Energy consumption of European and African shrews

Is the extremely high oxygen consumption of shrews due to an unusually high basal metabolism? In an attempt to answer this long-standing question, we have measured the oxygen consumption of 13 species of shrews of different origin: from Europe - Sorex araneus, S. Minutus, Neomys fodiens, Crocidura russula, and Suncus etruscus; from Africa - Crocidura bottegi, C. bicolor, C. jouvenetae; C. poensis, C. theresae, C. Wimmeri, C. flavescens, and C. giffardi, The measurements, taken over a period of 20-30 minutes, were made in small, closed-system chambers at 25°C. The metabolic rat our shrews of the subfamily Soricinae lies between the eman and minimum values of the Soricini (M=126.2 W0.52 cal/h and M=82.6 W0.53 cal/h, respectively), as recorded in the literature. Zhe average for the African Crocidurinae is much lower (M= 43.6 W0.67). The metabolic rate of the European Croccidura russula agrees with that of the African species. Thus, the Crocidurinae are characterized by a relatively low metabolic rate; the Soricinae, and in particular the tribe of the Soricini, by an extremely high metabolic rate. The tribes Neomyini and Blarinini occupy an intermediate position. These differences are also to be found at the level of the basal metabolism. This main difference between the two sub-families can most likely be explained by evolution in geographical isolation under differential climatic conditions: the Crocidurinae having evolved in tropical Africa and the Soricinae in temperate Eurasia

White grubs (Coleoptera, Melolonthidae) in the "Planalto Region", Rio Grande do Sul state, Brazil: Key for identification, species richness and distribution

White grubs (Coleoptera, Melolonthidae) in the "Planalto Region", Rio Grande do Sul state, Brazil: Key for identification, species richness and distribution. The objective of this study was to survey the occurrence and geographic distribution of white grub species (Coleoptera, Melolonthidae) in cultivated and non-cultivated fields of the "Planalto Region", Rio Grande do Sul state, Brazil and develop a key at genus-level. Twenty-eight species from 15 genera and three subfamilies were recorded: Dynastinae, Melolonthinae and Rutelinae. The species or genera recorded for the first time in the state are: Cyclocephala metrica, C. tucumana, Isonychus albicinctus, Liogenys bidenticeps, L. fusca, L. obesa and L. sinuaticeps, Paranomala violacea, as well as unidentified species of Amononyx, Dicrania, Leucothyreus, Macrodactylus, Plectris and Rhizogeniates. Among the species recorded, 23 were associated with winter crops. Only Cyclocephala metrica, Dyscinetus rugifrons, two species of Leucothyreus and one species of the tribe Sericini were not present in cultivated crop fields. Cyclocephala flavipennis and Diloboderus abderus occurred in most of the municipalities sampled, often associated with Plectris sp., C. modesta and C. putrida. The highest richness of melolonthids was concentrated in the northeast of the Planalto region.

Observations on fragrance collection behaviour of euglossine bees (Hymenoptera, Apidae)

Male bees of the tribe Euglossini collect volatile chemicals secreted by orchids using dense patches of hair on the front tarsi. After collecting chemicals, the bee hovers while transferring these fragrances to invaginations on the hind tibiae. The fragrance collection and hovering behaviours are repeated multiple times. Here I report preliminary field observations on the length of fragrance collection and hovering phases in bees of the Eulaema meriana (Oliver, 1789) mimicry complex visiting the orchid Catasetum discolor in Kavanayén,Venezuela. I observed that in extended visits with many cycles of fragrance collection and hovering, the length of each collection phase gradually increased, while the length of hovering phase was static. This suggests either that chemicals secreted by orchids are in limited supply or that efficiency of fragrance collection drops.

Taxonomic review of Gallio Evans, 1955 (Lepidoptera, Hesperiidae, Hesperiinae): one less monotypic genus of Moncini

ABSTRACT Moncini is the tribe of Hesperiidae that comprises the greatest diversity of small, brown, hard to identify skippers. The group is peculiarly classified as having many monotypic genera, thus offering low informative value to its systematics. This study presents a review of the genus Gallio Evans, 1955, a genus formerly recognized as monotypic, and describes three new species, Gallio imperatriz sp. nov. from Maranhão, Brazil, Gallio furtadoi sp. nov. from Mato Grosso, Brazil and Gallio eti sp. nov. from Madre de Díos, Peru and Acre, Brazil (type locality). A lectotype for Vehilius carasta Schaus, 1902 is designated. Gallio is therefore redescribed and illustrations and diagnosis to its species are provided.

Putative adverse outcome pathways relevant to neurotoxicity.

