An Allele of the Ripening-Specific 1-Aminocyclopropane-1-Carboxylic Acid Synthase Gene (ACS1) in Apple Fruit with a Long Storage Life1

An allele of the 1-aminocyclopropane-1-carboxylic acid (ACC) synthase gene (Md-ACS1), the transcript and translated product of which have been identified in ripening apples (Malus domestica), was isolated from a genomic library of the apple cultivar, Golden Delicious. The predicted coding region of this allele (ACS1-2) showed that seven nucleotide substitutions in the corresponding region of ACS1-1 resulted in just one amino acid transition. A 162-bp sequence characterized as a short interspersed repetitive element retrotransposon was inserted in the 5′-flanking region of ACS1-2 corresponding to position −781 in ACS1-1. The XhoI site located near the 3′ end of the predicted coding region of ACS1-2 was absent from the reverse transcriptase-polymerase chain reaction product, revealing that exclusive transcription from ACS1-1 occurs during ripening of cv Golden Delicious fruit. DNA gel-blot and polymerase chain reaction analyses of genomic DNAs showed clearly that apple cultivars were either heterozygous for ACS1-1 and ACS1-2 or homozygous for each type. RNA gel-blot analysis of the ACS1-2 homozygous Fuji apple, which produces little ethylene and has a long storage life, demonstrated that the level of transcription from ACS1-2 during the ripening stage was very low.

Consumer preferences for selected frozen concentrated apple juice.

Mode of access: Internet.

The apple situation in New York State, a preliminary report

Cover title.

Orchard practice for the control of blister canker of apple trees /

Cover title.

The fruiting habit of the apple tree.

Thesis (M. S. in Agr.) - Cornell Univ., June 1918.

Insect and mite pests of apple in California /

Mode of access: Internet.

Apple loss adjustment standards handbook.

Mode of access: Internet.

Preliminary benefit analysis of Endrin use on apple orchards /

Bibliography: leaves [86]-91.

Recent developments in the production and marketing of apple juice and cider

Cover title.

Apple alarm : public concern about pesticide residues in fruits and vegetables /

"March 10, 1989."

The golden apple; a play for Kiltartan children,

Mode of access: Internet.

Rose leaf and apple leaf /

"Seven hundred and fifty copies of this book have been printed on Van Gelder hand-made paper and the type distributed."