Formas poco estudiadas del antiguo cancionero popular

Las composiciones formadas por tres o más pareados de métrica irregular han sido poco investigadas. Predominan las cancioncitas con dos versos; la forma más frecuente en que se desarrollaron durante los siglos xv y xvi fue el villancico. Las composiciones pareadas ya existían dentro de la región castellana desde el siglo xiii, y aunque durante los siglos posteriores desaparecieron de la superficie, las investigaciones folklorísticas modernas los han hecho emerger. Dentro de este tipo de "género" -si así podemos llamarlo-, son interesantes las composiciones no paralelísticas, que hablan de la cultura de campesinos y pastores. Este tipo de textos no se encuentra más que en los refraneros de la época. El encanto de las composiciones radica en la acumulación de sus elementos, como ocurre en las adivinanzas y en las rimas infantiles

Arquetipos populares, arquetipos cultos y sagrada escritura en la poesía popular española

En las coplas reunidas por Margit Frenk bajo el epígrafe: "Que si soy morena", así como en varias referidas a fiestas de la Virgen o agrupadas en otros temas, de índole amorosa, se cruzan varias tradiciones: el tema paneuropeo evocado por la misma investigadora de la afirmación del color moreno, la tradición asociada al nivel culto o noble de la belleza clara, incluso expresamente rubia y de ojos verdes estudiada desde Faral, Lecoy, Dámaso Alonso o María Rosa Lida y en fin una clara presencia bíblica, sobre todo del Cantar de los Cantares. Dada la intensa difusión y comentarios de este libro bíblico en los monasterios, especialmente benedictinos y cistercienses, y en la liturgia mariana, son muchas las vías posibles de interpenetración con la cultura popular.

Formas poco estudiadas del antiguo cancionero popular

Las composiciones formadas por tres o más pareados de métrica irregular han sido poco investigadas. Predominan las cancioncitas con dos versos; la forma más frecuente en que se desarrollaron durante los siglos xv y xvi fue el villancico. Las composiciones pareadas ya existían dentro de la región castellana desde el siglo xiii, y aunque durante los siglos posteriores desaparecieron de la superficie, las investigaciones folklorísticas modernas los han hecho emerger. Dentro de este tipo de "género" -si así podemos llamarlo-, son interesantes las composiciones no paralelísticas, que hablan de la cultura de campesinos y pastores. Este tipo de textos no se encuentra más que en los refraneros de la época. El encanto de las composiciones radica en la acumulación de sus elementos, como ocurre en las adivinanzas y en las rimas infantiles

Arquetipos populares, arquetipos cultos y sagrada escritura en la poesía popular española

En las coplas reunidas por Margit Frenk bajo el epígrafe: "Que si soy morena", así como en varias referidas a fiestas de la Virgen o agrupadas en otros temas, de índole amorosa, se cruzan varias tradiciones: el tema paneuropeo evocado por la misma investigadora de la afirmación del color moreno, la tradición asociada al nivel culto o noble de la belleza clara, incluso expresamente rubia y de ojos verdes estudiada desde Faral, Lecoy, Dámaso Alonso o María Rosa Lida y en fin una clara presencia bíblica, sobre todo del Cantar de los Cantares. Dada la intensa difusión y comentarios de este libro bíblico en los monasterios, especialmente benedictinos y cistercienses, y en la liturgia mariana, son muchas las vías posibles de interpenetración con la cultura popular.

Formas poco estudiadas del antiguo cancionero popular

Las composiciones formadas por tres o más pareados de métrica irregular han sido poco investigadas. Predominan las cancioncitas con dos versos; la forma más frecuente en que se desarrollaron durante los siglos xv y xvi fue el villancico. Las composiciones pareadas ya existían dentro de la región castellana desde el siglo xiii, y aunque durante los siglos posteriores desaparecieron de la superficie, las investigaciones folklorísticas modernas los han hecho emerger. Dentro de este tipo de "género" -si así podemos llamarlo-, son interesantes las composiciones no paralelísticas, que hablan de la cultura de campesinos y pastores. Este tipo de textos no se encuentra más que en los refraneros de la época. El encanto de las composiciones radica en la acumulación de sus elementos, como ocurre en las adivinanzas y en las rimas infantiles

Arquetipos populares, arquetipos cultos y sagrada escritura en la poesía popular española

