421 resultados para Bitter melon


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Two separate experiments using the Differential Decay Curve Method have been performed to extract mean lifetimes of excited states in 106Cd. The medium-spin states of interest were populated by the 98Mo( 12C, 4n) 106Cd reaction performed at the Wright Nuclear Structure Lab., Yale University. From this experiment, two isomeric state mean lifetimes have been deduced. The low-lying states were populated by the 96Mo(13C, 3n)106Cd reaction performed at the Institut für Kernphysik, Universität zu Köln. The mean lifetime of the Iπ = 21 + state was deduced, tentatively, as 16.4(9) ps. This value differs from the previously accepted literature value from Coulomb excitation of 10.43(9) ps.

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动物的适应进化是生物学研究的最基本的问题之一。虽然,人们早从形态学上研究了动物的适应现象,但是对适应的遗传学机制知之甚少。动物感觉系统对周围环境的适应是动物适应进化中的一个关键问题。因此,我们选取了动物感觉系统中苦味受体基因家族(T2R)和犁鼻器受体1基因家族(VIR)为研究对象,对哺乳动物适应性进化的分子机制进行探讨。通过生物信息学和分子生物学手段相结合,我们在哺乳动物6个目共16个物种中获得了157个的苦味味觉受体基因,并对这些TZR基因的系统发育关系进行分析,结果显示哺乳动物的TZR基因家族经历了"生一和一灭"的进化模式,即频繁的基因重复和假基因化。另外,结果还显示这些基因可以被分为3个主要类群,分别命名为A,B和C。有趣的是,B和C类群的基因在所研究的物种间普遍是一对一的直系同源的,而A类群基因则显示了种属或世系特异性。有可能B和C类群的基因是识别哺乳动物共同的苦味物质所必需的,而A类群基因则是用于识别具有种属特异性的苦味物质。这个分析还揭示了在系统发育关系上近相关的基因在它们的染色体位置上也是靠近的,这证明了串联重复是新的TZR基因产生的主要方式。此外,通过核昔酸的异义替换数和同义替换数的比较显示不同物种新近产生的基因所编码的受体蛋白在膜外区的异义替换数显著地大于同义替换数,提示着这些通过基因重复产生的新基因受到了正选择的作用。在自然界中,许多的天然有毒物质一般都是苦的,因此,我们推测哺乳动物TZR基因的分"化选择是为了使其在探索新的生活环境和寻找新食物时能够辨别出更多不同的有毒物质,更好地适应新环境。此外,我们还研究了苦味受体基因和甜味/鲜味受体基因的进化途径。结果显示苦味受体基因和甜味/鲜味受体基因在进化上具有远相关,并具有不同的进化途径,提示着这可能是导致了这些受体基因具有不同功能,传导不同味觉的原因。犁鼻受体噬因家族(VIR)是哺乳动物的信息素受体。应用生物信息学手,我们从大鼠和小鼠的基因组中分别得到了152和115个VIR基因。大鼠VIR基因家族包含11个亚家族,其中10个是与小鼠共享的,而M家族是大鼠特有的;另夕卜大鼠缺少了H和I亚家族,而这两个亚家族存在于小鼠的基因组中。系统发育关系分析发现,"生一和一灭"进化模式也在V1R基因的进化过程中占了主导地位。所有检测到的亚家族都出现于啮齿目和灵长目分歧之后,这说明V1R基因的多样性反映了这一基因家族在啮齿目内基因重复、丢失,基因漂变及自然选择等作用的动态过程。我们的分析还表明大部分不同亚家族下的基因簇爆发的时间接近于大、小鼠分歧的时间。此外,用最大似然法分析的结果表明在这些基因簇中异义替换和同义替换的比值远大于1,揭示了正选择在这些基因的分化过程的作用。一般认为V1R在动物识别信息素过程中起重要的作用,因此我们推测V1R基因的适应性进化是为了使不同的哺乳动物能够识别不同的、复杂的信息素。

