2010 American Institute of Physics.