Padronização e aplicação da técnica de ELISA (Enzyme-linked immunosorbent assay) indireto com anticorpo de captura para a detecção de anticoepos contra o cicovírus suíno tipo 2

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Avaliação imunofenotípica de subpopulações linfocitárias no sangue do cordão umbilical e periférico de suínos neonatos (Sus scrofa)

Considering the importance of umbilical cord blood as a potential source of stem cell and, on the other hand, the use of the domestic swine (Sus scrofa) as a useful model for biomedical research in regenerative medicine and aiming to contribute about the quantification of lymphocyte subsets in umbilical cord blood and peripheral blood of newborn piglets, this study aimed to quantify CD4+, CD5+ and CD8+ cells from umbilical cord blood and peripheral blood from pigs at term blood samples. Were analyzed samples of the umbilical cord blood and peripheral of 48 piglets of Topigs lineage, from healthy mothers, artificially inseminated and natural birth. Blood samples were collected from the umbilical cord at birth, by the umbilical vein, and peripheral blood by venous sinus retro-ophthalmic. The immunological measurements of CD4+, CD5+ and CD8+ were obtained by flow cytometry. The relative average values for the CD4+, CD5+ e CD8+ counts in umbilical cord blood and peripheral blood of newborn piglets were inferior to those reported for peripheral blood in adult pigs, suggesting immunological immaturity. The ratio CD4+:CD8+ in umbilical cord blood (3.2±1.2%) and peripheral blood (3.2±1.7%) showed a predominance of TCD4+ over TCD8+. The percentage of CD4+ and CD8+ cells was 1.37±0.86% and 1.15±0.57%, respectively, in umbilical cord blood and peripheral blood.

Obtencao de animais negativos para Circovirus suino 2 oriundos de granjas positivas: estrategia de manejo

O objetivo do trabalho é descrever uma estratégia para a obtenção de animais negativos para o PCV2 oriundos de uma granja positiva para este vírus. Dezesseis leitões foram obtidos de fêmeas que tiveram os títulos de IgG anti-PCV2 e o DNA viral testados durante a gestação. Esses leitões, aos sete e dez dias de idade, foram transferidos para a unidade de pesquisa. Durante o período de 7 e 10 aos 49 e 52 dias de idade, amostras de soro, suabes nasal e fecal foram coletadas, a cada sete dias. Após esse período, três animais permaneceram na unidade de pesquisa e foram acompanhados dos 49 aos 114 dias de idade, com coletas realizadas a cada 28 dias. Não houve diferença significativa (p = 0,317) de viremia entre marrãs (n = 6) e porcas (n = 10). Com relação aos níveis de IgG, observou-se diferença significativa (p = 0,0213) entre porcas e marrãs. Os leitões (n = 16), obtidos de duas fêmeas, foram transferidos para a unidade de pesquisa. Os animais entre 7 e 10 dias e aos 49 e 52 dias de idade apresentaram queda de IgG e ausência de IgM anti-PCV2; e as amostras de soro, suabe nasal e fecal foram negativos para o DNA de PCV2. Após os 49 dias, nos três animais mantidos isolados, a detecção de IgG, IgM e DNA para PCV2 permaneceu negativa. Concluindo, a estratégia de manejo utilizada permitiu obter suínos negativos para PCV2 oriundo de granjas positivas para o agente.

Perfil tecnológico e funcional de cepas probióticas em leite fermentado

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Diferenciação do leite convencional e orgânico, por análises de isótopos estáveis e de compostos antioxidantes

Pós-graduação em Zootecnia - FMVZ

Comportamento de Escherichia coli produtora de toxina de Shiga em meios ácidos

Escherichia coli é uma bactéria Gram-negativa, pertencente à família Enterobacteriaceae, que habita o intestino de animais de sangue quente, com 90% sendo comensais para o ser humano e 10% patogênicas. Quando presente em alimentos esse grupo é considerado um bom indicador higiênico-sanitário de alimentos. Em alimentos, a cepa patogênica mais estudada é E. coli produtora de toxina de Shiga (STEC) O157:H7. Entretanto, muitos casos vêm ocorrendo em todo o mundo devido a cepas patogênicas não O157, como O103, O111, O145 e O26. As STECs são responsáveis por desde uma simples diarréia até diarréia sanguinolenta que pode evoluir ainda para síndrome hemolítica urêmica e púrpura trombótica trombocitopênica, que podem ocasionar danos crônicos como falência renal. A transmissão destas cepas se deve a carnes mal cozidas, leites e derivados não pasteurizados, água e vegetais contaminados. Uma característica interessante de E. coli O157:H7 é o seu mecanismo de resistência ao estresse ácido – ácido tolerância – de modo que os alimentos ácidos não podem mais ser considerados seguros contra tais organismos. A fim de ampliar o conhecimento acerca das cepas não O157 e dos mecanismos de ácido resistência, este trabalho teve por objetivo verificar o comportamento de linhagens de STEC não O157:H7 submetidas às condições de estresse de baixo pH utilizando para tanto meios de cultura acidificados e polpas de frutas como matriz alimentar. Constatou-se que quando mantidas em condições-controle e as ácido-adaptadas mantêm populações da ordem de 2 a 3 log UFC/mL quando em polpas de frutas sabor cajá (pH 2,3) e tamarindo (pH 2,3) armazenadas a 4 C por até 30 dias

