Ion-Ion correlations effect on nonlinear high-frequency plasma conductivity. AT(30-1)-1238-MATT-675

On the basis of the BBGKY hierarchy of equations an expression is derived for the response of a fully ionized plasma to a strong, high-frequency electric field in the limit of infinite ion mass. It is found that even in this limit the ionion correlation function is substantially affected by the field. The corrections to earlier nonlinear results for the current density appear to be quite ssential. The validity of the model introduced by Dawson and Oberman to study the response to a vanishingly small field is confirmed for larger values of the field when the eorrect expression for the ion-ion correlations i s introduced; the model by itself does not yield such an expression. The results have interest for the heating of the plasma and for the propagation of a strong electromagnetic wave through the plasma. The theory seems to be valid for any field intensity for which the plasma is stable.

Breve diccionario valenciano-castellano

Sign. : *8, 2*3, A-H8, I3

Conversación sobre el Diario de los Literatos de España

Plácido Veranio es seud. de G. Mayans

Noticia historica de los principios, progreso, y ereccion de la Real Academia de las nobles artes ... establecida en Valencia con el titulo de San Carlos, y relacion de los premios que distribuyo en la Junta Publica celebrada en 18 de Agosto de 1773

Sign. : [ ]1, A-H4, I3

Arithmetica demonstrada theorico-practica para lo mathematico y mercantil explicanse las monedas, pesos y medidas de los Hebreos, Griegos y Romanos, y de estos Reynos de España, conferidas entre sí

Índice

Ordenanzas del gremio de los fabricantes de jabon y almidon de... Valencia... aprobadas y mandadas observar por Real Provision de su Magestad de 4 de Deciembre de 1739 publicadas en... 1740

Sign. : []2, B-H2, I3

La pobreza del hospital de En-Bou ... por Don Manuel Rovira y Bou ... sobre no deber su tercio-diezmo contribuir a la gracia del Escusado

Precede al texto 1 h. con cita en latín

Oracion fúnebre, que en las ... exêquias celebradas por ... D. Carlos Joseph Gutierrez de los Rios, Fernandez de Córdoba ... sexto conde de Fernan-Nuñez ...

Marca tip. en port

Alegacion del derecho que assiste a Don Melchor de Calatayud... y a Sor Juana y Sor Leonor de Calatayud... en el pleyto contra Don Luis, Don Lorenzo de Calatayud y Doña Francisca Ayerve... sobre la succession de ciertos fideicomisos... [Texto impreso]

[]1, A-H2, I3

Oratio in Studiorum Instauratione [Texto impreso] : ad Senatum et Academiam Valentinam habita

Cebecera xil.: "Luis Domingo, in. ; Manuel Monfort ft."

Informacion en derecho sobre la institucion de la dignidad de Lugarteniente General... en la... Religion militar de N. Señora de Montesa... [Texto impreso] : su precedencia a las demas dignidades de la Orden y preeminencias...

Sign.: []1, A1, B-H2, I3

Capítols fets e fermats per los particulars del offici de torners de la present ciutat de Valencia [Manuscrito]

Recortado afectando a la foliación original

Reglas, y reflexiones cristianas [Texto impreso] : dirigidas a las Hermanas Hospitaleras, que en el año 1757 se congregaron en el Hospital General de la Ciudad de Valencia ...

Índice

Gi regulation of secretory vesicle swelling examined by atomic force microscopy

In the last decade, several monomeric and heterotrimeric guanine nucleotide binding proteins have been identified to associate with secretory vesicles and to be implicated in exocytosis. Vesicle volume also has been proposed to play a regulatory role in secretory vesicle fusion at the plasma membrane. However, the molecular mechanism of function of the guanine nucleotide binding proteins and of the regulation of secretory vesicle volume in the exocytotic process remains unclear. In this study, we report association of the secretory vesicle membrane with the α subunit of a heterotrimeric GTP binding protein Gαi3 and implicate its involvement in vesicle swelling. Using an atomic force microscope in combination with confocal microscopy, we were able to study the dynamics of isolated zymogen granules, the secretory vesicles in exocrine pancreas. Exposure of zymogen granules to GTP resulted in a 15–25% increase in vesicle height as measured by the atomic force microscope and a similar increase in vesicle diameter as determined by confocal microscopy. Mas7, an active mastoparan analog known to stimulate Gi proteins, was found to stimulate the GTPase activity of isolated zymogen granules and cause swelling. Increase in vesicle size in the presence of GTP, NaF, and Mas7 were irreversible and KCl-sensitive. Ca2+ had no effect on zymogen granule size. Taken together, the results indicate that Gαi3 protein localized in the secretory vesicle membrane mediates vesicle swelling, a potentially important prerequisite for vesicle fusion at the cell plasma membrane.

RGS-GAIP, a GTPase-activating Protein for Gαi Heterotrimeric G Proteins, Is Located on Clathrin-coated Vesicles

RGS-GAIP (Gα-interacting protein) is a member of the RGS (regulator of G protein signaling) family of proteins that functions to down-regulate Gαi/Gαq-linked signaling. GAIP is a GAP or guanosine triphosphatase-activating protein that was initially discovered by virtue of its ability to bind to the heterotrimeric G protein Gαi3, which is found on both the plasma membrane (PM) and Golgi membranes. Previously, we demonstrated that, in contrast to most other GAPs, GAIP is membrane anchored and palmitoylated. In this work we used cell fractionation and immunocytochemistry to determine with what particular membranes GAIP is associated. In pituitary cells we found that GAIP fractionated with intracellular membranes, not the PM; by immunogold labeling GAIP was found on clathrin-coated buds or vesicles (CCVs) in the Golgi region. In rat liver GAIP was concentrated in vesicular carrier fractions; it was not found in either Golgi- or PM-enriched fractions. By immunogold labeling it was detected on clathrin-coated pits or CCVs located near the sinusoidal PM. These results suggest that GAIP may be associated with both TGN-derived and PM-derived CCVs. GAIP represents the first GAP found on CCVs or any other intracellular membranes. The presence of GAIP on CCVs suggests a model whereby a GAP is separated in space from its target G protein with the two coming into contact at the time of vesicle fusion.