Effect of age and breeding season on the developmental capacity of oocytes from unstimulated and follicle-stimulating hormone-stimulated rhesus monkeys

Effects of age and season on the developmental capacity of oocytes from unstimulated and FSH-stimulated rhesus monkeys were examined, Immature cumulus-oocyte complexes were matured in vitro in modified CMRL-1066 medium containing 20% bovine calf serum and

Effect of carp PG doses on induced breeding of Shing, Heteropneustes fossilis (Bloch)

Five hormone doses viz. 25, 50, 75, 100, and 125 mg of carp PG/kg of body weight of the recipient fish were tested and they were designated as T1 T2, T3, T4, and T5 respectively. Significantly higher fertilization (98%) and hatching rates (38%) were obtained from T3 (75 mg of carp PG extract/kg body weight). While T4 (100 mg of carp PG extract/kg body weight) and T5 (125 mg of carp PG extract/kg body weight) gave the highest (90%) ovulation rate. In June and July the highest fertilization rate of 96 and 96.4% respectively and hatching rate 42.5 and 48.7% respectively were obtained. In over all consideration carp PG extract at a dose of 75 mg/kg body weight appears to be the suitable dose for induced breeding of H. fossilis and June and July are the suitable time for its induced breeding.

Experiments on the induced breeding of milkfish, Chanos chanos (Forsskal) in 1978

Results indicated that a female having eggs with an average diameter of < 0.7 mm did not respond well to the hormone injections.

Induced breeding of Indian carps using Des Gly^10 [D-Ala^6] LHRH ethylamide

Details are given of a study conducted in order to determine the efficacy of Des Gly^10 [D-Ala^6] LHRH ethylamide in the induction of spawning in Cirrhinus mrigala and Labeo fimbriatus . Findings shows this LHRH analogue to be a promising substitute for the pituitary gland extract which is currently used. Further studies are required to standardize the dose and method of administration in the various cultivable species in India.

Captive breeding and embryonic development of honey gourami, Colisa sota (Ham.-Buch.)

Honey Gourami, Colisa sota, has high ornamental as well as food value. The natural resources of this species are gradually declining, due to destruction of its habitat, over fishing for aquarium trade and human consumption. The fish was bred in captivity under controlled environment. It laid about 200-400 eggs in bubble nest built by the male. Hatching started within 28-30hrs. after egg laying. The hatchlings became free swimming by 3rd to 4th day of hatching. The male showed territoriality and parental care by guarding the eggs and hatchlings. The larval survival was 30-35%. The breeding behavior, embryonic and post embryonic development of the fish were studied.

Spawning behaviour and induced breeding of an estuarine catfish, Mystus gulio (Ham.)

Spawning behaviour of hormone induced estuarine catfish, Mystus gulio was observed in captive condition. Spawning activities that include pairing, chasing and resting, nudging, and twisting, started about 5 hours post injection and ended with release of eggs within 1-2 hours of courtship. Three different dosages of "ovaprim" (1 ml/kg, 1.5 ml/kg, and 2 ml/kg in a single dose) were used in induced breeding of M gulio. The latency period was less (6-7 hours) with the dose of 1.5 and 2 ml/kg, while it was more (7-8 hours) with that of 1 ml/kg. However, all females spawned successfully with each of three different dosages, without any significant differences in the rate of fertilization and hatching. Eggs under all hormone dosages hatched between 18-20 hours after spawning. The hatching rate with 1, 1.5, and 2 ml/kg varied from 71.3-72.7%, corresponding to the fertilization rate of 80.7-84.7%.

Effects of rhFSH regimen and time interval on ovarian responses to repeated stimulation cycles in rhesus monkeys during a physiologic breeding season

We studied the effects of repeated stimulation by recombinant human FSH (rhFSH) at various time intervals during a physiologic breeding season in rhesus monkeys. Ovarian recovery and responses were assessed by ultrasonography, serum steroid concentrations

A comparative study on the induced breeding and in vitro fertilization performance by various inducing agents in catfish, Heteropneustes fossilis (Bloch)

Brood catfish, Heteropneustes fossilis were collected from Tamirabarani river basin of Tamil Nadu, India and kept in cement tanks. Three inducing hormones viz, Ovaprim, Ovatide and WOVA-FH were injected at the rate of 0.5, 1.0, 1.5 and 2.0 ml/kg body weight in order to induce oocyte maturation and ovulation. After 10-13h of injection at a water temperature of 27±-0.5°C, stripping of eggs and in vitro fertilization was done. Ovaprim gave maximum (94.67%) hatching rate followed by Ovatide (90.33%) and WOVA-FH (77.33%).

Preliminary success on hormone induced captive breeding of goldspot mullet, Liza parsia (Ham.)

An attempt was made to breed goldspot mullet, Liza parsia in captivity through hormone induction. The fish started spawning 35-36 hours after a single dose of 2ml ova prim per kg body weight. Hatching of fertilized eggs completed within 42-48 hours after spawning. The mean hatching rate (%) was 71.33±12 corresponding to the fertilization rate (%) of 64±12. The larvae started its first external feeding on the third day and attained a length 2.5±0.25 mm. The salinity of both breeding and rearing cisterns was 20‰ and temperature was maintained at 22-23°C.

