Crescimento, características nutricionais e fisiológicas de progênies de Eucalyptus grandis W. Hill. Ex Maiden sob diferentes doses de fósforo

Pós-graduação em Agronomia (Produção Vegetal) - FCAV

Selecting for rust (Puccinia psidii) reistance in Euclyptus grandis in São Paulo state, Brazil

In Brazil, Eucalyptus grandis is a key species for wood production. However, some genotypes are susceptible to rust (Puccinia psidii), mainly in São Paulo State, where climatic conditions are favorable for its development. Rust represents a high economic risk to forest companies because of the high potential of damage to commercial eucalypt plantations. The aims of the present study were (i) to select progenies of E. grandis for stability and adaptability regarding resistance to rust at different locations; (ii) compare the selections under these different climatic conditions; and (iii) compare rust severity in the field with the theoretical model. We observed that climatic conditions were extremely influential factors for rust development, but even under favorable conditions for disease development, we found rust-resistant progenies. In sites unfavorable for rust development, we detected highly susceptible progenies. We found significant correlation among the genetic material, environmental conditions and disease symptoms, however, we observed a simple genotype-environmental interaction and significant genetic variability among the progenies. The average heritability was high among the progenies in all sites, indicating substantial genetic control for rust resistance. We also observed a good relationship between rust severity in the field and the theoretical model that considered annual average temperature and leaf wetness.

Can hydrogen peroxide and quercetin improve production of Eucalyptus grandis x Eucalyptus urophylla?

Vegetative propagation is considered the best choice for the rapid multiplication of plant species, however, rooting may still present difficulties. Substances, such as auxins, phenolic compounds and hydrogen peroxide, are recognized as able to improve this process. The aim of the present work was to determine if hydrogen peroxide in combination with quercetin or indole butyric acid, can modify some characteristics related to rooting and development in cuttings of Eucalyptus grandis x Eucalyptus urophylla. Cuttings were periodically evaluated at 30, 60 and 90 days according to the following criteria: height, diameter and survival percentage. After planting (90 days), a destructive evaluation was performed to determine rooting percentage, average size and number of roots. Polyamines content and polyamine oxidase activity, as biochemical markers of plant development, were determined. No statistically significant differences in height, diameter, survival and rooting percentage, root length and number of roots per cuttings were found. Treatments induced a decrease in putrescine levels and polyamine oxidase activity in roots. For absence of positive responses, the use of these substances as a treatment to improve cutting production is economically unviable.

Variação dimensional de tábuas de Eucalyptus grandis durante alguns níveis de tempo e temperatura de termorretificação

The objective of this study was evaluate the effects of retification® temperatures and times on thickness variation from Eucalyptus grandis timber. Boards from logs of 30-year-old Eucalyptus grandis trees, from São Paulo Forest Institute in Santa Barbara, Brazil, were thermally modified at 180ºC for 2.5 hours, at 200ºC for 3.0 hours and 200ºC for 4.0 hours. The results showed that: (1) the thermally modified wood at 200°C by 3.0 to 4.0 hours showed increased thickness between 0.698 mm and 0.874 mm due to the internal cracks, (2) the thermally modified wood at 180°C by 2.5 hours has a decreased from 0,177 mm in thickness and the absences of internal cracks.

Modelo matemático aplicado na vaporização de toras de Eucalyptus grandis

O objetivo deste estudo é obter um modelo matemático logístico que representa a vaporização de toras de Eucalyptus grandis, em função do diâmetro e do tempo de permanência das toras no tratamento térmico a vapor. Para tanto, foram coletadas toras nas classes diamétricas de 25 a 40 cm de três árvores de E. grandis provenientes do Horto Florestal de Mandurí, SP. Em cada uma das toras foram inseridos três termopares, com profundidades distintas, 1/3 do raio; 2/3 do raio e o terceiro, próximo ao seu centro. Posteriormente, as toras foram vaporizadas a 85ºC e 100% de umidade relativa, até que o último termopar atingisse uma temperatura de 80ºC. Um Datalogger CR10 registrou as temperaturas, no interior do material, durante o tratamento térmico. Conclui-se que o modelo matemático logístico foi bem ajustado, obtido no tratamento térmico com vapor, levando em consideração as três classes de diâmetro e a profundidade máxima de 50 mm de raio, o modelo gerado, apresenta-se uma viabilidade técnica na aplicação do processo de aquecimento de toras com vapor, promovendo um consumo ideal de energia.

Variabilidade genética e alterações morfológicas em progênies de polinização aberta de Eucalyptus grandis sob o efeito do paclobutrazol

The research objective was to study the genetic diversity of morphological traits in Eucalyptus grandis progenies growing under paclobutrazol regulator effects. The progeny trail was set up through design of randomized blocks. The morphological changes occurred before and during the plant flowering were analyzed. The estimation of genetic parameters were for plant height and stem diameter. The paclobutrazol have caused changes on plant development being strong by the beginning and becoming lightening through the evaluations. The coefficients of variation have shown there is higher genetic diversity within than among progenies for the studied traits. Therefore, it can have high efficiency on selection within progenies in the Eucalyptus grandis breeding program.

