La idea de unidad en tiempos del Congreso de 1816-1819

Este artículo se propone analizar el debate sobre la forma de gobierno que se dio en las sesiones del Congreso Constituyente y en la prensa porteña durante 1816, como así también la posterior discusión del proyecto de constitución. Estas polémicas dieron lugar a diversas posturas acerca del ejercicio de la soberanía -monarquía o república- como sobre la titularidad de la misma -pueblos/nación-. En ese marco mostraremos que los partidarios de la unidad intentaron establecerla mediante la implantación de una monarquía constitucional y la consagración de la nación única e indivisible como sujeto de imputación de la soberanía

Alternativas de análise para experimentos G × E multiatributo

Geralmente, nos experimentos genótipo por ambiente (G × E) é comum observar o comportamento dos genótipos em relação a distintos atributos nos ambientes considerados. A análise deste tipo de experimentos tem sido abordada amplamente para o caso de um único atributo. Nesta tese são apresentadas algumas alternativas de análise considerando genótipos, ambientes e atributos simultaneamente. A primeira, é baseada no método de mistura de máxima verossimilhança de agrupamento - Mixclus e a análise de componentes principais de 3 modos - 3MPCA, que permitem a análise de tabelas de tripla entrada, estes dois métodos têm sido muito usados na área da psicologia e da química, mas pouco na agricultura. A segunda, é uma metodologia que combina, o modelo de efeitos aditivos com interação multiplicativa - AMMI, modelo eficiente para a análise de experimentos (G × E) com um atributo e a análise de procrustes generalizada, que permite comparar configurações de pontos e proporcionar uma medida numérica de quanto elas diferem. Finalmente, é apresentada uma alternativa para realizar imputação de dados nos experimentos (G × E), pois, uma situação muito frequente nestes experimentos, é a presença de dados faltantes. Conclui-se que as metodologias propostas constituem ferramentas úteis para a análise de experimentos (G × E) multiatributo.

Imputação de dados em experimentos multiambientais: novos algoritmos utilizando a decomposição por valores singulares

As análises biplot que utilizam os modelos de efeitos principais aditivos com inter- ação multiplicativa (AMMI) requerem matrizes de dados completas, mas, frequentemente os ensaios multiambientais apresentam dados faltantes. Nesta tese são propostas novas metodologias de imputação simples e múltipla que podem ser usadas para analisar da- dos desbalanceados em experimentos com interação genótipo por ambiente (G×E). A primeira, é uma nova extensão do método de validação cruzada por autovetor (Bro et al, 2008). A segunda, corresponde a um novo algoritmo não-paramétrico obtido por meio de modificações no método de imputação simples desenvolvido por Yan (2013). Também é incluído um estudo que considera sistemas de imputação recentemente relatados na literatura e os compara com o procedimento clássico recomendado para imputação em ensaios (G×E), ou seja, a combinação do algoritmo de Esperança-Maximização com os modelos AMMI ou EM-AMMI. Por último, são fornecidas generalizações da imputação simples descrita por Arciniegas-Alarcón et al. (2010) que mistura regressão com aproximação de posto inferior de uma matriz. Todas as metodologias têm como base a decomposição por valores singulares (DVS), portanto, são livres de pressuposições distribucionais ou estruturais. Para determinar o desempenho dos novos esquemas de imputação foram realizadas simulações baseadas em conjuntos de dados reais de diferentes espécies, com valores re- tirados aleatoriamente em diferentes porcentagens e a qualidade das imputações avaliada com distintas estatísticas. Concluiu-se que a DVS constitui uma ferramenta útil e flexível na construção de técnicas eficientes que contornem o problema de perda de informação em matrizes experimentais.

