Effects of nitrogen limitation on Dunaliella sp.–Alteromonas sp. interactions: from mutualistic to competitive relationships.

Interactions between photosynthetic and non-photosynthetic microorganisms play an essential role in natural aquatic environments and the contribution of bacteria and microalgae to the nitrogen cycle can lead to both competitive and mutualistic relationships. Nitrogen is considered to be, with phosphorus and iron, one of the main limiting nutrients for primary production in the oceans and its availability experiences large temporal and geographical variations. For these reasons, it is important to understand how competitive and mutualistic interactions between photosynthetic and heterotrophic microorganisms are impacted by nitrogen limitation. In a previous study performed in batch cultures, the addition of a selected bacterial strain of Alteromonas sp. resulted in a final biomass increase in the green alga Dunaliella sp. as a result of higher nitrogen incorporation into the algal cells. The present work focuses on testing the potential of the same microalgae–bacteria association and nitrogen interactions in chemostats limited by nitrogen. Axenic and mixed cultures were compared at two dilution rates to evaluate the impact of nitrogen limitation on interactions. The addition of bacteria resulted in increased cell size in the microalgae, as well as decreased carbon incorporation, which was exacerbated by high nitrogen limitation. Biochemical analyses for the different components including microalgae, bacteria, non-living particulate matter, and dissolved organic matter, suggested that bacteria uptake carbon from carbon-rich particulate matter released by microalgae. Dissolved organic nitrogen released by microalgae was apparently not taken up by bacteria, which casts doubt on the remineralization of dissolved organic nitrogen by Alteromonas sp. in chemostats. Dunaliella sp. obtained ammonium-nitrogen more efficiently under lower nitrogen limitation. Overall, we revealed competition between microalgae and bacteria for ammonium when this was in continuous but limited supply. Competition for mineral nitrogen increased with nitrogen limitation. From our study we suggest that competitive or mutualistic relationships between microalgae and bacteria largely depend on the ecophysiological status of the two microorganisms. The outcome of microalgae–bacteria interactions in natural and artificial ecosystems largely depends on environmental factors. Our results indicate the need to improve understanding of the interaction/s between these microbial players

The 17th Annual Eric E. Williams Memorial Lecture

This flyer promotes an event for The 17th Annual Eric E.Williams Memorial Lecture, featuring the Honorable Dr. Ralph Everard Gonsalves, Prime Minister of Saint Vincent and the Grenadines. The event was held on October 23, 2015.

The 17th Annual Eric E. Williams Memorial Lecture- The US-Cuba Accord: How the Caribbean Paved the Way

This flyer promotes an event for The 17th Annual Eric E.Williams Memorial Lecture, featuring the Honorable Dr. Ralph Everard Gonsalves, Prime Minister of Saint Vincent and the Grenadines. The event was held on October 23, 2015 at the Wertheim Performing Arts Center, Florida International University.

Che cosa significa emancipazione? Organizzazione sociale, norme giuridiche e potere paterno dalla Rivoluzione francese agli anni Quaranta dell'Ottocento

La tesi presenta un’analisi storico-concettuale del concetto di emancipazione in Francia tra la Rivoluzione francese e gli anni Quaranta dell’Ottocento a partire dalla centralità delle donne nel processo rivoluzionario e dal problema del potere paterno e dell’ordine della famiglia. Da questa prospettiva viene ricostruito il momento di affermazione del significato moderno del concetto: prima con l’Illuminismo e poi con la Rivoluzione francese si determina un progressivo allargamento semantico che farà posto a significati politico-sociali inediti e a una serie di soggetti imprevisti. La Rivoluzione presenta infatti sulla scena un soggetto collettivo, già maturo, che dà forma all’emancipazione rivoluzionaria. Quest’ultima richiede una specifica scienza in grado di arginare i suoi effetti politici più pericolosi senza tradirne le premesse formali. A questo risponde l’Idéologie come scienza sociale sul finire della Rivoluzione. Se le donne in questi anni si mobilitano a partire dall’emancipazione rivoluzionaria, esse rappresentano al tempo stesso un problema fondamentale per l’ordine rivoluzionario come mostra il dibattito sul potere paterno che fa luce sul passaggio, fondamentale per il concetto di emancipazione, dal potere di emancipare del padre a quello dello Stato emancipatore. Gli esiti di questo dibattito si trovano nel Codice civile e nella sua emancipazione codificata, che definisce i confini giuridici e formali dell’emancipazione rivoluzionaria e le sue gerarchie. In seguito, la tesi ricostruisce le trasformazioni del concetto all’indomani della Rivoluzione nella dottrina di Saint-Simon e dei suoi allievi. Questa sarà all’origine del discorso che si sviluppa in Francia tra gli anni Trenta e Quaranta sul problema dell’emancipazione della donna che trova espressione negli scritti delle donne saint-simoniane. La tesi si ferma alle porte degli anni Quaranta quando l’emancipazione nel suo significato moderno si stabilizza e diventa un vero e proprio concetto di movimento, espressione di processi di emancipazione che si erano preparati negli anni precedenti.