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本文主要研究了泸州老窖古酿酒作坊内外环境空气真菌和空气细菌的群落结构和分布特征。结果如下: 作坊内外环境空气微生物浓度差别显著,并随季节变换而变化,春、夏季微生物浓度较高,秋、冬季较低,空气真菌在夏季达到最高,细菌在春季最高。 古作坊内外环境检测到的真菌均为16 属,但优势菌属不同,作坊外的优势菌属为青霉属(Penicillium)、曲霉属(Aspergillus)、无孢菌(non-sporing)、枝孢霉属(Cladosporium)和链格孢属(Alternaria);而作坊内优势菌属为曲霉属、青霉属、酵母菌(Yeast)、无孢菌,作坊内还含有较高浓度的根霉属(Rhizopus)、毛霉属(Mucor)、短梗霉属(Aureobasidiu),枝孢霉属和链格孢属等,曲霉属、酵母菌、根霉属、毛霉属为古酿酒作坊重要的酿酒真菌,青霉属、链格孢属为酿酒不利菌群。对古作坊内曲霉属进行了初步鉴定,主要是小冠曲霉(A.cristatellus)、米曲霉(A.oryzae)、黑曲霉(A.niger)和白曲霉(A.cadidus)。 空气细菌10 属21 种,作坊内外环境的优势菌属均为芽孢杆菌属(Bacillus)、微球菌属(Micrococcus)、葡萄球菌属(Staphylococcus)、假单胞菌属(Pseudomonad),其中芽孢杆菌属在作坊内占有绝对的优势,浓度比在40℅以上,是古酿酒作坊重要的酿酒细菌,另外还检测到较高浓度的乳酸杆菌(lactobucillus),这类菌容易使酒味发涩发苦,为酿酒不利菌。 作坊内外环境空气微生物表现出明显的交流现象。作坊内,青霉属、枝孢霉属、链格孢属、葡萄球菌属等杂菌占有一定比例;而在作坊外,芽孢杆菌属、曲霉属、根霉属(Rhizopus)、酵母菌等处于相对较高水平,绿化环境较好的营沟头作坊内的短梗霉属,枝孢霉属和链格孢属等杂菌含量低于什字头和新街子作坊。 The community structure and distribution characteristic of airborne microbes was investigated in ancient brewage workshops of luzhoulaojiao. The results are as follows: The concentration of airborne microbes was different in interior and exterior environment of ancient workshops, and also varied by seasons. microbial concentration was higher in spring and summer, and lower in fall and winner. The highest levels of airborne bacteria was in spring, but the fungal’s in summer. The identified genus of fungi were 16 in interior and exterior environment of the ancient workshops. But the dominant genus were different , The advantage genus in the interior were Aspergillus, Yeasts, Penicillum and Nonsporing and in the exterior were Penicillum, Nonsporing, Cladosporium, Aspergillus and Aureobasidiu. Rhizopus ,mucor, Aureobasidiu, Cladosporium, Alternaria and all also were at a higher level. Among these, Aspergillus, Yeasts, Rhizopus ,mucor are important vintage flora . Penicillum, Alternaria do harm to vintage. Aspergillus of ancient workshops was identified , the preponderant aspergillus species were A.cristatellus, A.oryzae, A.niger and A.cadidus in ancient brewage workshops. 10 genus 21 species bacteria were identified, the advantage genuses among the interior and exterior of the three workshops were bacillus, microccus, Staphylococcus Pseudomonas. Bacillus, which account for beyond 40℅ of the total bacteria concentration in all sampling pots, was the most dominant genus. Lactobacillus was identified at a high level in ancient workshops, it makes spirit taste bitter and astringent. So it is not a kind of good bacterium for vintage. The fungus in the interior and exterior atmosphere characterized intercommunion phenomenon. Obviously, the concentration of profitless fungus such as Penicillum, Cladosporium, Alternaria appeared in the interior, and the fungus such as Bacillus, Aspergillus, Rhizopus and Yeasts in the exterior were at a relatively high level. the harmfull fungus in yinggoutou workshops such as Aureobasidiu, Cladosporium, Alternaria and all were lower than shenzitou and xinjiezi workshops.

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O objetivo desta Circular Técnica é descrever as principais viroses que afetam espécies de cucrbitáceas no Brasil, quanto aos sintomas, etiologia, epidemiologia e medidas de controle.

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Visa levantar dados de custo de produção da aquicultura, a fim de subsidiar a criação de políticas públicas para o setor e auxiliar os produtores no gerenciamento de seus empreendimentos aquícolas.

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Comportamento do mercado de varejo nacional. Evolução dos preços. A abertura do mercado brasileiro à tilápia chinesa. O setor externo.

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Increased plasmin and plasminogen levels and elevated somatic cell counts (SCC) and polymorphonuclear leucocyte levels (PMN) were evident in late lactation milk. Compositional changes in these milks were associated with increased SCC. The quality of late lactation milks was related to nutritional status of herds, with milks from herds on a high plane of nutrition having composition and clotting properties similar to, or superior to, early-mid lactation milks. Nutritionally-deficient cows had elevated numbers of polymorphonuclear leucocytes (PMNs) in their milk, elevated plasmin levels and increased overall proteolytic activity. The dominant effect of plasmin on proteolysis in milks of low SCC was established. When present in elevated numbers, somatic cells and PMNs in particular had a more significant influence on the proteolysis of both raw and pasteurised milks than plasmin. PMN protease action on the caseins showed proteolysis products of two specific enzymes, cathepsin B and elastase, which were also shown in high SCC milk. Crude extracts of somatic cells had a high specificity on αs1-casein. Cheeses made from late lactation milks had increased breakdown of αs1-casein, suggestive of the action of somatic cell proteinases, which may be linked to textural defects in cheese. Late lactation cheeses also showed decreased production of small peptides and amino acids, the reason for which is unknown. Plasmin, which is elevated in activity in late lactation milk, accelerated the ripening of Gouda-type cheese, but was not associated with defects of texture or flavour. The retention of somatic cell enzymes in cheese curd was confirmed, and a potential role in production of bitter peptides identified. Cheeses made from milks containing high levels of PMNs had accelerated αs1-casein breakdown relative to cheeses made from low PMN milk of the same total SCC, consistent with the demonstrated action of PMN proteinases. The two types of cheese were determined significantly different by blind triangle testing.

