Further Concerns About Glutamine: A Case Report on Hyperammonemic Encephalopathy.

OBJECTIVE We report a case of a woman with hyperammonemic encephalopathy following glutamine supplementation. DESIGN Case report. INTERVENTIONS Plasma amino acid analysis suggestive of a urea cycle defect and initiation of a treatment with lactulose and the two ammonia scavenger drugs sodium benzoate and phenylacetate. Together with a restricted protein intake ammonia and glutamine plasma levels decreased with subsequent improvement of the neurological status. MEASUREMENTS AND MAIN RESULTS Massive catabolism and exogenous glutamine administration may have contributed to hyperammonemia and hyperglutaminemia in this patient. CONCLUSION This case adds further concerns regarding glutamine administration to critically ill patients and implies the importance of monitoring ammonia and glutamine serum levels in such patients.

Premature Discontinuation of Randomized Trials in Critical and Emergency Care: A Retrospective Cohort Study.

OBJECTIVES Randomized clinical trials that enroll patients in critical or emergency care (acute care) setting are challenging because of narrow time windows for recruitment and the inability of many patients to provide informed consent. To assess the extent that recruitment challenges lead to randomized clinical trial discontinuation, we compared the discontinuation of acute care and nonacute care randomized clinical trials. DESIGN Retrospective cohort of 894 randomized clinical trials approved by six institutional review boards in Switzerland, Germany, and Canada between 2000 and 2003. SETTING Randomized clinical trials involving patients in an acute or nonacute care setting. SUBJECTS AND INTERVENTIONS We recorded trial characteristics, self-reported trial discontinuation, and self-reported reasons for discontinuation from protocols, corresponding publications, institutional review board files, and a survey of investigators. MEASUREMENTS AND MAIN RESULTS Of 894 randomized clinical trials, 64 (7%) were acute care randomized clinical trials (29 critical care and 35 emergency care). Compared with the 830 nonacute care randomized clinical trials, acute care randomized clinical trials were more frequently discontinued (28 of 64, 44% vs 221 of 830, 27%; p = 0.004). Slow recruitment was the most frequent reason for discontinuation, both in acute care (13 of 64, 20%) and in nonacute care randomized clinical trials (7 of 64, 11%). Logistic regression analyses suggested the acute care setting as an independent risk factor for randomized clinical trial discontinuation specifically as a result of slow recruitment (odds ratio, 4.00; 95% CI, 1.72-9.31) after adjusting for other established risk factors, including nonindustry sponsorship and small sample size. CONCLUSIONS Acute care randomized clinical trials are more vulnerable to premature discontinuation than nonacute care randomized clinical trials and have an approximately four-fold higher risk of discontinuation due to slow recruitment. These results highlight the need for strategies to reliably prevent and resolve slow patient recruitment in randomized clinical trials conducted in the critical and emergency care setting.

Chronology for the EDML ice core from Dronning Maud Land, Antarctica, over the last 150 000 years

A chronology called EDML1 has been developed for the EPICA ice core from Dronning Maud Land (EDML). EDML1 is closely interlinked with EDC3, the new chronology for the EPICA ice core from Dome-C (EDC) through a stratigraphic match between EDML and EDC that consists of 322 volcanic match points over the last 128 ka. The EDC3 chronology comprises a glaciological model at EDC, which is constrained and later selectively tuned using primary dating information from EDC as well as from EDML, the latter being transferred using the tight stratigraphic link between the two cores. Finally, EDML1 was built by exporting EDC3 to EDML. For ages younger than 41 ka BP the new synchronized time scale EDML1/EDC3 is based on dated volcanic events and on a match to the Greenlandic ice core chronology GICC05 via 10Be and methane. The internal consistency between EDML1 and EDC3 is estimated to be typically ~6 years and always less than 450 years over the last 128 ka (always less than 130 years over the last 60 ka), which reflects an unprecedented synchrony of time scales. EDML1 ends at 150 ka BP (2417 m depth) because the match between EDML and EDC becomes ambiguous further down. This hints at a complex ice flow history for the deepest 350 m of the EDML ice core.

