Surface microbial consortia from Livarot, a French smear-ripened cheese

The surface microflora (902 isolates) of Livarot cheeses from three dairies was investigated during ripening. Yeasts were mainly identified by Fourier transform infrared spectroscopy. Geotrichum candidum was the dominating yeast among 10 species. Bacteria were identified using Biotype 100 strips, dereplicated by repetitive extragenic palindromic PCR (rep-PCR); 156 representative strains were identified by either BOX-PCR or (GTG) 55-PCR, and when appropriate by 16S rDNA sequencing and SDS-PAGE analysis. Gram-positive bacteria accounted for 65% of the isolates and were mainly assigned to the genera Arthrobacter, Brevibacterium, Corynebacterium, and Staphylococcus. New taxa related to the genera Agrococcus and Leucobacter were found. Yeast and Gram-positive bacteria strains deliberately added as smearing agents were sometimes undetected during ripening. Thirty-two percent of the isolates were Gram-negative bacteria, which showed a high level of diversity and mainly included members of the genera Alcaligenes, Hafnia, Proteus, Pseudomonas, and Psychrobacter. Whatever the milk used (pasteurized or unpasteurized), similar levels of biodiversity were observed in the three dairies, all of which had efficient cleaning procedures and good manufacturing practices. It appears that some of the Gramnegative bacteria identified should now be regarded as potentially useful in some cheese technologies. The assessment of their positive versus negative role should be objectively examined.

Manipulatív marketing: fogyasztói észlelések és reakciók

A marketing koncepciónak kezdetektől fogva a vevőorientáció az egyik kulcsfogalma. Tulajdonképpen annak a szemléletváltásnak köszönheti felemelkedését és más menedzsmentdiszciplínától való elhatárolását is, amellyel a vevői igényeket helyezte a vállalati gondolkodás középpontjába. Későbbiekben azonban a kutatók rájöttek arra, hogy maguk az igények az üzleti gyakorlat során nagymértékben kontrollálhatók, és az eladói oldal azóta is él ezekkel a lehetőségekkel. A kutatás arra volt kíváncsi, hogy a vállalatok manipulációs gyakorlata hogyan csapódik le a fogyasztókban, melyek azok a manipulációs eszközök, amelyeket észlelnek, és azokra milyen válaszreakciókat adnak. Azonosíthatók-e tipikus gondolkodási sémák, magatartási mintázatok, amelyek jól jellemzik egyes vevők viszonyát a vállalati manipulációval. A tanulmány feltáró interjúkra épít, amely segít megérteni a fogyasztókban végbe menő komplex mechanizmusokat. Az eredmények azt sugallják, hogy kétfajta folyamat játszódik le a vevői oldalon Egyrészt a fogyasztók idővel rutinszerű magatartásmintákat vesznek fel, amely során a manipulációs eszközök érvényesülése nem tudatos, és a vállalatoknak kedvező reakciókat vált ki. Másrészt, a fogyasztói társadalom is egyfajta érési folyamaton megy át, aminek hatására megtanulják a vállalatok gondolkodásmódját, és – bár a kognitív korlátok miatt azokat nem tudják teljes mértékben lekövetni – igyekeznek önvédő heurisztikákat beépíteni a követett magatartásukba. A kutatás alapján hipotetikusan öt vevőtípust azonosítottunk, akiket egymástól eltérő reakciók jellemeznek. Customer orientation has been the key concept of marketing since the beginning. As a matter of fact, the emergence of marketing and the conceptual distinction between marketing and the related management disciplines can be attributed to this new approach, which put customer needs in the focus of company attention. Later, researchers revealed that the needs themselves can be controlled to a greater extent and sellers have been taking advantage of it since then. The main focus of the research concentrated on the effects of manipulation practices of companies on consumers, which manipulation tools are recognised and how consumers react to them. Furthermore the research also investigated whether typical cognitive, emotional and behavioural patterns, which reflect the relationship between consumers and the manipulation practices of companies, can be identified. The study relies on explorative interviews, which help understand the complex mechanisms of consumer attitudes. The results indicates that the opposite process occur on the consumer side. On the one hand, consumers tend to follow behavioural routines after a while, which help manipulation tools work in an unconscious way that triggers reactions for the favour of companies. On the other hand, the generations of consumers are getting more and more mature and learning the companies’ mentality and, despite having cognitive limitations to identify all the tricks and deceptive attempts, they try to develop and follow defensive heuristics. Based on the data explored we identified five customer types and we described their diverse reactions to manipulation.

