Methicillin-resistant Staphylococcus aureus: phylogenetic relatedness between European epidemic clones and Swiss sporadic strains.

We have compared the phylogenetic diversity of methicillin-resistant Staphylococcus aureus (MRSA) strains from Switzerland and their phylogenetic relationships with European epidemic clones, using multiprimer random amplification polymorphic DNA (RAPD). Strains included 24 European epidemic clones (59 strains), 66 sporadic strains isolated in Switzerland in 1996-1997, and 15 reference strains of five other Staphylococcus species. Similarity and clustering analysis with the Jaccard's coefficient showed that the maximum genetic distance between MRSA strains was 0.43, whereas the minimum genetic distance between the six Staphylococcus species was 0.97, indicating that the method permits phylogenetic hierarchization. The 24 MRSA clones reported to be epidemic in European countries during the 1990s were distributed into seven different genetic clusters with a maximum distance of 0.29 among them. This clustering pattern was confirmed by the analysis of a subset of MRSA strains by multilocus enzyme electrophoresis at 12 loci. Most of the sporadic Swiss strains were distributed into these seven different genetic clusters, together with the epidemic MRSA clones. This suggests that there is no phylogenetic cluster specific to epidemic clones of MRSA.

Competitiveness Comparison of the Electricity Production Alternatives

The competitiveness comparison is carried out for merely electricity producing alternatives. In Finland, further construction of CHP (combined heat and power) power plants will continue and cover part of the future power supply deficit, but also new condensing power plant capacity will be needed. The following types of power plants are studied: - nuclear power plant, - coal-fired condensing power plant - combined cycle gas turbine plant, - peat-fired condensing power plant. - wood-fired condensing power plant - wind power plant The calculations have been made using the annuity method with a real interest rate of 5 % perannum and with a fixed price level as of March 2003. With the annual full load utilization time of 8000 hours the nuclear electricity would cost 23,7 ¤/MWh, the gas based electricity 32,3 ¤/MWh and coal based electricity 28,1 ¤/MWh. If the influence of emission trading is taken into account,the advantage of the nuclear power will still be improved. Inorder to study the impact of changes in the input data, a sensitivity analysis has been carried out. It reveals that the advantage of the nuclear power is quite clear. E.g. the nuclear electricity is rather insensitive tothe changes of the uranium price, whereas for natural gas alternative the rising trend of gas price causes the greatest risk.

Alternatives per a l’engreix ecològic de bovins: la sal com a element regulador del consum de pinso en vedells de la raça Parda de Montaña

El present treball proposa l’addició d’un 10% de sal al pinso d’engreix d’11 vedells de la raça Parda de Montaña, com a sistema per limitar-ne el consum sense haver de fer ús de mà d’obra complementària ni de sistemes mecanitzats que en controlin la quantitat de consum. Aquest % de sal, pretén aconseguir una reducció en la palatabilitat del pinso de consum dels animals, aconseguint així uns menors valors d’ingestió de concentrat per un major consum de farratge. Aquesta relació quantitativa té com a objectiu, aconseguir la introducció d’aquests animals en el marc de la producció ecològica, que estableix una ràtio de consum de farratge vs concentrat de 60 : 40. El grup d’11 vedells, prèviament esmentat, és comparat amb una altre grup, també d’11 vedells i de la mateixa raça i edat, que són alimentats amb el mateix fenc d’alfals, però amb pinso convencional i no amb el complementat amb sal

Nature of the Epidemic Threshold for the Susceptible-Infected-Susceptible Dynamics in Networks

We develop an analytical approach to the susceptible-infected-susceptible epidemic model that allows us to unravel the true origin of the absence of an epidemic threshold in heterogeneous networks. We find that a delicate balance between the number of high degree nodes in the network and the topological distance between them dictates the existence or absence of such a threshold. In particular, small-world random networks with a degree distribution decaying slower than an exponential have a vanishing epidemic threshold in the thermodynamic limit.