Computational Methods for Identifying Hypertrophic Cardiomyopathy using 12-lead ECG

Hypertrophic cardiomyopathy (HCM) is a cardiovascular disease where the heart muscle is partially thickened and blood flow is - potentially fatally - obstructed. It is one of the leading causes of sudden cardiac death in young people. Electrocardiography (ECG) and Echocardiography (Echo) are the standard tests for identifying HCM and other cardiac abnormalities. The American Heart Association has recommended using a pre-participation questionnaire for young athletes instead of ECG or Echo tests due to considerations of cost and time involved in interpreting the results of these tests by an expert cardiologist. Initially we set out to develop a classifier for automated prediction of young athletes’ heart conditions based on the answers to the questionnaire. Classification results and further in-depth analysis using computational and statistical methods indicated significant shortcomings of the questionnaire in predicting cardiac abnormalities. Automated methods for analyzing ECG signals can help reduce cost and save time in the pre-participation screening process by detecting HCM and other cardiac abnormalities. Therefore, the main goal of this dissertation work is to identify HCM through computational analysis of 12-lead ECG. ECG signals recorded on one or two leads have been analyzed in the past for classifying individual heartbeats into different types of arrhythmia as annotated primarily in the MIT-BIH database. In contrast, we classify complete sequences of 12-lead ECGs to assign patients into two groups: HCM vs. non-HCM. The challenges and issues we address include missing ECG waves in one or more leads and the dimensionality of a large feature-set. We address these by proposing imputation and feature-selection methods. We develop heartbeat-classifiers by employing Random Forests and Support Vector Machines, and propose a method to classify full 12-lead ECGs based on the proportion of heartbeats classified as HCM. The results from our experiments show that the classifiers developed using our methods perform well in identifying HCM. Thus the two contributions of this thesis are the utilization of computational and statistical methods for discovering shortcomings in a current screening procedure and the development of methods to identify HCM through computational analysis of 12-lead ECG signals.

Genetic association of CD247 (CD3ζ) with SLE in a large-scale multiethnic study

A classic T-cell phenotype in systemic lupus erythematosus (SLE) is the downregulation and replacement of the CD3ζ chain that alters T-cell receptor signaling. However, genetic associations with SLE in the human CD247 locus that encodes CD3ζ are not well established and require replication in independent cohorts. Our aim was therefore to examine, localize and validate CD247-SLE association in a large multiethnic population. We typed 44 contiguous CD247 single-nucleotide polymorphisms (SNPs) in 8922 SLE patients and 8077 controls from four ethnically distinct populations. The strongest associations were found in the Asian population (11 SNPs in intron 1, 4.99 × 10(-4) < P < 4.15 × 10(-2)), where we further identified a five-marker haplotype (rs12141731-rs2949655-rs16859085-rs12144621-rs858554; G-G-A-G-A; P(hap) = 2.12 × 10(-5)) that exceeded the most associated single SNP rs858554 (minor allele frequency in controls = 13%; P = 4.99 × 10(-4), odds ratio = 1.32) in significance. Imputation and subsequent association analysis showed evidence of association (P < 0.05) at 27 additional SNPs within intron 1. Cross-ethnic meta-analysis, assuming an additive genetic model adjusted for population proportions, showed five SNPs with significant P-values (1.40 × 10(-3) < P< 3.97 × 10(-2)), with one (rs704848) remaining significant after Bonferroni correction (P(meta) = 2.66 × 10(-2)). Our study independently confirms and extends the association of SLE with CD247, which is shared by various autoimmune disorders and supports a common T-cell-mediated mechanism.

La idea de unidad en tiempos del Congreso de 1816-1819

Este artículo se propone analizar el debate sobre la forma de gobierno que se dio en las sesiones del Congreso Constituyente y en la prensa porteña durante 1816, como así también la posterior discusión del proyecto de constitución. Estas polémicas dieron lugar a diversas posturas acerca del ejercicio de la soberanía -monarquía o república- como sobre la titularidad de la misma -pueblos/nación-. En ese marco mostraremos que los partidarios de la unidad intentaron establecerla mediante la implantación de una monarquía constitucional y la consagración de la nación única e indivisible como sujeto de imputación de la soberanía

Reflexiones sobre ideología e interpelación en las elecciones presidenciales de 2015 en Argentina

En este trabajo abordamos del papel de la ideología y distintas formas de interpelación en las elecciones presidenciales de Argentina en 2015.Partimos de dos imputaciones cruzadas: la que surge del kirchnerismo, enfatizando el rol de los medios de comunicación masivos sobre los sectores medios y populares, y la respuesta progresista que insiste en una serie de motivos materiales de insatisfacción de estos sectores, a los que el kirchnerismo, afectado por una "miopía política", no atendió durante su gobierno y a los que desconoció en su discurso de campaña electoral, dejándolos librados a la interpelación por una forma de "individualismo miope". Insistimos en que estas atribuciones cruzadas de ideologismo constituyen un campo ideológico por sí mismo, cuyo presupuesto común es el individualismo. A partir de allí intentamos comprender las condiciones histórico-políticas en la Argentina del siglo XX que hacen del individualismo una evidencia ideológica fundante en la Argentina del siglo XXI

