979 resultados para Citocinas Teses


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The Assistive Technology makes the lives of people without disabilities more practical, but for people with disabilities it can make things possible. For these individuals can provide the conditions, making their limitations are not obstacles to their social and personal empowerment, and may contribute to their effective participation in tasks that were previously impossible, such as remaining seated correctly. A segment on which the necessary assistive technology works, is the adaptation of furniture such as chairs, tables, chairs-to-wheels for individuals with disabilities, as well as cerebral palsy. The study aimed to conduct a survey and brief description of theses and dissertations produced in Brazil on furniture adapted for students with cerebral palsy. We made searching in databases of libraries of major universities and federal state national banks, theses and dissertations. Nine studies were selected for description. It was concluded that there is a considerable array of professionals, both in engineering as the field of rehabilitation, to contribute in the adaptation, creation and even the manufacture of furniture for children with cerebral palsy and that the securities most studied are the chairs and their components.

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Coordenao de Aperfeioamento de Pessoal de Nvel Superior (CAPES)

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Estudos tm demonstrado que o reconhecimento inicial de microrganismos mediado por receptores celulares expressos em clulas da imunidade inata denominados receptores de reconhecimento de padres (PRRs). Assim, a interao entre molculas de superfcie dos patgenos e receptores homlogos presentes na membrana celular de moncitos, modula a fagocitose, a ativao da clula e conseqentemente a produo de citocinas. Trabalhos tm demonstrado a importncia da estimulao de receptores toll-like 2 e 4 (TLR2 e TLR4), receptores de manose (MR) e dectina-1 de moncitos, tanto em infeces bacterianas como fngicas, culminando com induo de produo de vrias citocinas. A IL-18 uma citocina indutora de IFN-g e possui uma ao extremamente importante, por ser capaz de promover tanto uma resposta do tipo Th1 ou Th2, dependendo do contexto de estimulao e do microambiente de citocinas. Em trabalho recente demonstramos que a IL-18 possui uma ao importante sobre o aumento da expresso de MR em moncitos humanos, e o reconhecimento do Paracoccidioides brasiliensis via esse receptor causaria aumento do crescimento fngico no interior da clula. Dessa forma, os objetivos do presente projeto foram: 1) Avaliar a ao da IL- 18 sobre a expresso do receptor dectina-1 por moncitos humanos desafiados in vitro com diferentes cepas do P. brasiliensis; b) Avaliar a participao da dectina-1 na induo da produo de IL-18, TNF-a e IL-10 por moncitos desafiados in vitro com diferentes cepas do P. brasiliensis. Assim, moncitos de indivduos normais tratados in vitro com IL-18 foram desafiados com diferentes de cepas do P. brasiliensis, e a expresso do receptor dectina-1 foi avaliada pela tcnica de citometria de fluxo. A dosagem de IL-18, TNF-a e IL-10 no sobrenadante de cultura de moncitos desafiados com P. brasiliensis foi realizada utilizando a tcnica de ELISA . Os resultados mostraram que a ...

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Pre-eclampsia (PE) is a complication of human pregnancy characterized by hypertension and proteinuria after 20 weeks of gestation. Its incidence varies from 5% to 7% of pregnancies and is a major cause of morbidity and maternal and fetal mortality. This is a multisystemic disease, with focus on vascular dysfunction and is closely related to the exacerbated activation of the immune system. In addition to increased activation of monocytes and granulocytes, there is an elevated production of proinflammatory cytokines in pregnant women with PE. The nuclear transcription factor-kB (NF-kB) is present in the cells of the immune system and is responsible for transcription of genes related to inflammation. Whereas the PE is associated with intense inflammatory response, the use of substances modulating the activity of NF-kB factor could be useful in alleviating the inflammation present in these patients. Silibinin is the main component of silymarin, a polyphenolic extract obtained from fruits and seeds of Sylibum marianum with potent hepatoprotective, anti-inflammatory and anti-fibrotic activities. The silibinin mechanism of action includes the ability to inhibit NF-kB activation and, consequently, its migration to the nucleus. The objective of this study was to assess whether silibinin modulates the activity of NF-kB and the production of inflammatory cytokines in mononuclear cells of patients with PE. We evaluated 34 pregnant women with PE, 20 normotensive pregnant women (GN) and 15 non-pregnant women (NG). Peripheral blood mononuclear cells (PBMC) were obtained from those groups of women and cultured in the presence or absence of silibinin (5 uM or 50 uM) and stimulated or not with lipopolysaccharide (LPS) for 18 h to obtain supernatant for determination of tumor necrosis factor-alpha (TNF-) and interleukin-1 (IL-1) by enzyme immunoassay (ELISA). The cells were...(Complete abstract click electronic access below)

