Avaliação da variabilidade genética em quatro gerações de Eucalyptus urophylla S.T. Blake por meio do marcador molecular RAPD

Molecular markers have gradually replaced morphological markers in population studies. The advantages of molecular markers are the speed and precision of evaluations, mainly for long cycle cultures, where determinate traits can take years to manifest. The principle objectives of this research were to assess variability and genetic distances in four generations of Eucalyptus urophylla and provide data that help with the continued improvement of these materials. The populations can be found at the Experimental Forestry Sciences Station, Anhembi, SP, belonging to the College of Agriculture Luiz de Queiroz of São Paulo University. The initial base population was introduced by seeds collected in indonesia and designated P0 generation. The subsequent segregated generations, derivatives of recombination starting with open pollination, were designated P1, P2, and P3. One hundred and seventy four individual trees representing the four generations were analysed. The RAPD technique allowed the identification of 86 loci that were analysed with the Jaccard Coefficient, generating a genetic similarity matrix, permitting the estimation of genetic distances. The genetic distance of generation PO was 0.3338333, P1 was 0.336824, P2 was 0.40000, and P3 was 0.381093. In percentage terms the genetic distances between individuals grew in relation to base population, being 0.15% for generation P1, 18.93% for P2, and 13.31% for P3. This shows an increase in genetic variability with the advance of the program, despite the selective processes. From this came the belief that the initial base population was resulting from seed collection from isolated trees. These populations, although going through successive selections, had a high cross efficiency through satisfactory pollination, which then permitted genetic variation to increase, the outcome of effective recombination between individuals. Generations P2 and P3 gave a better perspective for the continuance of the improvement program due to the high number of different groups with standard genetic distances of 35%. The selections made between the diverse genetic groups allowed the efficient use of genetic variability evaluation.

Caracterização silvicultural, botânica e avaliação da variabilidade genética por meio do marcador molecular RAPD em um teste de progênies de Eucalyptus urophylla S. T. Blake

Molecular markers have recently been incorporated into genetic improvement programs. They are already considered as powerful tools with several different uses, for instance the monitoring of genetic variability in tree populations. The main objectives of this study were to evaluate genetic variability in Eucalyptus urophylla progenies and together with silvicultural and botanical information, provide assistance to the improvement program. The Eucalypts population is located at the Experimental Forestry Sciences Station, Anhembi, SP, which belongs to the College of Agriculture Luiz de Queiroz. Sixty-nine progenies were analysed representing one individual by family in open pollinated Eucalyptus urophylla trees. The RAPD technique allowed the identification of 72 loci that were analysed using Jaccard's Coefficient generating a genetic similarity matrix to permit estimation of genetic distances. The results obtained showed genetic distance between individuals of 0.40 with 12 groups of genetic variability using a standardised distance of 40%. The progenies showed different bark patterns, allowing the establishment of bark groups. The groups formed based on genetic distances obtained using DNA analysis did not correspond to those based on bark pattern. Genetic selection was simulated in which silvicultural and genetic variability data were linked, thus avoiding excessive variability losses. The simulation of controlled crossings allowed the maximum genetic difference to be obtained linked with height and individual bark roughness.

Variabilidade espacial da estabilidade de agregados e matéria orgânica em solos de relevos diferentes

Soils submitted to the same management system in places with small relief variation manifest different spatial variability on their attributes. The objective of this work was to evaluate the spatial variability of the geometric medium diameter, aggregates in the >2 mm class, aggregates in the 2-1 mm class and organic matter of an Oxisol under culture of the sugarcane. Samplings of the soil in regular intervals of 10 m, in grid form, totaling 100 points, collected in the depths of 0.0-0.2 m and 0.2-0.4 m were made. Data were submitted to descriptive statistics, geostatistics and in sequence to kriging analyzes. Values of the variation coefficient were low for organic matter in the depth of 0.0-0.2 m, mean in the depth of 0.2-0.4 m, high for geometric medium diameter and mean for aggregates in the >2 mm class, aggregates in the 2-1 mm class in all studied depths. The occurrence of space dependence was observed for all the variables, and the largest ones were observed in the depth of 0.0-0.2 m. Small variations in the forms of the relief condition spatial variability differentiated for organic matter and stability of aggregates in the studied depths.

