Mappa subtilis, Orbis Marianus, Fasciculus Dunsius, speculum Scoticum ferme innumera praestantisimorum virorum detegens vocitans eiusdem examlien elogia venerabilis serui Dei Ioannis Dunsij Scoti ... /

Mode of access: Internet.

Repertorium locupletissimum tam librorum sententiarum, quam quolibetorum doct. subtilis Iohannis Duns Scoti : olim à doctissimo P. M. Hieronymo de Ferrariis Fantono.

Mode of access: Internet.

Eximii doctor magistri Nicolai de Orbellis ..., Super sententias compendiu[m] singulare elega[n]tiora doctoris subtilis dicta summarium complectens : quod nunc dudum multis viciatum erroribus castigatissime recognitium noue extat

Mode of access: Internet.

Mappula scotica et augustiniana subtilis, ac mariani doctoris conclusiones : ex scripto oxoniensi decerptas, divi Augustini, primi ecclesiae doctoris, aureis sententiis cohaerescens /

Mode of access: Internet.

Liber primus, [-secundus] correspondens primo [-secundo] Lib. sentent. Scoti doctoris subtilis : discursus praedicabiles theologici speculativi... per quos habetur expositio primi v secundi [& tertij] Cantici Canticorum Salomonis iuxta doctrinem et ordinem quaestionum Scoti in primo [& 2.] libro Sententiarum /

En p. [9] de tomo 1 esc. calc.

Primus [-quartus] liber doctoris subtilis fratris Joannis Duns Scoti ordinis Minorum super Sententias : titulus conueniens doctrine atq[ue] auctori huius primi Mauritio inuentore ...

Mode of access: Internet.

Tractatus de Sacramentis in genere et aliquibus in specie, in doctrina subtilis doctoris Scoti /

Mode of access: Internet.

Conciliatio dilucida omnium controuersiarum, quae in doctrina duorum summorum Theologorum S. Thomae, [et] subtilis Ioannis Scoti passim leguntur ... /

Edit16,

Conciliatio dilucida omnium controuersiarum quae in doctrina duorum summorum theologorum S, Thomae, [et] subtilis Ioannis Scoti passi leguntur ... /

Mode of access: Internet.

Theologia moralis ad mentem D.D. Seraphici [et] subtilis concinnata et in disputationes vigintiocto distributa /

Mode of access: Internet.

Investigation of the roles of CheD in the Bacillus subtilis chemotactic signal transduction

Abstract The two-component based chemotaxis signal transduction system allows flagellated bacteria to sense their surrounding chemical environment and move towards more favorable conditions. The attractant signals can be sensed by transmembrane chemoreceptors, and then transmitted to the histidine kinase CheA. Once activated, CheA interacts with the response regulator CheY through phosphorelay, which causes a change in the rotation of the flagella. The direction of flagella rotation determines whether a cell swims straight or just tumbles. Cells also need adaptation to respond to a change in chemical concentrations, and return to their prestimulated level. Adaptation in the B. subtilis chemotaxis system is achieved by three coordinated systems: the methylation system, the CheC/CheD/CheY-p system and the CheV system. CheD, the previously identified receptor deamidase, was shown to be critical to the ability of B. subtilis to perform chemotaxis and is the main focus of this study. This study started from characterization of the enzymatic mechanism of CheD. Results showed that CheD deamidase uses a cysteine hydrolase mechanism. The catalytic triad consisting of Cys33-His50-Thr27, and Ser27 is essential for receptor recognition and binding. In addition, in this study CheC was found to inhibit CheD’s deamidase activity. Through mutant screening, Phe102 on CheD was found to be the essential site to interact with CheC. Furthermore, the CheD/CheC interaction is necessary for the robust chemotaxis in vivo as demonstrated by the cheD (F102E) mutant, which lacks the ability to swim on swarm plates. Despite its deamidase activity, we hypothesized that CheD’s main role is its involvement in the CheD-CheC-CheY-p negative feedback pathway during adaptation. In particular, CheD is likely to help stabilize the transient kinase-activating state through binding to receptors. When CheY-p level is increased, CheC-CheY-p complex may attract CheD away from receptors. In this study, CheC-CheD binding kinetics with CheY or CheYp presence was successfully obtained by a series of SPR experiments. The increased affinity of CheD for CheC in presence of CheYp but not CheY makes likely the hypothesis that CheC-CheD-CheY interact as part of a negative feedback pathway during adaptation. Last, the interaction between CheD and chemoreceptor McpC was studied in order to better understand the role of CheD in adaptation. Results showed that Q304 and Q305 on McpC are essential to recruit CheD. Additionally, the reduced levels of CheD in mcpC (Q304A) or (Q305A) mutants suggested that the dynamic interaction between CheD and receptors is vital to maintain the normal CheD level. These findings suggest more complicated roles of CheD than its previously identified function as a receptor deamidase, and will lead to a clearer picture of the coordination of the three adaptational systems in the B. subtilis chemotactic sensory transduction system.

Helicase binding to DnaI exposes a cryptic DNA-binding site during helicase loading in Bacillus subtilis

The Bacillus subtilis DnaI, DnaB and DnaD proteins load the replicative ring helicase DnaC onto DNA during priming of DNA replication. Here we show that DnaI consists of a C-terminal domain (Cd) with ATPase and DNA-binding activities and an N-terminal domain (Nd) that interacts with the replicative ring helicase. A Zn2+-binding module mediates the interaction with the helicase and C67, C70 and H84 are involved in the coordination of the Zn2+. DnaI binds ATP and exhibits ATPase activity that is not stimulated by ssDNA, because the DNA-binding site on Cd is masked by Nd. The ATPase activity resides on the Cd domain and when detached from the Nd domain, it becomes sensitive to stimulation by ssDNA because its cryptic DNA-binding site is exposed. Therefore, Nd acts as a molecular 'switch' regulating access to the ssDNA binding site on Cd, in response to binding of the helicase. DnaI is sufficient to load the replicative helicase from a complex with six DnaI molecules, so there is no requirement for a dual helicase loader system.

The Bacillus subtilis primosomal protein DnaD untwists supercoiled DNA

The essential Bacillus subtilis DnaD and DnaB proteins have been implicated in the initiation of DNA replication. Recently, DNA remodeling activities associated with both proteins were discovered that could provide a link between global or local nucleoid remodeling and initiation of replication. DnaD forms scaffolds and opens up supercoiled plasmids without nicking to form open circular complexes, while DnaB acts as a lateral compaction protein. Here we show that DnaD-mediated opening of supercoiled plasmids is accompanied by significant untwisting of DNA. The net result is the conversion of writhe (Wr) into negative twist (Tw), thus maintaining the linking number (Lk) constant. These changes in supercoiling will reduce the considerable energy required to open up closed circular plectonemic DNA and may be significant in the priming of DNA replication. By comparison, DnaB does not affect significantly the supercoiling of plasmids. Binding of the DnaD C-terminal domain (Cd) to DNA is not sufficient to convert Wr into negative Tw, implying that the formation of scaffolds is essential for duplex untwisting. Overall, our data suggest that the topological effects of the two proteins on supercoiled DNA are different; DnaD opens up, untwists and converts plectonemic DNA to a more paranemic form, whereas DnaB does not affect supercoiling significantly and condenses DNA only via its lateral compaction activity. The significance of these findings in the initiation of DNA replication is discussed.

Análise da viabilidade comercial de produtos à base de Bacillus subtilis e Bacillus pumilus para o controle de fitopatógenos no Brasil.

2009