Leuconostoc mesenteroides SJRP55 isolada de mussarela de búfala: perfil probiótico e bacteriocinogênico

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Modelos para estimar exigências nutricionais de aminoácidos e resposta à ingestão de metionina: sistema tradicional por fases x nutrição de precisão

Pós-graduação em Zootecnia - FCAV

Inorganic and organic trace mineral supplementation in weanling pig diets

A study was conducted to evaluate the s of dietary inorganic and organic trace minerals in two levels of supplementation regarding performance, diarrhea occurrence, hematological parameters, fecal mineral excretion and mineral retention in metacarpals and liver of weanling pigs. Seventy piglets weaned at 21 days of age with an average initial body weight of 6.70 ± 0.38 kg were allotted in five treatments: control diet (no added trace mineral premix); 50% ITMP (control diet with inorganic trace mineral premix supplying only 50% of trace mineral requirements); 50% OTMP (control diet with organic trace mineral premix supplying only 50% of trace mineral requirements); 100% ITMP (control diet with inorganic trace mineral premix supplying 100% of trace mineral requirements); and 100% OTMP (control diet with organic trace mineral premix supplying 100% of trace mineral requirements). Feed intake and daily weight gain were not affected by treatments, however, piglets supplemented by trace minerals presented better gain:feed ratio. No differences were observed at calcium, phosphorus, potassium, magnesium, sodium and sulfur excreted in feces per kilogram of feed intake. Treatments did not affect calcium, phosphorus, magnesium, sulfur and iron content in metacarpals. Trace mineral supplementation, regardless of level and source, improved the performance of piglets.

Relative bioavailability of iron from organic sources for weanling piglets

Some authors consider minerals from organic sources more bioavailable for pig nutrition in comparison with inorganic sources. To evaluate the relative iron bioavailability from the organic source iron carbo-amino-phospho-chelate (ICAPC) to weanling piglets, it was conducted an experiment with 126 commercial piglets, using iron sulfate monohydrate (S) as standard. The experiment had a randomized block design with seven treatments (diet without adding specific source of iron, diet with 50, 100 and 150 ppm iron from S and diet with 50, 100 and 150 ppm iron from ICAPC), six replications and three animals per experimental unit. Performance parameters (average daily gain - ADG, feed: gain ratio - F:G) and blood variables (hemoglobin - Hb, hematocrit - Ht, transferrin - TR, latent iron-binding capacity - LIBC, total iron-binding capacity - TIBC, serum iron - Fe and transferrin saturation index - TSI) were evaluated. At the end of the experiment a piglet from each experimental unit was slaughtered and its liver and spleen removed for assessment of iron concentration by flame atomic absorption spectrometry (FAAS). The evaluated sources of iron yielded similar results for the variables of interest, but the increase in iron intake was followed by a linear increase in ADG, Hb, Ht, Fe and TSI as well as a linear decrease in the values of F:G, TR, LIBC and TIBC. Iron bioavailabilities from both ICAPC and S sources are similar for weanling piglets.

Epidemiologia e prevalência de infecções pelo Vírus da Diarreia Viral Bovina (BVDV) em suínos de criações não tecnificadas

Pós-graduação em Medicina Veterinária - FCAV

Ácidos orgânicos em dietas de suínos

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Parenteral administration of vitamins A, D and E on the oxidative metabolism and function of polymorphonuclear leukocytes in swine