Variabilidade genética intrapopulacional em Myracrodruon urundeuva Fr. All. por marcador AFLP

Levels of genetic variability for in situ and ex situ genetic conservation were estimated in a population of Myracrodruon urundeuva using the PCR (polymerase chain reaction) technique with the AFLP (Amplified fragment-length polymorphism) genetic marker. Seeds for progeny tests were collected from 30 open-pollination trees (matrices) at Paulo de Faria Ecological Station - SP. From this genetic material, three progeny tests were installed on the Teaching and Research Farm of Ilha Solteira Faculty of Engineering - University of São Paulo State (UNESP), which is located in Selvlria - MS, Brazil. The analysis by genetic marker was conducted with three combinations of different starters EcoRl-Msel, resulting in a total number of 137 polymorphic bands, thus forming a table of binary data. These data were used for the analysis of genetic divergence and distance between progenies. High levels of genetic divergence were observed among families. Based on the Analysis of Molecular Variance (AMOVA), it was shown that 16.2% of genetic diversity is found among progenies and 83.8% within progenies, which suggests deviances of random matings. The grouping of progenies, based on genetic distances, suggests that progenies deriving from trees which are close to each other tend to be more similar. This, in turn, indicates that the population originating the seeds may be genetically structured.

Variabilidade espacial das propriedades físicas e químicas do solo em áreas intensamente cultivadas

The spatial variability of physical and chemical properties of soil were evaluated to provide subsidies for management of the agricultural input. The chemical variables: P, organic matter (OM), K, Ca, Mg, pH, CEC and base saturation (BS); and physical variables: sand and clay were analysed. Soil samples were collected at two depths (0-0.2 and 0.6-0.8 m) located at irregular mesh of sampling in the region of Monte Alto, in a Yellow-Red Podzol (Alfissolo) (PVA), under different managements, resulting in 88 points in 1465 ha of total area; and at the region of Jaboticabal in a Red Latosol (LV) cultivated with sugarcane, resulting in 128 points in 2597 ha of total area. The chemical and physical soil properties studied showed spatial dependence, except CEC in 0.6-0.8 m layer for LV; Ca and clay at 0-0.2 m layer and P, OM, K, Mg, sand and clay in 0.6-0.8 m layer for PVA. The chemical and physical variables studied were adjusted to spherical and exponential models and some of them showed semivariogram without defined structure. The PVA showed low spatial continuity of chemical and physical properties, mainly in 0.6-0.8 m layer, which suffers less antropic influence. The LV soil showed more homogenous zones of fertility and granulometric composition.

Variabilidade espacial de perdas de solo, do potencial natural e risco de erosão em áreas intensamente cultivadas

The objective of this work was to identify the spatial variability of the natural erosion potential, soil loss and erosion risk in two intensely cultivated areas, in order to assess the erosion occurrence patterns. The soil of the area located at Monte Alto, São Paulo state, was classified as Paleudalf (PVA) with moderately slope, with different managements. The soil of the area located at Jaboticabal, São Paulo state, was classified as Haplortox(LV) with gentle slope and cultivated with sugarcane. A irregular grid was imposed on the experimental areas. Soil samples were obtained from 0-0.2 m depth at each grid point: 88 samples in Monte Alto area (1465 ha) and 128 samples at Jaboticabal area (2597 ha). In order to obtain the values of the studied variables USLE was applied at each grid point. Descriptive statistics were calculated, and geoestatistical analyses were performed for defining semivariograms. Kriging techniques to develop map showing spatial patterns in variability of selected soil attributes were used. All variables showed spatial dependence. The PVA soil showed higher erosion risk due to the slope and atual management compared to the soil LV.

Variabilidade clínica na determinação da dose tóxica oral em intoxicação experimental por fluoroacetato de sódio em gatos

The sodium monofluoroacetate (FAC) or compound 1080 is a potent rodenticide used for a rodents and vertebrate pest control. It was prohibited in many countries because of its high toxicity, but in Brazil exist evidences of ilegal use causing the intoxication in children and domestic animals. The fluoroacetate metabolite, fluorocitric acid, blocks body energy production by inhibit the Krebs cycle, resulting in neurological and cardiacs signs. In the present study, four group of oral toxic dosis of the FAC were compared in cats. The best oral toxic dose for clinical signs presentation, without cause acute lethality, was 0,45mg/kg. The clinical variability was dosis dependent and its intensity, in crescent order, was: light signs (dose 1: 0,3mg/kg), light to moderate (dose 2: 0,4mg/kg), moderate to severe (dose 3: 0,45mg/ kg) and severe (dose 4: 0,5mg/kg). There was individual clinical variability between animals that received the same oral toxic dose.

