A cytochrome P450 terpenoid hydroxylase linked to the suppression of insect juvenile hormone synthesis

A cDNA encoding a cytochrome P450 enzyme was isolated from a cDNA library of the corpora allata (CA) from reproductively active Diploptera punctata cockroaches. This P450 from the endocrine glands that produce the insect juvenile hormone (JH) is most closely related to P450 proteins of family 4 and was named CYP4C7. The CYP4C7 gene is expressed selectively in the CA; its message could not be detected in the fat body, corpora cardiaca, or brain, but trace levels of expression were found in the midgut and caeca. The levels of CYP4C7 mRNA in the CA, measured by ribonuclease protection assays, were linked to the activity cycle of the glands. In adult females, CYP4C7 expression increased immediately after the peak of JH synthesis, reaching a maximum on day 7, just before oviposition. mRNA levels then declined after oviposition and during pregnancy. The CYP4C7 protein was produced in Escherichia coli as a C-terminal His-tagged recombinant protein. In a reconstituted system with insect NADPH cytochrome P450 reductase, cytochrome b5, and NADPH, the purified CYP4C7 metabolized (2E,6E)-farnesol to a more polar product that was identified by GC-MS and by NMR as (10E)-12-hydroxyfarnesol. CYP4C7 converted JH III to 12-trans-hydroxy JH III and metabolized other JH-like sesquiterpenoids as well. This ω-hydroxylation of sesquiterpenoids appears to be a metabolic pathway in the corpora allata that may play a role in the suppression of JH biosynthesis at the end of the gonotrophic cycle.

Absolute abundances of benthic foraminifers and stable isotopes from the Alvin dives 3709-3712

The presence of gas hydrates on the Blake Ridge diapir, northeastern Atlantic Ocean, offers an opportunity to study the impact of methane seepage on the ecology and geochemistry of benthic foraminifera in the late Holocene. Three push cores, covering a time span of ~ 1000 yrs, were retrieved from three distinct microhabitats at the top of the diapir at a water depth of ~ 2150 m: (i) sediments away from seepage (control core), (ii) sediments overlain by clusters of methanotrophic and thiotrophic bivalves, and (iii) chemoautotrophic microbial mats. The foraminiferal assemblages at the two seep sites are marked by a reduction in benthic foraminiferal species diversity, coupled with a near-absence of agglutinated species. However, an opportunistic population rise in CH4- or H2S-tolerant calcareous species (e.g., Globocassidulina subglobosa and Cassidulina laevigata) that utilize the abundant trophic resources at the seeps has led to an increase in the overall assemblage density there. The delta18O and delta13C values of three species of benthic foraminifera - Gyroidinoides laevigatus, Globocassidulina subglobosa, and Uvigerina peregrina - and the planktonic species Globorotalia menardii were acquired from all three cores. The benthic species from methane seeps yield delta13C values of 0.1 to - 4.2 (per mil VPDB), that are distinctly more 13C-depleted relative to the delta13C of 0.4 to - 1.0 (per mil VPDB) at the control (off seep) site. The species from a mussel-bed site exhibit more negative delta13C values than those from microbial mats, possibly reflecting different food sources and higher rate of anaerobic oxidation of methane. The positive delta13C values in the paired planktonic species suggest that authigenic carbonate precipitation did not overprint the observed 13C depletions. Hence the probable cause of negative delta13C of benthic foraminifera is primary calcification from Dissolved Inorganic Carbon (DIC) containing mixed carbon fractions from (a) highly 13C-depleted, microbially-oxidized methane and (b) a seawater source.

German American annals.

Title from caption.

A Allemanha (dias idos e vividos)

Prefacio.--A Europa depois da paz.--Aspectos do socialismo germanico.--Á jornada constitucional.--O problema das reparações.--A Alta Silesia.--O desarmamento.--Tirpitz, o eterno.--Harden.--Gustavo Killian.--Tres generaes.--A propaganda anti-germanica.--Gothein.--O Cesar loiro da Germania.--Haber.--O bloqueio e as suas consequencias.--Helfferich e Erzberger.--B. Dernberg.--Groener.--Falkenhayn, o fascinador.--Hindenburg.--Wassermann.--Kautsky e Ledebour.--Spa.--Theodor Wolff.--A rive gauche.--A. Müller.--Rathenau, o animador espiritual.--Ernst zu Reventlow e Stinnes.--Os Estados Unidos e a Europa.

De distributione geographica plantarum : secundum coeli temperiem /

La h. de lam. "Al. Humboldt del. Marchais perfl 1815, Coutant sculps L. Aubert scrip., De l'Imprimerie de langlois", representa "Geographiae plantarum lineamenta"

Nouveau système des blattaires /

Mode of access: Internet.

Schwenkfelder hymnology and the sources of the first Schwenkfelder hymn-book printed in America

Originally presented as the author's thesis (Ph. D)--University of Pennsylvania, 1906.

Walt Whitman and the Germans, a study

"Reprinted from German American annals, vol. IV."

Catalogus codicum manu scriptorum Bibliothecae regiae monacensis ...

Vol. 2 (not yet published) was reserved for Greek manuscripts. A catalog of the Greek manuscripts by I. Hardt was published in 5 vol. in 1806-12.

Quaedam ad anatomiam Blattae germaniscae perinentia.

Inaug.-diss.--Dorpat.