The Adverse Outcome Pathway (AOP) framework provides a template that facilitates understanding of complex biological systems and the pathways of toxicity that result in adverse outcomes (AOs). The AOP starts with an molecular initiating event (MIE) in which a chemical interacts with a biological target(s), followed by a sequential series of KEs, which are cellular, anatomical, and/or functional changes in biological processes, that ultimately result in an AO manifest in individual organisms and populations. It has been developed as a tool for a knowledge-based safety assessment that relies on understanding mechanisms of toxicity, rather than simply observing its adverse outcome. A large number of cellular and molecular processes are known to be crucial to proper development and function of the central (CNS) and peripheral nervous systems (PNS). However, there are relatively few examples of well-documented pathways that include causally linked MIEs and KEs that result in adverse outcomes in the CNS or PNS. As a first step in applying the AOP framework to adverse health outcomes associated with exposure to exogenous neurotoxic substances, the EU Reference Laboratory for Alternatives to Animal Testing (EURL ECVAM) organized a workshop (March 2013, Ispra, Italy) to identify potential AOPs relevant to neurotoxic and developmental neurotoxic outcomes. Although the AOPs outlined during the workshop are not fully described, they could serve as a basis for further, more detailed AOP development and evaluation that could be useful to support human health risk assessment in a variety of ways.

Implementing 2008 Iowa Acts, Chapter 1187, Section 5 Tribal TANF Matching Fund Formula Recommendation, December 29, 2008

The Iowa Department of Human Services in consultation with the Winnebago Tribe of Nebraska submits the following recommendations as directed by 2008 Iowa Acts, Chapter 1187, Section 5.

Genotype-phenotype analysis of Jervell and Lange-Nielsen syndrome in six families from Saudi Arabia.

We sought to explore the genotype-phenotype of Jervell and Lange-Nielsen syndrome (JLNS) patients in Saudi Arabia. We have also assessed the plausible effect of consanguinity into the pathology of JLNS. Six families with at least one JLNS-affected member attended our clinic between 2011 and 2013. Retrospective and prospective clinical data were collected and genetic investigation was performed. Pathogenic mutations in the KCNQ1 gene were detected in all JLNS patients. The homozygous mutations detected were Leu273Phe, Asp202Asn, Ile567Thr, and c.1486_1487delCT and compound heterozygous mutations were c.820_ 830del and c.1251+1G>T. All living JLNS patients except one had a QTc of >500 ms and a history of recurrent syncope. β-Blockers abolished the cardiac-related events in all patients except two siblings with homozygous Ile567Thr mutation. Four of the six mutations were originally reported in autosomal dominant long QT syndrome (LQTS) patients. Eighty percent of the heterozygote mutation carriers showed prolongation of QTc, but majority of these reported no symptoms attributable to arrhythmias. Mutations detected in this study will be advantageous in tribe and region-specific cascade screening of LQTS in Saudi Arabia.

Reproductive morphology in Mediterranean Seirospora giraudyi (Ceramiales, Rhodophyta)

This paper presents a detailed description of the reproductive characters of Mediterranean Seirospora giraudyi based on fresh material collected in the northwestern coast of Spain. Vegetative cells are uninucleate. The plant is monoecious. Spermantangial parent’s cells are clustered on modified dwarf determinate filaments, usually situated on adaxial surfaces of branches. One to four spermatia are formed by elongation and proximal divisions of the spermatangial parent cells. Spermatium with a nucleus situated ina mec. The thallus is procarpic. The four-celled carpogonial branch is initially L-shaped, and it is situated on a periaxial supporting fertile axial cell. The mature carpogonial branch is U-shaped and the supporting cell and second periaxial cell enlarge and divide transversely to reproduce a pair uninicleate auxiliary cell. The nucleus in the ferlilized carpogonium divides twice and the carpogonium cleaves vertically into two cells that, turn, cut off a pair of uninucleate connecting cells that fuse with the auxiliary cells on opposite sides; the diploid nuclei in the connecting cells divide at the site of fusion and one of the nuclei enters the auxiliary cell white the other is extruded. Each auxiliary cell gives to a terminal primary gonimolobe initials. Gonimolobes form lax chains of carposporangia. As the gonimoblasts mature, both lobes of the foot cell which is situated on the supporting cell elongate the upper one secondary connecting with the supporting cell, and the lower one with the fertile axial cell. The gonimoblasts are subtended at maturity by one to several clusters of involucral flaments. Seirospora is currently placed in the tribe Euptiloteae; however the reproductive character of S.giraudyi is dentical to those described for the Cañllithamnieae. Molecular studies are needed to confirm the taxonomic position of S.giraudyi as well as that of the other species placed Seirospora

Back to Gondwanaland: can ancient vicariance explain (some) Indian Ocean disjunct plant distributions?