En las coplas reunidas por Margit Frenk bajo el epígrafe: "Que si soy morena", así como en varias referidas a fiestas de la Virgen o agrupadas en otros temas, de índole amorosa, se cruzan varias tradiciones: el tema paneuropeo evocado por la misma investigadora de la afirmación del color moreno, la tradición asociada al nivel culto o noble de la belleza clara, incluso expresamente rubia y de ojos verdes estudiada desde Faral, Lecoy, Dámaso Alonso o María Rosa Lida y en fin una clara presencia bíblica, sobre todo del Cantar de los Cantares. Dada la intensa difusión y comentarios de este libro bíblico en los monasterios, especialmente benedictinos y cistercienses, y en la liturgia mariana, son muchas las vías posibles de interpenetración con la cultura popular.

Search for the pathogenesis of the differing phenotype in two compound heterozygote Hungarian brothers with the same genotypic triosephosphate isomerase deficiency

In a Hungarian family with triosephosphate isomerase (TPI) deficiency, two compound heterozygote brothers were found with the same severe decrease in TPI activity, but only one of them had the classical symptoms. In search for the pathogenesis of the differing phenotype of the same genotypic TPI deficiency, an increase in red cell membrane fluidity was found. There were roughly 100% and 30% more 16:0/20:4 and 18:0/20:4 diacyl-phosphatidylcholine species in erythrocytes from the two TPI-deficient brothers than in the probes from healthy controls. The activities of acethylcholinesterase and calmodulin induced Ca2+ ATPase were significantly enhanced in erythrocytes from the propositus as compared with those of the neurologically symptom-free brother and other members of the TPI-deficient family as well as to those from healthy controls. Both enzymes are crucially involved in the function of nerve cells. The observed differences in membrane fluidity and enzyme activities between the erythrocytes from the phenotypically differing TPI-deficient brothers underline the importance of investigations into the effect of biophysical changes in the lipid environment of the membrane proteins on the development of disseminated focal neurological disorders of unknown pathogenic origin.

Shifts of Intracellular pH Distribution as a Part of the Signal Mechanism Leading to the Elicitation of Benzophenanthridine Alkaloids1 : Phytoalexin Biosynthesis in Cultured Cells of Eschscholtzia californica

Cultured cells of Eschscholtzia californica (Californian poppy) respond to a yeast elicitor preparation or Penicillium cyclopium spores with the production of benzophenanthridine alkaloids, which are potent phytoalexins. Confocal pH mapping with the probe carboxy-seminaphthorhodafluor-1-acetoxymethylester revealed characteristic shifts of the pH distribution in challenged cells: within a few minutes after elicitor contact a transient acidification of cytoplasmic and nuclear areas occurred in parallel with an increase of the vacuolar pH. The change of proton concentration in the vacuole and in the extravacuolar area showed a nearly constant relation, indicating an efflux of vacuolar protons into the cytosol. A 10-min treatment with 2 mm butyric or pivalic acid caused a transient acidification of the cytoplasm comparable to that observed after elicitor contact and also induced alkaloid biosynthesis. Experimental depletion of the vacuolar proton pool reversibly prevented both the elicitor-triggered pH shifts and the induction of alkaloid biosynthesis. pH shifts and induction of alkaloid biosynthesis showed a similar dependence on the elicitor concentration. Net efflux of K+, alkalinization of the outer medium, and browning of the cells were evoked only at higher elicitor concentrations. We suggest that transient acidification of the cytoplasm via efflux of vacuolar protons is both a necessary and sufficient step in the signal path toward biosynthesis of benzophenanthridine alkaloids in Californian poppy cells.

The human formin-binding protein 17 (FBP17) interacts with sorting nexin, SNX2, and is an MLL-fusion partner in acute myelogeneous leukemia