Variabilidade genética na porção codificadora para a proteína capsidial do Lettuce big-vein associated virus e Mirafiori lettuce big-vein virus provenientes de alface no Brasil

Lettuce big vein associated virus (LBVaV) and Mirafiori lettuce big vein virus (MLBVV) have been found in mixed infection in Brazil causing the lettuce big vein disease. Analysis of part of the coat protein (CP) gene of Brazilian isolates of LBVaV collected from lettuce, showed at least 93% amino acid sequence identity with other LBVaV isolates. Genetic diversity among MLBVV CP sequences was higher when compared to LBVaV CP sequences, with amino acid sequence identity ranging between 91% to 100%. Brazilian isolates of MLBVV belong to subgroup A, with one RsaI restriction site on the coat protein gene. There is no indication for a possible geografical origin for the Brazilian isolates of LBVaV and MLBVV.

Variabilidade temporal e mapeamento dos dados climáticos de Botucatu-SP

O trabalho teve objetivo estudar a variabilidade temporal da temperatura do ar, precipitação pluviométrica e umidade relativa do ar na cidade de Botucatu-SP, Brasil, utilizando técnicas geoestatísticas. Os dados de precipitação pluviométrica, temperatura do ar e umidade relativa do ar utilizados no presente estudo são provenientes da Estação Meteorológica da Fazenda Lageado, da Faculdade de Ciências Agronômicas-UNESP. As observações foram realizadas no período de 1988 a 2007, referem-se ao total mensal de precipitação pluvial expressa em altura de lâmina d'água (mm), médias mensais de temperatura em ºC e umidade relativa em %. Os dados foram avaliados por meio da estatística clássica e geoestatística. As variáveis climáticas tiveram sua dependência verificada por variogramas, apresentando dependência temporal maior que 76%. A série temporal de umidade relativa do ar foi a que apresentou maior alcance (8,67 meses) e, conseqüentemente, maior estabilidade climática. O conhecimento da distribuição temporal das variáveis climáticas é importante para o estudo e realização do zoneamento agroclimático, bem como para o dimensionamento do sistema de irrigação das culturas.

Variabilidade temporal e meridional de estimativas de Satélite da concentração de clorofila superficial na plataforma continental brasileira

Forecast of biological consequences of climate changes depend on both long-term observations and the establishment of carbon budgets within pelagic ecosystems, including the assessment of biomasses and activities of all players in the global carbon cycle. Approximately 25% of oceanic primary happen over continental shelves, so these are important sites for studies of global carbon dynamics. The Brazilian Continental Shelf (BCS) has sparse and non-systematic in situ information on phytoplankton biomass, making products derived from ocean color remote sensing extremely valuable. This work analyzes chlorophyll concentration (Chl) estimated from four ocean color sensors (CZCS, OCTS, SeaWiFS and MODIS) over the BCS, to compare Chl and annual cycles meridionally. Also, useful complementary ocean color variables are presented. Chl gradients increased from the central region towards north and south, limited by estuarine plumes of Amazon and La Plata rivers, and clear annual Chl cycles appear in most areas. In southern and central areas, annual cycles show strong seasonal variability while interannual and long-term variability are equally important in the remaining areas. This is the first comparative evaluation of the Chl over the BCS and will aid the understanding of its long-term variability; essential initial step for discussions of climate changes.

