Desenvolvimento inicial de clones IAC de seringueira em São José do Rio Preto, SP

In this study the development of four clones (IAC 35; 40; 300 e 301) of rubber tree [Hevea brasiliensis (Willd. ex A. Juss.) Müell. Arg.] selected by Instituto Agronômico de Campinas were evaluated during the first 24 months of cultivation. The experiment was laid out in randomized block design with three replications and four plants for parcel following the 1.5mx1.0m spacing at São José do Rio Preto, SP. The clone RRIM 600 was used as check. The variables analyzed were the trunk girth, the number of matured whorl, the photosynthesis rate and the concentrations of chlorophyll a, b, total and carotenoids. At the end of the experiment the values of trunk girth at 0.5m above the budgrafting union varied from 5.85 cm (IAC 301) to 10.53 cm (IAC 300) and the mean number of matured whorl varied from 2.58 (IAC 301) to 3.91 (RRIM 600). During the dry season, when the plants were 22 month old, the mean value of photosynthesis rate of IAC 40 and IAC 301 (12mol m-2 s-1) were lower compared to the other clones (15mol m-2 s-1). The chlorophyll a, b and total carotenoids concentrations were equal or superior to the check, never lower. Considering the variables analyzed, excluding the IAC 301, the other clones show performance comparable to RRIM 600.

Expressão, purificação e caracterização de proteínas dos fitovírus SBMV e RSPaV

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Desenvolvimento inicial de maracujazeiros (Passiflora edulis f. flavicarpa, P. edulis f. edulis e P. alata) enxertados sobre Passiflora cincinnata

The present work aimed to evaluate the initial growth and leaf mineral levels in passion fruit trees (Passiflora edulis f. flavicarpa Deg., P. edulis f. edulis Sims. and P. alata Dryander) grafted onto Passiflora cincinnata. To obtain seedlings, seeds were sown in plastic bags (500 mL) and hypocotyl grafting was performed when seedlings reached the stage of two fully expanded leaves. Fifteen days after grafting, plants were transplanted to 10L pots filled with previously limed and fertilized soil. Each pot contained two plants and corresponded to one plot. For each commercial species studied as rootstock, experimental design was completely randomized, in 3x5 (plant type x time of harvest) factorial arrangement, with four replicates of two plants per plot and five destructive harvests. Plant types were ungrafted P. cincinnata, ungrafted commercial passion fruit tree and commercial passion fruit tree grafted onto P. cincinnata. The first harvest was performed at 15 days after transplanting and the remaining ones at 14-day intervals (60, 74, 88, 102 and 116 DAS). At each harvest, the number of leaves per plant was counted, and leaf area, stem length, and stem, root, leaf and total dry matter were estimated. At the last harvest, the mineral composition (macro and micronutrients) of plants was analyzed. In general, it was observed that grafting onto P. cincinnata did not interfere negatively with the initial development and mineral levels of commercial passion fruit trees, and this interference varied according to the used canopy.

Epitelização de enxertos cutâneos em feridas recentes de coelhos tratados com membrana amniótica canina e/ou laserterapia

Pós-graduação em Cirurgia Veterinária - FCAV

Manutenção de dois sistemas radiculares em aspectos bioquímicos e fisiológicos durante o desenvolvimento de plantas e desempenho agronômico de Passiflora edulis Sims enxertadas

Pós-graduação em Agronomia (Horticultura) - FCA

Enxerto ósseo em odontologia: revisão de literatura

Bone is a specialized connective tissue, vascular and dynamic changes over the life of the organism. When injured, has a unique ability to regenerate and repair without the presence of scars, but in some situations due to the size of the defect, the bone tissue does not regenerate so completely, it is necessary to carry out bone grafting procedures. Considering there are various types of grafts and various donor sites. Thus, the aim of this study was to review the literature to some type of graft most commonly used in dentistry. Given the importance of bone reconstruction in oral and maxillofacial surgery, it is necessary to know the viability and influence of biomaterials, or not associated with autogenous grafts in bone repair. Even this, with many qualities, but further studies should be done to achieve each day, a synthetic material compatible with bone tissue lost in adequate amounts without requiring extra-oral surgeries that are considered to be devoted to higher morbidity.

Reabilitação de maxila atrófica com tecido ósseo homógeno e prótese fixa implantossuportada

The techniques of bone reconstruction for atrophic maxillae have been improved in order to promote bone tissue growth in both height and thickness. The grafts performed with use of autogenous bone is considered the gold standard by most researchers, for demonstrating osteogenic capacity and not to promote antigenic response. However, this type of grafting is not possible to get bone tissue in large quantity for extensive renovations. In recent years, alternatives have been researched to overcome the limitations of autogenous bone. Several alternatives have been investigated to supply the disadvantages of autogenous bone grafts. In such studies, allogeneic bone grafts which are obtained from individuals with different genetic load, but from the same species have been extensively used. They can be indicated in cases of arthrosplasty, surgical knee reconstruction, and large bone defects as well as in oral and maxillofacial reconstruction. Besides showing great applicability and biocompatibility, this type of bone is available in unlimited quantities. To rehabilitate atrophic maxillae an option that has been performed with high success rate is the reconstruction with bone graft followed by osseointegrated dental implants to rehabilitate the patient aesthetics and functionally. This paper aims to show the feasibility of allogenic bone as material for reconstruction of atrophic maxilla, and subsequent rehabilitation with metal ceramic fixed prosthesis implant and dental restoration with accompanying three years through literature review and clinical case report.