Aplicação de polímero hidroretentor no desenvolvimento inicial de Eucalyptus grandis

Pós-graduação em Agronomia (Proteção de Plantas) - FCA

Organization of Sporulated Oocysts of Eimeria funduli in the Gulf Killifish, Fundulus grandis

Oocysts of Eimeria funduli were studied by transmission electron microscopy in naturally-infected livers of the Gulf killifish, Fundulus grandis. Tissues were cryo-processed because membranous structures in the oocyst appear to hinder routine fixation and embedment. The oocyst wall (about 25 nm thick) was adjacent to the host cell and consisted of an outer membrane that limited the host cell cytoplasm and an inner membrane separated from the outer membrane by a narrow space. In some specimens, dense material was applied to the inner face of the inner membrane. Individual sporocysts were surrounded by a membranous "veil" (about 25 nm thick) that consisted of two unit membranes. Sporopodia, projections of the sporocyst wall, supported the veil. The sporocyst wall (130-150 nm thick) consisted of two layers, a thin electron-lucent outer layer (about 10 nm thick) and a thick electron dense inner layer (about 130 nm thick). Depending on the plane of section, the inner layer had transverse striations with periods of 3 to 4 nm or 12 to 15 nm. A narrow fissure, broadest at the anterior pole of the sporocyst, extended about one-third the length of the sporocyst wall. The posterior pole of the sporocyst was characterized by a bulbous swelling. Although this swelling resembled a Stieda body in light microscopic preparations, ultrastructurally, the swelling was a knoblike thickening in the sporocyst wall and did not plug a gap in this wall

Aplicação de polímero hidroretentor no desenvolvimento inicial de Eucalyptus grandis

Pós-graduação em Agronomia (Proteção de Plantas) - FCA

EFFECT OF THERMAL TREATMENT ON THE CHEMICAL CHARACTERISTICS OF WOOD FROM Eucalyptus grandis W. Hill ex Maiden UNDER DIFFERENT ATMOSPHERIC CONDITIONS

Thermal treatment (thermal rectification) is a process in which technological properties of wood are modified using thermal energy, the result of Which is often value-added wood. Thermally treated wood takes on similar color shades to tropical woods and offers considerable resistance to destructive microorganisms and climate action, in addition to having high dimensional stability and low hygroscopicity. Wood samples of Eucalyptus grandis were subjected to various thermal treatments, as performed in presence (140 degrees C; 160 degrees C; 180 degrees C) or in absence of oxygen (160 degrees C; 180 degrees C; 200 degrees C) inside a thermal treatment chamber, and then studied as to their chemical characteristics. Increasing the maximum treatment temperatures led to a reduction in the holocellulose content of samples as a result of the degradation and volatilization of hemicelluloses, also leading to an increase in the relative lignin content. Except for glucose, all monosaccharide levels were found to decrease in samples after the thermal treatment at a maximum temperature of 200 degrees C. The thermal treatment above 160 degrees C led to increased levels of total extractives in the wood samples, probably ascribed to the emergence of low molecular weight substances as a result of thermal degradation. Overall, it was not possible to clearly determine the effect of presence or absence of oxygen in the air during thermal treatment on the chemical characteristics of the relevant wood samples.

Production and carbon allocation in monocultures and mixed-species plantations of Eucalyptus grandis and Acacia mangium in Brazil

Introducing nitrogen-fixing tree species in fast-growing eucalypt plantations has the potential to improve soil nitrogen availability compared with eucalypt monocultures. Whether or not the changes in soil nutrient status and stand structure will lead to mixtures that out-yield monocultures depends on the balance between positive interactions and the negative effects of interspecific competition, and on their effect on carbon (C) uptake and partitioning. We used a C budget approach to quantify growth, C uptake and C partitioning in monocultures of Eucalyptus grandis (W. Hill ex Maiden) and Acacia mangium (Willd.) (treatments E100 and A100, respectively), and in a mixture at the same stocking density with the two species at a proportion of 1 : 1 (treatment MS). Allometric relationships established over the whole rotation, and measurements of soil CO2 efflux and aboveground litterfall for ages 4-6 years after planting were used to estimate aboveground net primary production (ANPP), total belowground carbon flux (TBCF) and gross primary production (GPP). We tested the hypotheses that (i) species differences for wood production between E. grandis and A. mangium monocultures were partly explained by different C partitioning strategies, and (ii) the observed lower wood production in the mixture compared with eucalypt monoculture was mostly explained by a lower partitioning aboveground. At the end of the rotation, total aboveground biomass was lowest in A100 (10.5 kg DM m(-2)), intermediate in MS (12.2 kg DM m(-2)) and highest in E100 (13.9 kg DM m(-2)). The results did not support our first hypothesis of contrasting C partitioning strategies between E. grandis and A. mangium monocultures: the 21% lower growth (delta B-w) in A100 compared with E100 was almost entirely explained by a 23% lower GPP, with little or no species difference in ratios such as TBCF/GPP, ANPP/TBCF, delta B-w/ANPP and delta B-w/GPP. In contrast, the 28% lower delta B-w in MS than in E100 was explained both by a 15% lower GPP and by a 15% lower fraction of GPP allocated to wood growth, thus partially supporting our second hypothesis: mixing the two species led to shifts in C allocations from above- to belowground, and from growth to litter production, for both species.