Effects of increased CO2 concentration on nutrient limited coastal summer plankton depend on temperature

Increasing seawater temperature and CO2 concentrations both are expected to increase coastal phytoplankton biomass and carbon to nutrient ratios in nutrient limited seasonally stratified summer conditions. This is because temperature enhances phytoplankton growth while grazing is suggested to be reduced during such bottom-up controlled situations. In addition, enhanced CO2 concentrations potentially favor phytoplankton species, that otherwise depend on costly carbon concentrating mechanisms (CCM). The trophic consequences for consumers under such conditions, however, remain little understood. We set out to experimentally explore the combined effects of increasing temperature and CO2 concentration for phytoplankton biomass and stoichiometry and the consequences for trophic transfer (here for copepods) on a natural nutrient limited Baltic Sea summer plankton community. The results show, that warming effects were translated to the next trophic level by switching the system from a bottom-up controlled to a mainly top-down controlled one. This was reflected in significantly down-grazed phytoplankton and increased zooplankton abundance in the warm temperature treatment (22.5°C). Additionally, at low temperature (16.5°C) rising CO2 concentrations significantly increased phytoplankton biomass. The latter effect however, was due to direct negative impact of CO2 on copepod nauplii which released phytoplankton from grazing in the cold but not in the warm treatments. Our results suggest that future seawater warming has the potential to switch trophic relations between phytoplankton and their grazers under nutrient limited conditions with the consequence of potentially disguising CO2 effects on coastal phytoplankton biomass.

Living benthic foraminifers in bottom sediments of the southeastern Kandalaksha Bay (White Sea)

Analysis of composition and distribution of benthic foraminifers in six samples of bottom sediments obtained in the southeast Kandalaksha Bay of the White Sea at water depths of 20 to 155 m revealed their dependence on lithology and different hydrological characteristics. It is shown that living foraminifers populating relatively shallow areas of the bay (20-60 m), which are bathed by seasonally warmed intermediate water with temperature 0.7-1.5°C and salinity 26 per mil, are characterized by high abundance (250-750 specimens/10 ccm of wet sediment) and prevalence of agglutinated species (Eggerella advena, Recurvoides turbinatus, and others). Deeper (155 m) where cold and relatively saline deep water occurs (-1.4°C, 29.5 per mil), abundance is an order lower (30 specimens/10 ccm) and is dominated by calcareous taxa Cassidulina reniforme, an Arctic cold resistant species.

Age determination of the rhyolites of the Lord Howe Rise in the Southwest Pacific Ocean

Drilling at site 207 (DSDP Leg 21), located on the broad summit of the Lord Howe Rise, bottomed in rhyolitic rocks. Sanidine concentrates from four samples of the rhyolite were dated by the 40Ar/39Ar total fusion method and conventional K-Ar method, and yielded concordant ages of 93.7 +/- 1.1 my, equivalent to the early part of the Upper Cretaceous. At this time the Lord Howe Rise, which has continental-type structure, is thought to have been emergent and adjacent to the eastern margin of the Australian-antarctic continent. Subsequent to 94 my ago and prior to deposition of Maastrichtian (70-65 myBP) marine sediments on top of the rhyolitic basement of the Lord Howe Rise, rifting occurred and the formation of the Tasman Basin began by sea-floor spreading with rotation of the Rise away from the margin of Australia. Subsidence of the Rise continued until Early Eocene (about 50 myBP), probably marking the end of sea-floor spreading in the Tasman Basin. These large scale movements relate to the breakup of this part of Gondwanaland in the Upper Cretaceous.

