The dynamics of RNA participation in the vitellogenesis of Rhipicephalus sanguineus ticks Latreille 1806 (Acari: Ixodidae). I. Nucleoli or Cajal bodies?

To understand the morphological and histological aspects of internal systems of ticks has become important matter since these arthropods have an impact in the areas of the economy and public health. In this context, this study has provided morphological data on female germinative cells of Rhipicephalus sanguineus ticks, ectoparasites of dogs that maintain a close relationship with human on a daily basis. Oocytes of engorged females were analyzed, through the PAS reaction (detection of polysaccharides) counterstained by methyl green (detection of RNA) revealing information that allowed to infer for the first time the presence of Cajal bodies, in the germinal vesicles (nuclei) of developing oocytes, as well as showing how the RNA and the polysaccharides are involved in the dynamics of the vitellogenesis in this species. [copyright] 2010 Elsevier Ltd. All rights reserved.

High expectations, varying outcomes: Decentralization and participation in Brazil, Japan, Russia and Sweden

In the past 20 years, decentralization has been proposed as a strategy for enhancing public participation. Aid-providing organizations, such as the World Bank, stimulated decentralization processes in several countries in the hope that this would promote civic empowerment, diminish corruption, enhance efficiency, and improve public service delivery. This assumption forms the basis for a comparative analysis into the relation between decentralization and participation at the local level in Brazil, Japan, Russia and Sweden. A multi-level regression analysis using the data of the Democracy and Local Governance Project was undertaken in order to test the 'one size fits all' and the 'diversity in development' hypotheses. The results show that the second hypothesis was corroborated. Perceived autonomy had a different impact on openness to participation depending on the country considered; in one country (Japan), perceived autonomy diminished public officials' willingness to be open to public participation.

Politics, press and military participation in the abdication of d. Pedro I

This work seeks to contribute to a better understanding of the Abdication, analyzing how and why sections of the army joined the liberal groups against the emperor, focusing on the period that immediately preceded this event. The argument is that the alliance between sections of the army and the liberal groups in 1831 was possible because the expansion of the "public space" in the city of Rio de Janeiro, a process in which newspapers such as "O Republico" played a key role as they became a privileged locus for political disputes. The article shows that that newspaper helped to build a political identity based on the defense of Brazilian interests against Portuguese despotism, giving momentum to internal conflicts around this subject that were already taking place among sections of the army and hence triggering the process that would lead to the Abdication.

The Mexican film industry and its participation in the global value chain

Includes bibliography

Private participation in the provision of water services: alternative means for private participation in the provision of water services

Includes bibliography

Participation in the context of the world economy

Includes bibliography

Worker participation in company profits or operating results in Latin America

Includes bibliography

Descentralization and participation in Latin America: an economic perspective

Includes bibliography

The natural absence of RPA1N domain did not impair Leishmania amazonensis RPA-1 participation in DNA damage response and telomere protection.

We have previously shown that the subunit 1 of Leishmania amazonensis RPA (LaRPA-1) alone binds the G-rich telomeric strand and is structurally different from other RPA-1. It is analogous to telomere end-binding proteins described in model eukaryotes whose homologues were not identified in the protozoan's genome. Here we show that LaRPA-1 is involved with damage response and telomere protection although it lacks the RPA1N domain involved with the binding with multiple checkpoint proteins. We induced DNA double-strand breaks (DSBs) in Leishmania using phleomycin. Damage was confirmed by TUNEL-positive nuclei and triggered a G1/S cell cycle arrest that was accompanied by nuclear accumulation of LaRPA-1 and RAD51 in the S phase of hydroxyurea-synchronized parasites. DSBs also increased the levels of RAD51 in non-synchronized parasites and of LaRPA-1 and RAD51 in the S phase of synchronized cells. More LaRPA-1 appeared immunoprecipitating telomeres in vivo and associated in a complex containing RAD51, although this interaction needs more investigation. RAD51 apparently co-localized with few telomeric clusters but it did not immunoprecipitate telomeric DNA. These findings suggest that LaRPA-1 and RAD51 work together in response to DNA DSBs and at telomeres, upon damage, LaRPA-1 works probably to prevent loss of single-stranded DNA and to assume a capping function.

Acceso al poder y participación en la toma de decisiones en América Latina y el Caribe: políticas de equidad de género hacia el año 2000 = Access to power and participation in decision-making in Latin America and the Caribbean: policies for gender equity looking to the year 2000

Incluye Bibliografía

Alternatives for private participation in the provision of water services

Includes bibliography