The weaning period of piglets is characterized by physiological alterations, such as decreased weight gain, increased reactive oxygen species (ROS) and increased serum cortisol levels with possible effects on the immune response. The effect of parenteral administration of vitamins A, D and E on production performance, oxidative metabolism, and the function of polymorphonuclear leukocytes (PMNLs) was assessed in piglets during the weaning period. The sample was comprised of 20 male piglets that were given an injectable ADE vitamin combination (135,000 IU vitamin A, 40,000 IU vitamin D and 40mg vitamin E/animal) at 20 and 40 days of age. Weight gain, concentration of reduced glutathione (GSH), malondialdehyde (MDA), superoxide dismutase (SOD) and the microbicidal and phagocytic activity of PMNLs were assessed. No difference was observed in the average piglet weight during the study; however, a greater percentage of weight gain was observed after weaning in the treated group. The concentrations of GSH and SOD did not differ between groups, although lipid peroxidation was greater in the control group at 60 days of age. The investigated variables of oxidative metabolism were correlated as follows: -0.41 for GSH and MDA, -0.54 for GSH and SOD and 0.34 for MDA and SOD. The intensity of intracellular ROS production, the percentage of ROS-producing PMNLs and the intensity of phagocytosis by PMNLs did not differ between treatment groups. Administration of the injectable ADE combination improved the percentage of weight gain between 20 and 40 days of age, decreased oxidative stress at 60 days of age and did not influence the function of PMNLs in piglets.

Na 1 Nachlass Max Horkheimer, 32 - Korrespondenzen mit Familie Lehmann (p. I 16, 236-480)

6 Briefe zwischen E. Lederer und Max Horkheimer, 1936-1939; 1 Brief von Theodor W. Adorno an Minna Ledermann, 26.04.1941; 1 Brief von W. W. Lee an Max Horkheimer, 15.11.1938; 69 Brief zwischen Berta Lehmann, Flora Lehmann an Max Horkheimer, 1939-1944; 2 Briefe von Berta Lehmann, Flora Lehmann an Juliette Favez, März 1939; 1 Brief vom Reisebüro Anselm Stuttgart an Max Horkheimer, 02.04.1941; 4 Briefe zwischen der Auswandererstelle Marx Stuttgart und Max Horkheimer, 28.11.1940, 1941; 1 Brief von Max Horkheimer an Karl Adler, 24.01.1941; 2 Briefe von Max Horkheimer an Walter C. Louchheim, 1940; 5 Briefe zwischen dem American Consul General Stuttgart und Max Horkheimer, 1939-23.11.1940; 4 Brief zwischen der Auswandererstelle Adler Stuttgart und Max Horkheimer, 1940; 2 Briefe zwischen der Auswandererstelle Stuttgart und Max Horkheimer, 20.02.1940; 5 Briefe zwischen N. C. Leites und Max Horkheimer, 17.05.1937, 1937; 4 Briefe zwischen Irmgard Lenel und Max Horkheimer, 1941, 1942; 3 Briefe zwischen Heidi Lenssen und Max Horkheimer, 01.02.1937, 1937; 3 Briefe zwischen Theo F. Lentz und Max Horkheimer, 05.07.1945, 1945; 11 Briefe zwischen Jella Lepman und Max Horkheimer,1939-1941; 2 Briefe von Max Horkheimer an das American Consul General London, 1941; 1 Brief von R. Leppla an Max Horkheimer, 21.06.1948; 7 Briefe zwischen Max Lerner und Max Horkheimer, 1941, 1942; 5 Briefe und Beilagen zwischen Adolf Laschnitzer und Max Horkheimer, 1939-1940; 2 Briefe ziwschen dem The Emergency Committee in Aid of Displaced Foreign Scholars, New York und Max Horkheimer, 23.110.1940, 07.11.1940; 3 Briefe und 1 Beilage zwischen Andrée Lespiaut und Max Horkheimer, 13.11.1948; 1 Brief von Max Horkheimer an A. Lesser, 21.05.1935; 1 Brief von Bobby Level und Frank Level an Max Horkheimer, 20.07.1949; 1 Brief von Julius Walter Levi an Max Horkheimer, 15.05.1940; 1 Brief von Bernhard W. Levmore an Leo Löwenthal, 13.08.1940; 3 Briefe von Margot von Mendelssohn an Bernhard W. Levmore, 1940; 3 Briefe zwischen Ernst Levy und Max Horkheimer, 21.05.1927, 1937; 1 Brief von Erwin Levy an Max Horkheimer, 23.03.1935; 17 Briefe zwsichen Hanna Levy und Max Horkheimer, Friedrich Pollock, 1936-1937; 6 Briefe zwischen Herbert Levy und Friedichpollock, 1939-1940; 1 Brief von Friedrich Pollock an Heinz Langerhans, 11.08.1939; 2 Briefe von Max Horkheimer an die Society of the Protection of Science and Learning, The Scott Polar Research Institut, Cambridge, England, 24.11.1939; 2 Briefe zwischen Marie Levy und Max Horkheimer, 18.12.1938, 03.08.1939; 10 Briefe und 1 Beilage zwischen Max Lexandrowitz, Magarete Lexandrowitz und Max Horkheimer, 1940; 1 Brief vom National Refugee Service New York an Max Horkheimer, 19.03.1940; 1 Rechnung vom Librairie Generale de Droit & de Jurisprudence Paris an Max Horkheimer, 18.05.1938; 2 Briefe zwsichen L. Lichtwitz und Max Horkheimer, 16.04.1936, 25.07.1938;

