Proposta de equipamento de conexão à rede para a utilização da geração de energia solar em consumidores de pequeno e médio porte

Pós-graduação em Engenharia Mecânica - FEG

Polymeric Nanoparticles as Oral Delivery Systems for Encapsulation and Release of Polyphenolic Compounds: Impact on Quercetin Antioxidant Activity & Bioaccessibility

A delivery system containing polymeric (Eudragit) nanoparticles has been developed for encapsulation and controlled release of bioactive flavonoids (quercetin). Nanoparticles were fabricated using a solvent displacement method. Particle size, morphology, and charge were measured by light scattering, electron microscopy and zeta-potential. Encapsulation efficiency (EE) and release profiles were determined using electrochemical methods. Molecular interactions within the particle matrix were characterized by X-ray diffraction, differential scanning calorimetry, and infrared spectroscopy. Antioxidant properties of free and encapsulated quercetin were analyzed by TBARS and fluorescence spectroscopy. Bioaccessibility of quercetin was evaluated using an in vitro digestion model. Relatively small (d a parts per thousand aEuro parts per thousand 370 nm) anionic polymeric nanoparticles were formed containing quercetin in a non-crystalline form (EE a parts per thousand aEuro parts per thousand 67 %). The main interaction between quercetin and Eudragit was hydrogen bonding. Encapsulated quercetin remained stable during 6 months storage and maintained its antioxidant activity. Quercetin bioaccessibility within simulated small intestinal conditions was improved by encapsulation. The knowledge obtained from this study will facilitate the rational design and fabrication of polymeric nanoparticles as oral delivery systems for encapsulation, protection, and release of bioactive compounds.