Relação universidade-escola: o PIBID como instrumento de intervenção sobre o real da formação de professores

The theme of teacher education has always been rich in discussion and presents an abundant literature on the subject. Historically this topic has generated concerns in both development bodies and universities / schools where these people learn or are engaged in professional work. Training teachers is complex and these elements of complexity make necessary a review of paradigms of initial and continuing education. Despite the efforts of the past decades, the lack of teachers in some areas of knowledge is still a big concern, and it can become even worse in the future, what reinforces the importance of new decisions and new directions in order to change this situation. Therefore, the university-school relationship is of fundamental importance, linking and articulating theory and school practice, contextualizing knowledge, renewing and adapting curricula to current times and spaces in order to be able to improve and recover the social and professional value of teachers. From this perspective the education public policies should turn to the encouragement and the rescue of values and principles in quality teacher training. In the course comes the Institutional Teaching Initiation Scholarships Program - PIBID as an innovative program of teacher education working and adding essential factors to the university-school to reinforce good teaching practices taking up the role of co-developer schools. This research is aimed at analyzing the factors that PIBID inserts in the university-school relationship within IFPR Campus Palmas. The theoretical route was marked by authors as Edgar Morin (2003, 2010a, 2010b, 2012), Enrique Leff (2002a, 2002b, 2003, 2010), Boaventura Sousa Santos (1988, 2010a, 2010b, 2013) Menga Lüdke (2005, 2013), Demerval Saviani (2000, 2013), Paulo Freire (2011), among others, among them official documents of PIBID were used in this research too. The methodological approach with exploratory approach, descriptive explanatory was of fundamental importance through data collected by the documentary analysis (BRAZIL, 2007, 2009, 2013) and in the focus groups activities (GATTI, 2012). The focus groups interlocutors constituted of three groups: Area Coordinators, supervisors and teaching initiation scholarships. The categories were defined a priori from the Programme's objectives and emerging categories identified from the analysis process. After both documentary and interlocutors analysis, it was possible to identify that PIBID inserts the following factors in the university-school relationship: the Recognition of the Profession; Innovative Program and Dialogues of Knowledge. For the recognition of the profession mainly because it is an initial and continuing education program; it approximates theory and practice; upgrades the role of the teacher at school and motivates methodological innovations. This Innovative Program promotes the role of co-educational school and it also approximates knowledge of the school reality and promotes the continuous training. The third emerging category university-school relationship promotes dialogs of knowledge; bringing together theory and practice; it allows information exchange and opens new perspectives for teacher training. Finally, it is possible to realize that besides being a new program, PIBID has promoted visible changes through the actions carried out by all subprojects in partnerships between universities and schools, restoring and giving new meanings to the pedagogical practices.

Minas e memórias da Urgeiriça: contributos da arte para a sensibilização ambiental

O projeto "Minas e Memórias da Urgeiriça" focaliza-se nos contributos da arte enquanto vetor de sensibilização ambiental, entendida como um veículo privilegiado para promover comportamentos e atitudes mais sustentáveis, responsáveis e cívicas, apelando para a mudança ou para o reforço de boas práticas ambientais. O projeto foi implementado no Concelho de Nelas, distrito de Viseu, baseando-se na história socioambiental das Minas da Urgeiriça, localizadas na freguesia de Canas de Senhorim. Abarcou um conjunto de atividades de índoles pedagógico e artístico, que se interligaram coerentemente, iniciando-se com o seminário “Urgeiriça: Antes, Agora e Depois?”, seguindo-se a performance “Escuridão” complementada pelos cantares de hinos dos ex-trabalhadores das Minas da Urgeiriça, culminando na visita à instalação artística “Escavações”. Os resultados estão ancorados em duas premissas fundamentais: por um lado, os testemunhos reais dos ex-trabalhadores mineiros expressaram a influência da ação do homem sobre a natureza de forma descomedida e irresponsável, que continua a acarretar consequências nefastas para o ser humano, e por outro, demonstraram as intervenções a que o local se sujeitou (da exploração desenfreada de recursos até à progressiva requalificação). Para tal, conseguiu-se revitalizar e reforçar as memórias do espaço (físico e mental) e materializá-las através da arte como forma de sensibilizar a comunidade local acerca das ações resultantes da interação do homem versus ambiente, sendo encaradas como meio privilegiado de desenvolvimento comunitário, gerador de competências e de mudanças.

