Effect of chitosan-Aloe vera coating on postharvest quality of blueberry (Vaccinium corymbosum) fruit

The present study was carried out to evaluate the effect of chitosan-based edible coatings with Aloe vera extract on the postharvest blueberry fruit quality during storage at 5 °C. Firstly, A. vera fractions (pulp and liquid) were extracted from leaves and evaluated in terms of antifungal and antioxidant capacities. The choice of the most adequate chitosan and A. vera fraction concentrations to be incorporated in coating formulation was made based on the wettability of the corresponding coating solutions. Coatings with 0.5% (w/v) chitosan + 0.5% (w/v) glycerol + 0.1% (w/v) Tween 80 + 0.5% (v/v) A. vera liquid fraction presented the best characteristics to uniformly coat blueberry surface. Physico-chemical (i.e., titratable acidity, pH, weight loss) and microbiological analyses of coated blueberries (non-inoculated or artificially inoculated with Botrytis cinerea) were performed during 25 d. Microbiological growth and water loss levels were approximately reduced by 50% and 42%, respectively, in coated blueberries after 25 d compared to uncoated blueberries. After 15 d, weight loss values were 6.2% and 3.7% for uncoated and chitosanA. vera coated blueberries, respectively. Uncoated fruits presented mold contamination after 2 d of storage (2.0 ± 0.32 log CFU g1), whilst fruits with chitosan-based coatings with A. vera presented mold contamination only after 9 d of storage (1.3 ± 0.35 log CFU g1). Overall, coatings developed in this study extend blueberries shelf-life for about 5 d, demonstrating for the first time that the combination of chitosan and A. vera liquid fraction as edible coating materials has great potential in expanding the shelf-life of fruits.

A novel hanging spherical drop system for the generation of cellular spheroids and high throughput combinatorial drug screening

We propose a novel hanging spherical drop system for anchoring arrays of droplets of cell suspension based on the use of biomimetic superhydrophobic flat substrates, with controlled positional adhesion and minimum contact with a solid substrate. By facing down the platform, it was possible to generate independent spheroid bodies in a high throughput manner, in order to mimic in vivo tumour models on the lab-on-chip scale. To validate this system for drug screening purposes, the toxicity of the anti-cancer drug doxorubicin in cell spheroids was tested and compared to cells in 2D culture. The advantages presented by this platform, such as feasibility of the system and the ability to control the size uniformity of the spheroid, emphasize its potential to be used as a new low cost toolbox for high-throughput drug screening and in cell or tissue engineering.

Electronic tongue: a versatile tool for mineral and fruit-flavored waters recognition

Natural mineral waters (still), effervescent natural mineral waters (sparkling) and aromatized waters with fruit-flavors (still or sparkling) are an emerging market. In this work, the capability of a potentiometric electronic tongue, comprised with lipid polymeric membranes, to quantitatively estimate routinely quality physicochemical parameters (pH and conductivity) as well as to qualitatively classify water samples according to the type of water was evaluated. The study showed that a linear discriminant model, based on 21 sensors selected by the simulated annealing algorithm, could correctly classify 100 % of the water samples (leave-one out cross-validation). This potential was further demonstrated by applying a repeated K-fold cross-validation (guaranteeing that at least 15 % of independent samples were only used for internal-validation) for which 96 % of correct classifications were attained. The satisfactory recognition performance of the E-tongue could be attributed to the pH, conductivity, sugars and organic acids contents of the studied waters, which turned out in significant differences of sweetness perception indexes and total acid flavor. Moreover, the E-tongue combined with multivariate linear regression models, based on sub-sets of sensors selected by the simulated annealing algorithm, could accurately estimate waters pH (25 sensors: R 2 equal to 0.99 and 0.97 for leave-one-out or repeated K-folds cross-validation) and conductivity (23 sensors: R 2 equal to 0.997 and 0.99 for leave-one-out or repeated K-folds cross-validation). So, the overall satisfactory results achieved, allow envisaging a potential future application of electronic tongue devices for bottled water analysis and classification.

