The control of indirect effects of biomanipulation

Results from long-term investigations on biomanipulation show that indirect effects are at least as important as direct effects are for the stability of biomanipulation. Three types of indirect effects can be distinguished: (1) a change in quantity or quality of the resource base, (2) behavioural change of the prey, and (3) development of anti-predator traits. Although indirect effects of type (2), (e.g. a change in the pattern of vertical migration of zooplankton), and type (3), (e.g. development of helmets and neck teeth in Daphnia), are important mechanisms, the most essential indirect effects regarding biomanipulation belong to type (1). An example of the latter will be demonstrated: the complex of indirect effects of enhanced grazing by large herbivores on the phosphorus metabolism of the lake. It is concluded that control of the indirect effects is absolutely necessary to stabilize biomanipulation measures, but this is much more difficult than the control of direct effects and needs deeper insights into the structuring mechanisms of food webs. Proper management of fish stocks, in combination with the control of phosphorus load and/or the physical conditions, seems to be the most promising way of controlling the indirect effects of biomanipulation.

Physical measures to inhibit planktonic cyanobacteriae

In a small lake, intermittent destratification was installed after several other physico-chemical and physical in-lake therapy measures (phosphorus immobilization, permanent destratification) had been tested without great success. If an aerobic sediment-water interface can be maintained, intermittent destratification removes cyanobacteria and prevents optimal development of other members of the photoautotrophic plankton. During growing seasons, increasing abundances of small-bodied herbivores (Bosmina) and Daphnia may have accounted for relatively low phytoplankton biomass as well. Intermittent destratification is a very fast-working in-lake measure and seems to be applicable even in relatively shallow lakes (< 15 m), in which permanent destratification seems to be risky.

The Fisheries for Mangrove Cockles,Anadaraspp., from Mexico to Peru, With Descriptions of Their Habitats and Biology, the Fishermen’s Lives, and the Effects of Shrimp Farming

This paper provides the first description of the mangrove cockle, Anadara spp., fisheries throughout their Latin American range along the Pacific coast from Mexico to Peru. Two species, A. tuberculosa and A. grandis, are found over the entire range, while A. similis occurs from El Salvador to Peru. Anadara tuberculosa is by far the most abundant, while A. grandis has declined in abundance during recent decades. Anadara tuberculosa and A. similis occur in level mud sediments in mangrove swamps, comprised mostly of Rhizophora mangle, which line the main-lands and islands of lagoons, whereas A. grandis inhabits intertidal mud flats along the edges of the same mangrove swamps. All harvested cockles are sexually mature. Gametogenesis of the three species occurs year round, and juvenile cockles grow rap-idly. Cockle densities at sizes at least 16–42 mm long ranged from 7 to 24/m2 in Mexico. Macrofaunal associates of cockles include crustaceans, gastropods, and finfishes. The mangrove swamps are in nearly pristine condition in every country except Honduras, Ecuador, and Peru, where shrimp farms constructed in the 1980’s and 1990’s have destroyed some mangrove zones. In addition, Hurricane Mitch destroyed some Honduran mangrove swamps in 1998. About 15,000 fishermen, including men, women, and children, harvest the cockles. Ecuador has the largest tabulated number of fishermen, 5,055, while Peru has the fewest, 75. Colombia has a large number, perhaps exceeding that in Ecuador, but a detailed census of them has never been made. The fishermen are poor and live a meager existence; they do not earn sufficient money to purchase adequate food to allow their full health and growth potential. They travel almost daily from their villages to the harvesting areas in wooden canoes and fiberglass boats at low tide when they can walk into the mangrove swamps to harvest cockles for about 4 h. Harvest rates, which vary among countries owing to differences in cockle abundances, range from about 50 cockles/fisherman/day in El Salvador and Honduras to 500–1,000/ fisherman/day in Mexico. The fishermen return to their villages and sell the cockles to dealers, who sell them mainly whole to market outlets within their countries, but there is some exporting to adjacent countries. An important food in most countries, the cockles are eaten in seviche, raw on the half-shell, and cooked with rice. The cockles are under heavy harvesting pressure, except in Mexico, but stocks are not yet being depleted because they are harvested at sizes which have already spawned. Also some spawning stocks lie within dense mangrove stands which the fishermen cannot reach. Consumers fortunately desire the largest cockles, spurning the smallest. Cockles are important to the people, and efforts to reduce the harvests to prevent overfishing would lead to severe economic suffering in the fishing communities. Pro-grams to conserve and improve cockle habitats may be the most judicious actions to take. Preserving the mangrove swamps intact, increasing their sizes where possible, and controlling cockle predators would lead to an increase in cockle abundance and harvests. Fishes that prey on juvenile cockles might be seined along the edges of swamps before the tide rises and they swim into the swamps to feed. Transplanting mangrove seedlings to suitable areas might increase the size of those habitats. The numbers of fishermen may increase in the future, because most adults now have several children. If new fishermen are tempted to harvest small, immature cockles and stocks are not increased, minimum size rules for harvestable cockles could be implemented and enforced to ensure adequate spawning.

