Efeitos da interação e toxicidade das proteínas Cry1 e Vip3A de Bacillus thuringiensis em Diatraea saccharalis (Fabr., 1794) (Lepidoptera: Crambidae)

Pós-graduação em Agronomia (Genética e Melhoramento de Plantas) - FCAV

Toxicidade e efeitos subletais de toxinas Cry de Bacillus thuringiensis Berliner em diferentes populações de Plutella xylostella (L.) (Lepidoptera: Plutellidae) em laboratório

Pós-graduação em Agronomia (Entomologia Agrícola) - FCAV

Effect of bacillus thuringiensis on the biological parameters and phytophagy of podisus nigrispinus (hemiptera: pentatomidae)

The effect of Bacillus thuringiensis Berliner 1911 var aizawai strain GC-91 (Bta) on the biological parameters and phytophagy of Podisus nigrispinus (Dallas 1851) (Hemiptera: Pentatomidae) were evaluated using the follow treatments: a) provision of deionized water and prey Plutella xylostella (Linnaeus 1758) (Lepidoptera: Plutellidae); b) provision of only a solution containing Bta; and c) provision of prey and the solution containing Bta. To evaluate the phytophagy of the predator, leaves of Brassica oleraceae var acephala Linnaeus cv Manteiga da Georgia were provided and replaced every two days, and subsequently stained by immersion in 1% acid fuchsin. Staining enabled the visualization of the feeding sheath, which allowed for the quantification of punctures inflicted by P nigrispinus. The phytophagy, reproductive capacity and biological cycle in P nigrispinus were negatively affected by the presence of Bta; however, its predatory capacity was not altered.

Comprehensive Analysis of Gene Expression Profiles of the Beet Armyworm Spodoptera exigua Larvae Challenged with Bacillus thuringiensis Vip3Aa Toxin

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Caracterização do gene chi_l555 e propriedades bioquímicas da quitinase de Bacillus thuringiensis

Pós-graduação em Agronomia (Genética e Melhoramento de Plantas) - FCAV

Compatibilidade entre produtos químicos e biológicos à base de Bacillus thuringiensis Berliner no controle de Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae)

Pós-graduação em Agronomia (Entomologia Agrícola) - FCAV

Synergism and Antagonism between Bacillus thuringiensis Vip3A and Cry1 Proteins in Heliothis virescens, Diatraea saccharalis and Spodoptera frugiperda

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Atividade imunomoduladora de parasporina de Bacillus thuringiensis sobre linfócitos e monócitos humanos

Bacillus thuringiensis is an environmental bacteria that produces a group of crystallizable proteins (Cry) that are toxic for several insects and worms species. Recently, it was described a novel class of Cry proteins called parasporins (PS) that showed cytotoxic effects on animal and human tumor cells. Six types of PS have been described so far, PS1 to PS6, and their cytotoxic activity has been studied. However, the direct effect on tumor cells has been the current research focus, while the immunomodulatory role of the PS has not been studied yet. Therefore, this study aimed to verify whether PS of TC 2.3.1R6 B. thuringiensis strain has immunostimulatory activity on human lymphocytes and monocytes. We have evaluated the protein toxicity against human cells, the lymphoproliferative activity and the effects on peripheral blood monocytes. The PS-PK showed no toxic or stimulating activity on lymphocyte proliferation. However, it inhibited the spontaneous production of IL-10 as well as ConA-induced and the production of IFN-γ. PS-PK decreased the release of hydrogen peroxide and increased the production of TNF- α by monocytes. PS-PK performed inhibitory production of hydrogen peroxide and TNF-α by monocytes, whereas PS-Tp showed stimulation of the production of hydrogen and TNF-α

Interação de proteínas Vip3A e Cry1la10 de Bacillus thuringiensis com atividade inseticida a lepidópteros-praga

Pós-graduação em Agronomia (Genética e Melhoramento de Plantas) - FCAV

Toxicidade e interação de proteínas Cry1 de Bacillus thuringiensis em Helicoverpa armigera (Hübner) (Lepidóptera: Noctuidae)

Pós-graduação em Agronomia (Genética e Melhoramento de Plantas) - FCAV

Mortalidade e efeitos subletais de Bacillus thuringiensis Berliner em Spodoptera albula (Walker, 1857)

Pós-graduação em Agronomia (Entomologia Agrícola) - FCAV

Isolation of Bacillus thuringiensis from the state of Amazonas, in Brazil, and screening against Aedes aegypti (Diptera, Culicidae)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Binding analysis of Bacillus thuringiensis Cry1 proteins in the sugarcane borer, Diatraea saccharalis (Lepidoptera: Crambidae)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Interactions of Bacillus thuringiensis bioinsecticides and the predatory stink bug Podisus nigrispinus to control Plutella xylostella

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

The structure and organization within the membrane of the helices composing the pore-forming domain of Bacillus thuringiensis δ-endotoxin are consistent with an “umbrella-like” structure of the pore

The aim of this study was to elucidate the mechanism of membrane insertion and the structural organization of pores formed by Bacillus thuringiensis δ-endotoxin. We determined the relative affinities for membranes of peptides corresponding to the seven helices that compose the toxin pore-forming domain, their modes of membrane interaction, their structures within membranes, and their orientations relative to the membrane normal. In addition, we used resonance energy transfer measurements of all possible combinatorial pairs of membrane-bound helices to map the network of interactions between helices in their membrane-bound state. The interaction of the helices with the bilayer membrane was also probed by a Monte Carlo simulation protocol to determine lowest-energy orientations. Our results are consistent with a situation in which helices α4 and α5 insert into the membrane as a helical hairpin in an antiparallel manner, while the other helices lie on the membrane surface like the ribs of an umbrella (the “umbrella model”). Our results also support the suggestion that α7 may serve as a binding sensor to initiate the structural rearrangement of the pore-forming domain.