1000 resultados para 28-267


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现在全国上下深切关注着黄河的重大问题及其对策。其实问题的根源都出自人们对黄河流域尤其黄土高原自然资源的掠夺式开发 ,形成了“3个恶性循环”:广种薄收 ,薄收更广种 ,虽是罪魁祸首 ,但常反被忽视 ,致使生态环境脆弱 ,人民长期贫困 ;只有下游河床越淤越高 ,防洪大堤越筑越高 ,越高越险和断流历时越来越长 ,上溯速度越来越快 2个恶性循环的危害才使人惊恐。不难看出 ,这是恰与形成黄土高原自然规律完全相悖的人为地质过程的结果。可惜它还未引起人们深入充分的认识 ,难怪一向短缺一个为各家合力共识的治本对策。当今国家将经济发展的重点向中、西部转移 ,并要求重建一个山川秀美的大西北 ,黄土高原的持续开发与治理对策就显得更为举足轻重。由此其各项对策都将面临着转变观念、调整思路、实事求是地进行科学分析和抉择。笔者最近从陆地生态的发生发展及其整个地质历史演变过程的研究中发现 :“土壤水库”的发生发展及其演变是陆地生态发生发展的关键和“动力”,只要维护土壤水库的正常发展就能更好地保卫生态环境。黄土高原地区由于得天独厚的降尘堆积环境条件和持续的成壤过程 ,可使降水具有直接渗入“地下水库”的特殊功能。只要维护住高入渗土壤水库的存在就...

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三江源区不仅是地处青藏高原的全球气候变化的敏感区,也是我国甚至亚洲最重要河流的上游关键源区。作为提供物质基础的植被净初级生产力(Netprimaryproduction,NPP),是评价生态系统状况的重要指标。该文应用已在碳通量观测塔验证,扩展到区域水平的遥感-过程耦合模型GLOPEM-CEVSA,以空间插值的气象数据和1km分辨率的AVHRR遥感反演的FPAR数据为模型主要输入,模拟并分析了1988~2004年该区NPP时空格局及其控制机制。结果表明,该区植被平均NPP为143.17gC·m–2·a–1,呈自东南向西北逐渐降低的空间格局,其中,以森林NPP最高(267.90gC·m–2·a–1),其次为农田(222.94gC·m–2·a–1)、草地(160.90gC·m–2·a–1)和湿地(161.36gC·m–2·a–1),荒漠最低(36.13gC·m–2·a–1)。其年际变化趋势在空间上呈现出明显的差异,西部地区NPP表现为增加趋势,每10a增加7.8~28.8gC·m–2;而中、东部表现为降低趋势,每10a降低13.1~42.8gC·m–2。根据显著性检验,NPP呈增加趋势(趋势斜率b>0),显著性水平高于99%和95%的区域占研究区总面积的13.43%和20.34%,主要分布在西部地区;NPP呈降低趋势(趋势斜率b<0),显著性水平高于99%和95%的区域占研究区面积的0.75%和3.77%,主要分布在中、东部地区,尤以该区长江和黄河等沿线区分布更为集中,变化显著性也更高。三江源NPP的年际变化趋势的气候驱动力分析表明,整个区域水平上该地区植被生产力受气候变化的主导,西部地区暖湿化趋势,造成了该地区生产力较为明显的、大范围的增加趋势;但东、中部地区则主要受人类活动的影响,特别是长江、黄河等河流沿线,是人类居住活动密集的地区,造成这些地区放牧压力较大、草地退化严重,而该地区暖干化趋势加剧了这一过程。

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A novel 3D supramolecular assembly constructed from decavanadate and caffeine building blocks, (NH4)(2)(C8H10N4O2)(4)[H4V10O28].2H(2)O (1), has been synthesized in aqueous solution and characterized by elemental analysis, IR, H-1 NMR, V-51 NMR, TG-DTA, and single crystal X-Ray diffraction. The compound 1 crystallizes in monoclinic system, space group P2(1)/n, a = 15.801(1) Angstrom, b = 12.914(1) Angstrom, c = 15.913(2) Angstrom, beta = 113.55degrees, V = 2976.4 (5) Angstrom(3), Z = 2, R = 0.0498 with 6818 reflections. Water molecules, ammonium ions, and caffeine act as "cement" linking the polyanions into 1D chain along the c-axis by hydrogen bonding. In compound 1, extensive hydrogen-bond contacts and strong pi-pi interactions lead to an ordered 3D supramolecular framework. TG-DTA curves indicate that the weight loss of the complex can be divided into three stages.