Odpowiedzialność lekarza, podmiotu leczniczego i ubezpieczyciela za nieudane transplantacje

Transplantacje komórek, tkanek i narządów należą do najbardziej kontrowersyjnych procedur medycznych, gdyż dotykają także kwestii etycznych i światopoglądowych. W ostatnich latach niewątpliwie obserwujemy rozwój transplantologii dzięki postępowi w naukach medycznych oraz zmianach w świadomości społeczeństwa. Transplantologia pozostaje jedną z bardziej ryzykownych gałęzi medycyny, ponieważ postępowanie zgodnie z zasadami sztuki lekarskiej nie jest gwarancją powodzenia przeszczepu. Artykuł przybliża problematykę nieudanych transplantacji – zarówno ze względu na zaniedbania lekarza czy placówki medycznej, jak i ze względu na reakcję obronną organizmu. W drugiej części artykułu zostały opisane zasady odpowiedzialności cywilnej lekarzy i innych pracowników podmiotów leczniczych za szkody wyrządzone pacjentom oraz wskazano czym różni się odpowiedzialność sprawcy szkody od odpowiedzialności ubezpieczyciela. Kolejna część artykułu dotyczy specyfiki odpowiedzialności ubezpieczyciela za nieudane transplantacje w oparciu o rozporządzenie Ministra Finansów w sprawie obowiązkowego ubezpieczenia odpowiedzialności cywilnej podmiotów wykonujących działalność leczniczą.

Construção de um modelo lógico do plano ABC (Agricultura de Baixa Emissão de Carbono) como uma proposta de avaliação

Dissertação (mestrado)—Universidade de Brasília, Faculdade de Agronomia e Medicina Veterinária, Programa de Pós-Graduação em Agronegócios, 2016.

MICROBIOLOGICAL ANALYSIS OF TRADITIONALLY FERMENTED MILK SOLD IN KINIGI SECTOR OF MUSANZE DISTRICT IN RWANDA

A research work entitled: “Microbiological analysis of traditionally fermented milk (Ikivuguto) sold in Kinigi Sector of Musanze District,” was carried out at Higher Learning Institution of Applied Sciences (INES-Ruhengeri) Laboratory of Microbiology located near Volcanoes in the Northern Province of Rwanda. The main objective of this work was to determine the microbiological quality of traditionally fermented milk, which is consumed by Kinigi Center local people. The hypothesis was to analyze if traditionally fermented milk commercialized in Kinigi restaurants contained pathogenic bacteria such as fecal coliforms and Escherichia coli , in addition to staphylococci and yeasts. Milk samples were collected from Kinigi sector and examined in the microbiology laboratory in order to assess the microbiological quality and safety of traditionally fermented milk in rural areas. The samples were analyzed qualitatively and quantitatively for the microbes found in fermented milk sold in Kinigi Center, and the results were as follows: 7.21x107 CFU/ml for total counts; 3.89x107 CFU/ml for Lactobacillus ; 2.77x107 CFU/ml for yeasts; 1.196x105 CFU/ml for total coliforms; 9.63x104 CFU/ml for fecal coliforms and 8.92x103 CFU/ml for staphylococci. Biochemical tests were carried out and the results showed that identified pathogens were E. coli, Providencia alcalifaciens , and the staphylococci group. It was found that fermented milk contained genera and species of Staphylococcus haemolyticus , Staphylococcus aureus , Staphylococcus intermedius , Staphylococcus xylosus and Staphylococcus saprophyticus . Findings showed that the commercial milk samples were cross-contaminated by different pathogens from environment. These contaminations could have been due to improper handling, presence of flies, soil erosion, dust from atmosphere, as well as contaminated milk vessels or pots, stirrers and unpasteurized water. It was concluded that local farmers and milk retailers did not adhere to required hygienic conditions for milk safety. In this regard, the sold traditional fermented milk does not meet health and safety standards because people did not respect good manufacturing practices. The hypothesis and main objective were confirmed, because traditionally fermented milk of Kinigi was cross-contaminated before consumption. Thus, it would be better to train farmers in the areas of product hygiene, sanitation and safety during milking, processing and marketing.