SEASONAL INCREMENT IN TRUNK DIAMETER OF Eucalyptus grandis TREES APPLYING DENDROMETER BANDS

The present work aimed to evaluate the seasonal increment in diameter of Eucalyptus grandis trees for 24 months and its relationship with the climatic variables and fertilization with nitrogen and with sewer mud. The trees were planted in the spacing of 3 x 2 m and fertilized with nitrogen (planting, 6, 12, 18 months) and sewer mud (planting and 8 months). 20 trees were selected by treatment according witch the distribution of basal area and installed dendrometer bands at a 1.3 meter. The results showed a clear effect of the climatic variables on the seasonal increment in diameter of trees, being observed a delay period (lag) of 28 days for the answer of the trees in relation to the climatic variables. Regading to the fertilization effect, it was observed that the increment of trunk diameter was higher in the eucalypt trees with organic in relation to mineral fertilization with nitrogen.

X-ray Microdensitometry Applied to Determination of Wood Density Variation of Eucalyptus grandis W. Hill trees

This study aimed to analyze the variation of wood density in the radial and longitudinal trunk of Eucalyptus grandis trees. Six 23 years old trees were selected and cross sections were cut in three longitudinal positions (DBH, 3.70, 6.10 m) of the log. The results showed that the apparent density of wood (i) increases in the radial direction, characterizing the juvenile wood and mature wood, (ii) no significant variation in base-top log direction was observed. Based on the radial profiles of density, the mature wood of Eucalyptus grandis can be applied in the manufacture of products with higher aggregated value (PMVAs).

Stand-level patterns of carbon fluxes and partitioning in a Eucalyptus grandis plantation across a gradient of productivity, in Sao Paulo State, Brazil

Wood production represents a large but variable fraction of gross primary production (GPP) in highly productive Eucalyptus plantations. Assessing patterns of carbon (C) partitioning (C flux as a fraction of GPP) between above- and belowground components is essential to understand mechanisms driving the C budget of these plantations. Better knowledge of fluxes and partitioning to woody and non-woody tissues in response to site characteristics and resource availability could provide opportunities to increase forest productivity. Our study aimed at investigating how C allocation varied within one apparently homogeneous 90 ha stand of Eucalyptus grandis (W. Hill ex Maiden) in Southeastern Brazil. We assessed annual above-ground net primary production (ANPP: stem, leaf, and branch production) and total belowground C flux (TBCF: the sum of root production and respiration and mycorrhizal production and respiration), GPP (computed as the sum of ANPP, TBCF and estimated aboveground respiration) on 12 plots representing the gradient of productivity found within the stand. The spatial heterogeneity of topography and associated soil attributes across the stand likely explained this fertility gradient. Component fluxes of GPP and C partitioning were found to vary among plots. Stem NPP ranged from 554 g C m(-2) year(-1) on the plot with lowest GPP to 923 g C m(-2) year(-1) on the plot with highest GPP. Total belowground carbon flux ranged from 497 to 1235 g C m(-2) year(-1) and showed no relationship with ANPP or GPP. Carbon partitioning to stem NPP increased from 0.19 to 0.23, showing a positive trend of increase with GPP (R-2 = 0.29, P = 0.07). Variations in stem wood production across the gradient of productivity observed at our experimental site were a result of the variability in C partitioning to different forest system components.

EFEITO DA IDADE E POSIÇÃO DE AMOSTRAGEM NA DENSIDADE E CARACTERÍSTICAS ANATÔMICAS DA MADEIRA DE Eucalyptus grandis

The knowledge of the variations in the wood characteristics produced by eucalyptus trees according to age and sampling positions is essential for its proper use. This study had as objective to evaluate the influence of the age, longitudinal and radial positions on basic density and anatomical characteristics in Eucalyptus grandis wood. The trees were planted in 3x2 m spacing and fertilized with commercial fertilizers in planting, 6th and 12th months. According to basal area distribution, fifteen trees were selected (24, 36 and 72 months of age) - five trees per age. Disks at DBH position (1.3 m) were taken for fiber determination (length, wall thickness, lumen diameter and width) and vessels (tangential diameter, frequency and area occupied) and in other different sampling positions for basic density determination. Wood basic density increased from 0.43 to 0.46 g.cm(-3) as well as the trees age increases with a longitudinal variation model, characterized through a decrease in base-3m (0.42-0.49 -> 0.40-0.46 g.cm(-3)) and an increase to the top of the trunk (0.46 -> 0.54 g.cm(-3)) Fibers and vessels dimensions showed variations related to age and to pit-bark direction. Wood properties behavior and variations indicate that, until this period, the juvenile wood is being formed.