Collaborative governance of smart cities: addressing multiple relations of actors involved

This dissertation aims to contribute to the discourse on the governance of smart cities (SC) by examining the collaborative relationships between various actors involved in developing and implementing SC initiatives. Poorly organized collaboration can lead to conflicts and misunderstandings, resulting in failures in realizing such complex technological initiatives. Hence, capturing the main elements of SC collaboration becomes essential for understanding how they should be developed and managed. However, the topic has been limitedly explored in prior research, with fragmented studies on narrow aspects related to the SC governance. Using Russia as an empirical setting, the study focuses on the interplay of both government and non-governmental stakeholders in constructing collaborative relationships within SC, covering both vertical and horizontal dimensions of their interaction. The overarching goal of this research is to understand how collaborative governance unfolds in the SC context by stating two guiding research questions: 1) who are the dominant actors in SC and what are their roles? 2) what are the relationships forged among them? The dissertation investigates the SC initiatives across three different cities – Moscow, Saint Petersburg, and Perm – in a format of empirical illustration as well as an in-depth case study. The dissertation provides three main contributions. First, it strengthens the link between the SC domain, public governance, and literature on cross-sectoral collaboration by highlighting ‘urban smartness’ as a source for generating multiple values. Second, the thesis offers novel view on the strategic development paths which conceptually shape the SC framework. It connects the techno-centric and human-centric perspectives of SC by showing that they are naturally linked, rather than mutually exclusive. Third, the study illustrates that SC initiatives are contextually dependent, and this dependence covers specificities of public governance, including underlying informal mechanisms, which influence the inception, development, and management of SC in the organizational realms.

Report on a familiarization tour of Montserrat, St. Kitts-Nevis-Anguilla, Antigua, St. Lucia and Barbados (8-25 March 1977)

Informe sobre el desarrollo industrial en Montserrat, San Cristóbal Nieves y Anguila, Santa Lucía y Barbados. Analiza brevemente algunas industrias específicas e identifica proyectos industriales que podrían desarrollarse en estos países

A profile of small farmers in the Caribbean region : Antigua, Barbados, Belize, British Virgin Is., Cayman Is., Dominica, Grenada, Montserrat, St. Kitts-Nevis-(Anguilla), St. Lucia, St. Vincent, Turks & Caicos Is. /

Bibliography: p. [99]-101.

Morphological and morphometric appraisal of the spermatophore of the southern hermit crab Isocheles sawayai Forest and Saint Laurent, 1968 (Anomura: Diogenidae), with comments on gonopores in both sexes

The spermatophore morphology of the hermit crab Isocheles sawayai from Southwestern Atlantic (Brazil) is described. The spermatophores show similarities with those described for other members of the family Diogenidae, especially with the recently described Loxopagurus loxochelis. The spermatophore is composed of three major regions: a sperm filled head or ampulla, a columnar stalk and a foot or pedestal. The spermatophores show specific morphology in having a circular ampulla, and a constriction or neck between the ampulla (100 mu m) and the thin (27 mu m), long stalk (500 mu m). The stalk penetrates less than half way into the spermatophore head. Most spermatophores show one or the small posterior projections on the underside of the ampulla as being bigger than the other, making it asymmetrical. The size of the spermatophore is related to hermit crab size with direct relationships found between spermatophore ampulla width, total length, and peduncle length with shield length of the hermit crab. The morphological characteristics of the spermatophore of L sawayai. are species-specific distinguishing it from other members of the family, and are useful to infer further phylogenetic relationships. (C) 2008 Elsevier GmbH. All rights reserved.