Design and Validation of an Optimized MB-OFDM Ultra Wideband Transceiver System

Esta tesis está incluida dentro del campo del campo de Multiband Orthogonal Frequency Division Multiplexing Ultra Wideband (MB-OFDM UWB), el cual ha adquirido una gran importancia en las comunicaciones inalámbricas de alta tasa de datos en la última década. UWB surgió con el objetivo de satisfacer la creciente demanda de conexiones inalámbricas en interiores y de uso doméstico, con bajo coste y alta velocidad. La disponibilidad de un ancho de banda grande, el potencial para alta velocidad de transmisión, baja complejidad y bajo consumo de energía, unido al bajo coste de implementación, representa una oportunidad única para que UWB se convierta en una solución ampliamente utilizada en aplicaciones de Wireless Personal Area Network (WPAN). UWB está definido como cualquier transmisión que ocupa un ancho de banda de más de 20% de su frecuencia central, o más de 500 MHz. En 2002, la Comisión Federal de Comunicaciones (FCC) definió que el rango de frecuencias de transmisión de UWB legal es de 3.1 a 10.6 GHz, con una energía de transmisión de -41.3 dBm/Hz. Bajo las directrices de FCC, el uso de la tecnología UWB puede aportar una enorme capacidad en las comunicaciones de corto alcance. Considerando las ecuaciones de capacidad de Shannon, incrementar la capacidad del canal requiere un incremento lineal en el ancho de banda, mientras que un aumento similar de la capacidad de canal requiere un aumento exponencial en la energía de transmisión. En los últimos años, s diferentes desarrollos del UWB han sido extensamente estudiados en diferentes áreas, entre los cuales, el protocolo de comunicaciones inalámbricas MB-OFDM UWB está considerado como la mejor elección y ha sido adoptado como estándar ISO/IEC para los WPANs. Combinando la modulación OFDM y la transmisión de datos utilizando las técnicas de salto de frecuencia, el sistema MB-OFDM UWB es capaz de soportar tasas de datos con que pueden variar de los 55 a los 480 Mbps, alcanzando una distancia máxima de hasta 10 metros. Se esperara que la tecnología MB-OFDM tenga un consumo energético muy bajo copando un are muy reducida en silicio, proporcionando soluciones de bajo coste que satisfagan las demandas del mercado. Para cumplir con todas estas expectativas, el desarrollo y la investigación del MBOFDM UWB deben enfrentarse a varios retos, como son la sincronización de alta sensibilidad, las restricciones de baja complejidad, las estrictas limitaciones energéticas, la escalabilidad y la flexibilidad. Tales retos requieren un procesamiento digital de la señal de última generación, capaz de desarrollar sistemas que puedan aprovechar por completo las ventajas del espectro UWB y proporcionar futuras aplicaciones inalámbricas en interiores. Esta tesis se centra en la completa optimización de un sistema de transceptor de banda base MB-OFDM UWB digital, cuyo objetivo es investigar y diseñar un subsistema de comunicación inalámbrica para la aplicación de las Redes de Sensores Inalámbricas Visuales. La complejidad inherente de los procesadores FFT/IFFT y el sistema de sincronización así como la alta frecuencia de operación para todos los elementos de procesamiento, se convierten en el cuello de la botella para el diseño y la implementación del sistema de UWB digital en base de banda basado en MB-OFDM de baja energía. El objetivo del transceptor propuesto es conseguir baja energía y baja complejidad bajo la premisa de un alto rendimiento. Las optimizaciones están realizadas tanto a nivel algorítmico como a nivel arquitectural para todos los elementos del sistema. Una arquitectura hardware eficiente en consumo se propone en primer lugar para aquellos módulos correspondientes a núcleos de computación. Para el procesado de la Transformada Rápida de Fourier (FFT/IFFT), se propone un algoritmo mixed-radix, basado en una arquitectura con pipeline y se ha desarrollado un módulo de Decodificador de Viterbi (VD) equilibrado en coste-velocidad con el objetivo de reducir el consumo energético e incrementar la velocidad de procesamiento. También se ha implementado un correlador signo-bit simple basado en la sincronización del tiempo de símbolo es presentado. Este correlador es usado para detectar y sincronizar los paquetes de OFDM de forma robusta y precisa. Para el desarrollo de los subsitemas de procesamiento y realizar la integración del sistema completo se han empleado tecnologías de última generación. El dispositivo utilizado para el sistema propuesto es una FPGA Virtex 5 XC5VLX110T del fabricante Xilinx. La validación el propuesta para el sistema transceptor se ha implementado en dicha placa de FPGA. En este trabajo se presenta un algoritmo, y una arquitectura, diseñado con filosofía de co-diseño hardware/software para el desarrollo de sistemas de FPGA complejos. El objetivo principal de la estrategia propuesta es de encontrar una metodología eficiente para el diseño de un sistema de FPGA configurable optimizado con el empleo del mínimo esfuerzo posible en el sistema de procedimiento de verificación, por tanto acelerar el periodo de desarrollo del sistema. La metodología de co-diseño presentada tiene la ventaja de ser fácil de usar, contiene todos los pasos desde la propuesta del algoritmo hasta la verificación del hardware, y puede ser ampliamente extendida para casi todos los tipos de desarrollos de FPGAs. En este trabajo se ha desarrollado sólo el sistema de transceptor digital de banda base por lo que la comprobación de señales transmitidas a través del canal inalámbrico en los entornos reales de comunicación sigue requiriendo componentes RF y un front-end analógico. No obstante, utilizando la metodología de co-simulación hardware/software citada anteriormente, es posible comunicar el sistema de transmisor y el receptor digital utilizando los modelos de canales propuestos por IEEE 802.15.3a, implementados en MATLAB. Por tanto, simplemente ajustando las características de cada modelo de canal, por ejemplo, un incremento del retraso y de la frecuencia central, podemos estimar el comportamiento del sistema propuesto en diferentes escenarios y entornos. Las mayores contribuciones de esta tesis son: • Se ha propuesto un nuevo algoritmo 128-puntos base mixto FFT usando la arquitectura pipeline multi-ruta. Los complejos multiplicadores para cada etapa de procesamiento son diseñados usando la arquitectura modificada shiftadd. Los sistemas word length y twiddle word length son comparados y seleccionados basándose en la señal para cuantización del SQNR y el análisis de energías. • El desempeño del procesador IFFT es analizado bajo diferentes situaciones aritméticas de bloques de punto flotante (BFP) para el control de desbordamiento, por tanto, para encontrar la arquitectura perfecta del algoritmo IFFT basado en el procesador FFT propuesto. • Para el sistema de receptor MB-OFDM UWB se ha empleado una sincronización del tiempo innovadora, de baja complejidad y esquema de compensación, que consiste en funciones de Detector de Paquetes (PD) y Estimación del Offset del tiempo. Simplificando el cross-correlation y maximizar las funciones probables solo a sign-bit, la complejidad computacional se ve reducida significativamente. • Se ha propuesto un sistema de decodificadores Viterbi de 64 estados de decisión-débil usando velocidad base-4 de arquitectura suma-comparaselecciona. El algoritmo Two-pointer Even también es introducido en la unidad de rastreador de origen con el objetivo de conseguir la eficiencia en el hardware. • Se han integrado varias tecnologías de última generación en el completo sistema transceptor basebanda , con el objetivo de implementar un sistema de comunicación UWB altamente optimizado. • Un diseño de flujo mejorado es propuesto para el complejo sistema de implementación, el cual puede ser usado para diseños de Cadena de puertas de campo programable general (FPGA). El diseño mencionado no sólo reduce dramáticamente el tiempo para la verificación funcional, sino también provee un análisis automático como los errores del retraso del output para el sistema de hardware implementado. • Un ambiente de comunicación virtual es establecido para la validación del propuesto sistema de transceptores MB-OFDM. Este método es provisto para facilitar el uso y la conveniencia de analizar el sistema digital de basebanda sin parte frontera analógica bajo diferentes ambientes de comunicación. Esta tesis doctoral está organizada en seis capítulos. En el primer capítulo se encuentra una breve introducción al campo del UWB, tanto relacionado con el proyecto como la motivación del desarrollo del sistema de MB-OFDM. En el capítulo 2, se presenta la información general y los requisitos del protocolo de comunicación inalámbrica MBOFDM UWB. En el capítulo 3 se habla de la arquitectura del sistema de transceptor digital MB-OFDM de banda base . El diseño del algoritmo propuesto y la arquitectura para cada elemento del procesamiento está detallado en este capítulo. Los retos de diseño del sistema que involucra un compromiso de discusión entre la complejidad de diseño, el consumo de energía, el coste de hardware, el desempeño del sistema, y otros aspectos. En el capítulo 4, se ha descrito la co-diseñada metodología de hardware/software. Cada parte del flujo del diseño será detallado con algunos ejemplos que se ha hecho durante el desarrollo del sistema. Aprovechando esta estrategia de diseño, el procedimiento de comunicación virtual es llevado a cabo para probar y analizar la