The freshwater zooplankton of Sri Lanka, with a discussion of tropical freshwater zooplankton composition

Based on the study of over 500 zooplankton samples collected in Sri Lanka during 1965-1974, the species composition from different habitats is analysed. The zooplankton assemblage is typically tropical with relatively few species of Cladocera and Copepoda. The Rotifera include a large number of species of the genus Brachionus. The limnetic zooplankton resembles the pond zooplankton closely in that all the eurytopic species found in the different types of habitats, including ponds; also occur in the limnetic zooplankton. The large Cladocera belonging to the genus Daphnia are very rare. In general, large zooplankters are absent. The size composition of the zooplankton has a smaller range than in temperate regions. This is due to the absence of large-sized zooplankton species. The reasons for the differences in species variety and size composition between zooplankton of temperate and tropical regions is perhaps due to a number of factors. These include the effects of high and uniform temperatures, food availability and predation by fish and invertebrates.

Effect of two green algae species (Chlorella sp. & Scenedesmus obliquus) enriched with B group vitamins on food chain of Rutilus frisii kutum (Kamensky, 1901) fry

The Effect of two freshwater green algae species Chlorella sp. & Scenedesmus obliquus enriched (from the beginning of culture and after 96 hours) with different dosages of B group vitamins (0, 0.5, 1, and 2 ml of enriching solution per each liter of algae medium) on fecundity of Daphnia magna and growth of Rutilus frisii kutum fry were investigated in a research from spring, 2008 to autumn, 2009. First, each of the green algae species were cultured purely and massively in the Zander (Z-8+N) medium and then the nutritional value (the amount of protein, lipid, and carbohydrate) of enriched algae were meausered. In this study, enriching of Chlorella sp. & S. obliquus with a suitable mix of B group vitamins significantly improved their nutritive value. So the highest amount of nutritional value of Chlorella sp. was obtained because of enriching with dosage 0.5 ml.l-1 (366.654Kcal) and for Scenedesmus obliquus with dosage of 1 ml.l-1 (376.95Kcal). The acquired amount from control group showed an increase of respectively 42% and 11%. According to the results, increased dosages of enriching solution caused Daphnia fecundity to increase (at both stages : enrichment from the beginning of culture and after 96 hours). So the highest average of D. magna reproduction rate was obtained through being fed with Chlorella sp. and S. obliquus enriched with dosage of 2 ml enriching solution per liter of algae medium. The average fecundity of D. magna fed with Chlorella sp. enriched with dosage of 2 ml.l-1 enriching solution from the beginning of culture and after 96 hours was obtained respectively 2.128 ± 0.375 and 2.1 ± 0.69 and the average fecundity of D. magna fed with S. obliquus enriched with dosage of 2 ml enriching solution from the beginning of culture and after 96 hours was obtained respectively 2.128 ± 0.375 and 2.1 ± 0.69 which showed respectively an increase of 61 ٪, 91٪, 77 ٪, and 83٪ in proportion to the acquired amount from control group. When enriching solution was added to either algae culture medium from the beginning of culture, showed statistically significant differences (P<0.05) between dosages of 0 and 2 ml.l-1, 1 and 2 ml.l-1, and 0.5 and 2 ml enriching solution per each liter of Chlorella sp. culture medium and between dosages of 0 and 1 ml.l-1, and 0 and 2 ml enriching solution per each liter of S. obliquus culture medium. The highest average of body weight gain percentage and specific growth rate of kutum fry was obtained respectively 21.19%, 26.63%, 1.92, and 2.34 from the beginning of culture and after 96 hours with dosage of 1 ml B group vitamins per each liter of Chlorella sp. culture medium, which showed respectively an increase of 50%, 70%, 46%, and 62% in proportion to the acquired amount from control group. In the cases which Chlorella sp. were grown in the medium containing vitamin, from point of view of the average percentage of weight and specific growth rate of kutum fry significant differences were observed on the basis of the result of One-way ANOVA between dosages of 0 and 1, 1 and 2 , 0.5 and 1 ml B group vitamins per each liter. The highest average of body weight gain percentage and specific growth rate of kutum fry was obtained respectively 32.02%, 29.42%, 2.78, and 2.34 from the beginning of culture and after 96 hours with dosage of 2 ml B group vitamins per each liter of S. obliquus culture medium, which showed respectively an increase of 32%, 19%, 28%, and 17% in proportion to the acquired amount from control group. In the cases which S. obliquus were grown in the medium containing vitamin, from point of view of the average percentage of weight and specific growth rate of kutum fry significant differences were observed on the basis of the result of One-way ANOVA between dosages of 0 and 1, 0 and 2. According to the results of the present research we can say that considerable enhancement in the quality of the food of D. magna can be made by manipulation of the nutritional value of fresh water unicellular green algae with suitable mixture of B group vitamins, so that both the fecundity of D. magna will increase and the nutritional requirements of the kutum fry will be filled in this way.