Serological evidence for Saint Louis encephalitis virus in free-ranging New World monkeys and horses within the upper Paraná River basin region, Southern Brazil

Introduction Saint Louis encephalitis virus (SLEV) primarily occurs in the Americas and produces disease predominantly in humans. This study investigated the serological presence of SLEV in nonhuman primates and horses from southern Brazil. Methods From June 2004 to December 2005, sera from 133 monkeys (Alouatta caraya, n=43; Sapajus nigritus, n=64; Sapajus cay, n=26) trap-captured at the Paraná River basin region and 23 blood samples from farm horses were obtained and used for the serological detection of a panel of 19 arboviruses. All samples were analyzed in a hemagglutination inhibition (HI) assay; positive monkey samples were confirmed in a mouse neutralization test (MNT). Additionally, all blood samples were inoculated into C6/36 cell culture for viral isolation. Results Positive seroreactivity was only observed for SLEV. A prevalence of SLEV antibodies in sera was detected in Alouatta caraya (11.6%; 5/43), Sapajus nigritus (12.5%; 8/64), and S. cay (30.8%; 8/26) monkeys with the HI assay. Of the monkeys, 2.3% (1/42) of A. caraya, 6.3% 94/64) of S. nigritus, and 15.4% (4/26) of S. cay were positive for SLEV in the MNT. Additionally, SLEV antibodies were detected by HI in 39.1% (9/23) of the horses evaluated in this study. Arboviruses were not isolated from any blood sample. Conclusions These results confirmed the presence of SLEV in nonhuman primates and horses from southern Brazil. These findings most likely represent the first detection of this virus in nonhuman primates beyond the Amazon region. The detection of SLEV in animals within a geographical region distant from the Amazon basin suggests that there may be widespread and undiagnosed dissemination of this disease in Brazil.

A Saint Louis encephalitis and Rocio virus serosurvey in Brazilian horses

Introduction Arboviruses are an important public health problem in Brazil, in especially flaviviruses, including the Saint Louis encephalitis virus (SLEV) and the Rocio virus (ROCV), are especially problematic. These viruses are transmitted to humans or other vertebrates through arthropod bites and may cause diseases with clinical manifestations that range from asymptomatic infection, viral hemorrhagic fever to encephalitis. Methods A serological survey of horses from various regions of Brazil using an enzyme-linked immunosorbent assay (ELISA) with recombinant SLEV domain III peptides and ROCV E protein as antigens. Results Overall, 415 (55.1%) of the 753 horses that were screened were seropositive for flavivirus and, among them, monotypic reactions were observed to SLEV in 93 (12.3%) and to ROCV in 46 (6.1%). These results suggested that these viruses, or other closely related viruses, are infecting horses in Brazil. However, none of the studied horses presented central nervous system infection symptoms. Conclusions Our results suggest that SLEV and ROCV previously circulated among horses in northeast, west-central and southeast Brazil.

Role of Intersectoral Collaboration in Mental Health Policy Formulation And Service Delivery in Saint Lucia

