Abr and Bcr are multifunctional regulators of the Rho GTP-binding protein family.

Philadelphia chromosome-positive leukemias result from the fusion of the BCR and ABL genes, which generates a functional chimeric molecule. The Abr protein is very similar to Bcr but lacks a structural domain which may influence its biological regulatory capabilities. Both Abr and Bcr have a GTPase-activating protein (GAP) domain similar to those found in other proteins that stimulate GTP hydrolysis by members of the Rho family of GTP-binding proteins, as well as a region of homology with the guanine nucleotide dissociation-stimulating domain of the DBL oncogene product. We purified as recombinant fusion proteins the GAP- and Dbl-homology domains of both Abr and Bcr. The Dbl-homology domains of Bcr and Abr were active in stimulating GTP binding to CDC42Hs, RhoA, Rac1, and Rac2 (rank order, CDC42Hs > RhoA > Rac1 = Rac2) but were inactive toward Rap1A and Ha-Ras. Both Bcr and Abr acted as GAPs for Rac1, Rac2, and CDC42Hs but were inactive toward RhoA, Rap1A, and Ha-Ras. Each individual domain bound in a noncompetitive manner to GTP-binding protein substrates. These data suggest the multifunctional Bcr and Abr proteins might interact simultaneously and/or sequentially with members of the Rho family to regulate and coordinate cellular signaling.

NusG is required to overcome a kinetic limitation to Rho function at an intragenic terminator.

Rho-dependent transcription termination at certain terminators in Escherichia coli also depends on the presence of NusG [Sullivan, S. L. & Gottesman, M. E. (1992) Cell 68, 989-994]. We have found that termination at the first intragenic terminator in lacZ (tiZ1) is strongly dependent on NusG when transcription is done in vitro with the concentrations of NTPs found in vivo. With a lower level of NTPs, and consequently a slower rate of RNA-chain growth, Rho causes some termination by itself that is enhanced with NusG. These results suggest that NusG serves to overcome a kinetic limitation of Rho to function at certain terminators. At a second intragenic terminator within the lacZ reading frame (tiZ2) the efficiency of Rho-mediated termination was unaffected by either NusG or by RNA polymerase elongation kinetics. Thus, using purified components and intracellular levels of NTPs, we have confirmed the in vivo finding that certain Rho-dependent terminators also depend on NusG, whereas others do not.

Statuto organico dell'Ospitale Casati sotto il titolo di S. Giuseppe in Passirana : frazione del comune di Lainate, mandamento di Rho, circondario di Gallarate, provincia di Milano.

Former shelf-mark: Cavagna 1036 copy 2.

Laboratory study of wave energy losses by bottom friction and percolation.

Mode of access: Internet.

Ioannis Rho ... Societatis Iesu, Ad Io. Bapt. Castaldum Clericum Regularem Interrogationes apologeticae : in quibus S. Ignatij ... cum B. Caietano Thienaeo colloquentis atque ab eo Theationorum Ordine ... reiicitur fabula.

Progressus Ordinis Clericorum Regularium ab anno XXIV ..., p. 216-218 ; Progressiones Societatis Iesu ab anno XXXIX ..., p. 219-241.

Description physique et politique du département du Rhône;

Mode of access: Internet.

Ioannis Rho e Societate Iesu In Passionem Domini cogitationes variae : in libros quatuor distinctae.

Mode of access: Internet.

Examen des nouveaux écrits de la Provence sur la proprieté du Rhône.

Mode of access: Internet.

Studiehandbok för de studerande vid Veterinärhögskolan, utgiven 1920.

Mode of access: Internet.

Beiträge zur Siedelungskunde der Südlichen Rhön und des Fränkischen Saaletals /

Includes bibliographical references.

Urkundliche Geschichte des reichsritterlichen Geschlechtes Eberstein vom Eberstein auf der Rhön /

Mode of access: Internet.

Loran-C RHO-RHO secondary phase correction table : north Atlantic /

Chiefly tables.

Uber die einwirkung von hydrazinen auf rhodanessigsaure und rho...

Thesis (doctoral)--

Regolamento dell'Ospitale Casati sotto il titolo di S. Giuseppe in Passirana : frazione del comune di Lainate, mandamento di Rho, circondario di Gallarate, provincia di Milano : approvato dalla Deputazione provinciale col decreto 28 gennaio 1876. 850 - B.

University of Illinois bookplate: "From the library of Conte Antonio Cavagna Sangiuliani di Gualdana Lazelada di Bereguardo, purchased 1921".