Inbreeding depression in castor bean (Ricinus communis L.) progenies

O objetivo do trabalho foi investigar a depressão endogâmica (DE) na mamoneira, espécie de reprodução sexuada mista. de uma população derivada da cultivar Guarani, amostraram-se 60 plantas-mãe. de cada uma foram obtidos três tipos de progênies: de autofecundação (AU), de cruzamentos obrigatórios (CR) e de polinização livre (PL). A produtividade de grãos das progênies for avaliada por meio de experimentos em blocos incompletos em dois locais. Houve forte interação de progênies x locais o que levou a obter estimativas dentro de cada local. Verificou-se ampla variação na depressão endogâmica, com médias de 6,7% e 13,4%, comparando-se as progênies AU com as PL. Verificou-se que a população tem alto potencial para selecionar linhagens promissoras. Foi baixa a freqüência de plantas-mãe gerando progênies com alta capacidade geral de combinação e baixa depressão endogâmica, simultaneamente. Seleção recorrente aumentará a ocorrência de genitoras associando essas duas propriedades, necessárias para obtenção de variedades sintéticas superiores.

Air-assisted boom sprayer and spray deposition on bean plants

A possibilidade do desenvolvimento de técnicas de aplicação de produtos fitossanitários mais seguras, com menores volumes de calda, número de aplicações e deriva, aliados à necessidade de se obter melhores níveis de controle dos agentes nocivos às plantas cultivadas, justificam o uso da assistência de ar junto à barra de pulverização. Com o objetivo de avaliar a deposição da pulverização na cultura do feijoeiro (Phaseolus vulgaris), em presença e ausência da assistência de ar junto à barra de pulverização, com diferentes pontas de pulverização e volumes de calda, foi conduzido um experimento em delineamento inteiramente casualizado, utilizando-se como traçador o óxido cuproso. Alvos artificiais (papel filtro com 3 x 3 cm) foram afixados nas superfícies adaxial e abaxial de folíolos posicionados nos terços superior e inferior de plantas, selecionadas ao acaso, distribuídas perpendicularmente ao deslocamento do pulverizador. Após a aplicação do traçador os coletores foram lavados individualmente em solução extratora de ácido nítrico a 1,0 mol L-1. A determinação quantitativa dos depósitos foi realizada com o uso da espectrofotometria de absorção atômica. A assistência de ar junto à barra de pulverização não aumentou a deposição do traçador em folíolos de feijoeiro, aos 48 dias após a emergência da cultura.

SURVIVAL of XANTHOMONAS AXONOPODIS pv. PHASEOLI var. FUSCANS IN COMMON BEAN LEAFLETS on SOIL

The aim of this study was to evaluate the survival of a strain of Xanthomonas axonopodis pv. phaseoli var. fuscans (Xap), resistant to streptomycin sulphate, in common bean leaflets placed on the sod surface and buried at a depth of 10 and 15 cm. Four assays were carried out from November 1998 to December 2000 in Bandeirantes (Paran, Brazil). The leaflets were collected every 15 days, crushed and the dilution-plated on a semi-selective medium. Under mild temperatures and low rainfall, Xap survived for 65 to 180 days in the leaflets on the sod surface, and for 30 to 120 days in those incorporated in the soil, regardless of the depth. When higher rainfall and temperatures occurred, the survival was from 45 to 60 days in the leaflets on the sod surface and from 30 to 45 days in those buried 10 or 15 cm deep.

Efficiency of seed treatment with fungicides in castor bean crop genotypes

With the objective of assessing castor bean genotypes in relation to the influence of seeds treatment on agronomic characteristics and physiological quality of seeds, two experiments were conducted between November, 2005 and March, 2006. In the first study, experimental design used was randomized complete blocks, in factorial scheme (2 x 10), by comparing Lyra hybrid and AL Guarany 2002 cultivar and nine fungicides, including its mixtures and test, with four repetitions. Plant height, stem diameter, medium yield and observation of diseases incidence were evaluated. In the second study, conducted in randomized entirely design, in factorial scheme 2 x 10 with four repetitions, Savana and AL Guarany 2002 genotypes were analyzed, using the same fungicides. Germination, infected and dead seeds, vigor, initial moisture content and simulation of field emergency and seeds sanitary quality were also evaluated. The results obtained point that there is positive influence of seed treatment on agronomic characteristics in castor bean crop, increasing grain yield for AL Guarany cultivar and Lyra hybrid. In the analysis of seed quality, it was observed that seed treatments with fungicides and their mixtures provided superior percentages of germination and vigor, reduced the number of infected and dead seeds and increased seeds emergency for Guarany 2002 cultivar and Savana hybrid.

Germination and early growth of soybean, dry bean and turnip grown under Digitaria horizontalis straw

The study aimed to evaluate the possible inhibitory effects of different concentrations of crabgrass (Digitaria horizontalis Willd.) dry mass incorporated to the soil over the germination and early growth of soybean (Glycine max (L.) Merril.), dry bean (Phaseolus vulgaris L.), and turnip (Raphanus raphanistrum L.). The experimental design adopted was completely random, with four replications where, each one was consisted of a 2.5 L capacity pot. Dry mass of crabgrass at equivalent amounts of 0, 2.5, 5.0 and 10 t ha(-1) were incorporated into the soil. Crops seedling emergence was checked daily, and germination, speed germination index, mean germination time, relative frequency and synchronization index of germination were computed at the final of 10 days. The height and dry mass of plants were evaluated at 35 days after sowing. The incorporation to the soil of D. horizontalis dry mass caused significant reduction of the height and dry weight of soybean, dry bean and turnip, but were not observed consistent influence over the germination of these species.