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A infeco genital por Papilomavrus Humano (HPV) uma das doenas sexualmente transmissveis (DST), de origem viral, mais prevalente no mundo. As leses intraepiteliais escamosas de baixo grau (LIEBG) e de alto grau (LIEAG), assim como carcinoma cervical invasor (CCI), esto associadas presena do HPV. A resposta imune tem papel importante na infeco pelo HPV na crvice uterina, sendo as citocinas importantes reguladoras da transcrio viral. Avaliar a concentrao de IL-1, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12, IFN- e TNF- no soro de mulheres portadoras de LIEBG, LIEAG e CCI. Foram includas no estudo 40 mulheres com diagnstico histopatolgico de LIEBG (n=11), LIEAG (n=10), CCI (n=10) e 9 mulheres com suspeita de doena HPV induzida, mas sem alteraes histopatolgicas na bipsia do colo do tero (controle), atendidas no Ambulatrio de Colposcopia da Faculdade de Medicina de Botucatu (UNESP) e no Ambulatrio de Ginecologia Preventiva do Hospital Amaral Carvalho, Ja, SP. O sangue perifrico foi colhido por puno venosa e o soro armazenado at o processamento. A dosagem das citocinas no soro foi avaliada por ensaio imunoenzimtico (ELISA). A pesquisa de HPV foi realizada empregando-se a tcnica da reao em cadeia da polimerase (PCR). Os dados scio-demogrficos foram obtidos por entrevista no momento da consulta ginecolgica. A mediana de idade das pacientes do grupo controle, LIEBG, LIEAG, e CCI foi de 38 (21-69), 32,5 (17-51), 39 (23-65) e 51,5 (29-72), respectivamente. Houve diferena estatisticamente significante de mediana de idade das pacientes do grupo CCI quando esta foi comparada com os demais grupos. Em relao s caractersticas das pacientes, 76% eram brancas, 68% relataram unio estvel, 31% concluram o 2 grau, 42% eram fumantes, 37% das mulheres relataram 3 ou mais parceiros sexuais durante... (Resumo completo, clicar acesso eletrnico abaixo)

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A quantificao de citocinas felinas possibilita uma avaliao do sistema imune do animal em diferentes doenas, permite tambm a identificao de diferentes fatores que alteram sua secreo, e colabora na compreenso da patognese das enfermidades. Em humanos e camundongos essa mensurao feita principalmente pelo mtodo de ELISA, mas para os felinos h uma menor disponibilidade de kits especficos para determinadas citocinas, e estes possuem um custo elevado. Assim, uma alternativa utilizar a reao de PCR em tempo real com transcrio reversa (RT-qPCR) para quantificao absoluta dos RNAs mensageiros das citocinas felinas, uma tcnica bastante sensvel e especfica para analisar a expresso desses genes. Com esse intuito, esse trabalho teve como objetivo clonar as sequncias gnicas codificantes de citocinas, para construir uma curva padro para a qPCR. Assim, foi feita extrao de RNA de amostras de sangue total de gatos domsticos, e estes foram tratados com DNAse para evitar contaminao por DNA genmico. O cDNA foi sintetizado, e amplificado com os primers especficos para cada fragmento codificante de citocina e o GAPDH felino. Cada amplicon purificado foi inserido em um plasmdeo comercial, e introduzido em bactrias E. coli cepa DH5. Os clones recombinantes foram sequenciados para confirmar a insero do fragmento no vetor. As curvas padro da qPCR foram construdas com cada plasmdeo recombinante numa de 106 a 101 cpias por reao. O sequenciamento mostrou que todos os clones eram semelhantes queles encontrados no GenBank. A eficincia das amplificaes, baseado no slope das curvas padro foram: 101,3% para GAPDH, 99,1% para IL-1, 83,2% para IL-6, 94,4% para IL-10, 105,5% para IL-12, 83,4% para IFN- e 83,8% para TNF-. O valor de r2 foi de 0,99 em todas as reaes. Dessa forma, com a clonagem dos fragmentos... (Resumo completo, clicar acesso eletrnico abaixo)