Estrategia de vida implementada por un grupo de productores frutihortícolas del cinturón verde de la ciudad de Córdoba. Life strategies implemented by a group of fruit-horticultural producers from the green belt of the city of Córdoba.

Este proyecto estudiará las estrategias de vida implementadas por familias productoras fruti hortícolas que viven en Colonia Tirolesa y Chacra de la Merced, lugares que forman parte del cinturón verde de la Ciudad de Córdoba; y su relación con los procesos de diferenciación social, asociados a cambios productivos, económicos, culturales y sociales que están condicionados por el modelo de expansión capitalista. A partir del análisis del capital económico, social, cultural y simbólico se identificará en un contexto rural las motivaciones, logicas productivas y conocimientos agrícolas que incidieron en las decisiones asumidas ante problematicas emergentes, tales como limitaciones para adquirir nuevos paquetes tecnológicos, el impacto ambiental causados por la no conservación y sustentabilidad de los recursos y las consecuencias sociales vinculadas a los laxos de familia, el trabajo y la calidad de vida.Se plantean las siguientes hipótesis: 1)Algunas familias productoras implementaron estrategias de vida que se relacionan con criterios de autosustentabilidad, conocimientos agrícolas tradicionales y pautas culturales.2) Los nuevos espacios productivos no son compatibles con agroecosistemas sustentables. 3) Las nuevas tecnologías causaron impactos en la actividad productiva e incidieron en la economía, calidad de vida y vinculos familiares. 4)El modo de producción familiar fue reemplazado por el modo de producción capitalista lo que incidió en la producción agrícola y en la conservación de los recursos.El objetivo principal es descubrir las estrategias de vida implementadas por un grupo de familias, trabajadoras fruti hortícolas, en respuesta a las transformaciones productivas, tecnológicas y socio económicas impuestas por el modelo de acumulación vigente.Para ello se caracterizará los ambitos productivos de trabajo a campo, identificando la disponibilidad de capital y los conocimientos técnicos y de manejo. También se analizarán las pautas y valores culturales, el impacto de las nuevas tecnologías y la incidencia social, vinculada a los cambios en los modos de producción.El abordaje será de tipo cualitativo, recavando información para elaborar una descripción detallada de las estrategias de vida adoptadas a partir de la década de los noventa. Se tomará como unidad de análisis a las familias que trabajaron o trabajan en la producción de hortalizas o frutales. Se realizarán combinaciones entre procedimientos Tipológicos a Priori; Históricos Comparativos y casos Unitarios. Se analizará las normativas vigentes en relación al uso y calidad del agua y del suelo; también imágenes satelitales para analizar los procesos de cambio en el uso del territorio y del recurso suelo. Se pretende realizar un aporte relacionado con el conocimiento de: la sustentabilidad de los sistemas productivos, la pobreza y los modos de vida de los productores fruti hortícolas que persistencia en ambientes degradados; en una franja intermedia entre el campo y la ciudad.

Carlos Chagas Discoveries as a Drop Back to Scientific Construction of Chronic Chagas Heart Disease

Abstract The scientific construction of chronic Chagas heart disease (CCHD) started in 1910 when Carlos Chagas highlighted the presence of cardiac arrhythmia during physical examination of patients with chronic Chagas disease, and described a case of heart failure associated with myocardial inflammation and nests of parasites at autopsy. He described sudden cardiac death associated with arrhythmias in 1911, and its association with complete AV block detected by Jacquet's polygraph as Chagas reported in 1912. Chagas showed the presence of myocardial fibrosis underlying the clinical picture of CCHD in 1916, he presented a full characterization of the clinical aspects of CCHD in 1922. In 1928, Chagas detected fibrosis of the conductive system, and pointed out the presence of marked cardiomegaly at the chest X-Ray associated with minimal symptomatology. The use of serological reaction to diagnose CCHD was put into clinical practice in 1936, after Chagas' death, which along with the 12-lead ECG, revealed the epidemiological importance of CCHD in 1945. In 1953, the long period between initial infection and appearance of CCHD was established, whereas the annual incidence of CCHD from patients with the indeterminate form of the disease was established in 1956. The use of heart catheterization in 1965, exercise stress testing in 1973, Holter monitoring in 1975, Electrophysiologic testing in 1973, echocardiography in 1975, endomyocardial biopsy in 1981, and Magnetic Resonance Imaging in 1995, added to the fundamental clinical aspects of CCHD as described by Carlos Chagas.