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El estudio sobre las condiciones de fertilidad y variables de calidad del melón resultan de suma importancia para el mejoramiento de la aptitud y competitividad del producto. El objetivo de esta tesis fue determinar la influencia del contenido de potasio y su efecto sobre relaciones nitrógeno/potasio de la solución de fertilizante sobre la producción, calidad y sanidad de los frutos en dos cultivares de melén (tipo Honey Dew y Cantaloupe) bajo invernáculo y sobre propiedades físico químicas del suelo. Se determinaron propiedades de calidad, sanitarias y se analizaron propiedades relacionadas a la fertilidad de los suelos. El rendimiento y las variables de calidad como: diámetro ecuatorial y polar, sólidos totales y azúcares presentaron en ambos cultivares mayor valor en el tratamiento con mayor contenido de potasio. El grosor de la cáscara no mostró diferencias significativas, al igual que en la resistencia a la presión, como tampoco el color superficial, la longitud de la cavidad central ni el contenido de nitratos en los frutos. Ambos cultivares fueron susceptibles a la inoculación con el Fusarium semitectum, aunque Honey Dew presentó una mayor resistencia a la infección, pero sin diferencias significativas. La mayoría de las variables relacionadas al suelo no se modificaron con los tratamientos de fertilización con excepción del contenido de potasio en cosecha y poscosecha. Este hecho sumado a las relaciones significativas y positivas entre los contenidos de potasio del suelo y variables relacionadas a la calidad y producción del cultivo de melón, sugiere que el efecto de la fertilización se dio fundamentalmente a través de cambios en la disponibilidad de potasio.

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El estudio sobre las condiciones de fertilidad y variables de calidad del melón resultan de suma importancia para el mejoramiento de la aptitud y competitividad del producto. El objetivo de esta tesis fue determinar la influencia del contenido de potasio y su efecto sobre relaciones nitrógeno/potasio de la solución de fertilizante sobre la producción, calidad y sanidad de los frutos en dos cultivares de melén (tipo Honey Dew y Cantaloupe)bajo invernáculo y sobre propiedades físico químicas del suelo. Se determinaron propiedades de calidad, sanitarias y se analizaron propiedades relacionadas a la fertilidad de los suelos. El rendimiento y las variables de calidad como: diámetro ecuatorial y polar, sólidos totales y azúcares presentaron en ambos cultivares mayor valor en el tratamiento con mayor contenido de potasio. El grosor de la cáscara no mostró diferencias significativas, al igual que en la resistencia a la presión, como tampoco el color superficial, la longitud de la cavidad central ni el contenido de nitratos en los frutos. Ambos cultivares fueron susceptibles a la inoculación con el Fusarium semitectum, aunque Honey Dew presentó una mayor resistencia a la infección, pero sin diferencias significativas. La mayoría de las variables relacionadas al suelo no se modificaron con los tratamientos de fertilización con excepción del contenido de potasio en cosecha y poscosecha. Este hecho sumado a las relaciones significativas y positivas entre los contenidos de potasio del suelo y variables relacionadas a la calidad y producción del cultivo de melón, sugiere que el efecto de la fertilización se dio fundamentalmente a través de cambios en la disponibilidad de potasio.

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In 1900, Ernst Däumig (1866–1922) wrote to Karl Kautsky of his ‘bitter experiences’ of violence in the French Foreign Legion and then in the Prussian military that had led to his recent ‘conversion’ to the socialist worldview. This article takes up Däumig's letters, articles and travelogues, and a drama, to explore how he recast his experiences of colonial and military violence twice, first as a writer for a German soldiers’ journal and then as an aspiring socialist journalist. As well as a burden that pushed him to critique his past, Däumig's military and colonial experiences are shown to have been his starting capital in the world of journalism and politics. The article gives particular attention to the process of conversion, through which Däumig forged a new life narrative out of the moral tales offered by the adopted worldview and the events of his own past. In addition to providing a case study of worldview conversion, this article demonstrates how biographical research can challenge assumptions about the impact of colonial violence on German metropolitan culture. At the same time, this biographical analysis sheds light on the early career of one of the key figures in the German Revolution of 1918 to 1921. As co-chairman of the Independent Social Democratic Party (USPD), Däumig led the USPD into union with the Communist Party in 1920.