Study of density, distribution and yield on benthic fauna and brown trout (Salmo trutta fario) feeding in Laar reservoirs

Lar lake, with the international UTM specification of 39S 579680 3976567 & 39S 589930 3976184 is Situated in Lar national Park with an aerial distance of 55 Km of Tehran along Haraz road. The present research is carried out as part of a comprehensives Plan for assessment of bioresearches of Lar lake & the rivers flowing into it. This research includes examination of there benthic Samplings performed in Lar lake and each of the related rivers including Delichaee, Ab-e-sefid , Alarm & Lar (Kamardasht).Tubifex and Chironomus genus were found to have the highest frequencies of occurrence in the lake with %77.117 & %21.823 respectively followed by Chironomidae and Simulidae from the Diptera order which accounted for %72.328 and %13.812 occurrences in four rivers examined in the Study. The benthic biomass at various examined Sites and the average wet weight of the benthic biomass in station No one in the lake Was 17.397g and the figure for the examined site in Alarm was 20.242 g which were the highest level among Other examination stations the index for the abundance of species in Alarm river was greater than the rest of the examined rivers with 12.57. A sum of 354 Pieces of brown trouts was caught in the course of sampling which were closely investigated in terms of their digestive tract Content. It was identified that Daphniidae and Chironomus constituted the bulk of eaten items from the lake with %17.985 and %63.973 respectively. Meanwhile, Chironomidae and Simuladae were the most frequently eanten benthos by the fish with %81.47 and %7.93 respectively.The index for the relative length of gut was recorded at 0.49± 0.08 which is well indicative of the carnivorous diet of the fish.The index for the feeding intensity amounted to 138  83 showing that the one year old fish were of more feeding intensity.The coefficient of condition (K) was estimated at 1.02  0.142 for all the caught fish. The average wet weight of the benthos was 10.348 g per square meter which if extended to 700ha surface area of the lake, the total macrobenthic production in the lake would amount to 72730Kg of wet weight or 6510 Kg of dry weight. Since the Secondary Production of macrobenthos have always been double that of their biomass, it is reasonable to assume that the Secondary Production of macrobenthos amount to 145640 Kg by their wet weight and Since the energy transfer in the food chain of the lake from benthos to fish is 10 percent, the fish production Capacity Coming from benthic resources of the lake (Lar) would be 14.5 MT, half of which (7000-8000MT) could annually be harvested. Further more, the actual fish Production Capacity might exceed the projected level Since Daphnia, Rotifers and Ostracoda which belong to Zooplanktons, play a part in the natural diet of trout. Meanwhile, rivers Play a major role in fish nutrition and the annual fish production in Delichaee river is about 4481.8Kg while the figures for Ab-e-sefid, Alerm and Lar rivers are 2370.7 4848.7 and 2586.2 Kg respectively, that further increase fish Production in the area and every year half of these resources can be exploitable from the river & the lake.Nevertheless, due to ecological & biological importance of rivers and the probability of environmental Pollution, devastation of natural fish habitats & their nursery grounds, Sport fishing is not recommended at all.