arquitectura del transceptor propuesto. Los resultados experimentales de la co-simulación y el informe sintético de la implementación del sistema FPGA son reflejados en el capítulo 5. Finalmente, en el capítulo 6 se incluye las conclusiones y los futuros proyectos, y también los resultados derivados de este proyecto de doctorado. ABSTRACT In recent years, the Wireless Visual Sensor Network (WVSN) has drawn great interest in wireless communication research area. They enable a wealth of new applications such as building security control, image sensing, and target localization. However, nowadays wireless communication protocols (ZigBee, Wi-Fi, and Bluetooth for example) cannot fully satisfy the demands of high data rate, low power consumption, short range, and high robustness requirements. New communication protocol is highly desired for such kind of applications. The Ultra Wideband (UWB) wireless communication protocol, which has increased in importance for high data rate wireless communication field, are emerging as an important topic for WVSN research. UWB has emerged as a technology that offers great promise to satisfy the growing demand for low-cost, high-speed digital wireless indoor and home networks. The large bandwidth available, the potential for high data rate transmission, and the potential for low complexity and low power consumption, along with low implementation cost, all present a unique opportunity for UWB to become a widely adopted radio solution for future Wireless Personal Area Network (WPAN) applications. UWB is defined as any transmission that occupies a bandwidth of more than 20% of its center frequency, or more than 500 MHz. In 2002, the Federal Communications Commission (FCC) has mandated that UWB radio transmission can legally operate in the range from 3.1 to 10.6 GHz at a transmitter power of －41.3 dBm/Hz. Under the FCC guidelines, the use of UWB technology can provide enormous capacity over short communication ranges. Considering Shannon’s capacity equations, increasing the channel capacity requires linear increasing in bandwidth, whereas similar channel capacity increases would require exponential increases in transmission power. In recent years, several different UWB developments has been widely studied in different area, among which, the MB-OFDM UWB wireless communication protocol is considered to be the leading choice and has recently been adopted in the ISO/IEC standard for WPANs. By combing the OFDM modulation and data transmission using frequency hopping techniques, the MB-OFDM UWB system is able to support various data rates, ranging from 55 to 480 Mbps, over distances up to 10 meters. The MB-OFDM technology is expected to consume very little power and silicon area, as well as provide low-cost solutions that can satisfy consumer market demands. To fulfill these expectations, MB-OFDM UWB research and development have to cope with several challenges, which consist of high-sensitivity synchronization, low- complexity constraints, strict power limitations, scalability, and flexibility. Such challenges require state-of-the-art digital signal processing expertise to develop systems that could fully take advantages of the UWB spectrum and support future indoor wireless applications. This thesis focuses on fully optimization for the MB-OFDM UWB digital baseband transceiver system, aiming at researching and designing a wireless communication subsystem for the Wireless Visual Sensor Networks (WVSNs) application. The inherent high complexity of the FFT/IFFT processor and synchronization system, and high operation frequency for all processing elements, becomes the bottleneck for low power MB-OFDM based UWB digital baseband system hardware design and implementation. The proposed transceiver system targets low power and low complexity under the premise of high performance. Optimizations are made at both algorithm and architecture level for each element of the transceiver system. The low-power hardwareefficient structures are firstly proposed for those core computation modules, i.e., the mixed-radix algorithm based pipelined architecture is proposed for the Fast Fourier Transform (FFT/IFFT) processor, and the cost-speed balanced Viterbi Decoder (VD) module is developed, in the aim of lowering the power consumption and increasing the processing speed. In addition, a low complexity sign-bit correlation based symbol timing synchronization scheme is presented so as to detect and synchronize the OFDM packets robustly and accurately. Moreover, several state-of-the-art technologies are used for developing other processing subsystems and an entire MB-OFDM digital baseband transceiver system is integrated. The target device for the proposed transceiver system is Xilinx Virtex 5 XC5VLX110T FPGA board. In order to validate the proposed transceiver system in the FPGA board, a unified algorithm-architecture-circuit hardware/software co-design environment for complex FPGA system development is presented in this work. The main objective of the proposed strategy is to find an efficient methodology for designing a configurable optimized FPGA system by using as few efforts as possible in system verification procedure, so as to speed up the system development period. The presented co-design methodology has the advantages of easy to use, covering all steps from algorithm proposal to hardware verification, and widely spread for almost all kinds of FPGA developments. Because only the digital baseband transceiver system is developed in this thesis, the validation of transmitting signals through wireless channel in real communication environments still requires the analog front-end and RF components. However, by using the aforementioned hardware/software co-simulation methodology, the transmitter and receiver digital baseband systems get the opportunity to communicate with each other through the channel models, which are proposed from the IEEE 802.15.3a research group, established in MATLAB. Thus, by simply adjust the characteristics of each channel model, e.g. mean excess delay and center frequency, we can estimate the transmission performance of the proposed transceiver system through different communication situations. The main contributions of this thesis are: • A novel mixed radix 128-point FFT algorithm by using multipath pipelined architecture is proposed. The complex multipliers for each processing stage are designed by using modified shift-add architectures. The system wordlength and twiddle word-length are compared and selected based on Signal to Quantization Noise Ratio (SQNR) and power analysis. • IFFT processor performance is analyzed under different Block Floating Point (BFP) arithmetic situations for overflow control, so as to find out the perfect architecture of IFFT algorithm based on the proposed FFT processor. • An innovative low complex timing synchronization and compensation scheme, which consists of Packet Detector (PD) and Timing Offset Estimation (TOE) functions, for MB-OFDM UWB receiver system is employed. By simplifying the cross-correlation and maximum likelihood functions to signbit only, the computational complexity is significantly reduced. • A 64 state soft-decision Viterbi Decoder system by using high speed radix-4 Add-Compare-Select architecture is proposed. Two-pointer Even algorithm is also introduced into the Trace Back unit in the aim of hardware-efficiency. • Several state-of-the-art technologies are integrated into the complete baseband transceiver system, in the aim of implementing a highly-optimized UWB communication system. • An improved design flow is proposed for complex system implementation which can be used for general Field-Programmable Gate Array (FPGA) designs. The design method not only dramatically reduces the time for functional verification, but also provides automatic analysis such as errors and output delays for the implemented hardware systems. • A virtual communication environment is established for validating the proposed MB-OFDM transceiver system. This methodology is proved to be easy for usage and convenient for analyzing the digital baseband system without analog frontend under different communication environments. This PhD thesis is organized in six chapters. In the chapter 1 a brief introduction to the UWB field, as well as the related work, is done, along with the motivation of MBOFDM system development. In the chapter 2, the general information and requirement of MB-OFDM UWB wireless communication protocol is presented. In the chapter 3, the architecture of the MB-OFDM digital baseband transceiver system is presented. The design of the proposed algorithm and architecture for each processing element is detailed in this chapter. Design challenges of such system involve trade-off discussions among design complexity, power consumption, hardware cost, system performance, and some other aspects. All these factors are analyzed and discussed. In the chapter 4, the hardware/software co-design methodology is proposed. Each step of this design flow will be detailed by taking some examples that we met during system development. Then, taking advantages of this design strategy, the Virtual Communication procedure is carried out so as to test and analyze the proposed transceiver architecture. Experimental results from the co-simulation and synthesis report of the implemented FPGA system are given in the chapter 5. The chapter 6 includes conclusions and future work, as well as the results derived from this PhD work.