Nutrient extraction and exportation by castor bean hybrid lyra

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Survival of Curtobacterium flaccumfaciens pv. flaccumfaciens in soil and bean crop debris

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Spray deposition on weeds of common bean crops

A.C.P. Rodrigues-Costa, D. Martins, N.V. Costa, and M.R.R. Pereira. 2011. Spray deposition on weeds of common bean crops. Cien. Inv. Agr. 38(3): 357-365. Weed control failure in common bean (Phaseolus vulgaris L.) production may be related to inappropriate herbicide application techniques. The purpose of this study, therefore, was to evaluate the amount of spray solution deposition that occurred on the weeds, Bidens pilosa L. and Brachiaria plantaginea (Link) Hitch., both within and between rows of common beans. The research was arranged in a randomized block design with four replications. The following 6 spray nozzles were used: flat fan nozzles XR 110015 VS (150 L ha(-1)) and XR 11002 VS (200 L ha(-1)); cone nozzles TX VK 6 (150 L ha(-1)) and TX VK 8 (200 L ha(-1)); and twin flat fan nozzles TJ60 11002 VS (150 L ha(-1)) and TJ60 11002 VS (200 L ha-1). The results showed that the loss of the spray solution on the soil occurred mainly within the bean rows and with a high intensity when using a nozzle spraying 200 L ha(-1). At 30 days after sowing, the TX (150 L ha(-1)) nozzle was the only nozzle that promoted deposits of less than 210.0 mu L g(-1) of dry mass. The spray nozzles showed a good performance in the deposition of the spray solution on the weeds that occurred both within and between the rows. However, for both species there was great variation in individual deposits depending on their location in relationship to the plants.

Feeding substrates to black coconut bunch weevil, Homalinotus coriaceus (Gyllenhal) (Coleoptera: Curculionidae), rearing in laboratory

A broca do cacho do coqueiro, Homalinotus coriaceus (Gyllenhal), é uma praga limitante da produção de coco no Brasil, sendo que tanto as larvas como os adultos provocam a queda das flores femininas e dos frutos imaturos, pela interceptação do fluxo de seiva ou pela alimentação direta nas estruturas reprodutivas. em virtude da escassez de informações sobre sua biologia, realizou-se esse trabalho com o objetivo de desenvolver uma metodologia mais adequada para a criação da praga em laboratório. Foram utilizados os parâmetros biológicos para avaliação e comparação dos sistemas de criação estudados.Toletes de cana-de-açúcar foram utilizados como substrato para alimentação dos adultos coletados no campo e obtenção dos ovos. As larvas foram criadas em três substratos alimentares no Laboratório de Entomologia da Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)-CPATC (Centro de Pesquisa Agropecuária dos Tabuleiros Costeiros), em Aracaju, SE. Os substratos alimentares estudados foram: o mesocarpo do coco, dieta para criação da broca dos citros e dieta para criação da broca do olho do coqueiro, sendo esta a que proporcionou o melhor desenvolvimento larval num menor tempo, com boa viabilidade, maior facilidade no preparo e manutenção.

The nitrogen sufficiency index underlying estimates of nitrogen fertilization requirements of common bean

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Nitrogen fertilization and soil management of winter common bean crop

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Organophosphate resistance in the maize weevil Sitophilus zeamais: Magnitude and behavior

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Black bean (Phaseolus vulgaris L.) as a protective agent against DNA damage in mice

This study was designed to evaluate the toxicogenetic or protective effect of cooked and dehydrated black beans (Phaseolus vulgaris L.) in bone marrow and peripheral blood cells of exposed mice. The frequency of micronuclei detected using the bone marrow erythrocyte micronucleus test and level of DNA lesions detected by the comet assay were chosen as end-points reflecting mutagenic and genotoxic damage, respectively. Initially, Swiss male mice were fed with a 20% black bean diet in order to detect mutagenic and genotoxic activity. However, no increase in the frequency of bone marrow micronucleated polychromatic erythrocytes (MN PCEs) or DNA lesion in leukocytes was observed. In contrast, received diets containing 1, 10 or 20% of black beans, a clear, but not dose-dependent reduction in the frequency of MN PCEs were observed in animals simultaneously treated with cyclophosphamide, an indirect acting mutagen. Similar results were observed in leukocytes by the comet assay. Commercial anthocyanin was also tested in an attempt to identify the bean components responsible for this protective effect. However, instead of being protective, the flavonoid, at the highest dose administered (50 mg/kg bw), induced primary DNA lesion, as detected by the comet assay. These data indicate the importance of food components in preventing genetic damage induced by chemical mutagens, and also reinforce the role of toxicogenetic techniques in protecting human health. (C) 2003 Elsevier Ltd. All rights reserved.

Evaluation of pyraclostrobin and acibenzolar-S-methyl on common bacterial blight of snap bean

Assays were done under greenhouse conditions in order to evaluate the effect of pyraclostrobin (0.0375, 0.0750 and 0.150 mL.L-1) and acibenzolar-S-methyl (ASM) (0.025 g.L-1) in common bacterial blight on leaves of snap beans cultivar Braganca. These chemicals were sprayed at three different times: five days before; five days before + five days after; and five days after leaf inoculation with an isolate of Xanthomonas axonopodis pv. phaseoli. They were determinate the levels of polyphenoloxidase, peroxidase and total soluble proteins on inoculated and non-inoculated leaves of snap beans sprayed with pyraclostrobin (0.075 g.L-1) and ASM (0.025 g.L-1). All concentration of pyraclostrobin and ASM reduced the area under the disease progress curve (AUDPC) on leaves of snap beans, and the least AUDPC value was observed when this products were sprayed five days before + five days after inoculation. Higher levels of polyphenoloxidase, peroxidase and the total soluble proteins were observed on leaves sprayed with pyraclostrobin or ASM.