1000 resultados para digestibilidade proteica
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To evaluate the nutritional value of palm kernel meal on the performance Nile tilapia (Oreochromis niloticus), five isonitrogenous (30% crude protein), isoenergetic (2800 kcal/kg of digestible energy), and isofibrous (10% crude fiber) diets, with increasing levels of palm kernel meal (0, 7, 14, 21, 28 and 35%). were ad libitum fed to fingerlings with initial body weight of 1.52g +/- 0.04, housed per 120 days in 35 aquarium of 60 liters with fingerlings each. The environmental culture condition were monitored during all experimental period. Statistical analyses of recorded data were performed through polynomial regression models. To determine the apparent digestibility, macroscopic analysis of viscera and microscopic analysis of intestine epithelium were performed. The obtained results indicated that the inclusion of palm kernel meal up to 35% Nile tilapia diets did not affects the apparent digestibility and did not induce any pathological effects on viscera and intestinal epithelium.
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An experiment was performed in order to evaluate the beta-glucuronidase activity in gastric juice and gastric mucosa of rats submitted to a protein-free diet. A group of 36 young adult male Wistar rats was fed a protein-free diet ad libitum for five weeks; a second group of 36 Wistar rats ingested a purified isocaloric 12,5% casein diet for the same period. The concentration of proteins in plasma, gastric juice and gastric glandular mucosa and the beta-glucuronidase activity in the gastric juice and gastric glandular mucosa were determined. Protein deficient rats had lower plasma protein concentrations and also lower protein concentrations in gastric juice and gastric mucosa. In these animals there was no significant change of beta-glucuronidase activity in the gastric juice, but there was a significant increase of the specific enzymatic activity in the gastric mucosa. The results suggest that protein restriction in young adult rats affects the gastric mucosa. The increase of the specific beta-glucuronidase activity might be due to heightened local catabolism or to a comparatively more severe protein depletion.
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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The intake, the apparent digestibility and the nutritive value of elephant grass (Pennisetum purpureum Schum.) silages prepared with the addition of 0, 8, 16 and 24% of ground ear corn with husks, wheat bran and saccharin, dry weight of additive/wet weight of green chop upon the silage were evaluated. A randomized block design with three replications, in a factorial arrangement (3 additives x 4 levels) was used. As experimental silos, 200-liter plastic vessels were used. Sheep weighing approximately 50 kg, kept in individual cages, receiving water and mineral mixture ad libitum, were used to measure the intake and apparent digestibility of silages. There was a ten-day period of adaptation to the experiment conditions. The voluntary intake of the silages was determined by the mean of the intake observed in the last three days of a ten-day period. The fecal collection period lasted for seven days. In this period the animals were fed 80% of the observed intake obtained in the previous phase. The dry matter intake increased as the levels of the additives in the silages were increased. The digestibility of the wall cell components decreased as the rates of the additives in the ensilage process increased. The silages prepared with wheat bran or ground ear corn with husks showed higher nutritive value than the ones with saccharin.
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Two trials were conducted aiming to of evaluate the effect of probiotic supplementation in pig's diet: Trial 1: diarrhea incidence and performance; Trial 2: feed intake and digestibility. In the Trial 1, forty weaning barrow piglets were distributed in four treatments: T0-basal diet; T100-basal diet +100ppm of probiotic; T200-basal diet +200ppm e T300-basal diet +300ppm. The trial 2 was a digestibility trial, where eight barrow pigs were used, distributed in two treatments: T1-basal diet and T2-basal diet + 200 ppm of same probiotic used in the Experiment 1. In the Period 1 the animals of T0 and T100 groups showed higher diarrhea incidence (P<0.05) than the T200 and T300 groups. The performance of animals of T100 group were lower than other treatment groups (P<0.05). In the Period 2 the T200 and T300 animals, showed better FG ratio than the animals of T0 (P<0.05). In the total period it was not observed significative difference concerning performance, except for DFI. In the trial 2, the animals of treatment T2, showed an increase of feed intake when compared with animals of T1. Digestibility coefficients showed no significative differences among treatments. It was concluded that the addition of 200 and 300 ppm of probiotic in the Period 1 reduces incidences of diarrhea. But, in the total period of trial 1, the performance was similar among treatments. In the trial 2 it was observed better adaptation of animals receiving probiotics which was represented by higher feed intake.
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Twelve elephant grass genotypes were evaluated in a randomized blocks design with three repetitions. The experimental parcel was of 4 lines with 3m of length, spaced of 1 m and fertilized with 100 kg of P2O5, 100 kg of N, 60 kg of K2O and 25 kg of micronutrients/ha. Significant differences were observed among the genotypes for neutral detergent fiber (FND), acid detergent fiber (FAD) and lignin. The genotypes CNPGL 92-70-02, CNPGL 91-25-01 and CNPGL 93-32-02 showed low values for FND, FAD and lignin, simultaneously, outstanding between the others. There were no significant differences in the levels of dry matter and crude protein contents and in in vitro dry matter digestibility between the genotypes.