RESUMO: Santa Lúcia pequena ilha de país em desenvolvimento com recursos limitados e é confrontada com uma série de desafios socioeconômicos que exigem soluções criativas e inovadoras. É comprovado que a combinação de recursos entre setores para estabelecer os determinantes social, econômico e ambiental da saúde são uma estratégia útil para melhorar a saúde da população, principalmente a sua saúde mental. Este estudo, o primeiro do seu tipo em Santa Lúcia, procurou examinar até que ponto a disponibilidade de uma política nacional de saúde mental levou a ação intersetorial para o fornecimento de serviços e promoção da saúde mental. Além disso, o estudo examinou o nível de colaboração intersetorial que existe entre as agências que prestam cuidados diretos e serviços de suporte para pessoas com doenças mentais e problemas sérios de saúde mental. O estudo também teve como objetivo identificar os fatores que promovem ou dificultam a colaboração intersectorial e gerar recomendações que possam ser aplicadas para países muito pequenos e com perfis socioeconômicos semelhantes. Os dados gerados a partir de três (3) fontes foram sintetizados para formar uma visão ampla das questões. Uma avaliação da política de saúde mental de 2007, uma avaliação que identifica até que ponto a ação intersetorial atualmente deixa a prestação de serviços de saúde mental e a administração de entrevistas semiestruturadas nas mãos de gestores do programa de diferentes agências em todos os setores. O estudo concluiu que, apesar da disponibilidade de uma política de saúde mental, que articula clara e explicitamente a colaboração intersetorial como área prioritária para ação, quase não existe no sistema de fornecimento atual do serviço. Os provedores de serviços em todos os setores reconhecem que há os benefícios da colaboração intersectorial e com entraves significativos em relação à colaboração intersetorial, que por sua vez, impede uma abordagem nacional para o planejamento e o fornecimento do serviço. A colaboração intersetorial não será possível se os próprios setores dependerem da abordagem direta do setor da saúde ou se a atmosfera geral for ofuscada pela estigmatização das doenças mentais.------------------------------------------------------------------------ABSTRACT: Saint Lucia a small island developing country with limited resources, is faced with a number of socio-economic challenges which require creative and innovative solutions to address. Combining resources across sectors to address the social, economic and environmental determinants of health has proven to be a useful strategy for improving population health in particular mental health. This study, the first of its kind for Saint Lucia sought to examine the extent to which the availability of a national mental health policy led to intersectoral action for mental health promotion and service delivery. In addition the study examined the level of intersectoral collaboration which actually exist between agencies which provide direct care and support services to people with mental illnesses and significant mental health problems. The study also aimed to identify the factors which promote or hinder intersectoral collaboration and generate recommendations which can be applied to extremely small countries with similar socio-economic profiles. Data generated from three (3) sources was synthesized to form a broad picture of the issues. An evaluation of the mental health policy of 2007, an assessment of the extent to which intersectoral action currently exist in mental health service delivery and the administration of semi-structured interviews with program managers from different agencies across sectors to identify implementation issues. The study concluded that despite the availability of a mental health policy which clearly and explicitly articulates intersectoral collaboration as a priority area for action, very little exists in the current service delivery system. Services providers across sectors acknowledge the benefits of intersectoral collaboration and that there are significant barriers to intersectoral collaboration, which in turn hinders a national approach to service planning and delivery. Intersectoral collaboration is not possible if sectors themselves are dependent on a top-down health sector driven and dominated approach, or if the general atmosphere is clouded by stigmatization of mental health illnesses.

Serologic evidence of the recent circulation of Saint Louis encephalitis virus and high prevalence of equine encephalitis viruses in horses in the Nhecolândia sub-region in South Pantanal, Central-West Brazil

As in humans, sub-clinical infection by arboviruses in domestic animals is common; however, its detection only occurs during epizootics and the silent circulation of some arboviruses may remain undetected. The objective of the present paper was to assess the current circulation of arboviruses in the Nhecolândia sub-region of South Pantanal, Brazil. Sera from a total of 135 horses, of which 75 were immunized with bivalent vaccine composed of inactive Eastern equine encephalitis virus (EEEV) and Western equine encephalitis virus(WEEV) and 60 were unvaccinated, were submitted to thorough viral isolation, reverse transcriptase polymerase chain reaction (RT-PCR) and neutralization tests for Saint Louis encephalitis virus (SLEV), EEEV, WEEV and Mayaro virus (MAYV). No virus was isolated and viral nucleic-acid detection by RT-PCR was also negative. Nevertheless, the prevalence of neutralizing antibodies in horses older than seven months was 43.7% for SLEV in equines regardless of vaccine status, and 36.4% for WEEV and 47.7% for EEEV in unvaccinated horses. There was no evidence of MAYV infections. The serologic evidence of circulation of arboviruses responsible for equine and human encephalitis, without recent official reports of clinical infections in the area, suggests that the Nhecolândia sub-region in South Pantanal is an important area for detection of silent activity of arboviruses in Brazil.