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Novel soluble rare earth aromatic carboxylates were prepared. The triplet energy level of organic ligand was measured. The photoluminescence properties of the Tb3+ and EU3+ aromatic carboxylates and lifetimes were investigated, which indicated that these rare earth complexes have high quantum efficiency. Because of their excellent solubility, polymer-doping rare earth carboxylates were fabricated as thin Films by spin-coating method and their luminescence properties were studied. Some rare earth organic light-emitting diodes were successfully fabricated which performed high pure color. The maximum luminance of the device of ITO/PVK/PVK :Th (AS)(3)Phen: PBD/PBD/Al is 32 cd(.)m(-2) at 28 V.

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在真空电弧炉内制备了LaNi<,2>和LaNi<,2.28>合金,在超高压下处理LaNi<,2>合金,得到了具有Laves相结构的AB<,2>型金属间化合物,采用机械合金化装置对上述铸造合金进行了球磨处理,制备了LaNi<,2.28>非晶合金粉末.利用这些合金制备储氢电极,与烧结镍阳极组成单电池,研究各种电极的充放电性能,得到如下结论:(1)LaNi<,2>和LaNi<,2.28>铸态合金电极的放电容量分别为199.4mAh/g和241.0mAh/g;(2)铸态AB<,2>合金的循环性能和放电容量高于Laves相结构的AB<,2>型合金;(3)非晶化的合金电极非常容易被活化,但循环性能和放电容量都远低于铸态合金电极.

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系统地研究了2-乙基己氧基甲基膦酸二(2-乙基己基)酯(代号PT28)对硫酸、磷酸、硝酸、高氯酸、盐酸和醋酸六种酸的萃取行为,发现PT-28对硫酸、磷酸、高氯酸和盐酸均不萃取,只对硝酸和醋酸萃取,这与中性磷氧萃取剂甲基膦酸二甲庚酯(P350)和磷酸三正丁酯(TBP)对强酸的萃取有显著的不同,并对其萃取机理进行了讨论。

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对 Ni6 9.5Al2 8Y2 .5粉末进行球磨 ,获得 Ni Al金属间化合物 .研究粉末结构与球磨时间的关系 ,探索以中间合金的形式加入稀土元素的机械合金化方法 .结果表明 ,加入稀土元素后 ,用机械合金化的方法可以生成新的金属间化合物.

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系统研究了2-乙基已氧基甲基膦酸工(2-乙基已基)酯(代号PT-28)在硝酸介质中萃取三价镧系元素的行为,讨论了萃取剂浓度的变化、硝酸钠的浓度及温度变化对萃取率的影响,并对PT-28. 萃取HNO_3的机理进行了探讨。

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The extracts obtained from 28 species of marine algae were evaluated for their antioxidant activity (AA) versus the positive controls butylated hydroxytoluene (BHT), gallic acid (GA), and ascorbic acid (AscA). Most of the tested samples displayed antioxidant activity to various degrees. Among them, the extract of Symphyocladia latiuscula exhibited the strongest AA, which was comparable to BHT, GA, and AscA in radical scavenging activity, as shown in the DPPH (alpha,alpha-diphenyl-beta-picrylhydrazyl) assay, and higher than those of the positive controls in beta-carotene-linoleate assay system. In addition, the ethyl acetate-soluble fraction isolated from the crude extract of S. latiuscula exhibited the highest antioxidant activity in both assay systems. This fraction was further fractionated into seven subfractions (F1-F7) by vacuum liquid chromatography (VLC). F1 and F4 were found to be the most effective subfractions in scavenging DPPH radical assay and in the beta-carotene-linoleate assay, respectively. The total phenolic content (TPC) and reducing power (RP) for all of the extracts, fractions, and subfractions (F1-F7) were also determined. The TPC of the 28 extracts ranged from 0.10 to 8.00 gallic acid equivalents (mg/g seaweed dry weight) while the RP ranged from 0.07 to 11.60 ascorbic acid equivalents (mg center dot g(-1) seaweed dry weight). Highly positive relationships between AA and TPC as well as between AA and RP were found for the extracts and fractions, while for the subfractions F1-F7 only weak or no such relations were found. The results obtained from this study indicate that further analysis is needed of those marine algal species that contain the most antioxidant activity in order to identify the active principles.

