Development of paper-based color test-strip for drug detection in aquatic environment: Application to oxytetracycline

The wide use of antibiotics in aquaculture has led to the emergence of resistant microbial species. It should be avoided/minimized by controlling the amount of drug employed in fish farming. For this purpose, the present work proposes test-strip papers aiming at the detection/semi-quantitative determination of organic drugs by visual comparison of color changes, in a similar analytical procedure to that of pH monitoring by universal pH paper. This is done by establishing suitable chemical changes upon cellulose, attributing the paper the ability to react with the organic drug and to produce a color change. Quantitative data is also enabled by taking a picture and applying a suitable mathematical treatment to the color coordinates given by the HSL system used by windows. As proof of concept, this approach was applied to oxytetracycline (OXY), one of the antibiotics frequently used in aquaculture. A bottom-up modification of paper was established, starting by the reaction of the glucose moieties on the paper with 3-triethoxysilylpropylamine (APTES). The so-formed amine layer allowed binding to a metal ion by coordination chemistry, while the metal ion reacted after with the drug to produce a colored compound. The most suitable metals to carry out such modification were selected by bulk studies, and the several stages of the paper modification were optimized to produce an intense color change against the concentration of the drug. The paper strips were applied to the analysis of spiked environmental water, allowing a quantitative determination for OXY concentrations as low as 30 ng/mL. In general, this work provided a simple, method to screen and discriminate tetracycline drugs, in aquaculture, being a promising tool for local, quick and cheap monitoring of drugs.

O poder dos media sociais no modelo de comunicação da World Cruising Club

O presente relatório descreve o trabalho desenvolvido durante o estágio na WCC (World Cruising Club), no departamento de Rally Control, que acompanha os diversos eventos da agência. Os serviços da empresa oferecem uma série de regatas à volta do mundo que dão, a quem participa, uma oportunidade de turismo diferente que inclui navegar, conhecer novos povos, países e culturas assim como muita diversão. O departamento do qual fiz parte, para além de toda organização do evento em cada destino visitado, desenvolve ainda projetos na área dos novos media com o intuito, não só, da promoção da empresa, mas também de mostrar às família e amigos dos participantes, que se encontram longe, onde os mesmos se encontram, as diferentes atividades e descobertas feitas em cada destino visitado. Procurou-se compreender quais as melhores estratégias de comunicação para a promoção desta empresa através dos novos meios de comunicação, recorrendo a conceitos do marketing digital, nomeadamente a promoção a partir das plataformas Facebook e Twitter.

El desarrollo de un puerto pesquero modelo en el departamento de Lambayeque. Conferencia dada el día 15 de enero 1964 en el Club Lambayeque, Lima

Exposición presentada sobre el desarrollo de un puerto pesquero donde se han estudiado las condiciones y las características de la costa, conociendo sus riquezas ictiológicas de la zona por la pesca de varios cientos de años.

Guide de route... / Touring-club de France

1938.

Guide de route... (Touring-Club de France)

Périodicité : Annuel

Guide de route... / Touring-club de France

1937.

Beleuse, nageur [du Sporting Club Parisien] : [photographie de presse] / [Agence Rol]

Référence bibliographique : Rol, 58555

Beleuse, nageur [du Sporting Club Parisien] : [photographie de presse] / [Agence Rol]

Référence bibliographique : Rol, 58556

d'Arsonval [professeur de tennis au Sporting club de Paris] : [photographie de presse] / [Agence Rol]

Référence bibliographique : Rol, 58314

Vitry, 14/3/20, asson [football association] équipe C.A. [Club Athlétique] de Vitry : [photographie de presse] / [Agence Rol]

Référence bibliographique : Rol, 58502

Vitry, 14/3/20, asson [football association], équipe C.A. [Club Athlétique] de Vitry : [photographie de presse] / [Agence Rol]

Référence bibliographique : Rol, 58503

[Portrait d'] Yvonne Degraine [nageuse, avec le maillot du club des Mouettes] : [photographie de presse] / [Agence Rol]

Référence bibliographique : Rol, 58627

Yvonne Degraine [nageuse, avec le maillot du club des Mouettes] : [photographie de presse] / [Agence Rol]

Référence bibliographique : Rol, 58628

2/5/20, [stade Bergeyre], championnat de Paris, [football] association, Red Star contre R.C.F. [Racing Club de France] : [photographie de presse] / [Agence Rol]

Référence bibliographique : Rol, 59140

2/5/20, [stade Bergeyre], championnat de Paris, [football] association, Red Star contre R.C.F. [Racing Club de France] : [photographie de presse] / [Agence Rol]

Référence bibliographique : Rol, 59141