We have cloned a fusion partner of the MLL gene at 11q23 and identified it as the gene encoding the human formin-binding protein 17, FBP17. It maps to chromosome 9q34 centromeric to ABL. The gene fusion results from a complex chromosome rearrangement that was resolved by fluorescence in situ hybridization with various probes on chromosomes 9 and 11 as an ins(11;9)(q23;q34)inv(11)(q13q23). The rearrangement resulted in a 5′-MLL/FBP17-3′ fusion mRNA. We retrovirally transduced murine-myeloid progenitor cells with MLL/FBP17 to test its transforming ability. In contrast to MLL/ENL, MLL/ELL and other MLL-fusion genes, MLL/FBP17 did not give a positive readout in a serial replating assay. Therefore, we assume that additional cooperating genetic abnormalities might be needed to establish a full malignant phenotype. FBP17 consists of a C-terminal Src homology 3 domain and an N-terminal region that is homologous to the cell division cycle protein, cdc15, a regulator of the actin cytoskeleton in Schizosaccharomyces pombe. Both domains are separated by a consensus Rho-binding motif that has been identified in different Rho-interaction partners such as Rhotekin and Rhophilin. We evaluated whether FBP17 and members of the Rho family interact in vivo with a yeast two-hybrid assay. None of the various Rho proteins tested, however, interacted with FBP17. We screened a human kidney library and identified a sorting nexin, SNX2, as a protein interaction partner of FBP17. These data provide a link between the epidermal growth factor receptor pathway and an MLL fusion protein.

Historic records of organic compounds from a high Alpine glacier: influences of biomass burning, anthropogenic emissions, and dust transport

Historic records of α-dicarbonyls (glyoxal, methylglyoxal), carboxylic acids (C6–C12 dicarboxylic acids, pinic acid, p-hydroxybenzoic acid, phthalic acid, 4-methylphthalic acid), and ions (oxalate, formate, calcium) were determined with annual resolution in an ice core from Grenzgletscher in the southern Swiss Alps, covering the time period from 1942 to 1993. Chemical analysis of the organic compounds was conducted using ultra-high-performance liquid chromatography (UHPLC) coupled to electrospray ionization high-resolution mass spectrometry (ESI-HRMS) for dicarbonyls and long-chain carboxylic acids and ion chromatography for short-chain carboxylates. Long-term records of the carboxylic acids and dicarbonyls, as well as their source apportionment, are reported for western Europe. This is the first study comprising long-term trends of dicarbonyls and long-chain dicarboxylic acids (C6–C12) in Alpine precipitation. Source assignment of the organic species present in the ice core was performed using principal component analysis. Our results suggest biomass burning, anthropogenic emissions, and transport of mineral dust to be the main parameters influencing the concentration of organic compounds. Ice core records of several highly correlated compounds (e.g., p-hydroxybenzoic acid, pinic acid, pimelic, and suberic acids) can be related to the forest fire history in southern Switzerland. P-hydroxybenzoic acid was found to be the best organic fire tracer in the study area, revealing the highest correlation with the burned area from fires. Historical records of methylglyoxal, phthalic acid, and dicarboxylic acids adipic acid, sebacic acid, and dodecanedioic acid are comparable with that of anthropogenic emissions of volatile organic compounds (VOCs). The small organic acids, oxalic acid and formic acid, are both highly correlated with calcium, suggesting their records to be affected by changing mineral dust transport to the drilling site.

Magyarország /

Includes index.

Sancti Eusebii Hieronymi Epistulae /

Includes bibliographical references and indexes.

Cytotoxicity of topical antimicrobial agents used in burn wounds in Australasia

Background: Burn sepsis is a leading cause of mortality and morbidity in patients with major burns. The use of topical antimicrobial agents has helped improve the survival of these patients. Silvazine (Sigma Pharmaceuticals, Melbourne, Australia) (1% silver sulphadiazine and 0.2% chlorhexidine digluconate) is used exclusively in Australasia, and there is no published study on its cytotoxicity. This study compared the relative cytotoxicity of Silvazine with 1% silver sulphadiazine (Flamazine (Smith & Nephew Healthcare. Hull. UK)) and a silver-based dressing (Acticoat (Smith & Nephew Healthcare, Hull, UK)). Methods: Dressings were applied to the centre of culture plates that were then seeded with keratinocytes at an estimated 25% confluence. The plates were incubated for 72 h and culture medium and dressings then removed. Toluidine blue was added to stain the remaining keratinocytes. Following removal of the dye, the plates were photographed under standard conditions and these digital images were analysed using image analysis software. Data was analysed using Student's t-test. Results: In the present study, Silvazine is the most cytotoxic agent. Seventy-two hour exposure to Silvazine in the present study results in almost no keratinocyte survival at all and a highly statistically significant reduction in cell survival relative to control, Acticoat and Flamazine (P