Localization of Antigenic Sites in Unfed Nymphs of Amblyomma triste Koch 1844 (Acari: Ixodidae) Ticks by Immunohistochemistry

P>The reactivity of sera collected from guinea pigs after three infestations with Amblyomma triste nymphs on histological sections of the same tick species was investigated through immunohistochemistry to identify potential target cells and tissues. Six guinea pigs were infested thrice, at 30 day intervals, with 30 nymphs of A. triste per animal per infestation. Blood samples were collected from the guinea pigs 15 days after each infestation for serum separation; normal serum was obtained before the first infestation as control. Unfed A. triste nymphs' histological sections were submitted to indirect immunohistochemistry technique by using normal or hyperimmune guinea pig serum as primary antibody and a goat IgG-alkaline phosphatase-APase conjugate as secondary antibody. A weak to moderate APase activity was observed in cells of salivary glands, midgut and haemolymph of unfed nymphs incubated with hyperimmune serum.

Dinâmica da vitelogêneses de carrapatos Amblyomma triste (Koch, 1844) (Acari: Ixodidae)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

A fuga dupla luso-brasileira durante os séculos XVIII e XIX

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação da resistência em caprinos a ninfas do carrapato Amblyomma cajennense (Fabricius, 1787) e da reatividade cruzada com A. hebraeum (Koch, 1844) (Acari:Ixodidae)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Diversidade de helmintos de Notothenia coriiceps Richardson, 1844 da Baía do Almirantado, Ilha do Rei George, Antártica

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Biologia reprodutiva de toninha, Pontoporia blainvillei (Gervais & D'Orbigny, 1844) (Mammalia Cetacea), no litoral sul de São Paulo e norte do Paraná

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Hábitos alimentares de Toninha, Pontoporia blainvillei (Gervais e D'Orbigny, 1844) (Mammalia, Cetacea), no sul do Estado de São Paulo e norte do Paraná, Brasil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Heroísmos, sedições e heresias: a construção do ufanismo e do ressentimento nos sertões da capitania de São Paulo (1768-1774)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

A formação das famílias livres: Campinas, 1774-1850

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

A educação sexual no Brasil colônia prescrita nos Regimentos do Santo Ofício da Inquisição Portuguesa (1552-1774)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

How the fluctuations of water levels affect populations of invasive bivalve Corbicula fluminea (Muller, 1774) in a Neotropical reservoir?

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Gross and microscopic observations on the lingual structure of the franciscana (Pontoporia blainvillei-gervais and d'orbigny, 1844)

In most anatomical studies developed with mammals, the tongue is described as highly differentiated among different species. However, studies on the tongue of aquatic mammals are still limited as compared to those on terrestrial mammals. The aim of this study was to describe the tongue morphology of the Franciscana dolphin (Pontoporia blainvillei) using macroscopic observations, light, and scanning electron microscopy. Microscopically, the dorsal surface was covered by a keratinized stratified epithelium. Salivary gland acini were found on the middle and caudal third of the tongue. The dorsal surface was totally covered by filiform papillae with a connective tissue core and a connective tissue structure round in shape in the middle and caudal regions. Microsc. Res. Tech. 75:737742, 2012. (C) 2012 Wiley Periodicals, Inc.

Redescription of the larva of Amblyomma oblongoguttatum Koch, 1844 (Acari: Ixodidae) by light and scanning electron microscopy

The larval stage of Amblyomma oblongoguttatum Koch is redescribed using optical and scanning electron microscopy. Unfed larvae were obtained from a colony of A. oblongoguttatum originated from engorged females collected on domestic pigs from Monte Negro municipally (10 degrees 29'S, 63 degrees 32'W), State of Rondonia, Western Amazon, Brazil. Several characters are presented including the chaetotaxy of the idiosoma, palpi and Haller's organ, as well as morphological features of the idiosoma, gnathosoma and legs. In addition, the porotaxy (topographical and numerical patterns of integumentary structures) were presented by using a new nomenclature recently proposed. The chaetotaxy of the larvae of A. oblongoguttatum, in general, is similar to other Neotropical Amblyomma species. Three types of integumentary structures were observed on the idiosoma: lyrifissures, small glands, and large wax glands. Topographic and numerical patterns of the integumentary structures consisted of 5 pairs of large wax glands (1 dorsal/4 ventral), 24 pairs of lyrifissures (11 dorsal/13 ventral), and 49 pairs of small glands (28 dorsal/21 ventral). These topographic and numerical patterns found for A. oblongoguttatum show only minor differences when compared with patterns of other Amblyomma larvae, however, a few key features can be used for identification of these species.

Rickettsia bellii in ticks Amblyomma varium Koch, 1844, from birds in Peru

Amazonian birds were caught and examined for the presence of ectoparasites in the Allpahuayo Mishana National Reserve near Iquitos, Peru, from 13 to 16 August 2011. A total of 40 birds representing 16 species were examined. Two birds (5%) were infested with 2 larvae of Amblyomma varium Koch, 1844, and one nymph of A. calcaratum Neumann, 1899. The 2 larvae of A. varium were infected with Rickettsia bellii. This is the first report of R. bellii in A. varium and also the first record of this rickettsia in Peru. In addition, an immature A. calcaratum is reported from Peru for the first time. (c) 2012 Elsevier GmbH. All rights reserved.

A surrogate life cycle of Amblyomma ovale Koch, 1844

As ticks spend most of their time off-host, the environment is a major selective force of these parasites. In fact, human impact on landscapes has favored a minority of tick species which became well-known pests. However, this is an ongoing process and novel pests may arise. We herein report a surrogate life cycle of a neotropical tick species. Amblyomma ovate, and which may be related to an increased risk of human rickettsiosis. Under natural conditions, adults of this tick species feed on carnivores and exhibit non-nidicolous ambush behavior, whereas larvae and nymphs feed on small rodents and birds. In an anthropized spot within an Atlantic rainforest reserve of Brazil, an A. ovate population exhibited a nidicolous behavior with all 3 tick stages feeding on the dog. This dog's infestation was outstandingly high, and it displayed the highest anti-Rickettsia titers and harbored Rickettsia-infected ticks. (c) 2012 Elsevier GmbH. All rights reserved.