Sistema de e-learning en el entorno AFRICA BUILD Portal

Diversos estudios consideran que las claves para el desarrollo técnico, social y económico de los países en desarrollo, y en especial en África, son la educación, la investigación y la innovación. No obstante, en el ámbito sanitario en particular, la formación e investigación en África sigue siendo escasa por diversos motivos. Por un lado, los investigadores africanos encuentran dificultades añadidas para obtener financiación frente a investigadores en países de desarrollo. Y, por otro lado, los investigadores no disponen de las herramientas y tecnologías adecuadas para acceder a información relevante para su trabajo. En este contexto, el proyecto AFRICA BUILD tiene como principal objetivo mejorar y fomentar la investigación y educación sanitaria en África a través de las nuevas tecnologías. Uno de los resultados principales de este proyecto es el AFRICA BUILD Portal (ABP), que pretende ser un punto de encuentro para los investigadores africanos, donde podrán encontrar un amplio abanico de herramientas que les permita no sólo mejorar su educación, sino compartir conocimientos con otros investigadores. Este portal, que está actualmente en desarrollo, pretende fomentar la comunicación entre instituciones africanas y les proveerá herramientas biomédicas avanzadas. En este Trabajo de Fin de Grado se han marcado como principal objetivo la mejora del sistema de e-learning que existía en el ABP. El nuevo sistema será: (i) más potente, óptimo y rico en funcionalidades, (ii) tendrá una importante base social permitiendo a los usuarios aportar información y compartir conocimiento, y (iii) será distribuido y escalable, capaz de integrar distintos gestores de contenidos didácticos como fuentes de información. Los resultados obtenidos son positivos y el nuevo sistema de aprendizaje ha mejorado la usabilidad, funcionalidad y apariencia del anterior. Para concluir, la herramienta posee aún un gran margen de mejora, pero gracias al trabajo realizado el sistema de e-learning es ahora más social, distribuido, escalable e innovador. ---ABSTRACT---Several studies find that the keys to the technical, social and economic development of developing countries, especially in Africa, are education, research and innovation. However, in the health sector in particular, training and research in Africa remains low for various reasons. On the one hand, African researchers experience additional difficulties for funding against researchers in developing countries. And on the other hand, researchers do not have the right tools and technologies to access information relevant to their work. In this context, the AFRICA BUILD project's main objective is to improve and enhance research and health education in Africa through new technologies. One of the main results of this project is the AFRICA BUILD Portal (ABP), which aims to be a meeting point for African researchers, where they can find a wide range of tools that allows them to not only improve their education but to share knowledge with other researchers. This portal, which is currently under development, aims to promote communication between African institutions and will provide advanced biomedical tools. The main objective of this Final Year Project is the improvement of the former elearning system that already existed in the ABP. The new system will be: (i) more powerful, richer in features, (ii) will have an important social base allowing users to contribute information and knowledge sharing; and (iii) will be distributed and scalable, capable of integrating different learning management systems as sources of information. The final results obtained are fairly positive and the new e-learning system has improved its usability, functionality and appearance of the former. To conclude, the tool still has much room for improvement, but thanks to the new features of the improved elearning system has become more social, distributed, scalable and innovative.