Reflexiones sobre ideología e interpelación en las elecciones presidenciales de 2015 en Argentina

En este trabajo abordamos del papel de la ideología y distintas formas de interpelación en las elecciones presidenciales de Argentina en 2015.Partimos de dos imputaciones cruzadas: la que surge del kirchnerismo, enfatizando el rol de los medios de comunicación masivos sobre los sectores medios y populares, y la respuesta progresista que insiste en una serie de motivos materiales de insatisfacción de estos sectores, a los que el kirchnerismo, afectado por una "miopía política", no atendió durante su gobierno y a los que desconoció en su discurso de campaña electoral, dejándolos librados a la interpelación por una forma de "individualismo miope". Insistimos en que estas atribuciones cruzadas de ideologismo constituyen un campo ideológico por sí mismo, cuyo presupuesto común es el individualismo. A partir de allí intentamos comprender las condiciones histórico-políticas en la Argentina del siglo XX que hacen del individualismo una evidencia ideológica fundante en la Argentina del siglo XXI

Risk of Cerebral Venous Thrombosis in Obese Women.

IMPORTANCE Obesity is a risk factor for deep vein thrombosis of the leg and pulmonary embolism. To date, however, whether obesity is associated with adult cerebral venous thrombosis (CVT) has not been assessed. OBJECTIVE To assess whether obesity is a risk factor for CVT. DESIGN, SETTING, AND PARTICIPANTS A case-control study was performed in consecutive adult patients with CVT admitted from July 1, 2006 (Amsterdam), and October 1, 2009 (Berne), through December 31, 2014, to the Academic Medical Center in Amsterdam, the Netherlands, or Inselspital University Hospital in Berne, Switzerland. The control group was composed of individuals from the control population of the Multiple Environmental and Genetic Assessment of Risk Factors for Venous Thrombosis study, which was a large Dutch case-control study performed from March 1, 1999, to September 31, 2004, and in which risk factors for deep vein thrombosis and pulmonary embolism were assessed. Data analysis was performed from January 2 to July 12, 2015. MAIN OUTCOMES AND MEASURES Obesity was determined by body mass index (BMI). A BMI of 30 or greater was considered to indicate obesity, and a BMI of 25 to 29.99 was considered to indicate overweight. A multiple imputation procedure was used for missing data. We adjusted for sex, age, history of cancer, ethnicity, smoking status, and oral contraceptive use. Individuals with normal weight (BMI <25) were the reference category. RESULTS The study included 186 cases and 6134 controls. Cases were younger (median age, 40 vs 48 years), more often female (133 [71.5%] vs 3220 [52.5%]), more often used oral contraceptives (97 [72.9%] vs 758 [23.5%] of women), and more frequently had a history of cancer (17 [9.1%] vs 235 [3.8%]) compared with controls. Obesity (BMI ≥30) was associated with an increased risk of CVT (adjusted odds ratio [OR], 2.63; 95% CI, 1.53-4.54). Stratification by sex revealed a strong association between CVT and obesity in women (adjusted OR, 3.50; 95% CI, 2.00-6.14) but not in men (adjusted OR, 1.16; 95% CI, 0.25-5.30). Further stratification revealed that, in women who used oral contraceptives, overweight and obesity were associated with an increased risk of CVT in a dose-dependent manner (BMI 25.0-29.9: adjusted OR, 11.87; 95% CI, 5.94-23.74; BMI ≥30: adjusted OR, 29.26; 95% CI, 13.47-63.60). No association was found in women who did not use oral contraceptives. CONCLUSIONS AND RELEVANCE Obesity is a strong risk factor for CVT in women who use oral contraceptives.

Contribution of Genetic Background and Clinical Risk Factors to Low-Trauma Fractures in Human Immunodeficiency Virus (HIV)-Positive Persons: The Swiss HIV Cohort Study.

