Induction of the gene encoding mucosal vascular addressin cell adhesion molecule 1 by tumor necrosis factor alpha is mediated by NF-kappa B proteins.

Mucosal vascular addressin cell adhesion molecule 1 (MAdCAM-1) is involved in trafficking of lymphocytes to mucosal endothelium. Expression of MAdCAM-1 is induced in the murine endothelial cell line bEnd.3 by tumor necrosis factor alpha (TNF-alpha), interleukin 1, and bacterial lipopolysaccharide. Here we show that TNF-alpha enhances expression of a firefly luciferase reporter directed by the MAdCAM-1 promoter, confirming transcriptional regulation of MAdCAM-1. Mutational analysis of the promoter indicates that a DNA fragment extending from nt -132 to nt +6 of the gene is sufficient for TNF-alpha inducibility. Two regulatory sites critical for TNF-alpha induction were identified in this region. DNA-binding experiments demonstrate that NF-kappa B proteins from nuclear extracts of TNF-alpha-stimulated bEnd.3 cells bind to these sites, and transfection assays with promoter mutants of the MAdCAM-1 gene indicate that occupancy of both sites is essential for promoter function. The predominant NF-kappa B binding activity detected with these nuclear extracts is a p65 homodimer. These findings establish that, as with other endothelial cell adhesion molecules, transcriptional induction of MAdCAM-1 by TNF-alpha requires activated NF-kappa B proteins.

Phi Beta Kappa dissertation on the study of history at Harvard, by Daniel White and John Collins Warren, February 21, 1797

This dissertation, apparently delivered at a Phi Beta Kappa assembly on February 21, 1797 by Warren and White, concerns the study of history at Harvard College at the time they were students. In this manuscript version of their dissertation, Warren and White bemoan the insufficient attention paid to the discipline of history by the students and faculty at Harvard.

Arguments prepared by Daniel Appleton White for meetings of Phi Beta Kappa, 1796-1797

Three handwritten arguments prepared by Phi Beta Kappa member Daniel Appleton White (1776-1861; Harvard AB 1797) for discussion at meetings of the Alpha chapter at Harvard University in 1796 and 1797. The documents consist of a small paper notebook with a response to the prompt, "Whether the deeper studies, such as metaphysics, mathematics & natural philosophy, are entitled to our chief attention?" dated September 27, 1796, and prepared for debate with classmate Isaac Wellington (died 1797); a one-leaf document with a disputation on, "Whether civilized nations have a right to drive uncivilized nations from the lands they occupy?" dated December 8, 1796; and a small paper notebook containing White’s argument to the prompt, "Would a national university be beneficial for America?" that he debated with John Collins Warren (1778-1856; Harvard AB 1797) during the chapter’s May 16, 1797, meeting.

Lists of Phi Beta Kappa's new members, 1790, 1793, and 1797

Shapleigh served as Secretary of Harvard's chapter of Phi Beta Kappa in 1797.

Catalogue of the Harvard chapter of Phi beta kappa.

Mode of access: Internet.

Song book of the Kappa Alpha Theta Fraternity, 1890.

Mode of access: Internet.

Phi beta kappa directory, 1776-1941.

Mode of access: Internet.

The Phi Beta Kappa key.

"The official publication of the united chapters of Phi Beta Kappa."

The American legend. A poem before the Phi beta kappa society of Harvard university, July 18, 1850.

Original brown paper covers bound in.