First isolation of a rhabdovirus from perch Perca fluviatilis in Switzerland

Perca fluviatilis is a fish species of increasing interest to the Swiss fish farming industry. In recent years, recirculation systems have been specifically set up to increase production. In one of these farms, abnormal spiral swimming associated with elevated mortalities occurred in repeated batches of imported perch shortly after stocking on several occasions. No bacterial or parasitic etiology was detected, but a virus grown in bluegill fry (BF-2) cells was identified as perch rhabdovirus. Subsequent investigations of other samples suggested a viral tropism for the central nervous system (CNS). Phylogenetic analysis of the partial N and entire G gene sequences positioned this isolate in genogroup C of the species Perch rhabdovirus, with high nucleotide and amino acid (aa) sequence identities with the DK5533 strain isolated in Denmark in 1989. Comparative studies using other closely related isolates allowed the distinction of 2 serological Patterns among perch rhabdoviruses and the identification of a proline substitution by a serine in Position 147 of the glycoprotein potentially involved in antigenic differentiation. Even if perch imported onto the farm tested negative by virus isolation prior to transport, they may have been the origin of this outbreak since CNS tissue was not included in the samples that were analyzed. Another possibility might be a sub-clinical infection with a viral load in resident fish too low to be detected. This study reports the first isolation of a perch rhabdovirus in Switzerland, and emphasizes the necessity of optimizing diagnostic tools that facilitate better control of the risks associated with fish translocation.

Genetic stability of Schmallenberg virus in vivo during an epidemic, and in vitro, when passaged in the highly susceptible porcine SK-6 cell line

Schmallenberg virus (SBV), an arthropod-borne orthobunyavirus was first detected in 2011 in cattle suffering from diarrhea and fever. The most severe impact of an SBV infection is the induction of malformations in newborns and abortions. Between 2011 and 2013 SBV spread throughout Europe in an unprecedented epidemic wave. SBV contains a tripartite genome consisting of the three negative-sense RNA segments L, M, and S. The virus is usually isolated from clinical samples by inoculation of KC (insect) or BHK-21 (mammalian) cells. Several virus passages are required to allow adaptation of SBV to cells in vitro. In the present study, the porcine SK-6 cell line was used for isolation and passaging of SBV. SK-6 cells proved to be more sensitive to SBV infection and allowed to produce higher titers more rapidly as in BHK-21 cells after just one passage. No adaptation was required. In order to determine the in vivo genetic stability of SBV during an epidemic spread of the virus the nucleotide sequence of the genome from seven SBV field isolates collected in summer 2012 in Switzerland was determined and compared to other SBV sequences available in GenBank. A total of 101 mutations, mostly transitions randomly dispersed along the L and M segment were found when the Swiss isolates were compared to the first SBV isolated late 2011 in Germany. However, when these mutations were studied in detail, a previously described hypervariable region in the M segment was identified. The S segment was completely conserved among all sequenced SBV isolates. To assess the in vitro genetic stability of SBV, three isolates were passage 10 times in SK-6 cells and sequenced before and after passaging. Between two and five nt exchanges per genome were found. This low in vitro mutation rate further demonstrates the suitability of SK-6 cells for SBV propagation.

Magnetic resonance imaging and computer tomography of brain lesions in water buffaloes and cattle stunned with handguns or captive bolts

Owing to the demand for genuine mozzarella, some 330 water buffaloes are being slaughtered every year in Switzerland albeit a stunning procedure meeting animal welfare and occupational safety requirements remains to be established. To provide a basis for improvements, we sized anatomical specifics in water buffaloes and cattle and we assessed brain lesions after stunning with captive bolts or handguns by diagnostic imaging. In water buffaloes and cattle, the median distance from the frontal skin surface to the inner bone table was 74.0 mm (56.0–100.0 mm) vs 36.6 mm (29.3–44.3 mm) and from skin to the thalamus 144.8 mm (117.1–172.0 mm) vs 102.0 (101.0–121.0 mm), respectively. Consequently, customary captive bolt stunners may be inadequate. Free bullets are potentially suitable for stunning buffaloes but involve occupational safety hazards. The results of the present study shall be used to develop a device allowing effective and safe stunning of water buffaloes.

Na 1 Nachlass Max Horkheimer, 325 - Korrespondenzen u.a. mit Frances Bartlett Kinne und Otto Kirchheimer (p. V 101, 1-197)

75 Briefe mit Beilagen zwischen Prof. Frances Bartlett Kinne und Max Horkheimer, 1956-1973; 7 Briefe mit Beilagen zwischen Prof. Otto Kirchheimer und Max Horkheimer, 1952-1954; 19 Briefe mit Beilagen zwischen Prof. Guido Kisch und Max Horkheimer, 1955-1956; 23 Briefe mit Beilagen zwischen Walter Kittel (Oberbaurat i. R.), 1964-1973; 14 Briefe mit Beilagen zwischen Jules Klanfer und Max Horkheimer, 1963-1964; 4 Briefe mit Beilagen zwischen Prof. Otto Klineberg und Max Horkheimer, 1960-1961; 1 Brief von Max Horkheimer an Otto Klineberg (Psychologe), 22.6.1960;