Miocene radiolarians of the Sulu Sea

Radiolarians are sporadic in sediments collected in the Sulu Sea during ODP Leg 124. Due to the generally poor preservation and low abundance of radiolarians in Sulu Sea sediments, no biostratigraphic datums are well defined, although three radiolarian zones are identified. Most samples containing radiolarians are pelagic or hemipelagic clays with varying proportions of volcanic ash. Detailed analysis of Sulu Sea radiolarians was limited to Miocene successions. Pliocene and Quaternary occurrences of radiolarians were noted but have not been zoned. The late middle Miocene of Sites 769 and 771 is represented by an assemblage of radiolarians (Diartus petterssoni Zone) that is entirely replaced by massive pyrite. This type of preservation develops only under anoxic conditions. The development of widespread anoxia in Sulu Sea waters in the late middle Miocene was probably the result of hydrologic isolation of basin waters, and may be associated with eustatic sea level fall over the silled basin. Upper lower Miocene pelagic and hemipelagic sediments that overlie pyroclastics and basalt flows in the Sulu Sea sites contain moderately to very poorly preserved radiolarians of the Calocycletta costata Zone. A thin unit of marine claystone was recovered from between the thick pyroclastics and basement rocks at Site 768. Radiolarians present in these claystones are rare and very poorly preserved. This radiolarian assemblage probably represents the C. costata Zone, although very poor preservation and low abundance make this interpretation equivocal. The radiolarian zones identified constrain the age of basin formation to late early Miocene or earlier.

Lower Cretaceous radiolarians from the northwestern Australian margin

During Leg 123, abundant and well-preserved Neocomian radiolarians were recovered at Site 765 (Argo Abyssal Plain) and Site 766 (lower Exmouth Plateau). The assemblages are characterized by a scarcity or absence of Tethyan taxa. The Berriasian-early Aptian radiolarian record recovered at Site 765 is unique in its density of well-preserved samples and in its faunal contents. Remarkable contrasts exist between radiolarian assemblages extracted from claystones of Site 765 and reexamined DSDP Site 261, and faunas recovered from radiolarian sand layers of Site 765. Clay faunas are unusual in their low diversity of apparently ecologically tolerant species, whereas sand faunas are dominated by non-Tethyan species that have never been reported before. Comparisons with Sites 766 and 261, as well as sedimentological observations, lead to the conclusion that this faunal contrast results from a difference in provenance, rather than from hydraulic sorting. Biostratigraphic dating proved difficult principally because of the paucity or even absence of (Tethyan) species used in published zonations. In addition, published zonations are contradictory and do not reflect total ranges of species. Radiolarian assemblages recovered from claystones at Sites 765 and 261 in the Argo Basin reflect restricted oceanic conditions for the latest Jurassic to Barremian time period. Neither the sedimentary facies nor the faunal associations bear any resemblance to sediment and radiolarian facies observed in typical Tethyan sequences. I conclude that the Argo Basin was paleoceanographically separated from Tethys during the Late Jurassic and part of the Early Cretaceous by its position at a higher paleolatitude and by enclosing landmasses, i.e., northeastern India and the Shillong Block, which were adjacent to the northwestern Australian margin before the opening. Assemblages recovered from radiolarian sand layers are dominated by non-Tethyan species that are interpreted as circumantarctic. Their sudden appearance in the late Berriasian/early Valanginian pre-dates the oceanization of the Indo-Australian break-up (Ml 1, late Valanginian) by about 5 m.y., but coincides with a sharp increase in margin-derived pelagic turbidites. The Indo-Australian rift zone and its adjacent margins probably were submerged deeply enough to allow an intermittent "spillover" of circumantarctic cold water into the Argo Basin, creating increased bottom current activity. Circumantarctic cold-water radiolarians transported into the Argo Basin upwelled along the margin and died en masse. Concomitant winnowing by bottom currents led to their accumulation in distinct radiolarite layers. High rates of faunal change and the sharp increase of bottom current activity are thought to be synchronous with the two pronounced late Berriasian-early Valanginian lowstands in sea level. Hypothetically, both phenomena might have been caused by a glaciation on the Antarctic-Australian continent, which was for the first time isolated from the rest of Gondwana by oceanic seaways as a result of Jurassic and Early Cretaceous seafloor spreading. The absence of typical Tethyan radiolarian species during the late Valanginian to late Hauterivian period is interpreted as reflecting a time of strong influx of circumantarctic cold water following oceanization (Mil) and rapid spreading between southeast India and western Australia. The reappearance and gradual increase in abundance and diversity of Tethyan forms along with the still dominant circumantarctic species are thought to result from overall more equitable climatic conditions during the Barremian and early Aptian and may have resulted from the establishment of an oceanic connection with the Tethys Ocean during the early Aptian.