Na 1 Nachlass Max Horkheimer, 642 - "Materialien. Druckseiten aus Zeitschriften", "Menschen im Großbetrieb" und "Vorwort zu: 'Zeugnisse. Theodor W. Adorno zum 60. Geburtstag'" (p. IX 20.11-76-IX 22)

11. Salomon, Albert: "The Spirit of the Soldier and Nazi Militarism". Social Research, Februar 1942, 13 Blatt; 12. Dicks, H.V.: "The Psychological Foundations of the Wehrmacht". Als Typoskript vervielfältigt, 42 Blatt; 13. Clark, Robert A.: "Aggressivness and Military Training". American Journal of Sociology, Volume 51, Number 5, March 1946, 5 Blatt; 14. Stagner, Ross: "Fascist Attitudes: Their Determening Conditions". The Journal of Social Psychology, Volume III, Number 4, 1936, 9 Blatt; 15. Apple, Kenneth E.: "Nationalism and Souvereignity: A Psychiatric View." The Journal of Normal and Abnorma Psychology, Volume 40, Number 4, October 1945, 4 Blatt; 16. Schreier, Fritz: "German Aggressivness- Its Reasons and Types". Journal of Normal and Abnormal Psychology, Volume 38, Number 2, April 1943, 7 Blatt; 17. Stagner, Ross: "Fascist Attitudes: An Exploratory Study". The Journal of Social Psychology, Volume III, Number 3, 1936, 6 Blatt; 18. Stagner, Ross und Katzoff, E. T.: "Fascist Attitudes: Factor Analysis of Item Correlations". The Journal of Social Psychology, 16, 1942, 4 Blatt; 19. Stagner, Ross und Osgood, Charles E.: "Impact of War on a Nationalistic Frame of Reference". The Journal of Social Psychology, 24, 1946, 15 Blatt; 20. Day, Daniel Droba und Quackenbusch, O.F.: Attitudes Towards Defensive, Cooperative and Aggressive War". The Journal of Social Psychology, 16, 1942, 5 Blatt; 21. Kecskemeti, Paul und Leites, Nathan: "Some Psychological Hypotheses on Nazi Germany: I". The Journal of Social Psychology, 26, 1947, 22 Blatt; 22. Dieselben: "Some Psychological Hypotheses on Nazi Germany: II". Ebenda, 27, 1948, 14 Blatt; 23. Parsons, Tollcott: "Certain Primary Sources and Pattersens of Aggression in the Social Structure of the Western World". Psychiatry, Volume 10, Number 2, May 1047, 8 Blatt; 24. Zerner, Elizabeth H.: "German Occupation and Anti-Semitism in France". Public Opinion Quarterly, Summer 1948, 5 Blatt; 25. Hauser, Ernest O.: "Doctor [Julian] Huxley`s Wonderful Zoo". The Saturday Evening Post, ohne Datum, 5 Blatt; 26. Zeitungsabschnitt, 1 Blatt; "Menschen im Großbetrieb" (GS 8, S. 95-105); Veröffentlicht in: Deutsche Zeitung, 19.02.1955. a) Typoskript mit dem Titel "Meinungsforschung im Betrieb" mit handschriftlichen Korrekturen, 10 Blatt b) Typoskript mit dem Titel "Der Mensch im Großbetrieb", mit eigenhändigen Korrekturen und einer handschriftlichen Notiz von Theodor W. Adorno, 17 Blatt c) Typoskript mit eigenhändigen Korrekturen, 17 Blatt d) Zeitungsdruck mit dem Titel "Menschen im Großbetrieb", mit eigenhändigen Korrekturen, 1 Blatt e)-f) Dasselbe , 1 Blatt; "Vorwort" zu: "Zeugnisse. Theodor W. Adorno zum 60. Geburtstag"; Veröffentlicht: Ebenda, Frankfurt am Main, 1963. a)-b) Typoskript mit eigenhändigen Korrekturen, 1 Blatt c) Typoskript, 2 Blatt;