Na 50 Nachlass Arthur Schopenhauer - 'Schopenhauer-Archiv', 101 - Dankesschreiben anlässlich der Zusendung eines Leuchters

u.a.: Ankündigung des Besuchs in Hamburg;

Na 50 Nachlass Arthur Schopenhauer - 'Schopenhauer-Archiv', 630 - Übersendung der Biographie

u.a.: Anmerkungen zu Georg Wilhelm Friedrich Hegel;

Ms. Praed. 101 - Metamorphoseon libri XV adiectis glossis marginalibus et interlinearibus

Vorbesitzer: Dominikanerkloster Frankfurt am Main

Ms. lat. qu. 101 - Capitulatio in libros XXIV epistolarum rerum familiarium

Vorbesitzer: Theodor Oehler; Karl Gottlieb Reinhard Oehler

Ms.Ff.F.Stoltze 3. 630 - Brief von Albert Varrentrapp an Friedrich Stoltze

Militärsache

Na 1 Nachlass Max Horkheimer, 630 - Typoskripte zur Vorlesung "Praktische Philosophie" und zu zwei Referaten (p. VIII 50a.2-VIII 51)

"Praktische Philosophie" (Zur Genealogie der Moral), Vorlesung Sommersemester 1962, Nachschrift (von Hilmar Tillack?): Nachschrift von 20 Vorlesungen 7.5.- 23.7.62, Durchschläge (unvollständig), Typoskript, 135 Blatt; N.N.: "Probleme der Geschichtsphilosophie bei Kant", Referat, Typoskript, 8 Blatt; N.N.: "Geschichtsphilosophie der Südwestdeutschen Schule", Referat, Typoskript mit handschriftlichen Randbemerkungen von Max Horkheimer, 18 Blatt;

Na 1 Nachlass Max Horkheimer, 665 - "Research Project on Anti-Semitism" (p. IX 101-IX 112)

Institute of Social Research; "Research Project on Anti-Semitism. General Statement of Scope of Project, Fields to be investigated, First Assignements, Plan of Operation, Joint Conferences" (15.3.1943), a) Typoskript, 5 Blatt, b) Typoskript, 3 Blatt, c) Typoskript mit handschriftlichen Ergänzungen, 5 Blatt; Institute of Social Research: "Notes on Some Methodological Principles and Some Tentative Assumptions for the Work of the Anti-Semitism Project" (15.3.1943), Typoskript, 3 Blatt; "Project on Anti-Semitism. Excerpt from a letter of D.R. (American Jewish Committee), dated 3/31/43" (31.03.1943), Typoskript mit handschriftlichen Ergänzungen, 2 Blatt; Institute of Social Research: "Research Project on Anti-Semitism. General Statement of Scope of Project, Fields to be investigated, First Assignements etc. Supplement: Los Angeles Group" (26.4.1943), a) Typoskript mit handschriftlichen Ergänzungen, 13 Blatt, b) Typoskript, 13 Blatt, c) Typoskript, 8 Blatt; Heinz Langerhans: "Methods to Evaluate the Attitude of Different Sections of the German Population toward Institutionalized Antisemitism" (6.5.1943), Typoskript mit eigenhändiger Korrektur, 5 Blatt; "Discussion with Messrs. George Mintzer and Newman Levy on present day U.S.A. Antisemitism" (5.3.1943), a) Typoskript, 4 Blatt b) Typoskript mit handschriftlichen Korrekturen, 4 Blatt; Re.: Antisemitism Project. Statement of Expense 15.03.1943 - 15.06.1943, Preliminary Budget 15.06.1943 - 15.03.1944, Aufstellungen für das American Jewish Committee, 15.06.1943, 4 Blatt; Paul Massing: "Some Notes to Fineberg's 'Overcoming Antisemitism'" (16.06.1943), Typoskript, 4 Blatt; "Luncheon with Mr. Hexter. Present: Dr. Pollock, Weil, Gurland, Massing and Mr. Hexter. Purpose: Discussion of S.A. Fineberg's book 'Overcoming Antisemitism'" (17.06.1943), Typoskript, 2 Blatt; "Luncheon with Mr. R.C. Rothschild in the Yale Club", Über Antisemitismus in den USA, 28.6.1943, Typoskript mit handschriftlichen Korrekturen, 1 Blatt; Friedrich Pollock: Memoranden über Besprechungen mit R.C. Rothschild, betreffs Anti-Semitism-Project (September 1943): 1. "Remarks of Mr. Rothschild concerning our Psychological Study in a meeting between him, P. and L." (21.9.1943); 2. "Memorandum on work's progress of Anti-Semitism-Project" (15.09.1943), Typoskript, 1 Blatt; 3. "Meeting R.C. Rothschild and Pollock", (15.09.1943), Typoskript, 2 Blatt; 4. "Memorandum No. 1" (14.09.1943), Typoskript, 1 Blatt; "Memorandum re: Antisemitism Project" (29.10.1943), Typoskript mit handschriftlichen Ergänzungen, 2 Blatt;

Ms.Ff.F.Stoltze 3. 101 - Brief von G. von Guaita an Laura Stoltze

Persönliche Nachrichten