A laboratory-scale experiment and a numerical simulation of unusual spiral plumes in a High-Prandtl-number fluid, links supplementary material

We experimentally and numerically investigated the generation of plumes from a local heat source (LHS) and studied the interaction of these plumes with cellular convective motion (CCM) in a rectangular cavity filled with silicon oil at a Prandtl number (Pr) of approximately two thousand. The LHS is generated using a 0.2-W green laser beam. A roll-type CCM is generated by vertically heating one side of the cavity. The CCM may lead to the formation of an unusual spiral convective plume that resembles a vertical Archimedes spiral. A similar plume is obtained in a direct numerical simulation. We discuss the physical mechanism for the formation of a spiral plume and the application of the results to mantle convection problems. We also estimate the Reynolds (Re) and Rayleigh (Ra) numbers and apply self-similarity theory to convection in the Earth's mantle. Spiral plumes can be used to interpret mantle tomography results over the last decade.

Basalt density, basement age, and intrusive/extrusive relations from DSDP Legs 2 through 7, 9, and 14

Densities of layer 2 basalt recovered during the Deep Sea Drilling Project have been found to decrease steadily with age, a finding ascribed to progressive submarine weathering in the context of sea-floor spreading. The least-squares solution for 52 density measurements gives a rate of decrease in density of (Delta p)/(Delta t) = -0.0046 g per ccm m.y. = -16 percent per 100 m.y., which is in excellent agreement with earlier estimates based on observed chemical depletion rates of dredged oceanic basalt. Weathering of sea-floor basalt, should it penetrate to any considerable depth in layer 2, will decrease layer 2 seismic refraction velocities, act as a source of geothermal heat, and substantially influence the chemistry of sea water and the overlying column of sediment.

Ocean acidification alleviates low-temperature effects on growth and photosynthesis of the red alga Neosiphonia harveyi (Rhodophyta)

This study aimed to examine interactive effects between ocean acidification and temperature on the photosynthetic and growth performance of Neosiphonia harveyi. N. harveyi was cultivated at 10 and 17.5 °C at present (~380 µatm), expected future (~800 µatm), and high (~1500 µatm) pCO2. Chlorophyll a fluorescence, net photosynthesis, and growth were measured. The state of the carbon-concentrating mechanism (CCM) was examined by pH-drift experiments (with algae cultivated at 10 °C only) using ethoxyzolamide, an inhibitor of external and internal carbonic anhydrases (exCA and intCA, respectively). Furthermore, the inhibitory effect of acetazolamide (an inhibitor of exCA) and Tris (an inhibitor of the acidification of the diffusive boundary layer) on net photosynthesis was measured at both temperatures. Temperature affected photosynthesis (in terms of photosynthetic efficiency, light saturation point, and net photosynthesis) and growth at present pCO2, but these effects decreased with increasing pCO2. The relevance of the CCM decreased at 10 °C. A pCO2 effect on the CCM could only be shown if intCA and exCA were inhibited. The experiments demonstrate for the first time interactions between ocean acidification and temperature on the performance of a non-calcifying macroalga and show that the effects of low temperature on photosynthesis can be alleviated by increasing pCO2. The findings indicate that the carbon acquisition mediated by exCA and acidification of the diffusive boundary layer decrease at low temperatures but are not affected by the cultivation level of pCO2, whereas the activity of intCA is affected by pCO2. Ecologically, the findings suggest that ocean acidification might affect the biogeographical distribution of N. harveyi.