The impact of Portuguese demographic changes on the demand and supply of blood in the region of Algarve

Dissertação de Mastrado, Gestão de Unidades de Saúde, Faculdade de Economia, Universidade do Algarve, 2016

Corporate governance : value creation and performance in credit unions

In this study, we examine the relationship between good corporate governance practices and the creation of value/performance of credit unions from 2010 to 2012. The objective was to create and validate a corporate governance index for credit unions, and to then analyse the relationship between good governance practices and the creation of value/performance. The problem question is: do good corporate governance practices provide value creation for credit unions? The research started by creating indices from factor analysis to identify latent dependent variables related to value creation and performance; next indices were created from the principal component analysis for the creation of independent latent variables related to corporate governance. Finally, based on panel data from regression models, the influence of the variables and indices related to corporate governance on the indices of value creation and performance was verified. Based on the research, it became evident that the Corporate Governance Index (IGC) is mainly impacted by Executive Management, with 40.31% of the IGC value, followed by the Representation and Participation dimension, with 34.07% of the IGC value. The contribution for academics was the creation of the Corporate Governance Index (IGC) applied for credit unions. As for the contribution to the system of credit unions, the highlight was the effectiveness of the mechanisms for economic-financial and asset management adopted by BACEN, credit unions and OCEMG.

Tratamento de diarreias neonatais em vitelos

Dissertação de Mestrado Integrado em Medicina Veterinária

Evaluación de la exposición al ruido industrial en los trabajadores de una planta de asfalto, en la provincia del Azuay y propuesta de plan de control

El presente trabajo se realizó en una Planta de Hormigón Asfáltico, donde se realizó el estudio del ruido como factor de riesgo bajo las perspectivas de: Salud Ocupacional, Seguridad e Higiene Industrial. Este enfoque holístico, define la influencia que éste riesgo laboral ejerce sobre la pérdida de agudeza auditiva. Se estableció inicialmente el marco teórico y legal vigente sobre el ruido, posteriormente se describió la metodología de estudio, operatividad de variables, y la muestra a estudiar. Luego se procedió a la caracterización de la población y área estudiada, así como las actividades productivas. Dentro de la Higiene Industrial, se monitoreó la exposición del nivel de presión sonora, se realizó el comparativo con el nivel permisible (TWA 8 horas) establecido en el “Reglamento de Seguridad y Salud de los Trabajadores”, D.E. 2393. En términos de Seguridad Industrial, se analizó el equipo de protección personal EPP utilizado, y las variables determinantes. En el ámbito de Salud Ocupacional, se estudió: características personales de trabajadores, patologías relacionadas con sordera, y un cuestionario de exposición al ruido. Finalmente se determinó la existencia de sobreexposición a ruido laboral en la empresa objeto de estudio, mediante el estudio del estado de salud auditiva de los colaboradores se determinó la gran incidencia de personal sano y finalmente se determinó las medidas de control a implementarse enfatizadas a la realidad descubierta en el presente estudio, las que incluyen cambios en equipos y maquinaria, buenas prácticas de trabajo, planes de adiestramiento y capacitación en todo el personal entre otros.

Eutrofização e qualidade da água na piscicultura: consequências e recomendações

The eutrophication (cultural or anthropogenic) is induced by man and can have different origins, such as domestic sewage, industrial and agricultural activities, including the effluent still breeding systems of aquatic organisms. The expansion of aquaculture, with production of biomass and increase of nutrients in water may cause acceleration of productivity of algae, changing the ecology of aquatic systems. In addition, these waste water may present a risk to health through the transfer of pathogens from manure, plant residues, composted material, among others that are major sources of organic waste in some farming systems. Depending on the trophic level of fish ponds, which are dynamic environments, different planktonic species with short reproductive cycle and adapted to the changes contained in these systems can appear in high abundance. Water quality in the systems for raising fish is related to several factors, such as water source, management (liming, fertilizing, cleaning), cultivated species and quantity and composition of exogenous food. In order to minimize environmental impacts, there are techniques to improve the quality of water in fish farming systems and thus satisfactory answers can be obtained through the application of management practices. This paper aims to review the subject that deals with changes in water quality resulting from the activity of freshwater fish culture in Brazil. Search also recommend techniques of good management practices to minimize the impact generated by the activity.

Avaliação dos principais programas de boas práticas agropecuárias implantados no Brasil para promover a sustentabilidade da cadeia leiteira

Dissertação (mestrado)—Universidade de Brasília, Faculdade de Agronomia e Medicina Veterinária, 2016.