Monitorización de la integración de sistemas intensivos software

El software se ha convertido en el eje central del mundo actual, una compleja creación humana que influye en la vida, negocios y comunicación de todas las personas pertenecientes a la Sociedad de la Información. El rápido crecimiento experimentado en el ámbito del desarrollo software ha permitido la creación de avanzadas estructuras tecnológicas, denominadas “Sistemas Intensivos Software”, capaces de comunicarse con otros sistemas, dispositivos, sensores y personas. A lo largo de los próximos años los sistemas se enfrentarán a una mayor complejidad, surgida de la necesidad de operar en entornos de grandes dimensiones y de comportamientos no deterministas. Los métodos y herramientas actuales no son lo suficientemente potentes para diseñar, construir,implementar y mantener sistemas intensivos software con estas características, y detener la construcción de sistemas intensivos software o construir sistemas poco flexibles o fiables no es una alternativa real. En el desarrollo de “Sistemas Intensivos Software” pueden llegar a intervenir distintas entidades o compañías software que suelen estar en ubicaciones geográficas distintas y constituidas por grandes equipos de desarrollo, multidisciplinares e incluso multilingües. Debido a la criticidad del resultado de las actividades realizadas de forma independiente en el sistema resultante, éstas se han de controlar y monitorizar para asegurar la correcta integración de todos los elementos del sistema completo. El objetivo de este proyecto es la creación de una herramienta software para dar soporte a la gestión y monitorización de la construcción e integración de sistemas intensivos software, siendo extensible también a proyectos de otra índole. La herramienta resultante se denomina Positioning System, una aplicación web del tipo SPA (Single Page Application) creada con tecnología de última generación como el framework JavaScript AngularJS y tecnología de back-end como SlimPHP. Positioning System provee la funcionalidad necesaria para la creación de proyectos, familias y subfamilias de productos que constituyen los productos software de los proyectos creados, así como la gestión de socios comerciales y gestión de contactos de dichos proyectos. Todas estas funcionalidades son fácilmente monitorizadas y controladas por gráficos estadísticos generados para cada proyecto. ABSTRACT Software has become the backbone of today’s world, a complex human creation that has an important impact in the life, business and communication of all people involved with the Information Society. The quick growth that software development has undergone for last years has enabled the creation of advanced technological structures called “Software Intensive Systems”. They are able to communicate with other systems, devices, sensors and people. Next years, systems will face more complexity. It arises from the need of operating systems of large dimensions with non-deterministic behaviors. Current methods and tools are not powerful enough to design, build, implement and maintain software intensive systems; however stopping the development or developing unreliable and non-flexible systems is not a real alternative. Software Intensive Systems” development may involve different entities or software companies which may be in different geographical locations and may be constituted by large, multidisciplinary and even multilingual development teams. Due to the criticality of the result of each conducted activity, independently in the resulting system, these activities must be controlled and monitored to ensure the proper integration of all the elements within the complete system. The goal of this project is the creation of a software tool to support the management and monitoring of the construction and integration of software intensive systems, being possible to be extended to other kind of projects. The resultant tool is called Positioning System, a web application that follows the SPA (Single Page Application) style. It was created with the latest technologies, such as, the AngularJS framework and SlimPHP. The Positioning System provides the necessary features for the creation of projects, families and subfamilies of products that constitute the software products of the created projects, as well as the management of business partners and contacts of these projects. All these features are easily monitored and controlled by statistical graphs generated for each project.

Prototipos experimentales modernos: la colonia de Schorlemerallee y las villas Am Rupenhorn de los Luckhardt y Anker. Berlín 1924-1930

