Molecular profiling of sepsis in mice using Fourier Transform Infrared Microspectroscopy

Sepsis is a life threatening condition resulting from a high burden of infection. It is a major health care problem and associated with inflammation, organ dysfunction and significant mortality. However, proper understanding and delineating the changes that occur during this complex condition remains a challenge. A comparative study involving intra-peritoneal injection of BALB/c mice with Salmonella Typhimurium (infection), lipopolysaccharide (endotoxic shock) or thioglycollate (sterile peritonitis) was performed. The changes in organs and sera were profiled using immunological assays and Fourier Transform Infrared (FTIR) micro-spectroscopy. There is a rapid rise in inflammatory cytokines accompanied with lowering of temperature, respiratory rate and glucose amounts in mice injected with S. Typhimurium or lipopolysaccharide. FTIR identifies distinct changes in liver and sera: decrease in glycogen and protein/lipid ratio and increase in DNA and cholesteryl esters. These changes were distinct from the pattern observed in mice treated with thioglycollate and the differences in the data obtained between the three models are discussed. The combination of FTIR spectroscopy and other biomarkers will be valuable in monitoring molecular changes during sepsis. GRAPHICS] Intra-peritoneal infection with high dose of Salmonella Typhimurium leads to rapid increase in inflammatory cytokines, e.g. Tnf alpha (A). FTIR analysis of liver (B) and sera (C) identifies several metabolic changes: glycogen, protein/lipid, cholesteryl esters and DNA.

Fuzzy Approach to Rank Global Climate Models

Eleven coupled model intercomparison project 3 based global climate models are evaluated for the case study of Upper Malaprabha catchment, India for precipitation rate. Correlation coefficient, normalised root mean square deviation, and skill score are considered as performance indicators for evaluation in fuzzy environment and assumed to have equal impact on the global climate models. Fuzzy technique for order preference by similarity to an ideal solution is used to rank global climate models. Top three positions are occupied by MIROC3, GFDL2.1 and GISS with relative closeness of 0.7867, 0.7070, and 0.7068. IPSL-CM4, NCAR-PCMI occupied the tenth and eleventh positions with relative closeness of 0.4959 and 0.4562.

Fourier Transform Method for Measuring the Micro Amplitu of Water Surface Waves

采用一种非接触的光学方法傅立叶变换莫尔法(Fourier transform method),结合数字图像处理技术,对微幅振荡的水表面波的振幅进行测量.它是对全场中每一个像素点进行测量,比接触测量法具有更高的灵敏度.它为微幅水表面波振幅的测量提供了一种手段.通过将计算机生成的周期性光栅图像经投影机直接投影到被测物体的参考平面,经CCD摄像头、图像板捕捉存储形成数字化的光栅图像,利用傅立叶变换莫尔法处理光栅图像,从而获得包含有水表面波的振幅的相位信息,再经适当的几何变换获得振幅信息.我们在垂直振荡装置上进行了不同激励频率和不同振幅的表面波的振幅测量.

Using wavelet transform to study the Lipschitz local singular exponent in wall turbulence

In this paper, wavelet,transform is introduced to study the Lipschitz local singular exponent for characterising the local singularity behavior of fluctuating velocity in wall turbulence. I, is found that the local singular exponent is negative when the ejections and sweeps of coherent structures occur in a turbulent boundary layer.