Oceans, or other wide expanses of inhospitable environment, interrupt present day distributions of many plant groups. Using molecular dating techniques, generally incorporating fossil evidence, we can estimate when such distributions originated. Numerous dating analyses have recently precipitated a paradigm shift in the general explanations for the phenomenon, away from older geological causes, such as continental drift, in favour of more recent, long-distance dispersal (LDD). For example, the 'Gondwanan vicariance' scenario has been dismissed in various studies of Indian Ocean disjunct distributions. We used the gentian tribe Exaceae to reassess this scenario using molecular dating with minimum (fossil), maximum (geological), secondary (from wider analyses) and hypothesis-driven age constraints. Our results indicate that ancient vicariance cannot be ruled out as an explanation for the early origins of Exaceae across Africa, Madagascar and the Indian subcontinent unless a strong assumption is made about the maximum age of Gentianales. However, both the Gondwanan scenario and the available evidence suggest that there were also several, more recent, intercontinental dispersals during the diversification of the group.

Present day distribution and historical biogeography of the tribu Ophidiini (Ophidiformes, Ophidiidae, Ophidiinae) from the East Tropical Atlantic (CLOFETA area) and North-East Atlantic and Mediterranean (CLOFNAM area)

The present day geographic distribution of the Ophidiini tribe (Ophidiidae, Ophidiinae) in the Clofnam (North- Eastern Atlantic and Mediterranean) and Clofeta (Eastern Tropical Atlantic) areas is revised in this paper. Results show that Parohidion vassali is not a Mediterranean endemic species, and the presence of Ophidion barbatum in the Atlantic is confirmed. Moreover, the paper tries to analyse the historical events which could have caused the present situation of two genera, Ophidion and Parophidion, both in the Atlantic and in the Mediterranean. Although first fossil records of Ophidion and Parophidion date from the Pliocene, when considering all the historical events occurred from the existence of the Tethys Sea to the opening of the Atlantic and the Mediterranean formation, a much earlier origin of these genera seems to be more likely. The situation of Ophidion barbatum and O. rochei in the Mediterranean and Black Sea is also discussed

Subfamilial and tribal relationships of Ranunculaceae: evidence from eight molecular markers

The first molecular phylogenies of the flowering plant family Ranunculaceae were published more than twenty years ago, and have led to major changes in the infrafamilial classification. However, the current phylogeny is not yet well supported, and relationships among subfamilies and tribes of Ranunculaceae remain an open question. Eight molecular markers from the three genomes (nuclear, chloroplast and mitochondrial) were selected to investigate these relationships, including new markers for the family (two homologs of the nuclear CYCLOIDEA gene, the chloroplast gene ndhF, and the mitochondrial intron nad4-I1). The combination of multiple markers led to better resolution and higher support of phylogenetic relationships among subfamilies of Ranunculaceae, and among tribes within subfamily Ranunculoideae. Our results challenge the monophyly of Ranunculoideae as currently circumscribed due to the position of tribe Adonideae (Ranunculoideae), sister to Thalictroideae. We suggest that Thalictroideae could be merged with Ranunculoideae in an enlarged single subfamily.

Elinkaariarvioinnin hyödyntäminen pumpputehtaan ympäristötoimenpiteiden tunnistamisessa