Seawater carbonate chemistry, growth rate and light sensitivity of marine diatom Chaetoceros muelleri (strain CSIRO CS-176) during experiments, 2011

This study investigated the impact of photon flux and elevated CO2 concentrations on growth and photosynthetic electron transport on the marine diatom Chaetoceros muelleri and looked for evidence for the presence of a CO2-concentrating mechanism (CCM). pH drift experiments clearly showed that C. muelleri has the capacity to use bicarbonate to acquire inorganic carbon through one or multiple CCMs. The final pH achieved in unbuffered cultures was not changed by light intensity, even under very low photon flux, implying a low energy demand of bicarbonate use via a CCM. In short-term pH drift experiments, only treatment with the carbonic anhydrase inhibitor ethoxyzolamide (EZ) slowed down the rise in pH considerably. EZ was also the only inhibitor that altered the final pH attained, although marginally. In growth experiments, CO2 availability was manipulated by changing the pH in closed flasks at a fixed dissolved inorganic carbon (DIC) concentration. Low-light-treated samples showed lower growth rates in elevated CO2conditions. No CO2 effect was recorded under high light exposure. The maximal photosynthetic capacity, however, increased with CO2 concentration in saturating, but not in subsaturating, light intensities. Growth and photosynthetic capacity therefore responded in opposite ways to increasing CO2 availability. The capacity to photoacclimate to high and low photon flux appeared not to be affected by CO2treatments. However, photoacclimation was restricted to growth photon fluxes between 30 and 300 µmol photons m-2 s-1. The light saturation points for photosynthetic electron transport and for growth coincided at 100 µmol photons m-2 s-1. Below 100 µmol photons m-2 s-1 the light saturation point for photosynthesis was higher than the growth photon flux (i.e. photosynthesis was not light saturated under growth conditions), whereas at higher growth photon flux, photosynthesis was saturated below growth light levels.

Physiological responses of the marine diatom Thalassiosira pseudonana to increased pCO2 and seawater acidity

We studied the effects of elevated CO2 concentration and seawater acidity on inorganic carbon acquisition, photoinhibition and photoprotection as well as growth and respiration in the marine diatom Thalassiosira pseudonana. After having grown under the elevated CO2 level (1000 µatm, pH 7.83) at sub-saturating photosynthetically active radiation (PAR, 75 µmol photons/m**2/s) for 20 generations, photosynthesis and dark respiration of the alga increased by 25% (14.69 ± 2.55 fmol C/cell/h) and by 35% (4.42 ± 0.98 fmol O2/cell/h), respectively, compared to that grown under the ambient CO2 level (390 µatm, pH 8.16), leading to insignificant effects on growth (1.09 ± 0.08 (1/d))v 1.04 ± 0.07 (1/d)). The photosynthetic affinity for CO2 was lowered in the high-CO2 grown cells, reflecting a down-regulation of the CO2 concentrating mechanism (CCM). When exposed to an excessively high level of PAR, photochemical and non-photochemical quenching responded similarly in the low- and high-CO2 grown cells, reflecting that photoinhibition was not influenced by the enriched level of CO2. In T. pseudonana, it appeared that the energy saved due to the down-regulated CCM did not contribute to any additional light stress as previously found in another diatom Phaeodactylum tricornutum, indicating differential physiological responses to ocean acidification between these two diatom species.

Radiolarians of the Celebes Sea

Two sites were drilled in the Celebes Sea as part of Ocean Drilling Program Leg 124; Site 767 and Site 770. Radiolarians are preserved in Paleogene pelagic claystones with minor occurrences in certain Neogene successions. The brown clays that immediately overlie basalt at both sites contain radiolarians of the late middle Eocene Podocyrtis chalara Zone. Late Eocene radiolarians are not found, due to dissolution and probable hiatus. The Oligocene is represented by the Theocyrtis tuberosa and Dorcadospyris ateuchus Zones. Oligocene sediments are strongly dominated by abundant and diverse radiolarians of the TristylospyrislDorcadospyris lineage. Preservation of Paleogene radiolarian assemblages ranges from good to very poor. Late Miocene radiolarians of the Didymocyrtis antepenultima Zone are found only in Site 770. Other Neogene sediments are barren of radiolarian remains, with the exception of latest Pleistocene and Holocene sediments.