964 resultados para métodos de identificação
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Pós-graduação em Química - IQ
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
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Pós-graduação em Engenharia Mecânica - FEIS
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Pós-graduação em Medicina Veterinária - FCAV
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Pós-graduação em Fisiopatologia em Clínica Médica - FMB
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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The scope of this work is the study of natural cavities in gullies through geophysics. The studied area is located in the city of São Pedro (SP) more precisely in the gully of Tucunzinho. The historic of the area shows that, since the 60s there were problems with the high rate of erosion in the gully. In addition to increased erosion, there is the appearance of cavities inside responsible for rebates, aggravating the situation. Geophysical methods have been successfully applied in environmental studies since they are noninvasive, are fast and relatively inexpensive. In order to better understand the context of formation of the pipes in relation to local geology, three arrangements were compared for the method of Electrical Resistivity, Schlumberger, Wenner and Dipole-dipole. Then, it was possible to determine which one is the best for this type of study. According to the data obtained in the field, the Schlumberger array presents more consistent results in relation to the erosive context analyzed
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The identification of Salmonella spp. in food samples by microbiological diagnosis is time consuming, with approximately five different stages, requiring about 120 hours until the final result. The utilization of the polymerase chain reaction technique (PCR) can reduce this time, but substances present in samples may affect the reaction. The present work aimed to compare DNA extraction by thermic treatment and by the use of cetyltrimethil ammonium bromide (CTAB), in products originated from poultry houses corresponding to raw material (meat meal) and experimentally contaminated drag swabs. Materials obtained from the extractions were submitted to PCR, utilizing a pair of initiator oligonucleotides for amplification of Sdf 1 gene fragments. Comparing the methods of extraction, it was observed that when CTAB was employed, SE was detected in 70% of meat meal and in 80% of drag swabs, while the thermic treatment method yielded positive results in 20% of meat meal and in 40% of drag swabs. SE was detected under both methods utilized for DNA extraction, but the use of CTAB detected a greater number of positive samples, compared with thermal treatment.
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
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This study used a multi-analytical approach based on traditional microbiological methods for cultivation and isolation of heterotrophic bacteria in the laboratory associated with the molecular identification of the isolates and physicochemical analysis of environmental samples. The model chosen for data integration was supported by knowledge from computational neuroscience, and composed by three modules: (i) microbiological parameters, contemplating taxonomic data obtained from the partial sequencing of the 16S rRNA gene from 80 colonies of heterotrophic bacteria isolated by plating method in PCA media. For bacterial colonies isolation were used water samples from Atibaia and Jaguarí rivers collected at the site of water captation for use in effluent treatment, upstream from the entrance of treated effluent from the Paulínia refinery (REPLAN/Petrobras) located in the Paulínia-SP municipality, from the output of the biological treatment plant with stabilization pond and from the raw refinery wastewater; (ii) chemical parameters, ending measures of dissolved oxygen (DO), chemical oxygen demand (COD), biochemical oxygen demand (BOD), chloride, acidity CaCO3, alkalinity, ammonia, nitrite, nitrate, dissolved ions, sulfides, oils and greases; and (iii) physical parameters, comprising the pH determination, conductivity, temperature, transparency, settleable solids, suspended and soluble solids, volatile material, remaining fixing material (RFM), apparent color and turbidity. The results revealed interesting theoretical relationships involving two families of bacteria (Carnobacteriaceae and Aeromonadaceae). Carnobacteriaceae revealed positive theoretical relationships with COD, BOD, nitrate, chloride, temperature, conductivity and apparent color and negative theoretical relationships with the OD. Positive theoretical relationships were shown between Aeromonadaceae and OD and nitrate, while this bacterial family showed negative theoretical...
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A seleção de pessoal visa a introduzir na organização os candidatos mais apropriados as suas necessidades. Existe uma carência de literatura behaviorista radical acerca desse tema, sendo necessário o desenvolvimento de trabalhos que orientem atuações coerentes com essa teoria. O objetivo deste estudo é discutir os métodos utilizados na seleção à luz dos pressupostos conceituais do behaviorismo radical. Os métodos de seleção existentes são analisados e discutidos em termos de aumento do controle das variáveis presentes no contexto da seleção a fim de proceder à identificação dos repertórios comportamentais dos candidatos. Propõe-se uma análise de cargo baseada nos conceitos behavioristas, discute-se a possibilidade de condução de entrevistas de seleção focadas no comportamento bem como o uso de técnicas de simulação que permitam a identificação de repertórios comportamentais. Conclui-se que é necessário desenvolver discussões teóricas e instrumentos operacionais facilitadores de uma prática psicológica vinculada aos pressupostos behavioristas radicais.