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We examined the role of common genetic variation in schizophrenia in a genome-wide association study of substantial size: a stage 1 discovery sample of 21,856 individuals of European ancestry and a stage 2 replication sample of 29,839 independent subjects. The combined stage 1 and 2 analysis yielded genome-wide significant associations with schizophrenia for seven loci, five of which are new (1p21.3, 2q32.3, 8p23.2, 8q21.3 and 10q24.32-q24.33) and two of which have been previously implicated (6p21.32-p22.1 and 18q21.2). The strongest new finding (P = 1.6 x 10(-11)) was with rs1625579 within an intron of a putative primary transcript for MIR137 (microRNA 137), a known regulator of neuronal development. Four other schizophrenia loci achieving genome-wide significance contain predicted targets of MIR137, suggesting MIR137-mediated dysregulation as a previously unknown etiologic mechanism in schizophrenia. In a joint analysis with a bipolar disorder sample (16,374 affected individuals and 14,044 controls), three loci reached genome-wide significance: CACNA1C (rs4765905, P = 7.0 x 10(-9)), ANK3 (rs10994359, P = 2.5 x 10(-8)) and the ITIH3-ITIH4 region (rs2239547, P = 7.8 x 10(-9)).

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Plant pathogens are a serious problem for seed export, plant disease control and plant quarantine. Rapid and accurate screening tests are urgently required to protect and prevent plant diseases spreading worldwide. A novel multiplex detection method was developed based on microsphere immunoassays to simultaneously detect four important plant pathogens: a fruit blotch bacterium Acidovorax avenae subsp. citrulli (Aac), chilli vein-banding mottle virus (CVbMV, potyvirus), watermelon silver mottle virus (WSMoV, tospovirus serogroup IV) and melon yellow spot virus (MYSV, tospovirus). An antibody for each plant pathogen was linked on a fluorescence-coded magnetic microsphere set which was used to capture corresponding pathogen. The presence of pathogens was detected by R-phycoerythrin (RPE)-labeled antibodies specific to the pathogens. The assay conditions were optimized by identifying appropriate antibody pairs, blocking buffer, concentration of RPE-labeled antibodies and assay time. Once conditions were optimized, the assay was able to detect all four plant pathogens precisely and accurately with substantially higher sensitivity than enzyme-linked immunosorbent assay (ELISA) when spiked in buffer and in healthy watermelon leaf extract. The assay time of the microsphere immunoassay (1 hour) was much shorter than that of ELISA (4 hours). This system was also shown to be capable of detecting the pathogens in naturally infected plant samples and is a major advancement in plant pathogen detection. © 2013 Charlermroj et al.

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The review essay opens with positive attributes of Ireland but then considers that the island has been subject to centuries of bitter dispute and unrest. The historical background to this is outlined, particularly the interactions between Ireland and its neighbouring island, Great Britain, which dominated Irish affairs. One policy adopted by the British was to encourage migration of Protestants into the largely Catholic island in the vain hope that this would reduce unrest. The
two islands were then united from 1801 as the United Kingdom of Great Britain and Ireland but demands from indigenous Irish Catholics for independence continued, resisted by the Protestant minority who wished to remain inside the UK. After the Great War a solution was imposed that granted most of Ireland independence but left the largely Protestant northeast corner within the UK as Northern Ireland. Reaction to and life with the Irish border are considered and the paper concludes with musings about its future.

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In the preparation of silica-supported nickel oxide from nickel nitrate impregnation and drying, the replacement of the traditional air calcination step by a thermal treatment in 1% NO/Ar prevents agglomeration, resulting in highly dispersed NiO. The mechanism by which NO prevents agglomeration was investigated by using combined in situ diffuse reflectance infrared fourier transform (DRIFT) spectroscopy and mass spectrometry (MS). After impregnation and drying, a supported nickel hydroxynitrate phase with composition Ni(3)(NO(3))(2)(OH)(4) had been formed. Comparison of the evolution of the decomposition gases during the thermal decomposition of Ni(3)(NO(3))(2)(OH)(4) in labeled and unlabeled NO and O(2) revealed that NO scavenges oxygen radicals, forming NO(2). The DRIFT spectra revealed that the surface speciation evolved differently in the presence of NO as compared with in O(2) or Ar. It is proposed that oxygen scavenging by NO depletes the Ni(3)(NO(3))(2)(OH)(4) phase of nitrate groups, creating nucleation sites for the formation of NiO, which leads to very small (similar to 4 nm) NiO particles and prevents agglomeration.