Assessing potential of production in Lake Choghakhor

Phytoplankton productivity is the common and important factor being considered in determining the overall status of a given body of water. This is because they are found at the base of an energy or food chain, being the basic source of primary food in a given aquatic system. Hence, information on their contribution is essential in indicating how much biomass energy will be available to all other living resources in the system. Though the primary productivity of shallow lakes is characterized by mixed populations of phytoplankton and submersed aquatic vegetation in the open water. Lake Choghakhor, is a shallow lake, located in Chaharmahal-Bakhtiyari Province. This lake is the most important ecosystem in the region especially for waterfowl populations, has a recreational value and supports tourism and fisheries. During last decade Choghakhor has been influenced by some man-made impacts such as water level fluctuation, agricultural discharge and fish (Cyprinids) introduction causing a serious problem in its trophic states. So water quality for physical, chemical and biological was monitored in five sampling stations, from April 2003 to March 2004. As biological parameters we studied phytoplankton, epiphytic algae, and zooplankton and macrobenthose community structure. Chlorophyll a content for phytoplankton and epiphytes was measured to estimate production of these groups (biomass over time). Also we determined biomasses of submersed macrophytes and macrobenthose and primary production of phytoplankton (dark and light bottles technique) to estimate fish production. The results of this study showed Lake Choghakhor did not undergo stable thermal and oxygen stratification, and the lake water was mixed throughout the study (the lake mixing regime is polymictic). Now submerged plants especially Myriophyllum spicatum has covered almost the entire lake and dense macrophyte beds (Polygonom amphibium), located on the east southern end of the lake appear to act as a sink for these nutrients. Lake Choghakhor appeared to be in a macrophyte dominated clear water state with low TP (annual mean: 24± 15μg.l-1) and chlorophyll a (annual mean: 3±1.28μg.l-1) concentrations and very high Secchi depth. The grazing pressure of dominant pelagic filtering zooplankton Daphnia longespina did not seem to be significant in determining the low phytoplankton crop expressed as chlorophyll a. We expect that sequestering of nutrients by submerged plants and associated epiphytes are the dominant stabilizing mechanisms suppressing the phytoplankton crop of Lake Choghakhor.

Effects of toxins induced by phytoplankton bloom on physiological function of indicator animals in Anzali Lagoon

The present overview summarizes data from one year's study during the period of 1379 to 1380 in the regions by "Anzali" lagoon called "Abkenar" and "Hendkhale". Specimens from this lagoon obtained weekly during mordad and shahrivar mounths (July 21 to september 21). The study included 67 types of 5 phytoplanktonal phylum. In "Abkenar" region Cyanobacters with maximum of 97% and minimum of 64.5% of total combination of phytoplanktones made the dominating combination during the period of study , while in "hendkhale" chyrsophyta with maximum of 89% and minimum of 38.7% of total phytoplanktonal was the dominating figure at the same period of time. Researches on ecological parameteres showed that the avarage dissolved oxygen in -Abkenar" and "hendlchale" regions was 10.7 and 8.0 mg/lit respectivly, also total rate of Phosphat in these regions was 0.085 and 0.15 mg/lit respectivly. This study showed that the rates of Nitrat and Amonium in "Abkenar" region was 0.043 and 0.79 mg / lit while for the same substances in "Ilendkhale" measured 0.08 and 0.7 mg I lit respective. Also the avarage rate of chlorophyll a in these two areas measured 58,38 and 40.45 j.un /ht respectivly. Depending on results of correlation cofficient in "Abkenar" region we had Cyanobacters , water and air temperature , Chlorophyll a and total amount of Phosphat as a poitive correlation with transparency while Amonium and Nitrat showed , a negative correlation , EC and finally dissolved oxygen showed a very low rate of correlation coffiocient. To perform this research 5 genus of Cyanobacteres horn "Anzali" lagoon have been isolated and cultured in a laboratorial conditions Later by using Mouse Bioassay method one of these genus identified as a toxic algae. Levels of LD50 with intra peritoneal injection of toxin on mouse in 24 hours was 660 mg/kg and Levels of LC50 by using Bioassay method on Artemia and Daphnia has been shown 618 and 1000 mg kg respectively. Also the physiological effects of were investigated. algae on two types of Cypronides family called Cyprinus carpi° and Hypophrthabnichthys Resultes of blood analyses of Cypronides who were feeded by toxic algae showed a significant decline (P < 0.05) in white and red blood cells and their hematocrites. Levels of LDH , SGOT and SGPT in their blood serum had a significant increase in porportion to control group (P < 0.05) but there was no evidance a differences in Total Protein levels. Pathological studies show damage and destruction of hepato pancreas and kidney of these fishes. Signs and symptoms of intoxication caused by Cyanobacter called Planktohrix agardlltii in mouse and fish show heptotoxic character. This toxin belongs to cyclicpeptides of microcystines group.

