Qualidade microbiológica da água e histopatologia de brânquias de peixes provenientes de pisciculturas do município de itapecuru-mirim, Estado do Maranhão

The present study evaluated the microbiological water quality and tissue lesions in gills from Nile tilapia (Oreochromis niloticus) and hybrid tambacu (Colossoma macropomum female x Piaractus mesopotamicus male). For this, water and gills were collected from fish farming at six locations in Itapecuru- Mirim County, Maranhão State. Microbiological water analyses revealed contamination by total coliforms, Escherichia coli and heterotrophic bacteria. In the gills, we observed a diversity of Gram-positive and Gramnegative bacteria. The tissue lesions were: lamellar fusion, interlamellar hyperplasia, sub-epithelial edema and telangiectasia. Inflammatory lesions were not observed. Significant statistical difference (p > 0.05) was not detected when comparing different gills lesions during rainy and dry season. The correlation between lesion and pond type was statistically different (p < 0.05) for lamellar fusion and interlamellar hyperplasia which occurred more frequently at ground ponds. Regarding the frequency of lesions in the different fish species, there was statistical difference (p < 0.05), and the tambacu was more sensitive to lamellar fusion while tilapia was more sensitive for the other lesions. In relation to the sampling stations, there was statistical difference for all the gill lesions. In conclusion, tissue lesions are nonspecific and function as a defense mechanism against polluted aquatic environments, without infectious character.

Mineral water: A microbiological approach

The microbiological quality of bottled mineral water of various domestic brands sold in Brazil was investigated, with particular focus on the heterotrophic plate count (HPC). Neither total coliforms nor Escherichia coli were found in any 1.5 L bottle samples. Total coliforms were found in 2.9% of the small bottles, while in 20 L bottles the presence of total coliforms and E. coli was demonstrated in 15.5 and 2.4% of samples, respectively. Pseudomonas aeruginosa was detected in 4.3, 4.5 and 9.5% of small, 1.5 and 20 L bottles, respectively. In 36.4% of the samples of 1.5 L bottles, the HPC was above 500 cfu/mL. This percentage of samples with an HPC above 500 cfu/mL increased to 52.0 and 61.9% in small and 20 L bottles, respectively. Higher contamination by total coliforms, E. coli, P. aeruginosa and HPCs occurred in 20 L bottles. In conclusion, several samples in this study were outside the international quality standard for mineral water and the large number of samples with high HPCs shows that more work must be done on the use of HPC in mineral water and the damaging effects that these microorganisms may cause to humans. The bottled mineral water was confirmed as a particularly important public health problem, due to the poor microbiological quality of the products that are marketed. © IWA Publishing 2012.

Caracterização de efluentes de viveiros de engorda de rã-touro (lithobates catesbeianus, Shaw, 1802)

Aim: Current analysis characterizes the effluent from bullfrog-rearing ponds during the grow-out phase; Methods: Temperature, pH, dissolved oxygen, electric conductivity, turbidity, total phosphorus, N-NH3, N-NO3, BOD5 and COD and the number of thermotolerant coliforms (Escherichia coli) of the inlet and outlet water of the ponds were analyzed twice a week. Assay consisted of a completely randomized experimental design with two treatments (inlet and outlet water) and six repetitions in a split-plot, coupled to collection over time as subplot; Results: All variables were significantly different (p < 0.05) between treatments and over time (p < 0.05). Average rates of temperature, pH and dissolved oxygen levels of the supply water were higher when compared to those of the effluent. The other variables such as conductivity, turbidity, total phosphorus, ammonia, nitrate, biological oxygen demand, chemical oxygen demand and E. coli were higher in the effluent when compared to rates in the supply water; Conclusions: The management during grow-out phase caused the deterioration of the water quality, with increasing levels of dissolved nutrients and the number of thermotolerant coliform. Ammonia and phosphorus levels in the effluent, caused by waste food, skin and feces, accelerate the eutrophication process of the receiving water body. Further studies on effluent treatment are required.

