Toxicidade de Lantana camara (Verbenaceae) em operárias de Apis mellifera (Hymenoptera: Apidae)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Efeito da infestação do ácaro Varroa destructor (Anderson e Treuman, 2000) (Arachnida : Acari : Varroidae) no desenvolvimento de abelhas africanizadas Apis mellifera (Linnaeus, 1758) (Hymenoptera: Apidae)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Classification of honeys from Pará State (Amazon region, Brazil) produced by three different species of bees

Vinte e sete amostras de mel, produzidas em dez cidades do Estado do Pará (Região Amazônica, norte do Brasil) por três espécies diferentes de abelhas (Apis mellifera, Melipona fasciculata e Melipona flavoneata), foram analisadas em seus teores de elementos minerais (Al, As, Ba, Be, Bi, Ca, Cd, Co, Cr, Cu, Fe, K, Li, Mg, Mn, Na, Ni, Sr e Zn) e alguns parâmetros fisicoquímicos (cor, umidade, densidade, pH, sólidos insolúveis e solúveis totais, cinzas, condutividade elétrica, índice de formol, acidez livre, hidroximetilfurfural, açúcares redutores e totais e sacarose). Os teores minerais foram determinados via espectrometria de emissão atômica por plasma acoplado indutivamente (ICP OES) e as análises dos parâmetros físico-químicos seguiram metodologias oficiais. Os resultados das análises físico-químicas apresentaram-se de acordo com a legislação nacional e internacional, bem como com outros trabalhos similares ao redor do mundo. A análise estatística multivariada (análise por agrupamento hierárquico (HCA) e por componentes principais (PCA)) foi aplicada aos resultados dos teores metálicos e aos parâmetros físico-químicos, sendo possível a separação das amostras de mel conforme a espécie produtora.

Geoprópolis de Melipona fasciculata SMITH: ações citotóxica, imunomoduladora, antibacteriana e antifúngica

Pós-graduação em Patologia - FMB

Toxicidade de inseticidas para abelhas Apis mellifera L

Pós-graduação em Zootecnia - FMVZ

Efeito da cafeína no comportamento e na longevidade de operárias de abelhas africanizadas Apis mellifera L. (Hymenoptera: Apidae)

Pós-graduação em Ciências Biológicas (Zoologia) - IBRC

Efeito de recurso floral na composição qualitativa de apitoxina

Pós-graduação em Zootecnia - FMVZ

Influence of agrochemicals fipronil and imidacloprid on the learning behavior of Apis mellifera L. honeybees

Agrochemicals on crop cultivated areas is a source of contamination for bees and may cause physiological and behavioral disorders and mortality. The LD50 of the pesticides fipronil and imidacloprid was determined and their effect on the learning behavior of Apis mellifera L. honeybee evaluated. LD50 was determined by the ingestion of contaminated food with different concentrations of insecticide concentrations: Fipronil (0, 0.8, 0.4, 0.2, 0.1 and 0.05 µg bee-1) and imidacloprid (0, 0.4, 0.2, 0.1, 0.05 and 0.025 µg bee-1). The method of proboscis extension reflection (PER) and learning through citral odor evaluated their responses to food stimulation. LD50 obtained were 0.28 ± 0.11 and 0.10 ± 0.04 µg bee-1 for fipronil and imidacloprid, respectively. The PER test showed no significant difference (p < 0.05) although agrochemicals affected the learning of bees. Insecticides fipronil and imidacloprid are extremely harmful to foraging Africanized Apis mellifera bees.

Vasoconstrictor effect of Africanized honeybee (Apis mellifera L.) venom on rat aorta

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Honey bee lines selected for high propolis production also have superior hygienic behavior and increased honey and pollen stores

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Africanized honeybees in urban areas: a public health concern

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

FIPRONIL AND IMIDACLOPRID REDUCE HONEYBEE MITOCHONDRIAL ACTIVITY

Bees have a crucial role in pollination; therefore, it is important to determine the causes of their recent decline. Fipronil and imidacloprid are insecticides used worldwide to eliminate or control insect pests. Because they are broad-spectrum insecticides, they can also affect honeybees. Many researchers have studied the lethal and sublethal effects of these and other insecticides on honeybees, and some of these studies have demonstrated a correlation between the insecticides and colony collapse disorder in bees. The authors investigated the effects of fipronil and imidacloprid on the bioenergetic functioning of mitochondria isolated from the heads and thoraces of Africanized honeybees. Fipronil caused dose-dependent inhibition of adenosine 5'-diphosphate-stimulated (state 3) respiration in mitochondria energized by either pyruvate or succinate, albeit with different potentials, in thoracic mitochondria; inhibition was strongest when respiring with complex I substrate. Fipronil affected adenosine 5'-triphosphate (ATP) production in a dose-dependent manner in both tissues and substrates, though with different sensitivities. Imidacloprid also affected state-3 respiration in both the thorax and head, being more potent in head pyruvate-energized mitochondria; it also inhibited ATP production. Fipronil and imidacloprid had no effect on mitochondrial state-4 respiration. The authors concluded that fipronil and imidacloprid are inhibitors of mitochondrial bioenergetics, resulting in depleted ATP. This action can explain the toxicity of these compounds to honeybees. (c) 2014 SETAC

Modification of the brain proteome of Africanized honeybees (Apis mellifera) exposed to a subaEurolethal doses of the insecticide fipronil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Influence of Abiotic Factors and Floral Resource Availability on Daily Foraging Activity of Bees

In this study, the daily and seasonal influences of abiotic factors and the amount of floral resources on the foraging frequency of bees were determined. The experiments were performed, during 12 consecutive months, in the main floral sources used by bees in a secondary forest fragment. The foraging frequency of each bee species on flowers of each plant was recorded for 20-min periods, every hour. To verify whether the foraging activity is influenced by abiotic factors, Pearson's correlation analysis and linear regression tests were performed for the dominant bee species. Temperature and luminosity were the two main abiotic factors regulating foraging activities of bees. A positive correlation was found between the foraging frequency of most bees and these two variables. Conversely, the foraging activity was influenced neither by the relative humidity nor by the wind speed. The activity of each species depends on a combination of factors that include not only abiotic variables, but also the amount of floral resources available during the day, body size, and behavior of each visitor. After a certain period of the day, the scarcity of floral resources produced by most plants can stimulate the bees to forage in the flowers early in subsequent days, which may occur before the period in which the abiotic conditions are really favorable.

Hyperalgesic and edematogenic effects of Secapin-2, a peptide isolated from Africanized honeybee (Apis mellifera) venom

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)