Avaliação do índice de sucesso dos enxertos autógenos e dos implantes instalados em região de seio maxilar

Maxillary sinus lifting surgery is considered an excellent option for patient rehabilitation in cases of maxillary sinus pneumatization. This longitudinal study was performed in 41 patients to evaluate 51 maxillary sinus lifting procedures concomitant to autogenous bone grafted from mandibular ramus. Of the 107 implants installed, 28 were immediate fixations. Success index achieved regarding to bone graft was of 98.04%. The implants installed immediately or after bone grafting resulted in success rates of 92.86% and 94.59% respectively, with no statistically significant differences between them. Surgeries for bone grafting and immediate installation are considered a reliable treatment alternative provided that both anatomical structures and tissue physiology are kept in good conditions, with appropriate indication and techniques for good implant stability.

Resposta enzimática, fisiológica e produtiva do tomateiro e desempenho de porta enxertos resistentes à murcha bacteriana

Pós-graduação em Agronomia (Horticultura) - FCA

Germinação de sementes de maracujazeiros: água, luz, temperatura e reguladores vegetais

The production of seedlings of passion fruit is done mainly with the use of seeds by most producers. It can also be propagated by cuttings, layering and in vitro tissue culture. However, even when grafting is used, it is necessary to use seeds to produce the rootstock. Nevertheless, most Passifloracea species have germination problems, making it difficult to obtain seedlings. Although several studies have been developed in an attempt to increase seed germination of passion fruit there are still difficulties in understanding the germination process and also disagreement about seed dormancy. Thus, the present study aimed to review papers related to seed germination of passion fruit emphasizing aspects such as mechanical resistance to water ingress, the need for adequate light and temperature and the use of plant growth regulators in overcoming seed dormancy. It was noticed that, in general, the passion fruit seeds are photoblastic negative and exhibit physiological dormancy, being no need of seed scarification to stimulate its germination.

Sobrevivência de enxertos de Eucalyptus com metodologia adaptada

One of the important techniques to assist a tree improvement program is the grafting, which is used for the propagation of selected trees. The objective of this study was to evaluate the survival rate using a modified method of lateral grafting on Eucalyptus dunnii, Eucalyptus urophylla and a hybrid of Eucalyptus urophylla x Eucalyptus grandis. The work was conducted by the research teams of Eucatex and Palmasola companies, in September of 2011, and 589 grafts were made. The survival rate of the grafts was evaluated 180 days after grafting. Survival grafts rates were: 74% for E. dunnii, 55% for E. urophylla and 62% for the hybrid E. urophylla x E. grandis. These survival rates were higher than those currently obtained by the conventional method of eucalypt grafting. Thus, the method seems promising to assist in forest tree improvement programs, but further studies are still necessary to refine this grafting approach.

Polinização controlada em eucaliptos nas empresas florestais brasileiras

Controlled pollination is an important tool in a forest genetic improvement program, which permits to choose parents and make specific crosses maximizing gains and providing superior genotypes in less time than conventional pollination. To verify the needs of the forest companies regarding the conduction of controlled pollination orchards in the breeding program, a specific questionnaire was sent in September 2011 to companies associated to IPEF. The objective of the questionnaire was to collect basic information about the current situation of controlled pollination orchards and check the main difficulties and demands related. It was found that use of the controlled pollination orchard has begun to be used by companies in the Brazilian forest sector just a few years ago and is considered to be very important in breeding programs in order to shorten the time to obtain new genotypes; but there is still need of crucial information regarding the management of these orchards. The main demands are: Basic concepts of flowering induction; homogeneity of flowering; hormonal induction and alternative techniques for flowering; relationship between management and genotypes used; variations in the flowering of different genotypes; management of the orchard in the different phenological phases; process of grafting typesand interaction between graft and rootstock, irrigation systems; species identification and flowering map.

Macronutrientes em pepino enxertado em áreas com nematoides de galhas

Macronutrients in grafted cucumber in areas with gall nematodes. Grafting is an often recommended technique for cucumber culture in areas infested with gall nematodes. This study aimed to evaluate the content of macronutrients in grafted cucumber in two rootstocks on soil infested with Meloidogyne incognita race 2 or Meloidogyne javanica, since they may decrease the effectiveness of water and nutrient absorption. One evaluated 9 treatments (factorial 3x3: nongrafted hybrid cucumber Tsuyataro, grafted on squash hybrid Shelper or grafted on squash hybrid Excitte Ikki x without inoculation, inoculation with M. incognita or M. javanica), in a randomized blocks design, with 4 replicates and 6 plants per plot. The characteristics evaluated were: macronutrient content at 72 days after transplantation and macronutrient content at the end of the cycle (99 days after transplantation). The nutrient contents evaluated didn’t differ according to the nematode species (with or without inoculation), however, there’re differences with regard to the rootstocks, with higher values of phosphorus and potassium in grafted plants and higher values of magnesium, calcium, and sulfur in nongrafted plants at the end of cycle.

Clonagem do pessegueiro 'Aurora-1' e de portaenxertos de umezeiro

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Molecular characterization of a phytoplasma of group 16SrIX related to 'Ca. Phytoplasma phoenicium' in periwinkle in Brazil

Periwinkle (Catharanthus roseus), a tropical perennial plant, was found to be infected by a phytoplasma. Plants exhibiting virescence, phyllody and variegation symptoms were collected in the states of Minas Gerais and Sao Paulo, Brazil. The phytoplasma was transmitted by grafting from an infected periwinkle plant to healthy plants and by dodder to a citrus plant. Phytoplasma isolates from periwinkle plants from Brazil had the 16S rDNA gene sequenced and were classified in the 16SrIX group, subgroup A, belonging to the 'Candidatus P. phoenicium' species.