Estudi d'alternatives de gestió de la fracció orgànica generada al municipi de l'Esquirol

L'entrada en vigor de la Directiva 1999/31/CE limita l'entrada de residus biodegradables a l'abocador i promou la gestió d'aquests residus per altres vies. El compostatge és una alternativa a la deposició en abocador que respecta el cicle natural de la matèria. A Catalunya, aquesta tècnica s'ha vist potenciada pels plans de gestió de residus PRECAT201 i PINFRECAT202. L'objecte d'aquest estudi és avaluar l'eficiència de la recollida selectiva al municipi de l'Esquirol; i comparar i avaluar diferents alternatives de gestió de la FORM3 dins i fora del municipi. La metodologia de l'estudi ha consistit en analitzar les dades de la recollida selectiva de l'ARC4, tant del municipi de l'Esquirol com de la resta de municipis del Collsacabra, i calcular els costos econòmics de la gestió de la FORM en el supòsit de tractament mitjançant l'autocompostatge, una planta de compostatge a petita escala o el tractament en una planta de compostatge convencional. Els resultats de l'estudi indiquen que la recollida selectiva del municipi de l'Esquirol obté uns valors per sobre dels objectius del PROGREMIC5, amb l'excepció de la FORM. Per tal de millorar la qualitat i la quantitat de la FORM recollida, l'estudi proposa en una primera fase: implantar el sistema de recollida selectiva porta a porta d'aquesta fracció i de la fracció resta; i en una segona fase: tractar la FORM en una petita planta de compostatge amb el sistema de sitges amb ventilació forçada.

Soins à domicile, soins en EMS: de quelles alternatives dispose la personne âgée dépendante de soins?

La dépendance de soins due à l'âge est un phénomène dont l'importance n'a cessé de croître ces dernières années. Différentes alternatives s'offrent aux personnes dépendantes. Les principales sont le recours à des prestations d'aide et de soins à domicile ou le long séjour dans un établissement médico-social (EMS). Au vu des coûts occasionnés par leur dépendance, il est relativement fréquent que les personnes âgées bénéficient d'aides financières ; celles-ci prennent la forme de prestations complémentaires à l'AVS/AI et peuvent être complétées, au besoin, par diverses aides cantonales.

Statistical Analysis of an SEIR Epidemic Model

This thesis was focussed on statistical analysis methods and proposes the use of Bayesian inference to extract information contained in experimental data by estimating Ebola model parameters. The model is a system of differential equations expressing the behavior and dynamics of Ebola. Two sets of data (onset and death data) were both used to estimate parameters, which has not been done by previous researchers in (Chowell, 2004). To be able to use both data, a new version of the model has been built. Model parameters have been estimated and then used to calculate the basic reproduction number and to study the disease-free equilibrium. Estimates of the parameters were useful to determine how well the model fits the data and how good estimates were, in terms of the information they provided about the possible relationship between variables. The solution showed that Ebola model fits the observed onset data at 98.95% and the observed death data at 93.6%. Since Bayesian inference can not be performed analytically, the Markov chain Monte Carlo approach has been used to generate samples from the posterior distribution over parameters. Samples have been used to check the accuracy of the model and other characteristics of the target posteriors.

The use of non-animal alternatives in the safety evaluations of cosmetics ingredients by the Scientific Committee on Consumer Safety (SCCS)

In Europe, the safety evaluation of cosmetics is based on the safety evaluation of each individual ingredient. Article 3 of the Cosmetics Regulation specifies that a cosmetic product made available on the market is to be safe for human health when used normally or under reasonably foreseeable conditions. For substances that cause some concern with respect to human health (e.g. colorants, preservatives, UV-filters), safety is evaluated at the Commission level by a scientific committee, presently called the Scientific Committee on Consumer Safety (SCCS). According to the Cosmetics Regulations, in the EU, the marketing of cosmetics products and their ingredients that have been tested on animals for most of their human health effects, including acute toxicity, is prohibited. Nevertheless, any study dating from before this prohibition took effect is accepted for the safety assessment of cosmetics ingredients. The in vitro methods reported in the dossiers summited to the SCCS are here evaluated from the published reports issued by the scientific committee of the Directorate General of Health and Consumers (DG SANCO); responsible for the safety of cosmetics ingredients. The number of studies submitted to the SCCS that do not involve animals is still low and in general the safety of cosmetics ingredients is based on in vivo studies performed before the prohibition.