Investigação soroepidemiológica de leptospirose canina na área territorial urbana de Botucatu, São Paulo, Brasil

Leptospirosis was seroepidemiologically investigated in 775 dogs from Botucatu, state of Sao Paulo, in blood samples collected during the annual anti-rabies vaccination campaign. The samples were collected from 14 out of the 45 vaccination stations geographically distributed in five different municipal districts, with a territorial urban area of 31Km2. Samples were taken from 449 males and 326 females; 564 were of undetermined breed, and 211 were pedigree animals; ages ranged from 3 months to 20 years. Diagnosis included the microscopic serum agglutination tests using 12 Leptospira spp serovars. Serological results and epidemiological variables were compared using Goodman and chi-square tests, with χ=0.05. There were 119 (15.3%) positive samples for 11 serovars; the most important was canicola with 48 (40.3%), followed by pyrogenes 41 (34.5%). Statistical analysis showed significant difference for: breed, 17.7% positive in undetermined breeds and 9.0% in pedigrees; and sex, 18.4% positive in males and 11.0% in females. There was no statistically significant difference in relation to age with uniform distribution of positives.

Bovine abortion associated with Neospora caninum: Diagnosis and epidemiological aspects of a dairy cattle herd in the northeast region of São Paulo state, Brazil

The objective of this study was to report the presence of Neospora caninum-associated abortion in bovines at a farm in the northeast region of São Paulo State. In January 2010, it was sent to the Department of Pathology, UNESP-Jaboticabal, a bovine fetus with an estimated age of seven months, which was natural of a dairy farm with 300 animals and an average daily production of 3,000 liters of milk, nearly 20 liters per cow. The animals were vaccinated against rabies, foot and mouth disease, carbuncle, brucellosis, leptospirosis, bovine herpes virus type I and bovine viral diarrhea virus. The herd consisted of purebred Holstein animals, Jersey, and mostly by crossbred animals 7-8 (gir x holstein). During necropsy, samples of the serosanguineous liquid present at the thoracic cavity and the heart of the fetus were collected for the detection of anti-Neospora caninum antibodies through Indirect Immunofluorescence Assay (IFA). Fragments of brain, cerebellum, tongue, liver, heart and kidneys were collected for the execution of histopathology (HP), immunohistochemistry (IHC) and Polymerase Chain Reaction. In order that IFA could be performed, the owner was requested blood samples without anticoagulants of the mother and other cows in the farm, with or without a history of abortion. At necropsy, it was verified a severe autolysis of the fetus. The serology of the fetus was 1:25, while the serology of the mother was 1:3,200. At HP, it was observed discrete multifocal non-suppurative encephalomyelitis characterized by gliosis and mononuclear inflammatory infiltration associated with cellular debris. DNA amplification of N. caninum was positive in fragments of brain, tongue, cerebellum, heart and kidneys. At IHC, it has been observed immunoreactivity to a cyst located in the tongue. The owner reported that his herd showed endemic episodes of abortion, while 27.69% (18/65) of the 65 animals sampled were seropositive. Although it has not been a significant difference (p>0.05), a higher seropositivity was observed in animals with a history of abortion (10/26) 38.46%, in comparison with animals without previous abortion (8/39) 20.51%. These findings show that the abortion under study was provoked by the protozoan N. caninum, while this is the first report concerning cattle in the northeast region of São Paulo State.

Caracterização de agravos causados por cães e gatos a seres humanos no município de jaboticabal, são paulo, durante o período de 2000 A 2009

The lack of proper control of dogs and cats population and irresponsible pet ownership are factors that favor animal attack and pose serious public health concern. The aim of the present study was to characterize the injuries caused by dogs and cats to humans in the municipality of Jaboticabal, between the years 2000 and 2009, with a view to demonstrate the importance of aggression control in reducing both the risk for rabies transmission and the number of post exposure prophylaxis administration. A retrospective and descriptive study was conducted. 3,972 out of 4,149 records of patients who sought the public health service after being attacked by an animal were analyzed. Dogs were the species that most caused injuries. In the moment of the attack, the doǵs clinical status was declared as healthy in 81.8% of records; and in 81.1% of cases this status were maintained throughout the entire observation period of the aggressor. Most of injuries were characterized by bites in hands or feet, with multiple and superficial wounds. The number of injuries was high, therefore it is crucial to implement strategies to reduce injuries caused by animal attack through health promotion, educational activities and strengthening of integration of public health and veterinary services so that the decision to institute post-exposure prophylaxis is taken with criteria and safety.

Estimativa da febre maculosa em cães para a vigilância e motitoramento da antropozoonose no municipio de Botucatu, no Estado de São Paulo

Pós-graduação em Medicina Veterinária - FMVZ

Caracterização antigênica e genotípica de isolados do vírus rábico, de quirópteros da cidade de Botucatu-SP e região

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Análise filogenética correlacionada com a distribuição do vírus rábico em quirópteros naturalmente infectados

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação da população de cães e gatos com proprietário, e do nível de conhecimento sobre a raiva e posse responsável em duas áreas contrastantes da cidade de Jaboticabal, São Paulo

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Eficácia de uma vacina comercial contra a raiva frente a desafios com amostras de vírus de campo comparados ao desafio padrão no teste NIH

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação dos registros de profilaxia anti-rábica humana pós-exposição no Município de Jaboticabal, são Paulo, no período de 2000 a 2006

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

A aplicação de um inquérito populacional como auxiliar no planejamento da educação em saúde, promoção da posse responsável e controle da raiva nos municípios de Botucatu e Lençóis Paulista - SP

Pós-graduação em Medicina Veterinária - FMVZ

Dinâmica populacional canina na área urbana do município de Araçatuba, SP, no período de 1994 a 2004

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Levantamento da fauna de morcegos (Mammalia, Chiroptera) e ocorrência de vírus rábico na Região de Araçatuba - São Paulo, Brasil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Comparação entre as técnicas de RT-PCR e inoculação intracerebral em camundongos para detecção do vírus da raiva em amostras mantidas por longos períodos em diferentes estados de conservação

Pós-graduação em Ciência Animal - FMVA

Avaliação do método de sucção de encéfalo de morcegos (Chiroptera, Mammalia) para diagnóstico da raiva

Pós-graduação em Ciência Animal - FMVA

Avaliação da imunidade ativa de cães primovacinados (Fuenzalida & Palácios) no intervalo de campanhas anuais de vacinação contra a raiva no município de Botucatu/SP

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)