La colonia experimental de Schorlemerallee y las villas Am Rupenhorn son dos proyectos concluidos en 1930 por los hermanos Wassili y Hans Luckhardt con Alfons Anker en Berlín. Ambos proyectos forman parte del mismo proceso, que comienza en la Colonia -una exploración sobre el lenguaje moderno en una serie de fases sucesivas- y culmina con las Villas. Éstas últimas, realizadas inmediatamente después de la Colonia, son la síntesis de esa experiencia, aunque finalmente acabaron trascendiéndola, ya que se convirtieron en un modelo sobre la casa en la naturaleza, sobre la idea de la villa clásica y sobre los nuevos modos de habitar, alcanzando con el tiempo la condición de canon moderno. A pesar de ello, no es esta condición lo más importante. Lo singular en este caso, es el propio proceso de proyecto –Colonia versus Villas- un verdadero experimento en su concepción, método y resultados, a través del cual sus autores investigan nuevas tecnologías aplicadas a nuevas formas de habitar y desarrollan un nuevo lenguaje, cuyo resultado son unos prototipos tecnológicos, con los que pretenden, como diría Mies van der Rohe: “Me he esforzado por construir una arquitectura para una sociedad tecnológica. He intentado que todo resultara razonable y claro.....para que cualquiera pueda hacer arquitectura.” El momento y lugar no pueden ser más propicios: Berlín entre 1924 y 1930, en el mismo origen del Movimiento Moderno. El experimento se plantea con auténtico rigor científico. Los arquitectos diseñan, construyen y financian su proyecto, controlando todas sus variables. Especialmente, por lo insólito, es el control de la variable económica. Porque este factor, la economía, es para ellos una clave fundamental del proceso. Se trataba de demostrar que la Nueva Arquitectura (o Neues Bauen, como les gustaba denominarla) era capaz de construir mejor y más rápido la vivienda para una nueva sociedad. La revolución y la vanguardia van de la mano: son el Zeitgeist o espíritu de la época, un contexto que es parte sustancial del proceso, y como lo calificarían los Smithson, un contexto heroico. El concepto se centra en la tríada Bauhaus: diseño + tecnología x economía. En cuanto al método, se fijan una serie de parámetros –las variables del experimento- que se agrupan en tres categorías distintas: topología, tipología y tecnología. La combinación de las variables de cada categoría dará lugar a un sistema con unas características determinadas: una definición del espacio, una forma, un lenguaje y una tecnología, características que permiten establecer las reglas para su desarrollo. Los sistemas resultantes son tres, denominados según su doble condición tipológica/ tecnológica: 1. Sistema de muro de carga: Viviendas adosadas en zig-zag o Mauerwerksbauten. 2. Sistema de esqueleto de acero: Viviendas aisladas o Stahlskelettbauten 3. Sistema de hormigón armado: Viviendas en hilera recta o Betonbauten Las villas Am Rupenhorn se plantean a continuación como verificación de este proceso: la síntesis de las categorías desarrolladas en la Colonia. Pero llegan en un momento de gracia, justo cuando los Luckhardt y Anker se encuentran profundamente implicados en el proceso de desarrollo de un nuevo lenguaje y con la reciente experiencia de la Colonia, que ha sido un éxito en casi todos los aspectos posibles. “En 1930, están en la cumbre”, como diría su mejor crítico y antiguo colaborador: Achim Wendschuh. En las Villas, los arquitectos integran su lenguaje, ya plenamente moderno, con sus experiencias previas: las que los relacionan con su reciente expresionismo (que se podría calificar como Kunstwollen) y con la tradición clásica de la cultura arquitectónica alemana: el sentido del material que deben a Semper y la sensibilidad hacia el paisaje, que toman de Schinkel. El extraordinario interés de las Villas se debe a factores como el tratamiento de la relación dual, poco habitual en la arquitectura moderna, la síntesis de lenguajes y las circunstancias de su momento histórico, factores que las han convertido en una propuesta única e irrepetible de una de las vías experimentales más interesantes y desconocidas de la Modernidad. ABSTRACT The experimental Housing Estate of Schorlemerallee and the Am Rupenhorn Villas are two projects completed by the brothers Wassili and Hans Luckhardt with Alfons Anker in Berlin in 1930. Both projects are part of the same process, starting with the Housing Estate --an exploration of the modern language in a series of phases- which culminates with the Villas project. The Villas Am Ruperhorn, designed immediately after the Housing development, are the synthesis and crowning point of this experience, even finally over passing it, since they have become a model of the house in nature, related with both the ideal of the classical villa and the new ways of life, reaching the condition of a modern canon. However, this is not its most important issue. The most remarkable condition is the project process itself -Housing versus Villas- a true experiment in concept, method and results, in which the authors research new technologies for new ways of living, developing an innovative language, with results in new prototypes, in the way Mies van der Rohe was looking for: “I have tried to make an architecture for a technological society. I have wanted to keep everything reasonable and clear… to have an architecture that anybody can do." The time and place could not be more favourable: Berlin from 1924 to 1930, in the very origin of Modern Movement. The experiment takes place with genuine scientific accuracy. Architects design, build and finance their own project, controlling all variables. Especially, and quite unusual, the control of the economic variable. Precisely the economic factor is for them a fundamental key to the process. It was shown to prove that the new architecture (or Neues Bauen, as they liked to call it) was able to build not only faster, better and more efficient dwellings for a new society, but also at lower cost. Revolution and Avant-garde use to move forward together, because they share the Zeitgeist --or time's spirit--, a context which is a substantial part of the process, and as the Alison & Peter Smithsons would describe, an heroic context. The concept focuses on the Bauhaus triad: Design + Technology x Economy. For the method, a number of variables are fixed --the experimental parameters-- that are later grouped into three distinct categories: Topology, Typology and Technology. The combination of these variables within each category gives way to several systems, with specific characteristics: a definition of space, a form, a language and a technology, thus allowing to establish the rules for its development: The resulting systems are three, called by double typological / technological issue: 1. Terraced Housing in zig-zag or Mauerwerksbauten (bearing wall system) 2. Detached Housing or Stahlskelettbauten (steel skeleton system) 3. Terraced Housing in one row or Betonbauten (reinforced concrete system) The Am Rupenhorn Villas are planned as the check of this process: the synthesis of the categories developed all through the Housing Estate research. The Am Ruperhorn project is developed in a crucial moment, just as the Luckhardts and Anker are deeply involved in the definition process of a new language after the recent experience of Schorlemerallee, which has been a success in almost all possible aspects. "In 1930, they are on the top” has said his best critic and long-time collaborator, Achim Wendschuh. In the Villas, the authors make up their fully modern language with their own background, related with their recent Expressionist trend (Kunstwollen) and with the classical tradition of the German architectural culture: the notion of material related with Semper and the sensible approach to the landscape, linked with Schinkel. Its extraordinary interest lay on diverse factors, such as dual relationships, unusual in modern architecture, synthesis of languages and circumstances of their historical moment, all factors that have become a unique and unrepeatable proposal in one of the most extraordinary experimental ways of Modernity.