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The objective of this work was to evaluate the effect of nitrogen sources, using sugarcane supplemented with urea, corn gluten meal-60 or soybean meal. It was evaluated Intake, total and partial apparent digestibility were evaluated using four rumen and duodenal cannulated crossbred steers. The treatments were evaluated in a 4 × 4 Latin square design. The total feces were collected to determine the apparent digestibility on the total intestinal tract. The indigestible acid detergent fiber (iADF) was used as marker of duodenal flux to determine ruminal and intestinal digestibility and dry matter daily flux. The DM, OM, CP, EE, DE, NDF, ADF and TC intakes and digestibility rates were influenced by nitrogen sources, and lower intake was observed in animals fed exclusively with cane sugar compared to animals fed the other experimental diets. Meanwhile the sources of nitrogen did not affect the total digestible nutrients (TDN) values. The RDP sources did not affect the total, ruminal, and intestinal nutrients apparent digestibility. However, the total, ruminal, and intestinal dry matter, and protein apparent digestibility were affected by the RDP sources.
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They were compared the coefficients of apparent digestibility (CAD) and the fecal quality of dogs fed a home-made diet and two dog foods, standard and super-premium. Six adult dogs were distributed in a double latin square desing (3 × 3), with three treatments and three periods, in a total of six replications per treatment. Tukey's test was used to compare the means. The home-made diet presented the highest CAD, not differing only of the CAD of acid ether extract of the super-premium food. The standard food was the lowest digestive, producting more feces. Fecal dry matter of dogs fed home-made diet was lower than in the standard and super-premium foods, which did not differ among then. Fecal score presented low variability among treatments, remaining in the considered ideal range. Home-made diet can be an alternative in the feeding of dogs.
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This study evaluated the effect of supplementation and the type of supplement on nutrient digestibility and performance of lactating calves fed with Brachiara brizantha cv. Marandu pasture from February to May. The treatments were: supplementation in creep-feeding with protein concentrate, with energy concentrate, with energy-protein concentrate and without concentrate. In a Latin square design, four Canchim calves were kept in individual pens, receiving marandu hay and controlled suckling twice a day. The performance was evaluated in a completely randomized design, with 23 calves kept with the cows. Higher intake and nutrients digestibility (65% of dry mater digestibility) and calf performance (live weight gain of 0.98 kg day-1) were obtained with energyprotein concentrate, and the cows kept weight and body reserves during the experiment. The protein concentrate did not show good results, and was lower than the performance of calves without supplementation - 0.56 and 0.77 kg day-1, respectively. The performance of calves without supplementation was similar to calves receiving energy concentrate, but the cows lost more body weight and energy reserves. The supplementation with energy-protein concentrate for lactating calves fed with Brachiara brizantha cv. Marandu during the beginning of the dry season was benefifical for the performance of calves and cows.
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This work aimed to evaluate the effect of increasing dietary fiber levels on the digestibility and gastrointestinal transit time (GTT) for pacu. The digestibility trial used 288 pacu in a completely randomized design, fed in aquaria and transferred to a modified Guelf-type feces collector, using the partial sampling method. The inert marker on the diets was chromic oxide (1%) to establish the diet digestibility. In the GTT assay, 288 pacu were distributed in 24 aquaria in a completely randomized design. Fish were fed with two colored diets - green and white - and feces color was determined after abdominal pressure. The white and green diets used 1% of titanium and chromic oxides, respectively. Total GTT was determined when all fish showed green feces. The apparent digestibility coefficients and GTT of diets decreased as dietary fiber levels increased. These results indicated that 9% dietary fiber can be used in pacu diets without negative effects on energy, protein, crude fiber and ether extract digestibility and GTT.
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This study aimed to determine the activities of trypsin inhibitors, hemagglutinant and tannin levels in soybean meal and in raw and processed soy, as well evaluate the protein apparent digestibility coefficient for pacu juveniles. The apparent coefficients of raw, extruded, toasted and milled soy were determined using chromium oxide (0.5%) as marker. A reference diet was created with 26% crude protein and 4,352 kcal kg-1, with each feed containing 30% of the test diet. Feces were collected by abdominal pressure. All analyzed products presented anti-nutritional factors, but the lowest trypsin inhibitor activity was observed in soybean meal. Soy that received thermal treatment presented better digestibility coefficients and lower hemagglutinating activity values than raw soy. No effects of trypsin and tannin inhibitor were observed on the protein digestibility coefficient, but a negative relationship was observed between hemagglutinin levels and protein digestibility coefficient. The use of soybean meal and extruded or toasted soy is recommended for pacu feeding.