Prevalence study of SNP c.421G>T in the ADAMTS2 gene responsible for dermatosparaxis in White Dorper sheep in Brazil

Abstract: Dermatosparaxis is an autosomal recessive disorder of connective tissue; the disorder is clinically characterized by skin fragility and hyperextensibility. Dermatosparaxis in White Dorper sheep is caused by a single nucleotide polymorphism (SNP) (c.421G>T) in the ADAM metalloproteinase with thrombospondin type 1 motif, 2 (ADAMTS2) gene. The aim of this study was to investigate the prevalence of this SNP in a White Dorper herd in São Paulo state, Brazil. In this study, we collected blood DNA samples from 303 White Dorper sheep and performed polymerase chain reaction to amplify the SNP region. The samples were sequenced to determine the presence of the SNP in the ADAMTS2 gene. The SNP prevalence in the studied population was 15.5%; this finding indicates that more effective control measures should be used to prevent the inheritance of SNP c.421G>T in the ADAMTS2 gene in Brazilian White Dorper herds.

Hunting for differentially expressed genes

Differentially expressed genes are usually identified by comparing steady-state mRNA concentrations. Several methods have been used for this purpose, including differential hybridization, cDNA subtraction, differential display and, more recently, DNA chips. Subtractive hybridization has significantly improved after the polymerase chain reaction was incorporated into the original method and many new protocols have been established. Recently, the availability of the well-known coding sequences for some organisms has greatly facilitated gene expression analysis using high-density microarrays. Here, we describe some of these modifications and discuss the benefits and drawbacks of the various methods corresponding to the main advances in this field.

Evaluation of Petrifilm™ system compared with traditional methodology in count of indicators of sanitary-hygienic quality and pathogenic microorganisms in sheep milk

The objective of this study was to evaluate the applicability of the Petrifilm™ plates to enumerate microbial groups in sheep milk. Samples of sheep milk (n = 30) were plated simultaneously, to enumerate mesophilic aerobes, total coliforms, lactic acid bacteria, Staphylococcus aureus and Escherichia coli, using convencional reference protocols and Petrifilm™ plates. The results were compared using McNemar's test, linear regression and ANOVA (p < 0,05). The results demonstrated good significant between conventional methodologies and Petrifilm™ plates. Further, the Petrifim™ STX for counting S. aureus had higher recoverability of bacteria compared with the conventional methodology. Based on the results obtained and in view of the ease and rapidity procedures results, Petrifim ™ plates may be considered as alternatives for microbiological testing in sheep milk.

Photograph - Group Return from Hunting

A photograph of a male holding a rifle by his side, with four females (varying ages) and another male is standing in the background. They are holding a long stick which is carrying their game.

Photograph - Percy Band Hunting with others

A photograph of Percy Band with a group of men with a pack of hunting dogs.

Photograph - Percy Band Hunting with others #2

Another photograph of the Percy Band with a group of hunters.

Photograph - Hunting Group with Percy Band

A group of men with rifles and hunting dogs. The photograph is dark and cloudy making it difficult to see details. Percy Band is sitting in front.

Samuel DeVeaux Woodruff's Pewter Hunting Flask

This is a 16 cm x 8 cm 19th century pewter flask which has 3 pieces including the flask, the screw-on cap and a cover/drinking cup. There is a W inscribed within the cap. This is well-worn and dented.

Explorations of identity in Philip K. Dick's Do androids dream of electric sheep?

Mémoire numérisé par la Division de la gestion de documents et des archives de l'Université de Montréal

The Koundoum sheep breed in Niger: morpho-biometric study and description of the production system

The diffusion of highly productive breeds across developing countries goes along with a neglect of indigenous breeds, which are well suited to their environment but often show low yields. Thus, in Niger, the flock of Koundoum sheep are rapidly decreasing. The Koundoum is one of the few wool sheep breeds of Africa and shows important adaptive feature to its native environment, i.e. the humid pastures on the banks of the Niger River. To characterise the breed and to understand its production context, a survey has been conducted in 104 herds in four communes along the Niger River (Kollo, Tillabery, Say and Tera). Nine body measurements, including live weight, were taken on 180 adult sheep (101 females and 79 males). The herds varied from 2 to 60 heads, with a median size of eight animals and two thirds of the herds having less than 10 animals. Mainly fed on natural pastures, 85.6% of the herds received crop residues. Only natural mating was practiced. Veterinary care was restricted to anti-helminthic and some indigenous treatments. The frequent affiliation of breeders to professional unions appeared as favourable to the implementation of a collective conservation program. The Koundoum sheep were white or black coated, with the black colour being most frequent (75.6%). Wattles were present in both sexes at similar frequencies of around 14%. All biometric variables were significantly and positively correlated between them. The thoracic perimeter showed the best correlation with live weight in both males and females. Three variables were selected for live weight prediction: thoracic perimeter, height at withers and rump length. From the present study, it is expected that the in situ conservation of the Koundoum sheep will be highly problematic, due to lack of market opportunities for wool and the willingness of smallholders to get involved in pure Koundoum rearing.

Annabelle the sheep

This is a part of a collection of materials developed by the HEAcademy Subject Centre for Languages, linguistics and area studies. The materials provide reflective activities designed to engage teachers with some of the key issues in working with international students and practical ideas for ways in which these can be addressed. They will be of particular interest to new staff or anyone new to working with international students.

Woolly the Sheep.

Un cuento de granja para los más pequeño donde se relata las aventuras de la oveja Woolly. Las ilustraciones permiten comentar fácilmente la historia para ampliar el lenguaje del niño.

Russell the sheep.

Mientras que las otras ovejas duermen, Russell tiene problemas de insomnio. Intenta dormir en diferentes lugares: un oxidado automóvil, un árbol hueco, una rama alta. Cuando nada funciona, intenta contar cosas: las piernas, las estrellas y, por último, ovejas.

The wolf in sheep's clothing : and other fables from Aesop.

Alrededor de seis siglos antes de Cristo, Esopo, un esclavo griego, hizo una recopilación de historias o fábulas que han permanecido hasta hoy en los cuentos populares. El lobo con piel de cordero es una de las seis historias que aparecen en esta colección y como todas las fábulas cuentan con una moral, que ofrece al lector consejos útiles que se resume en un proverbio al final. Otras fábulas de este libro incluyen: Las ranas que querían un Rey, El zorro y la cigüeña y otras más.