Voimistuvan kasvihuoneilmiön myötä kiinnostus hiilidioksidipäästöjen vähentämiseen on lisääntynyt. Ympäristöteknologian tavoitteena on hidastaa tämän ilmiön haitallisia vaikutuksia maailmanlaajuisesti, kansallisesti sekä yritystasolla. Yrityksillä on yhä suurempi paine valmistaa tuotteita ympäristöä vähemmän kuormittavasti. Työn tavoitteena on tarkastella elinkaariarvioinnin käytettävyyttä, kun halutaan tunnistaa pumpputehtaan mahdollisuuksia pienentää tuotteidensa valmistuksen ympäristönkuormittavuutta. Työssä pyritään myös selvittämään ne pumpun valmistuksen prosessit, jotka tuottavat eniten kasvihuonekaasupäästöjä. Tarkastelussa on keskitytty lähinnä hiilidioksidipäästöjen laskentaan. Työssä on käytetty elinkaariarvioinnin menetelmää ja työ noudattaa SFS ISO 14040 ja 14044 standardia. Elinkaariarviointi on hyväksi havaittu keino tunnistaa ne tuotteen tai palvelun yksikköprosessit, joilla on suurin vaikutus ympäristöön. Tutkimuksessa luotiin elinkaariarviointi pumppulaitoksen vuosittain tuottamille pumpuille. Samalla toteutettiin elinkaariarviointiin Excel-malli, jolla voidaan arvioida yksittäisten pumppujen valmistuksen hiilidioksidipäästöt. Tulosten pohjalta on tehty myös joitakin ehdotuksia pumpun valmistuksen hiilidioksidipäästöjen vähentämiseksi. Laskennassa käytetty aineisto kerättiin kirjallisuudesta, pumppulaitoksen alihankkijoilta sekä julkaistuista elinkaariarvioinneista vuoden 2008 kesän aikana. Työssä on osoitettu, että elinkaariarviointia voidaan hyödyntää tunnistettaessa pumpputehtaan ympäristötoimenpiteitä. Saatujen tulosten luotettavuus on kuitenkin riippuvainen käytettävissä olevista resursseista sekä lähtötietojen tarkkuudesta. Erityisesti valimoiden ilmoittamat energiankulutusmäärät vaikuttavat tulosten tarkkuuteen. Tarkempien tulosten saaminen edellyttäisi muun muassa tehtaan alihankkijoiden parempaa tietoisuutta omasta energiankulutuksestaan.

Ovule ontogenesis and megasporogenesis in Adesmia latifolia (Spreng.) Vog. (Leguminosae-Papilionoideae)

The ovule ontogenesis and the megasporogenesis events were studied under bright field, fluorescence and scanning electron microscopy. The primordium is 3-zonate and gives rise to a hemianatropous, bitegmic and crassinucellate ovule. The archesporium may consist of one or more archesporial cells, but only one undergoes meiosis, forming a linear tetrad. Normally, only a single megaspore is functional in the chalazal position, but occasionally two functional chalazal megaspores arise. The present study provides additional information on embryological characters in the Adesmieae tribe and discusses their taxonomic significance to the Leguminosae family.

Reproductive biology of the herkogamous vine Chiococca alba (L.) Hitchc. (Rubiaceae) in the Atlantic Rain Forest, SE Brazil

The family Rubiaceae comprises a wide spectrum of floral mechanisms and two of them seem to be common in certain groups, e.g., distyly in Rubioidae and styllar pollen in Ixoroidae. These mechanisms include herkogamy, which is interpreted as a strategy that avoids self-pollination. This is the first report on the reproductive biology of Chiococca alba, a species that is widely distributed in America. We studied floral biology and the mating system, which were evaluated through fruit set comparisons after controlled crosses (self- and cross-pollinations and test for apomixis), as well as through the evaluation of pollen tube growth resulting from these controlled crosses. Flowers of C. alba are herkogamous, cream, protandrous and lasted for two days. No measurable nectar was found, despite the presence of a nectary-like structure at the base of the corolla tube. Chiococca alba is a preferentially self-incompatible species, but self-pollination and apomixis also contribute to the natural fruit-set. Its reproductive strategy (herkogamy associated with protandry) is different from that expected for members of Chiococceae tribe (i.e., styllar pollen presentation).

Oil glands in the Neotropical genus Dahlstedtia Malme (Leguminosae, Papilionoideae, Millettieae)

Dahlstedtia pentaphylla (Taub.) Burkart and D. pinnata (Benth.) Malme belong to the Millettieae tribe and are tropical leguminous trees that produce a strong and unpleasant odour. In the present work, we investigated the distribution, development and histochemistry of foliar and floral secretory cavities that could potentially be related to this odour. The ultrastructure of foliar secretory cavities were also studied and compared with histochemical data. These data were compared with observations recorded for other species of Millettieae in order to gain a phylogenetic and taxonomic perspective. Foliar secretory cavities were only recorded for D. pentaphylla. Floral secretory cavities were present in the calyx, wings and keels in both species; in D. pinnata they also were found in bracteoles and vexillum. Such structures were found to originate through a schizogenous process. Epithelial cells revealed a large amount of flattened smooth endoplasmic reticula, well-developed dictyosomes and vacuoles containing myelin-like structures. Cavity lumen secretion stains strongly for lipids. Features of the secretory cavities studied through ultrastructural and histochemical procedures identify these structures as oil glands. Thus, if the odour produced by such plants has any connection with the accumulation of rotenone, as other species belonging to the "timbó" complex, the lipophilic contents of the secretory cavities of Dahlstedtia species take no part in such odour production. The presence, distribution patterns and frequencies of secretory structures in Dahlstedtia are taxonomically significant and may be utilized as a diagnostic character which justifies the separation of this genus into two species.