Nota sobre ácaros (Acari) atacando fruteira-do-conde (Rollinia sp.)

The flowers of this orchard tree presents six fleshy petals, fused two by two, and disposed propeller like. In the center is a cavity which harbors the cone shaped reproductive organs. An eriophyid mite, described by H. H. Keifer as Aculops flechtmann Keifer, 1972, causes considerable bronzing to the petals (Fig. 1-A). Large numbers of this mite attacking the basis of the reproductive organs causes them to dry and drop. On the young fruits, which are squamous, this mite causes some rusting and small necrotic areas (Fig. 1-B). As the fruit grows these small areas remain dark and barky. Very often the necrotic areas are invaded by the false spider mite Brevipalpus phoenicis (Geijskes, 1939), which enlarges the damage. The false spider mite also attacks leaves and green stems; the epidermis of the latter then assumes a barky appearance.

Studies on the mineral nutrition and fertilization of the passion fruit plant (Passiflora edulis f. flavicarpa): II - placement of fertilizer phosphorus

A localização do superfosfato (marcado com P32) no maracujá em produção foi estudada em condições de plantação comercial. Verificou-se que as aplicações em sulcos circulares ou faixas superficiais ao redor da planta tem eficiência equivalente sendo esses métodos três vezes superiores à localização do adubo em furos no solo. A pulverização foliar, por sua vez, mostrou-se 20 vezes mais eficiente que a aplicação no solo de acordo com os dois primeiros métodos.

Studies on the mineral nutrition and fertilization of the passion fruit plant (Passiflora edulis F. flavicarpa): II. placement of fertilizer phosphorus

1. Tagged superphosphate was applied to 2.5 year old passion fruit plants from a commercial plantation established in a sandy loam. 2. 100 grams of the fertilizer were distributed in the following ways: in a circular furrow 20 cm around the plant 40 cm from the stems; in a circular strip 10 cm wide, 40 cm from the stems; in six holes around the plants, 40 cm from the stems 20 cm deep, 2.5 cm in diameter. 3. 10 grams of the fertilizer in 11 of water were sprayed to the leaves. 4. Three weeks after the treatments were made, leaf samples were taken for analysis. 5. Determinations of specific activities both in the leaves and in the fertilizer used have shown that R in the plant was derived from the superphosphate in the following relative proportions (by making the first treatment equal to 100): circular furrow = 100; circular strip = 120; holes = 30; foliar spray = 230.

The effect of chlorophenoxy propionamide (Fruitone CPA) on the fruit of banana cv. 'nanicão'

Fruitone CPA was applied on the two terminals hands of the banana bunch, using the following doses: 0-159-300 and 600 ppm. Fruitone did not affect fruit weightm but increased fruit lenght. This alteration was greater with Fruitone at 600 ppm.

The bacula of some fruit bats (Pteropus).

v.31:no.16(1947)

A new fruit pigeon from Nepal / Austin L. Rand and Robert L. Fleming.

v.34:no.19(1953)

Impact of genetic SLC28 transporter and ITPA variants on ribavirin 21 serum level, hemoglobin drop and therapeutic response in patients with HCV infection