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海湾扇贝Argopecten irradian Lamarck于1982年从美国引种到中国,由于具有较快的生长速度和很高的经济效益,海湾扇贝成为中国最主要的养殖贝类之一。近年来海湾扇贝养殖遇到了死亡率高等问题,深入开展海湾扇贝功能基因的研究,尤其是免疫相关基因及其机制研究并在此基础上寻找扇贝疾病防治的有效方法对海湾扇贝的健康养殖十分重要。 对于贝类免疫系统来说,其血细胞在先天性免疫防御中起着重要的作用。当受到外界病原侵染时,贝类血细胞的一个重要免疫反应就是吞噬作用。在吞噬病原过程中,受到病原侵染的贝类还会产生其他多种免疫反应,这些免疫反应将消耗大量的能量(ATP),产能的呼吸链会加速运转,由此也会引发与呼吸链相耦联的活性氧(ROS)的大量产生。这些活性氧具有极强的反应特性,能破坏病原微生物的结构和功能分子,实现对入侵病原的杀灭。利用活性氧对被吞噬的病原进行杀灭,这是吞噬作用消除病原抵御侵染的重要机制。但由于活性氧分子反应的非特异性,它们也会破坏宿主机体细胞内的功能蛋白分子、不饱和脂肪酸分子和核酸等,对细胞造成严重的伤害,进而导致机体生理机能的损伤和免疫系统的破坏。所以,及时消除病原感染机体内过量产生的ROS,维持相关细胞的正常代谢,对提高机体抵抗力和免疫力具有重要的作用。O2-是生物体内产生的第一种活性氧分子,其他的活性氧分子也是由它衍生而来,消除过量O2-是消除过量活性氧危害的第一步也是关键一步。生物体内,超氧化物歧化酶(SOD)是催化O2-发生歧化反应,消除O2-的关键酶。 首先,本文通过RACE方法获得了海湾扇贝SOD家族全部三种基因的cDNA全长并对其进行了序列的生物信息学分析,海湾扇贝AiCuZnSOD全长cDNA为1047个碱基,其中开放阅读框为459个碱基,编码152个氨基酸,与栉孔扇贝Chlamys farreri的CuZnSOD相似度为77.5%,与长牡蛎Crassostrea gigas的相似度为75%,与人的相似度为74.7%。AiMnSOD全长cDNA为1207个碱基,其中开放阅读框为678个碱基,编码226个氨基酸,序列比对结果发现AiMnSOD的氨基酸序列与虾夷扇贝Mizuhopecten yessoensis和皱纹盘鲍Haliotis discus hannai的相似度分别为85%和78.4%,与哺乳动物相似度也在68%~72%之间。AiECSOD全长cDNA为893个碱基,其中开放阅读框为657个碱基,编码218个氨基酸。AiECSOD与其它物种ECSOD相似度比较低。与线虫Brugia pahangi的相似度为27.9%,与疟蚊Anopheles gambiae的相似度为31.4%,与斑马鱼Danio rerio的相似度为27.8%,与人的相似度也只有28.6%,与同是贝类的长牡蛎ECSOD也只有28.1%的相似性。主要原因是AiECSOD的信号肽和肝磷脂结合区域在各物种中无同源性。 其次,采用qRT-PCR(quantitative real time PCR)方法分析三种SOD基因在不同组织中的表达情况,结果表明三种SOD基因的组织表达有所差异。AiCuZnSOD基因在鳃中表达水平最高,其次是血细胞和性腺,在外套膜、闭壳肌和肝胰脏表达水平较低。AiMnSOD基因在鳃中表达水平最高,其次是外套膜,在血细胞、性腺,而在肝胰脏和闭壳肌表达较弱。AiECSOD基因在血细胞中表达水平最高,其次是肝胰脏,在鳃、闭壳肌表达水平较低,而性腺和外套膜没有检测到。同时,采用qRT-PCR对鳗弧菌Vibrio angullarum感染后海湾扇贝血细胞中三种SOD基因mRNA表达变化进行了检测。AiCuZnSOD表达量在各个时间段没有显著差异(P > 0.05)。AiMnSOD的表达量在1.5 h时略有下降,在3 h时达到最高表达量,是空白组(0h)的3倍(P < 0.01),从6 h到24 h表达量逐渐下降,24 h时表达量是空白组的1.6倍,24 h到48 h又稍有升高。AiECSOD的表达量在1.5 h时有所下降,是空白组的0.3倍(P < 0.05),随后逐渐升高,在12 h时达到最高表达量,是空白组(0h)的4.5倍(P < 0.01),从24 h到48 h表达量逐渐下降并恢复到空白组的水平。在对照组,各个时间点没有显著差异(P > 0.05)。在鳗弧菌感染后,海湾扇贝三种SOD的表达并不一致,且差异比较显著。AiCuZnSOD被认为是构成性表达基因,其受外界刺激的影响最小,AiMnSOD和AiECSOD受刺激后表达上调比较明显。 第三,采用Genome-walking的方法得到了海湾扇贝三种SOD基因的基因组全长和近端启动子序列并对其进行了相关分析。AiCuZnSOD的基因组序列全长为4279bp,包含有4个外显子和3个内含子。AiMnSOD的基因组序列全长为10692bp,包含有4个外显子和3个内含子。AiECSOD的基因组序列全长为5276bp,包含有5个外显子和4个内含子。三种基因外显子和内含子的结合处序列遵循-AT/GT-原则。我们把海湾扇贝SOD家族的三个基因的近端启动子进行了比较分析。发现三种SOD在靠近起始密码子的位置都有Oct-1结合位点。三种SOD共有的转录位点有:Oct-1、C/EBPalp、Oct2.1、Sp-1和GATA-1。AiCuZnSOD和AiMnSOD共有的转录位点有:ICSBP、Ftz、TATA-box、C/EBPbeta和Antp。AiCuZnSOD和AiECSOD共有的转录位点有:AP-1和NFκB。AiMnSOD和AiECSOD共有的转录位点有:GR和ER。AiCuZnSOD独有的位点有:SRF、YY-1和NF-1。AiMnSOD独有的位点有:HNF-1、Hb、MEB、NF-muE1、Pit-1a和Eve。AiECSOD独有的位点有:CREB、RATA-alph、Kruppel-like和AP-3。 此外,通过构建原核表达载体,本研究对AiCuZnSOD和AiECSOD基因进行了体外重组表达,并对纯化的重组蛋白进行了酶活分析。酶活分析表明,重组AiCuZnSOD蛋白有较高的酶活和稳定性。 最后,我们对海湾扇贝三种SOD基因的部分区域,包括启动子、编码区,部分内含子区域进行了SNP检测,并对SOD基因部分SNP位点多态性和鳗弧菌敏感性进行了相关分析。三种SOD基因中,我们共发现了59个SNP位点,其中AiECSOD的SNP位点最多,特别是在启动子区,AiCuZnSOD和AiMnSOD多态性较低。其中AiCuZnSOD启动子区的-1739 T-C 位点的基因型和等位基因,AiECSOD启动子区的-498 A-T和-267 G-A等位基因频率,AiECSOD的第一个外显子38 Thr-Lys的多态性在敏感和抗菌群体中存在显著差异(P < 0.05)。

