Role of Tyrosine Phosphorylation in Ligand-induced Regulation of Transcytosis of the Polymeric Ig Receptor

The polymeric Ig receptor (pIgR) transcytoses its ligand, dimeric IgA (dIgA), from the basolateral to the apical surface of epithelial cells. Although the pIgR is constitutively transcytosed in the absence of ligand, binding of dIgA stimulates transcytosis of the pIgR. We recently reported that dIgA binding to the pIgR induces translocation of protein kinase C, production of inositol triphosphate, and elevation of intracellular free calcium. We now report that dIgA binding causes rapid, transient tyrosine phosphorylation of several proteins, including phosphatidyl inositol-specific phospholipase C-γl. Protein tyrosine kinase inhibitors or deletion of the last 30 amino acids of pIgR cytoplasmic tail prevents IgA-stimulated protein tyrosine kinase activation, tyrosine phosphorylation of phospholipase C-γl, production of inositol triphosphate, and the stimulation of transcytosis by dIgA. Analysis of pIgR deletion mutants reveals that the same discrete portion of the cytoplasmic domain, residues 727–736 (but not the Tyr734), controls both the ability of pIgR to cause dIgA-induced tyrosine phosphorylation of the phospholipase C-γl and to undergo dIgA-stimulated transcytosis. In addition, dIgA transcytosis can be strongly stimulated by mimicking phospholipase C-γl activation. In combination with our previous results, we conclude that the protein tyrosine kinase(s) and phospholipase C-γl that are activated upon dIgA binding to the pIgR control dIgA-stimulated pIgR transcytosis.

Accident trend monitoring and exploratory analysis. Final report.

National Highway Traffic Safety Administration, National Center for Statistics and Analysis, Washington, D.C.

Primer año de lengua nacional;

Mode of access: Internet.

Guida delle Alpi occidentali /

University of Illinois Library bookplate "From the library of Conte Antonio Cavagna Sangiuliani di Gualdana Lazelada di Bereguardo, purchased 1921" on the inside front cover.

Gressoney : Ivrea, Pont St-Martin, Issime, Gressoney St-Jean, La Trinité, Monte Rosa.

"Estratto dalla Guida illustrata della valle d'Aosta."

Usual sources of medical care and their characteristics /

"September 1991"--P. [4] of cover.

Histoire des Celtes, et particulierement des Gaulois et des Germains, depuis Les Tems fabuleux, jusqu'à la Prisse de Rome par les Gaulois /

Mode of access: Internet.

The State records of North Carolina,

"Roster of the continental line from North Carolina, 1783": v. 16, p. [1002]-1197. Other rosters and lists of revolutionary soldiers in the collection are noted in the Index, v. 4 (Historical review, p. 95-96)

The colonial records of North Carolina.

Mode of access: Internet.

The state records of North Carolina.

The colonial and state records of North Carolina. vol. [XXVII]-XXX.

Familles historiques de Savoie; les seigneurs de Compey.

Includes bibliographies.

Southern history of the war. Official reports of battles,

Pages 1-31, (Report of General Beauregard of the battle of Manassas) is a reprint of p. 17-45 "Official reports of Generals Johnston and Beauregard of the battle of Manassas" Richmond, 1862. 144 p. The remainder of the volume is a reprint of "Official reports of battles. Published by order of Congress". Richmond, 1862. 571 p.

Literary estimate and bibliography of Richard Malcolm Johnston,

"From Publications of Southern history association, October, 1898."

Advance and retreat. Personal experiences in the United States and Confederate States armies.

Mode of access: Internet.

Alcune vedute di archi trionfali ed altri monumenti : inalzati da Romani parte de quali si veggono in Roma, e parte per l'Italia /

Described by Hind as renumbered from 1748 ed. In library's copy the plates have the earlier numbers and order but the additional plates of later eds. are included: Arco di Aosta and Tempio di Minerva Medica (the latter by Francesco Piranesi). Some plates numbered by pen.