An Electromechanical Coupled Model of the Probe of a Scanning Fiber Imaging System

Thesis (Master's)--University of Washington, 2016-06

Producing computer-generated tailored written information for stroke patients and their carers: System development and preliminary evaluation

Purpose: The aim of this project was to design and evaluate a system that would produce tailored information for stroke patients and their carers, customised according to their informational needs, and facilitate communication between the patient and, health professional. Method: A human factors development approach was used to develop a computer system, which dynamically compiles stroke education booklets for patients and carers. Patients and carers are able to select the topics about which they wish to receive information, the amount of information they want, and the font size of the printed booklet. The system is designed so that the health professional interacts with it, thereby providing opportunities for communication between the health professional and patient/carer at a number of points in time. Results: Preliminary evaluation of the system by health professionals, patients and carers was positive. A randomised controlled trial that examines the effect of the system on patient and carer outcomes is underway. (C) 2004 Elsevier Ireland Ltd. All rights reserved.

The promotion and disruption of community service delivery systems

In this paper, we report data drawn from a larger project on the functioning of the Queensland community service delivery system, particularly that providing services to people with disabilities. Our reasoning for focusing at this level is that, from the service user's perspective, support is derived from the service delivery system, not just individual service providers. Defining the service delivery system as formal services and informal support networks, we undertook interviews and focus groups with service providers in six areas in Queensland: inner urban, outer urban, rural and remote. The period on which we report is one in which considerable reform activity had been undertaken by funding bodies of the Commonwealth and State governments. We report on those factors we identified which promote the integrated functioning of the service delivery system, as well as those factors that disrupt it. We conclude with a brief evaluative analysis of the current status of the system.

Drug delivery strategies for the treatment of Helicobacter pylori infections

Helicobacter pylori is one of the most common pathogenic bacterial infections, colonising an estimated half of all humans. It is associated with the development of serious gastroduodenal disease - including peptic ulcers, gastric lymphoma and acute chronic gastritis. Current recommended regimes are not wholly effective and patient compliance, side-effects and bacterial resistance can be problematic. Drug delivery to the site of residence in the gastric mucosa may improve efficacy of the current and emerging treatments. Gastric retentive delivery systems potentially allow increased penetration of the mucus layer and therefore increased drug concentration at the site of action. Proposed gastric retentive systems for the enhancement of local drug delivery include floating systems, expandable or swellable systems and bioadhesive systems. Generally, problems with these formulations are lack of specificity, limited to mucus turnover or failure to persist in the stomach. Gastric mucoadhesive systems are hailed as a promising technology to address this issue, penetrating the mucus layer and prolonging activity at the mucus-epithelial interface. This review appraises gastroretentive delivery strategies specifically with regard to their application as a delivery system to target Helicobacter. As drug-resistant strains emerge, the development of a vaccine to eradicate and prevent reinfection is an attractive proposition. Proposed prophylactic and therapeutic vaccines have been delivered using a number of mucosal routes using viral and non-viral vectors. The delivery form, inclusion of adjuvants, and delivery regime will influence the immune response generated. © 2005 Bentham Science Publishers Ltd.

Reducible disulfide-based non-viral gene delivery systems

The design and synthesis of safe efficient non-viral vectors for gene delivery has attracted significant attention in recent years due primarily to the severe side-effect profile reported with the use of their viral counterparts. Previous experiments have revealed that the strong interaction between the carriers and nucleic acid may well hinder the release of the gene from the complex in the cytosol adversely affecting transfection efficiency. However, incorporating reducible disulfide bonds within the delivery systems themselves which are then cleaved in the glutathione-rich intracellular environment may help in solving this puzzle. This review focuses on recent development of these reducible carriers. The biological rationale and approaches to the synthesis of reducible vectors are discussed in detail. The in vitro and in vivo evaluations of reducible carriers are also summarized and it is evident that they offer a promising approach in non-viral gene delivery system design.

