Use of comet assay to assess DNA damage in patients infected by Helicobacter pylori - Comparisons between visual and image analyses

Studies of DNA damage in gastric epithelial cells of Helicobacter pylori (H. pylori)-infected patients are conflicting, possibly due to different methods used for scoring DNA damage by Comet assay. Therefore, we compared the sensitivity of visual microscopic analysis (arbitrary units-scores and comets%) and image analysis system (tail moment), in the gastric epithelial cells from the antrum and corpus of 122 H. pylori-infected and 32 non-infected patients. The feasibility of cryopreserved peripheral blood lymphocytes and whole-blood cells for DNA damage biomonitoring was also investigated. In the antrum, the levels of DNA damage were significantly higher in H. pylori-infected patients with gastritis than in non-infected patients with normal mucosa, when evaluated by image analysis system, arbitrary units and comets%. In the corpus, the comets% was not sufficiently sensitive to detect the difference between H. pylori-infected patients with gastritis and non-infected patients with normal mucosa. The image analysis system was sensitive enough to detect differences between non-infected patients and H. pylori-infected patients with mild gastritis and between infected patients with moderate and severe gastritis, in both antrum, and corpus, while arbitrary units and comets% were unable to detect these differences. In cryopreserved peripheral blood lymphocytes, the levels of DNA damage (tail moment) were significantly higher in H. pylori-infected patients with moderate and severe gastritis than in non-infected patients. Overall, our results indicate that the image analysis system is more sensitive and adequate to measure the levels of DNA damage in gastric epithelial cells than the other methods assayed. (c) 2005 Elsevier B.V. All rights reserved.

Purification, crystallization and preliminary X-ray analysis of haemoglobin I from the armoured catfish Liposarcus anisitsi

Liposarcus anisitsi is an armoured catfish that presents accessorial air oxygenation through a modified stomach, which allows this species to survive in waters with very low oxygen content. Analysis of its haemolysate has shown the presence of four haemoglobins; this work focuses on the main component, haemoglobin I. It has been crystallized in two different forms and X-ray diffraction data have been collected to 2.77 and 2.86 Angstrom resolution using synchrotron radiation. Crystals were determined to belong to the space groups C2 and P2(1) and preliminary structural analysis revealed the presence of one tetramer in the asymmetric unit in both crystal forms. The structure was determined using a standard molecular-replacement technique.

Uma contribuição da teoria literária para a análise de conteúdo de imagens publicitárias do fim do século XIX e primeira metade do século XX, contemplando aspectos da natureza brasileira

Pós-graduação em Ciência da Informação - FFC

Artes visuais e ensino médio: representações de professores sobre a prática pedagógica (2004-2010)

Este estudo constituiu-se numa análise das Representações de Professores de Artes Visuais acerca de suas práticas pedagógicas no contexto do Ensino Médio. Tendo como objetivo geral analisar as Representações de Professores de Artes Visuais do Ensino Médio sobre a Prática Pedagógica. A análise fora produzida a partir do conceito de Representações na perspectiva de Roger Chartier (1990; 1991;1994): sobre prática pedagógica com Veiga (2011; 2010): sobre formação de professores: Pimenta (2009) e Coelho (2009; 2010): em Arte com Barbosa (2009; 2010) e Fusari & Ferraz (2001; 2009). Utilizamos como caminho metodológico a Análise de Conteúdo de Bardin (2010). O estudo é uma pesquisa do tipo analítico- descritiva. Utilizamos como instrumento de coleta de dados: documentos oficiais e questionário, do qual participaram 15 professores de Arte efetivos da Rede Pública Estadual do Ensino Médio. Os resultados do estudo revelaram, que acerca da prática pedagógica, os professores, em sua maioria, apesar de terem um bom tempo de serviço, demonstraram a representação de uma triangulação apresentada em três vértices: leitura, baseada no senso comum, sem fundamentação Teórica/Ideal; o fazer, a prática no improviso, predominando o lado Prático/Real, e a contextualização superficial por falta de fundamentação, de unidade teórica/prática. Ressaltamos como aproximações conclusivas a vulnerabilidade na formação de professores de Arte. Essa formação é deficiente no que tange à capacidade de desenvolver a prática pedagógica com qualidade nas escolas públicas estaduais. Inferimos que urge a necessidade de (re) avaliar a formação de professores de Artes Visuais inicial e continuada, forjando um profissional com compromisso reflexivo-crítico com a sua prática social na educação.

O papel dos projetos sociais na vida de adolescentes em contextos potenciais de rico e processos de resiliência

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Influence of rootstocks and pruning times on yield and on nutrient content and extraction in 'Niagara Rosada' grapevine

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

A formação de professores para o trabalho com deficientes visuais: uma experiência inicial de colaboração a partir do desenvolvimento de materiais didáticos

Pós-graduação em Educação Escolar - FCLAR

A repercussão midiática das mensagens na camisa do corinthians nos sites esportivos Lance Net, Globo Esporte e ESPN

Pós-graduação em Comunicação - FAAC

Tecnologias virtuais e análise videográfica: o YouTube® como recurso de pesquisa para compreensão sobre a imagem do idoso brasileiro = Virtual technologies and videographic analysis : YouTube® as research resource for understanding about brazilian elderly imagem

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Etnobotânica, agroecologia e modelos alternativos em horticultura: análise de conteúdo da mídia impressa

Pós-graduação em Agronomia (Horticultura) - FCA

Etnobotânica, agroecologia e modelos alternativos em horticultura: análise de conteúdo da mídia impressa

Pós-graduação em Agronomia (Horticultura) - FCA

Análisis del proceso inclusivo del alumno ciego en clases de Física Moderna

This paper is aimed at understanding which the most important difficulties and alternatives to include students with visual impairments in physics classes. It presents and discusses the difficulties and viabilities of having a born blind student effectively attend Modern Physics classes. Using content analysis, this experiment identifies 6 functional classes that might correspond to difficulties and 4 which refer to the viabilities. Therefore, the importance of appropriate communicative environments, the including role of the interactivity element, as well as the need for destitution of segregation environments inside classroom, are emphasized.

Projeto gráfico de livros didáticos da educação de jovens e adultos: análise sob a perspectiva dos docentes de escolas de Bauru (SP) e de Avaré (SP)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Expressão gênica diferencial em tecido muscular de bovinos Nelore divergentes para qualidade de carne

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Institutional Processes and Discursive Strategies: Rhetoric and Vocabulary Analysis of CSR and Sustainability

The candidate tackled an important issue in contemporary management: the role of CSR and Sustainability. The research proposal focused on a longitudinal and inductive research, directed to specify the evolution of CSR and contribute to the new institutional theory, in particular institutional work framework, and to the relation between institutions and discourse analysis. The documental analysis covers all the evolution of CSR, focusing also on a number of important networks and associations. Some of the methodologies employed in the thesis have been employed as a consequence of data analysis, in a truly inductive research process. The thesis is composed by two section. The first section mainly describes the research process and the analyses results. The candidates employed several research methods: a longitudinal content analysis of documents, a vocabulary research with statistical metrics as cluster analysis and factor analysis, a rhetorical analysis of justifications. The second section puts in relation the analysis results with theoretical frameworks and contributions. The candidate confronted with several frameworks: Actor-Network-Theory, Institutional work and Boundary Work, Institutional Logic. Chapters are focused on different issues: a historical reconstruction of CSR; a reflection about symbolic adoption of recurrent labels; two case studies of Italian networks, in order to confront institutional and boundary works; a theoretical model of institutional change based on contradiction and institutional complexity; the application of the model to CSR and Sustainability, proposing Sustainability as a possible institutional logic.