992 resultados para Variety of speechs


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"A bibliography of William Makepeace Thackeray in the United States": v. 2, p. [223]-399.

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Multicellular tumor spheroids (MCTS) are used as organotypic models of normal and solid tumor tissue. Traditional techniques for generating MCTS, such as growth on nonadherent surfaces, in suspension, or on scaffolds, have a number of drawbacks, including the need for manual selection to achieve a homogeneous population and the use of nonphysiological matrix compounds. In this study we describe a mild method for the generation of MCTS, in which individual spheroids form in hanging drops suspended from a microtiter plate. The method has been successfully applied to a broad range of cell lines and shows nearly 100% efficiency (i.e., one spheroid per drop). Using the hepatoma cell line, HepG2, the hanging drop method generated well-rounded MCTS with a narrow size distribution (coefficient of variation [CV] 10% to 15%, compared with 40% to 60% for growth on nonadherent surfaces). Structural analysis of HepG2 and a mammary gland adenocarcinoma cell line, MCF-7, composed spheroids, revealed highly organized, three-dimensional, tissue-like structures with an extensive extracellular matrix. The hanging drop method represents an attractive alternative for MCTS production, because it is mild, can be applied to a wide variety of cell lines, and can produce spheroids of a homogeneous size without the need for sieving or manual selection. The method has applications for basic studies of physiology and metabolism, tumor biology, toxicology, cellular organization, and the development of bioartificial tissue. (C) 2003 Wiley Periodicals, Inc.

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We report genetic characterization of isochromosome 18p using a combination of cytogenetic and molecular genetic methods, including multiplex fluorescent PCR. The patient was referred for chorionic villus sampling (CVS) due to advanced maternal age and maternal anxiety. The placental karyotype was 47,XX,+mar, with the marker having the appearance of a small supernumerary isochromosome. Because differentiating between isochromosomes and other structural rearrangements is normally very difficult, a variety of genetic tests including fluorescence in situ hybridization (FISH), PCR, and multiplex fluorescent PCR were undertaken to determine chromosomal origin and copy number and, thus, allow accurate diagnosis of the corresponding syndrome. FISH determined that the marker chromosome contained chromosome 18 material. PCR of a variety of short tandem repeats (STRs) confirmed that there was at least one extra copy of the maternal 18p material. However, neither FISH nor PCR could accurately determine copy number. Multiplex fluorescent PCR (MF-PCR) of STRs simultaneously determined that: (1) the marker included 18p material; (2) the marker was maternal in origin; (3) allele copy number indicated tetrasomy; and (4) contamination of the sample could be ruled out. Results were also rapid with accurate diagnosis of the syndrome tetrasomy 18p possible within 5 hours.

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We describe a polygeneration system that can run on neat plant oils, such as Jatropha and Pongamia, or standard diesel fuel. A prototype has been constructed using a compression ignition engine of 9.9 kW shaft output. It consumes 3 L/h of fuel and will produce 40 kg/h of ice by means of an adsorption refrigerator powered from the engine jacket heat. Steaming of rice, deep and shallow frying, and other types of food preparation heated by the exhaust gas have been demonstrated. In addition, the feasibility of producing distilled water by means of multiple-effect distillation powered by the engine waste heat is shown. Overall plant efficiency and potential savings in greenhouse gas emissions are discussed. © 2012 Elsevier Ltd.

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2000 Mathematics Subject Classification: Primary: 17A32; Secondary: 16R10, 16P99, 17B01, 17B30, 20C30

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The topic was chosen because of a simple fact that there are more Chinese studying English in China than U.S. Americans who speak English. The aim of this paper is to demonstrate the unique linguistic features that make the Chinese use English in a particular style. First, it shows the development of the English language used by the Chinese: Chinese Pidgin English, Chinglish, Chinese English and China English. Second, it takes a deeper look into certain linguistic features regarding the English language used by the Chinese: lexical features morphosyntactic features and pragmatic features

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La programmation par contraintes est une technique puissante pour résoudre, entre autres, des problèmes d’ordonnancement de grande envergure. L’ordonnancement vise à allouer dans le temps des tâches à des ressources. Lors de son exécution, une tâche consomme une ressource à un taux constant. Généralement, on cherche à optimiser une fonction objectif telle la durée totale d’un ordonnancement. Résoudre un problème d’ordonnancement signifie trouver quand chaque tâche doit débuter et quelle ressource doit l’exécuter. La plupart des problèmes d’ordonnancement sont NP-Difficiles. Conséquemment, il n’existe aucun algorithme connu capable de les résoudre en temps polynomial. Cependant, il existe des spécialisations aux problèmes d’ordonnancement qui ne sont pas NP-Complet. Ces problèmes peuvent être résolus en temps polynomial en utilisant des algorithmes qui leur sont propres. Notre objectif est d’explorer ces algorithmes d’ordonnancement dans plusieurs contextes variés. Les techniques de filtrage ont beaucoup évolué dans les dernières années en ordonnancement basé sur les contraintes. La proéminence des algorithmes de filtrage repose sur leur habilité à réduire l’arbre de recherche en excluant les valeurs des domaines qui ne participent pas à des solutions au problème. Nous proposons des améliorations et présentons des algorithmes de filtrage plus efficaces pour résoudre des problèmes classiques d’ordonnancement. De plus, nous présentons des adaptations de techniques de filtrage pour le cas où les tâches peuvent être retardées. Nous considérons aussi différentes propriétés de problèmes industriels et résolvons plus efficacement des problèmes où le critère d’optimisation n’est pas nécessairement le moment où la dernière tâche se termine. Par exemple, nous présentons des algorithmes à temps polynomial pour le cas où la quantité de ressources fluctue dans le temps, ou quand le coût d’exécuter une tâche au temps t dépend de t.

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The paper evaluates the effects of organic and inorganic fertilizers on the growth of okra (variety NH-Ae 47-4. Organic fertilizers (cow dung and poultry droppings) and inorganic (NPK 15:15:15 and Urea 64:0) fertilizers were used for the experiment. The fertilizers were weighed and applied at 5g, 10g and 15g and were replicated three times and each having a control. Vegetative growth parameters taken include, shoot fresh weight (g), dry weight (g), plant height (cm), leaf number, stem girth (cm), leaf area (cm2). The results obtained from the experiment showed that the effect of the treatments were significantly difference from the control for all the parameters accessed with urea fertilizer having least effect. Plants treated with poultry litters have best performance by recording the highest fresh and dry weight (0.39g) at 4 weeks after planting (WAP); highest stem height 29.33cm for all the concentrations applied. Similarly, it has highest leaf area and stem girth (64.67cm2and 2.23cm respectively) at 8WAP.

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)