Development and formulation of wax-based transdermal drug delivery systems

Topical and transdermal formulations are promising platforms for the delivery of drugs. A unit dose topical or transdermal drug delivery system that optimises the solubility of drugs within the vehicle provides a novel dosage form for efficacious delivery that also offers a simple manufacture technique is desirable. This study used Witepsol® H15 wax as a abase for the delivery system. One aspect of this project involved determination of the solubility of ibuprofen, flurbiprofen and naproxen in the was using microscopy, Higuchi release kinetics, HyperDSC and mathematical modelling techniques. Correlations between the results obtained via these techniques were noted with additional merits such as provision of valuable information on drug release kinetics and possible interactions between the drug and excipients. A second aspect of this project involved the incorporation of additional excipients: Tween 20 (T), Carbopol®971 (C) and menthol (M) to the wax formulation. On in vitro permeation through porcine skin, the preferred formulations were: ibuprofen (5% w/w) within Witepsol®H15 + 1% w/w T; flurbiprofen (10% w/w) within Witepsol®H15 + 1% w/w T; naproxen (5% w/w) within Witepsol®H15 + 1% w/w T + 1% C and sodium diclofenac (10% w/w) within Witepsol®H15 + 1% w/w T + 1% w/w T + 1% w/w C + 5% w/w M. Unit dose transdermal tablets containing ibuprofen and diclofenac were produced with improved flux compared to marketed products; Voltarol Emugel® demonstrated flux of 1.68x10-3 cm/h compared to 123 x 10-3 cm/h for the optimised product as detailed above; Ibugel Forte® demonstrated a permeation coefficient value of 7.65 x 10-3 cm/h compared to 8.69 x 10-3 cm/h for the optimised product as described above.

Nanotechnology for the delivery of vaccines

Liposomes offer an ideal platform for the delivery of subunit vaccines, due to their versatility and flexibility, which allows for antigen as well as immunostimulatory lipids and TLR agonists to become associated with these bilayered vesicles. Liposomes have the ability to protect vaccine antigen, as well as enhance delivery to antigen presenting cells, whilst the importance of cationic surface charge for delivery of TB subunit vaccines and formation of an ‘antigen depot’ may play a key role in boosting cell-mediated immunity and Th1 immune responses. The rational design of vaccine adjuvants requires the thorough investigation into the physicochemical characteristics that dictate the function of a liposomal adjuvant. Within this thesis, physicochemical characteristics were investigated in order to show any effects on the biodistribution profiles and the ensuing immune responses of these formulations. Initially the role of liposome charge within the formulation was investigated and subsequently their efficacy as vaccine adjuvants in combination with their biodistribution was measured to allow the role of formulation in vaccine function to be considered. These results showed that cationic surface charge, in combination with high loading of H56 vaccine antigen through electrostatic binding, was crucial in the promotion of the ‘depot-effect’ at the injection site which increases the initiation of Th1 cell-mediated immune responses that are required to offer protection against tuberculosis. To further investigate this, different methods of liposome production were also investigated where antigen incorporation within the vesicles as well as surface adsorption were adopted. Using the dehydration-rehydration (DRV) method (where liposomes are freeze-dried in the presence of antigen to promote antigen encapsulation) and the double emulsion (DE) method, a range of liposomes entrapping antigen were formulated. Variation in the liposome preparation method can lead to antigen entrapment within the delivery system which has been shown to be greater for DRV-formulated liposomes compared to their DE-counterparts. This resulted in no significant effect on the vaccine biodistribution profile, as well as not significantly altering the efficacy of cationic liposomal adjuvants. To further enhance the efficacy of these systems, the addition of TLR agonists either at the vesicle surface as well as within the delivery system has been displayed through variation in the preparation method. Anionic liposomal adjuvants have been formulated, which displayed rapid drainage from the injection site to the draining lymph nodes and displayed a reduction in measured Th1 immune responses. However, variation in the preparation method can alter the immune response profile for anionic liposomal adjuvants with a bias in immune response to Th2 responses being noted. Through the use of high shear mixing and stepwise incorporation, the efficient loading of TLR agonist within liposomes has been shown. However, interestingly the conjugation between lipid and non-electrostatically bound TLR agonist, followed by insertion into the bilayer of DDA/TDB resulted in localised agonist retention at the injection site and further stimulation of the Th1 immune response at the SOI, spleen and draining lymphatics as well as enhanced antibody titres.

Characterization and optimization of bilosomes for oral vaccine delivery

Oral vaccines offer significant benefits due to the ease of administration, better patient compliance and non-invasive, needle-free administration. However, this route is marred by the harsh gastro intestinal environment which is detrimental to many vaccine formats. To address this, a range of delivery systems have been considered including bilosomes; these are bilayer vesicles constructed from non-ionic surfactants combined with the inclusion of bile salts which can stabilize the vesicles in the gastro intestinal tract by preventing membrane destabilization. The aim of this study was to investigate the effect of formulation parameters on bilosome carriers using Design of Experiments to select an appropriate formulation to assess in vivo. Bilosomes were constructed from monopalmitoylglycerol, cholesterol, dicetyl phosphate and sodium deoxycholate at different blends ratios. The optimized bilosome formulation was identified and the potential of this formulation as an oral vaccine delivery system were assessed in biodistribution and vaccine efficacy studies. Results showed that the larger bilosomes vesicles (~6 µm versus 2 µm in diameter) increased uptake within the Peyer's patches and were able to reduce median temperature differential change and promote a reduction in viral cell load in an influenza challenge study. © 2013 Informa UK, Ltd.

Information-Analytical System for Design of New Inorganic Compounds

The principles of design of information-analytical system (IAS) intended for design of new inorganic compounds are considered. IAS includes the integrated system of databases on properties of inorganic substances and materials, the system of the programs of pattern recognition, the knowledge base and managing program. IAS allows a prediction of inorganic compounds not yet synthesized and estimation of their some properties.