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2010

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2010

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2010

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BACKGROUND: Despite numerous studies on endotracheal tube cuff pressure (CP) management, the literature has yet to establish a technique capable of adequately tilling the cuff with an appropriate volume of air while generating low CP in a less subjective way. the purpose of this prospective study was to evaluate and compare the CP levels and air volume required to fill the endotracheal tubes cuff using 2 different techniques (volume-time curve versus minimal occlusive volume) in the immediate postoperative period after coronary artery bypass grafting. METHODS: A total of 267 subjects were analyzed. After the surgery, the lungs were ventilated using pressure controlled continuous mandatory ventilation, and the same ventilatory parameters were adjusted. Upon arrival in the ICU, the cuff was completely deflated and re-inflated, and at this point the volume of air to fill the cuff was adjusted using one of 2 randomly selected techniques: volume-time curve and minimal occlusive volume. We measured the volume of air injected into the cuff, the CP, and the expired tidal volume of the mechanical ventilation after the application of each technique. RESULTS: the volume-time curve technique demonstrated a significantly lower CP and a lower volume of air injected into the cuff, compared to the minimal occlusive volume technique (P < .001). No significant difference was observed in the expired tidal volume between the 2 techniques (P = .052). However, when the subjects were submitted to the minimal occlusive volume technique, 17% (n = 47) experienced air leakage as observed by the volume-time graph. CONCLUSIONS: the volume-time curve technique was associated with a lower CP and a lower volume of air injected into the cuff, when compared to the minimal occlusive volume technique in the immediate postoperative period after coronary artery bypass grafting. Therefore, the volume-time curve may be a more reliable alternative for endotracheal tube cuff management.