Background.  The impact of human genetic background on low-trauma fracture (LTF) risk has not been evaluated in the context of human immunodeficiency virus (HIV) and clinical LTF risk factors. Methods.  In the general population, 6 common single-nucleotide polymorphisms (SNPs) associate with LTF through genome-wide association study. Using genome-wide SNP arrays and imputation, we genotyped these SNPs in HIV-positive, white Swiss HIV Cohort Study participants. We included 103 individuals with a first, physician-validated LTF and 206 controls matched on gender, whose duration of observation and whose antiretroviral therapy start dates were similar using incidence density sampling. Analyses of nongenetic LTF risk factors were based on 158 cases and 788 controls. Results.  A genetic risk score built from the 6 LTF-associated SNPs did not associate with LTF risk, in both models including and not including parental hip fracture history. The contribution of clinical LTF risk factors was limited in our dataset. Conclusions.  Genetic LTF markers with a modest effect size in the general population do not improve fracture prediction in persons with HIV, in whom clinical LTF risk factors are prevalent in both cases and controls.

A treatise of justifying righteousness : in two books ... ; all published instead of a fuller answer to the assaults in Dr. Tullies Justificatio Paulina ... /

Pages 306, 307, and 310 of part 3 are misnumbered as 290, 291 and 294 respectively.

La idea de unidad en tiempos del Congreso de 1816-1819

Este artículo se propone analizar el debate sobre la forma de gobierno que se dio en las sesiones del Congreso Constituyente y en la prensa porteña durante 1816, como así también la posterior discusión del proyecto de constitución. Estas polémicas dieron lugar a diversas posturas acerca del ejercicio de la soberanía -monarquía o república- como sobre la titularidad de la misma -pueblos/nación-. En ese marco mostraremos que los partidarios de la unidad intentaron establecerla mediante la implantación de una monarquía constitucional y la consagración de la nación única e indivisible como sujeto de imputación de la soberanía

Reflexiones sobre ideología e interpelación en las elecciones presidenciales de 2015 en Argentina

En este trabajo abordamos del papel de la ideología y distintas formas de interpelación en las elecciones presidenciales de Argentina en 2015.Partimos de dos imputaciones cruzadas: la que surge del kirchnerismo, enfatizando el rol de los medios de comunicación masivos sobre los sectores medios y populares, y la respuesta progresista que insiste en una serie de motivos materiales de insatisfacción de estos sectores, a los que el kirchnerismo, afectado por una "miopía política", no atendió durante su gobierno y a los que desconoció en su discurso de campaña electoral, dejándolos librados a la interpelación por una forma de "individualismo miope". Insistimos en que estas atribuciones cruzadas de ideologismo constituyen un campo ideológico por sí mismo, cuyo presupuesto común es el individualismo. A partir de allí intentamos comprender las condiciones histórico-políticas en la Argentina del siglo XX que hacen del individualismo una evidencia ideológica fundante en la Argentina del siglo XXI

Disentangling the impact of seroconversion age and set-point viral load on ART-free HIV survival

Thesis (Master's)--University of Washington, 2016-06

Analysis of Melanoma Onset: Assessing Familial Aggregation by Using Estimating Equations and Fitting Variance Components via Bayesian Random Effects Models

We investigate whether relative contributions of genetic and shared environmental factors are associated with an increased risk in melanoma. Data from the Queensland Familial Melanoma Project comprising 15,907 subjects arising from 1912 families were analyzed to estimate the additive genetic, common and unique environmental contributions to variation in the age at onset of melanoma. Two complementary approaches for analyzing correlated time-to-onset family data were considered: the generalized estimating equations (GEE) method in which one can estimate relationship-specific dependence simultaneously with regression coefficients that describe the average population response to changing covariates; and a subject-specific Bayesian mixed model in which heterogeneity in regression parameters is explicitly modeled and the different components of variation may be estimated directly. The proportional hazards and Weibull models were utilized, as both produce natural frameworks for estimating relative risks while adjusting for simultaneous effects of other covariates. A simple Markov Chain Monte Carlo method for covariate imputation of missing data was used and the actual implementation of the Bayesian model was based on Gibbs sampling using the free ware package BUGS. In addition, we also used a Bayesian model to investigate the relative contribution of genetic and environmental effects on the expression of naevi and freckles, which are known risk factors for melanoma.