Uso do solo e monitoramento dos recursos hídricos no córrego do Ipê, Ilha Solteira, SP

Brazil has great potential of water resources, however, with deficiency in terms of qualitative and quantitative monitoring and knowledge of the influences of land use. So, this study had the purpose of presenting the qualitative and quantitative monitoring of water for irrigation and the influences caused by the use and occupation of land in the watershed of Ipê stream, in the municipality of Ilha Solteira, São Paulo. The qualitative monitoring was conducted from 2006 to 2011 and the quantitative monitoring from 2009 to 2011. In order to evaluate the influences, the Pearson correlation analysis was used. It was found that the watershed is mostly made up by the crop of sugar cane and the use in conflict with areas of permanent preservation are the fallow areas and pasture. The watershed is characterized with high concentrations of iron and coliforms, originating from degraded pastures and irregular use of urban and rural occupation. The small permanent preservation areas, bad soil conservation and expansion of urban areas imply in the deterioration of the quality and availability of water, generating socio-economic and environmental impact in the area.

Microbiological quality of organic and conventional vegetables sold in Brazil

While searching for healthier diets, people became more attentive to organic produce. Yet, organic foods may be more susceptible to microbiological contamination because of the use of organic fertilizers, a possible source of pathogenic bacteria. In this study, 130 samples of different organic and conventional vegetable varieties sold in Brazil were analyzed for mesophilic aerobic bacteria, yeasts and molds, total coliforms, Escherichia coli and Salmonella spp. Most of the mesophilic aerobic bacteria counts in organic and conventional vegetables ranged from 6 to 7 log10 CFU/g; most of the yeasts and molds counts ranged from 5 to 6 log10 CFU/g and most of the total coliforms counts ranged from 4 to 5 log10 CFU/g. E. coli was found in 41.5% of the organic and 40.0% of the conventional vegetables, and most samples had counts ranging from 1 to 2 log10 CFU/g. Salmonella spp. was not found in any sample. Comparative analyses of the microbial counts of organic and conventional vegetables showed that some organic varieties have greater counts. However, the global results show that this is not a trend. These results indicate the need of good farming practices, and proper sanitization before consumption, to ensure food quality and safety. © 2012 Elsevier Ltd.

Avaliação microbiológica do camarão da Amazônia (Macrobrachium amazonicum) e sua relação com o ambiente de criação na carcinicultura

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Qualidade higiênica e sanitária de tilápias provenientes de cultivo, comercializadas no varejo

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Estudos limnológicos de balneabilidade no Parque Natural Municipal Cachoeira da Marta (Botucatu, SP, Brasil): relação com possíveis fontes poluidoras

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Perfil microbiológico de peixes e água de cultivo em pesque-pagues situados na região nordeste do Estado de São Paulo

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Qualidade microbiológica do ambiente e da tilápia-do-Nilo (Oreochromis niloticus) produzida em sistema de tanques-rede no reservatório de Chavantes, SP

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação do uso de inóculos na biodigestão anaeróbia de resíduos de aves de postura, frangos de corte e suínos

Pós-graduação em Biotecnologia - IQ

Avaliação da eficiência de métodos rápidos usados para detecção de coliforme totais e coliforme fecais em amostras de água, em comparação com a técnica de fermentação em tubos múltiplos

Pós-graduação em Alimentos e Nutrição - FCFAR

Condições higiênico-sanitárias de alimentos prontos para o consumo servidos em escolas públicas de um município da região noroeste do Estado de São Paulo e perfil de sensibilidade das cepas de Staphylococcus aureus e Escherichia coli isoladas frente a agentes antimicrobianos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Perfil microbiano, físico-químico e análise das boas práticas de fabricação (BPF) de queijos minas frescal e ricota

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Qualidade microbiológica e ocorrência de leveduras em bebidas lácteas fermentadas, com adição de polpas de frutas, comercializada na região de São José do Rio Preto - SP

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)