温度和培养体积对大型溞种群动态和两性生殖影响的研究

研究了5个培养体积(50、100、200、400、1000 mL)和2个温度(20℃、25℃)下大型溞(Daphnia magnaStraus)种群动态和两性生殖的变化,结果表明:(1)在相同培养体积下,随温度升高,大型溞首次抱卵时间缩短、首次产幼溞时间提前。在相同温度下,大型溞首次抱卵体长随培养体积的增大而增大。20℃和25℃下,1000 mL组大型溞首次抱卵体长分别是(2.54±0.02)mm和(2.71±0.01)mm。(2)在一定时间内,大型溞种群数量快速增加。但在较小培养体积下大型溞种群增长明显

两种不同株铜绿微囊藻培养液对大型溞的毒性效应研究

为了探讨铜绿微囊藻对浮游甲壳动物溞类的毒害效应,试验选取了两种不同株系(产毒和非产毒)的藻种,采取利用其培养液的方法,以研究铜绿微囊藻在生长过程中向水体中释放的毒素或类毒素物质对溞类的效应以及比较不同株系间效应的差异性。研究结果表明:(1)试验所选铜绿微囊藻产毒和非产毒株系的培养液均能对大型溞(Daphnia magna)的生长繁殖构成影响,表现为低剂量起促进效应,高剂量起抑制效应;(2)铜绿微囊藻产毒株较非产毒株更能影响大型溞的存活;(3)铜绿微囊藻产毒和非产毒株对大型溞的生长影响差异不大,但对其繁殖却

围隔中不同密度鲢对浮游植物的影响

试验采用2.5 m×2.5 m围隔,共设置4个试验鱼密度:0、116、176、和316 g/m2,研究了不同密度的鲢对浮游植物的影响。结果表明,仅在无鱼围隔中有大量个体较大的透明溞(Daphnia galeata)产生,并且在无鱼围隔中,浮游植物生物量最低,透明度显著的高于其他的围隔。与其它类似的围隔试验所不同的是:在无鱼围隔中没有蓝藻水华的发生。在有鱼围隔中,浮游甲壳动物始终由个体较小的微型裸腹溞占优势。并且浮游植物的生物量随着鲢密度的升高而降低。浮游植物的种类组成在不同鲢密度的围隔中比较相似,与无鱼围

用水毒理学方法评价家用洗涤剂的潜在危害

选用5种不同产地的常用类型的家用洗涤剂,用水毒理学方法研究它们对水生动物的毒性影响并由此判断对水生态系统的潜在危害.结果表明,在远低于日常使用量的浓度下,5种家用洗涤剂均对稀有鲫(Grobiocypris rarus)鱼苗和大型(Daphnia magna,HB)有急性毒性,离体条件下对鲫鱼(Carassius auratus)鳃ATP酶有较强的抑制作用;在无急性毒性浓度下暴露时对大型繁殖有明显影响;当暴露于家用洗涤剂对鱼24h半数致死浓度的5%时鲫鱼鳃ATP酶受到明显抑制;家用洗涤剂溶液存放一段时

略论武汉东湖枝角类种类演替及其与生态因子的关系

本文报道了武汉东湖1980—1988年间枝角类的种类演替,并分析了种类演替与主要生态因子间的关系,同时还与他人的研究结果进行了比较。研究结果表明,二十年来,枝角类种类组成已趋于简单,并由六十年代的47种减少为八十年代的26种。优势种群也发生了较大的变化。虽然冬、春季节仍以透明溞(Daphnia hyalina)为第一优势种,但以往夏、秋季节的优势种群隆线溞一亚种(Daphnia carinata ssp.)规模却日趋减少,并趋于消失;而杂食性的短尾秀体溞(Diaphanosoma brachyurum)种

一种耐高温大型溞HB的培养和生物学的初步研究

本文介绍了夏季在武汉鱼池所采的耐高温大型溞(Daphnia magna HB),对其生长繁殖及其实验室条件下溞的种群密度、食物、温度和光照强度对生长、发育的影响,并对其培养和保种方法进行了研究。连续3年实验室的培养表明,在以新鲜藻类为食物的情况下,此种溢在15—31℃时能正常生长、繁殖。在31℃时,其最大体长为3.65mm,最高怀卵量为26个。根据国际标准法组织所介绍的(ISO 6341)对标准毒物——重铬酸钾的敏感性试验,24hEC_(50)为0.92mg/L(20%)。

六六六对大型溞生态学的影响

在实验室条件下,测定了六六六对大型溞(Daphnia magna Straus)存活、生长和生殖的影响。在25℃时,以心跳停止为死亡标准,六六六(以丙体计)对大型溞48小时LC_(50)及其95%可信限为1.32±0.30ppm。以存活、生长和生殖为毒性标准,未觉察反应浓度(NOEC)为150ppb,最低觉察反应浓度(LOEC)为200ppb,其应用因子在0.11-0.15之间。内禀增长能力(r_m)是更为敏感的指标,六六六浓度要降低至50ppb才无明显影响。