Effects of dietary vitamin E on the growth and breeding performance of Ompok pabda

A 90 day feeding trail was conducted to investigate the effect of vitamin E on the growth and breeding performance of Ompok pabda. A total of 84 healthy female brood fish (41.10±0.44g) were divided into 4 treatments i.e. treatment T1 T2, T3 and T4 having three replications each. The fish were fed twice a day with a standard feed (40% protein) having 4 doses of vitamin E viz. 0 (served as control), 50, 100 and 150mg vitamin E/kg feed. At the end of the feeding trial, the brood fish were induced to breed with PG extract to observe the effect of vitamin Eon feed. After rearing for 90 days with the experimental feeds, it was found that weight gain and specific growth rate of brood fish fed with 100mg vitamin E/kg feed (treatment T3) was the highest (14.78±0.38g and 2.99±0.11) while 150mg vitamin E/kg feed (treatment T4) fed fish gave the poorest result (2.97±0.89g and 1.21±0.32). There was no significant difference in terms of length gain of brood fish among the different treatments. The brood fish were induced to breed with equal dose of PG extract (18 and 12mg PG/kg body weight for female and male respectively) to observe the dietary effect of vitamin E on breeding performance. The highest ovulation, fertilization and hatching rate of eggs were found to be 81.48±6.41%, 84.04±3.53% and 68.59±5.03% respectively in the brood fish of treatment T1 while the poorest (33.33±00%, 52.35±5.02% and 45.70±7.24% respectively) were found in the brood fish under treatment T4. The results suggest that inclusion of 100mg vitamin E/kg feed is best for enhancing the breeding performance of O. pabda brood fish indicating that vitamin E content has a positive impact on reproduction of fish. The present results also imply that inclusion of higher level of vitamin E exerts an antagonistic effect in terms of growth and breeding performance of this species.

Genetic study on breeding stock of the Indian mackerel along the Indian coast

The study included: sample collection; microsatellite genotyping and analysis; and preliminary results

Examining the possibilty of multiplication and breeding of (Schizothorav [sic] zarudnyi) in order to restoration of regional aquatics reservations of Hamoon Lake and taking its advantag [sic] for aquaculture

Schizothorax zarudnvi, is an endemic fish of east country waters. (Triple lagoons of Hamoon and relevant water resources) that in the world it is reported in this resource specially. This fish named Hamoon mahi is one of the most economically valuable species in this region. Because of the recent years droughts, Hamoon logoon has been drive since 2000. Also, semi-wells (a semi natural resource) were affected drastically by recent drought years and their volume reduced to nearly one third of their real volume and resulted in changing at growth and reproduction physiology process in Schizothorax zanidnyi, brood stocks. Beginning of this project was done from October 2003. It's field studies begun (brood catching) since November 2001 by two methods including entangling gairs and at semi wells of Sistan that (Beach seine) had maximum rate of preparing qualified brood stocks. Broods transferred to Cyprinidea reproduction work shop of Zahak and after taking primary measures they stored in to the edaphic pools. Increasing the success safety factor (coefficient) for artificial reproduction of Sthizothorax zarudnyi , identifying the appropriate tune for Hormonal acceptance (physiological preparation of broods) is needed , so this important work was done regularly by histological studies and GSI measurements since November. Highest GSI rates of females (%80.51) and highest IV stage abundance of sexual maturity (%l 00) were observed an march. On the base of this date, Hormone therapy was done on broods on march. The used hormones are as follows Hypophysis. extraction, GnRHa and Anti Dopamin at the dozes of 3-6 ml, 20-30kg and 10-15 ml per kg body weight respectively and 2-3 times from 11-12-80 they were injected. Injected broods kept in to two circumstances, flow-through (rounded pool) and stagnant systems. In stagnant system 14 and 19 individuals of female and male (Schizothorax zauiulnri) broods, respectively injected in 11th, 15111, 19th, and 24th of march 1380. Non of the injected broods in 11 and 15 and 19th march (in stagnant Condition) answered to Hormone therapy. After final injection broods had general less activity and a few of them died. Mean temperature of brood pond waters (daily) which were injected. Fluctuated between 10-25-13. 63°c but injected broods on 24th march had different characteristics. They had pale color and had few fecundity. In this stage of injection they hadn't any successful vulation. After injection, Mean daily water temperature was 15, 88-17, 54°c. In Flowing system, 13-16 individual of males and females respectively were injected on 15th, 19th, 22th and 23th march. None of injected producers on 15th and 19th march with mean daily water temperature of 10, 25-12°c were prepared for spawning but injected producers on 22nd an 23th march with mean daily water temperature of 13.5-1 rc responded about 75-100 percent. (Schizothorax zarudnyi) brood stocks were prepared for spawning after 353-428 hours/day from final injection. Diameter of obtained eggs (before fertilization) was between 1.9-2.3 min and of fertilized eggs was 3.8mm. Fertilized eggs of (Schizothorax zarudnyi) were hatched after 6-7 days with mean water temperature of 17.08°c. Mean length of on one day larvae was 9.47 mm. Larvae was 9.47 mm. Larvae adsorbed the whole yolk sac after , 5-6 days at 17- 1°c and were prepared for releasing in to edaphic pools. Because of the lack of necessary and complementary facilities in the region , they had to release them in to veniros and growing them for 8 days. At the end of 18th day , 35000 larvae (at first) released into an edaphic pond with a volume of 150m2. After growing them for one moth , mean length and weight of new hatched larvae was 29.41 mm and 1.12►r , respectively. With respect to results of this investigation , artificial reproduction of (Schizothorax zarudnyi) Can be possible at 14-17°C and flowing water with Hormonal treatment. It -s breeding has increased development than other cultural specious in the region. Due to high economical value of this specious in Sistan and ti-s specialization east waters of Iran and having high resistance and proper growth There is a need of it's development and reproduction and culture in fish culture fanns (edaphic ponds• two-purpose pools) at the region and country.