An Evening with Lisa Nicole Bell, Award-Winning Media Entrepreneur, Writer, Producer, and On Camera Talent

Lisa Bell is the founder and CEO of Inspired Life Media Group, a Los Angeles-based international content development and production company that works with a diverse cross-section of venture-backed startups, high profile individuals, and fortune 500 brands. Lisa, past content and business ventures are critically-acclaimed, diverse, and plentiful. She created, directed, and produced The American Dream Revised, a digital docuseries that follows a diverse group of young entrepreneurs. Lisa’s past business ventures include startups in technology, personal development, and original content.After launching her first company at 19 years old, she later started a for-profit social enterprise that reached more than 400,000 girls around the world with active programs in Liberia, England, Brazil, and the US.

A Universidade de bolonha e a cultura universal os herdeiros espirituais de bolonha no ocidente : andres bell / Silvio A. B. Meira. --

Trabalho apresentado na Universidade Nacional do Chile, Santiago, em comemoração aos 900 anos da Universidade de Bolonha.

Red Bell Pepper Chromoplasts Exhibit in Vitro Import Competency and Membrane Targeting of Passenger Proteins from the Thylakoidal Sec and ΔpH Pathways but Not the Chloroplast Signal Recognition Particle Pathway1

Chloroplast to chromoplast development involves new synthesis and plastid localization of nuclear-encoded proteins, as well as changes in the organization of internal plastid membrane compartments. We have demonstrated that isolated red bell pepper (Capsicum annuum) chromoplasts contain the 75-kD component of the chloroplast outer envelope translocon (Toc75) and are capable of importing chloroplast precursors in an ATP-dependent fashion, indicating a functional general import apparatus. The isolated chromoplasts were able to further localize the 33- and 17-kD subunits of the photosystem II O2-evolution complex (OE33 and OE17, respectively), lumen-targeted precursors that utilize the thylakoidal Sec and ΔpH pathways, respectively, to the lumen of an internal membrane compartment. Chromoplasts contained the thylakoid Sec component protein, cpSecA, at levels comparable to chloroplasts. Routing of OE17 to the lumen was abolished by ionophores, suggesting that routing is dependent on a transmembrane ΔpH. The chloroplast signal recognition particle pathway precursor major photosystem II light-harvesting chlorophyll a/b protein failed to associate with chromoplast membranes and instead accumulated in the stroma following import. The Pftf (plastid fusion/translocation factor), a chromoplast protein, integrated into the internal membranes of chromoplasts during in vitro assays, and immunoblot analysis indicated that endogenous plastid fusion/translocation factor was also an integral membrane protein of chromoplasts. These data demonstrate that the internal membranes of chromoplasts are functional with respect to protein translocation on the thylakoid Sec and ΔpH pathways.