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Coordenao de Aperfeioamento de Pessoal de Nvel Superior (CAPES)

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Coordenao de Aperfeioamento de Pessoal de Nvel Superior (CAPES)

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Coordenao de Aperfeioamento de Pessoal de Nvel Superior (CAPES)

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Conselho Nacional de Desenvolvimento Cientfico e Tecnolgico (CNPq)

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This paper aims to base the thesis that the varied currents of the post-modern thought, in so far as have recourse to relativism to a greater or lesser degree, contribute to the legitimation of the religious education in public schools and, in a broader aspect, the religion in the public spaces. The methodological resource used to demonstrate the link between post-modern thought and religious education is the analysis at the historical and dialectical materialism perspective of publications produced to increase teachers on this discipline that is, in compliance with the Constitution, optional for students and mandatory for public elementary schools. The subject of this analysis is the learning content and has as prime concern to highlight the discourse of legitimation recurrent in authors when it is explicit in itself and explicit when it does not. The religious education in public schools as object of study allows us to understand the relationship between the post-modern thought, with its hegemony space within the human sciences and contextualized in the neoliberal economic environment, and the learning to learn pedagogies currently hegemonic in the educational landscape. We intend to contribute to the legal debate on laity, the philosophical debate on post-modernism and especially to the debate on the pedagogical theories in the educational fundaments.

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The present article aims to elaborate an objective analytical panel, initially from the 1920- 1930 when Luiz Igncio Romeiro de Anhaia Mello, cathedratic in the urban question, started to quote a series of authors and American plans as references to their reflections, mainly when the point was the verticalization or not at So Paulo City. From a broad bibliographic research with the systematization of the register from the debate between Anhaia Mello, this article intends to point out how the transposition of the urbanistic conceptions to the academic environment happened and, at the same time, the legal normatization of the So Paulo City that resulted in the creation of a draft of the regulations for use and occupation of the soil. The systematizations and analysis were based in a broad reading of the bibliography and documental sources that refer to the proposed theme. The books of the library of FAU-USP, specifically the one from SAGMACS Sociedade de Anlises Grficas e Mecanogrficas Aplicadas aos Complexos Sociais ( Society of Graphical Mechanical-Graphic Analysis Applied to Social Complexes) -, aiming to select papers and the collection of Anhaia Mellos family, so that a reconstruction of the intellectual and professional journey of the Urbanism Professor. The systematization of the collected data and posterior comparison with the bibliographical study was fundamental for the reconstruction of the reflection of Anhaia Mello about the ways of the urban streets and roads at Sao Paulo City should be lead to on the middle of the XX century having as a guideline the debate between Anhaia Mello very common on the 1950s for less important countries when dealing with the organization of the size of industrial metropolis. It was possible to identify the crystallization of one urbanistic proposal for the city: the from Anhaia Mello, that bet on the reversion of the metropolitan cycle by stopping the urban growth by the application of the theoretical conception of the garden city.