Elisa Stünzner als Agathe, Rollenbild, Hüftbild, in: Carl Maria von Weber: Der Freischütz

Signatur des Originals: S 36/F06788

Elisa Stünzner als Micae͏̈la, Rollenbild, Brustbild, in: Georges Bizet: Carmen

Signatur des Originals: S 36/F06789

Elisa Stünzner als Mignon, Rollenbild, Kniestück, in: Ambroise Thomas: Mignon

Signatur des Originals: S 36/F06790

Elisa Stünzner als Senta, Rollenbild, Hüftbild, in: Richard Wagner: Der fliegende Holländer

Signatur des Originals: S 36/F06791

Elisa Stünzner als Senta, Rollenbild, Hüftbild, in: Richard Wagner: Der fliegende Holländer

Signatur des Originals: S 36/F06792

Elisa Stünzner als Eva, Rollenbild, Brustbild, in: Richard Wagner: Die Meistersinger von Nürnberg

Signatur des Originals: S 36/F06793

Elisa Stünzner, Rollenbild, in: Richard Strauss: Der Rosenkavalier

Signatur des Originals: S 36/F06794

Elisa Stünzner, Rollenbild, Brustbild, in: Richard Strauss: Der Rosenkavalier

Signatur des Originals: S 36/F06795

Elisa Stünzner, Rollenbild, in: Richard Strauss: Der Rosenkavalier

Signatur des Originals: S 36/F06796

Elisa Blaes, Kniestück

Signatur des Originals: S 36/G01601

Elisa Blaes, Kniestück

Signatur des Originals: S 36/G01602

Elisa Pezold, Brustbild

Signatur des Originals: S 36/G01893

DETECTION OF MALARIAL SPOROZOITES BY THE ENZYME LINKED IMMUNOSORBENT ASSAY (ELISA)

Detection of malarial sporozoites by a double antibody sandwich enzyme linked immunosorbent assay (ELISA) is described. This investigation utilized the Anopheles stephensi-Plasmodium berghei malaria model for the generation of sporozoites. Anti-sporozoite antibody was obtained from the sera of rats which had been bitten by An. stephensi with salivary gland sporozoites. Mosquitoes were irradiated prior to feeding on the rats to render the sporozoites non-viable.^ The assay employed microtiter plates coated with their rat anti-sporozoite antiserum or rat anti-sporozoite IgG. Intact and sonicated sporozoites were used as antigens. Initially, sporozoites were detected by an ELISA using staphylococcal protein A conjugated with alkaline phosphatase. Sporozoites were also detected using alkaline phosphatase or horseradish peroxidase conjugated to anti-sporozoite IgG. Best results were obtained using the alkaline phosphatase conjugate.^ This investigation included the titration of antigen, coating antibody and labelled antibody as well as studies of various incubation times. A radioimmunoassay (RIA) was also developed and compared with the ELISA for detecting sporozoites. Finally, the detection of a single infected mosquito in pools of 5 to 10 whole, uninfested ones was studied using both ELISA and RIA.^ Sonicated sporozoites were more readily detected than intact sporozoites. The lower limit of detection was approximately 500 sporozoites per ml. Results using ELISA or RIA were similar. The ability of the ELISA to detect a single infected mosquito in a pool of uninfected ones indicates that this technique has potential use in entomological field studies which aim at determining the vector status of anopheline mosquitoes. The potential of the ELISA for identifying sporozoites of different species of malaria is discussed. ^

Elisa Moyano (coord.). La literatura de Salta. Espacios de reconocimiento y formas de olvido. Salta, Universidad Nacional de Salta, 2005, 210 p.

Fil: Zapata Viani, María Alejandra.

A.A.V.V.: Utopía y representación literaria en obras francesas y francófonas (2010). Coord. por Lilia Elisa Castañón y Silvia Calí. Mendoza: Facultad de Filosofía y Letras, Universidad Nacional de Cuyo. 21 x 25 cm. 303 p.

El volumen que reseñamos constituye las ACTAS de las XXIII Jornadas Nacionales de Literatura Francesa y Francófona que se llevaron a cabo en Mendoza, en la sede de la Facultad de Filosofía y Letras de la U.N.Cuyo, los días 27, 28 y 29 de mayo de 2010.