Background: T reatment o f chronic hepatitis C i s evolving, a nd direct acting antivirals ( DAAs) are now a dded to p egylated interferon-α ( Peg- INF-α) and ribavirin (RBV) for the treatment o f hepatitis C v irus ( HCV) genotype 1 infection. DAAs c ause d ifferent side effects and can even worsen RBV induced hemolytic anemia. T herefore, identifying host genetic d eterminants of R BV bioavailability and therapeutic e fficacy will remain crucial for individualized treatment. Recent d ata showed associations between R BV induced h emolytic anemia and genetic polymorphisms o f concentrative nucleoside transporters s uch as C NT3 (SLC28A3) and i nosine t riphosphatase (ITPA). T o analyze t he association of genetic variants of SLC28 transporters and ITPA with RBV induced hemolytic anemia and treatment o utcome. Methods: I n our study, 173 patients f rom t he S wiss Hepatitis C C ohort Study and 2 2 patients from Swiss Association for the Study of the Liver study 24 (61% HCV g enotype 1, 3 9% genotypes 2 o r 3) were analyzed for SLC28A2 single nucleotide p olymorphism (SNP) rs11854484, SLC28A3 rs56350726 and SLC28A3 rs10868138 as well as ITPA SNPs rs1127354 and rs7270101. RBV serum levels during treatment were measured in 49 patients. Results: SLC28A2 r s11854484 genotype TT was associated with significantly higher dosage- and body weight-adjusted RBV levels as compared to genotypes TC and CC (p=0.04 and p=0.02 at weeks 4 and 8, respectively). ITPA SNPs rs1127354 and rs7270101 were associated with h emolytic a nemia both in genotype as w ell as i n allelic a nalyses. SLC28A3 rs56350726 genotype TT (vs. AT/AA, RR=2.1; 95% CI 1.1-4.1) as well as the T allele (vs. A; RR=1.8, 95% CI 1.1-3.2) were associated with increased SVR rates. The combined analysis of overall ITPA activity and SLC28 v ariants together revealed n o significant a dditive effects on either treatment-related anemia or SVR. Conclusions: T he newly identified association between RBV serum levels a nd SLC28A2 rs11854484 genotype as well as the replicated association of ITPA and SLC28A3 g enetic p olymorphisms w ith RBV induced hemolytic anemia and treatment r esponse underpin the need for further studies on host genetic d eterminants of R BV bioavailability and therapeutic e fficacy f or individualized treatment of chronic hepatitis C.

Shots de fruits et légumes : avantages et inconvénients [Fruit and vegetable beverages: advantages and disadvantages]

En Suisse, les recommandations de fruits et légumes sont de 600 g par jour. En réalité, la consommation moyenne se situe à 60% des recommandations, c'est-à-dire à 380 g par jour. L'utilisation de produits tels que des shots de fruits et légumes pourrait réduire l'écart en facilitant la consommation de ces aliments. Cependant, ils ne peuvent pas remplacer à cent pour cent les aliments frais car leurs teneurs en fibres et vitamines C sont plus faibles, et ils interfèrent avec la physiologie de la prise alimentaire (satiété). Ces produits peuvent donc faire partie d'une alimentation variée en prenant la place d'une portion de fruits et légumes. Un autre aspect non négligeable en est le coût puisque si l'on consommait ces produits sur une semaine à la place d'aliments frais la différence serait d'environ CHF 30.- de plus. In Switzerland, fruits and vegetables recommendations are of 600 gr per day. In reality, the average intake is about 60% of these recommendations, it means 380 gr per day. The use of products like fruits and vegetables shots could reduce the gap by making drinking easier. However, they cannot replace one hundred percent fresh food for their content in fibers and vitamin C are lower and they interfere with the physiology of the food intake (satiety). These products can have their importance in a various supply by taking the place of one fruit or vegetable portion. Another non negligible aspect is the price as if we consume these products over one week instead of fresh food, the difference would be of about CHF 30.- more

Trypanosomatid protozoa in fruit of solanaceae in southeastern Brazil

Fruits of cultivated and indigenous Solanaceae from Southeastern Brazil have been examined for the presence of trypanosomatid flagellates. The 14 species found infected were: Capsicum annum, C. praetermissum, Lycopersicon esculentum, Nicandra physaloides, Physalis angulata, Solanum sp., S. americanum, S. concinnum, S. diflorum, S. erianthum, S. gilo, S. robustum, S. variable and S. viarum. The pentatomid hemipteran Arvelius albopunctatus experimentally transmitted flagellates to fruits of some species. Cultures of flagellates were obtained form fruits of eight species of Salonaceae and from A. albopunctatus.