Variabilidade espacial do estado nutricional do cafeeiro canephora visando o manejo localizado

Mapping the plant nutritional condition allows viewing different regions in a cropping area, providing the producers with different criteria to use foliar and soil fertilization. The aim of this study was to evaluate the spatial variability of the nutritional condition of canephora coffee (Coffea canephora Pierre ex Froehner) regarding the site specific management of foliar and soil fertilization. In a one hectare area 60 georeferenced points were sampled at irregular intervals. There were five plants in each sampled point; two pairs of leaves were removed from the lateral branches (3 rd and 4 th pairs from extremity to the basis) in the cardinal points of each plant, counting up 40 leaves per point. The foliar samples were chemically analyzed for the following nutrients: N, P, K, Ca, Mg, S, B, Cu and Zn. The same pattern of spatial dependence was presented with adjustment for K and B. Except for N and P, which presented random distribution, the other nutrients presented mild to severe spatial dependence justifying the geostatistical data analysis for making maps for differential and located, foliar and soil fertilizer application in coffee crop.

Variabilidade espacial de atributos químicos do solo cultivado com café arábica (Coffea arabica L.) sob diferentes manejos

The objective of this work was to assess the spatial variability of the chemical attributes of two coffee areas, managed in conventional and organic crop systems, and to calculate the percent of variation between them. In each area, a 40-point-mesh was sampled at 0-0.10 m and 0.10-0.20 m layers, within the crown projection, for pH, SB, K, P, Ca and Mg analysis. The data were analyzed through descriptive statistics and geostatistics. From the soil chemical attributes map, the percent of variation between the systems' chemical attributes was determined by GIS algebraic operations. The results show that the soil chemical attributes present a spatial dependence in both systems and layers. Analysis of the soil chemical attributes showed less spatial variability in the organic system, in relation to the conventional, indicating homogeneous zones for different fertilizer applications. The percent of variation of the chemical attributes in the conventional system, in relation to the organic, at 0-0.10 m and 0.10-0.20m layers are 54.80% and 35.61%, respectively.

Modelo agrometeorológico na estimativa da produtividade de duas variedades de café arábica considerando a variabilidade espacial

The purpose of this study was to estimate the varieties of coffee Arabic Catucáand Catuaí productivity, in Zona da Mata, Minas Gerais, through agrometeorological mathematical models, considering the spatial variability of productivity in the area. The samplings were georrefered building an irregular grid, totalizing 50 samplings per area. After that, geostatic analysis was made to quantify spatial dependence degree of the real values and the estimated productivity. According to the classification, the models superestimated the productivity for the two varieties.

Variabilidade dos atributos de um latossolo vermelho sob plantio direto no cerrado brasileiro e produtividade da soja

The spatial variability of several soil attributes (bulk density, penetration resistance, water content, organic matter content and pH) as well as soybean yield have been assessed during the 2007/08 growing season, in Selviria (MS) in a Hapludox (Typic Acrustox), under no tillage. The objectives were to assess the spatial variability of soil and plant parameters at the small plot scale and to select the best soil attribute explaining most the variability of agricultural productivity. Soil and plant were sampled on a grid with 121 points within a plot of 25,600 m 2 in area and slope of 0.025 mm -1 slope. Medium and low coefficients of variation were obtained for most of the studied soil attributes as expected, due to the homogenizing effect of the no-till system on the soil physical environment. From the standpoint of linear regression and spatial pattern of variability, productivity of soybeans could be explained according to the hydrogen potential (pH). Results are discussed taken into account that the soybean crop in no-tillage is widely used in crop-livestock integration on the national scene.

Variabilidade espacial na produção da alface em ambiente protegido

Este artigo mostra a dinâmica da água após ser aplicada sobre a superfície de um solo cultivado com alface, num sistema fechado de um ambiente protegido. A aplicação de água se deu por meio de um sistema de irrigação por gotejamento, com o CUD ( Coeficiente de Uniformidade de Distribuição ) igual a 97,06 %, o que garantiu uma ótima uniformidade de distribuição, desconsiderando como possíveis causas das variações de umidade do solo, a aplicação de lâminas variadas. Fazendo uso de técnicas de geoestatística, interpolando sessenta pontos amostrais de evaporação, umidade do solo e produtividade da alface, com as mesmas sessenta coordenadas para os três atributos, verificou-se que a umidade do solo, mesmo momentos após a irrigação, não permanece uniforme ao longo do sistema de irrigação, constatando-se que a evaporação local exerce influência de forma predominante sobre a dinâmica da água na superfície do solo. Concluiu-se que as regiões no interior da estufa de maiores evaporações, foram as de maiores variações de umidade do solo e na produção de massa verde da alface.