Resistência de genótipos de tomateiro a Tuta absoluta (Meyrick, 1917) (Lepidoptera: Gelechiidae) e efeito no comportamento e desenvolvimento de podisus nigrispinus (Dallas, 1851) (Hemiptera: Pentatomidae)

Pós-graduação em Agronomia (Entomologia Agrícola) - FCAV

Ação de Bacillus thuringiensis Berliner nas características biológicas de outrs inimigos naturais

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Bioecologia e controle microbiano de sarsina violascens (Herrich-schaeffer, 1856) (Lepidoptera: Lymantriidae) em Eucalyptus spp

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Biologia e caracterização morfológica e morfometrica dos estágios imaturos e adulto de Calosoma granulatum Perty, 1830 (Coleoptera: Carabidae)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Caracterização do gene vip3A e toxicidade da proteína Vip3Aa50 à lagarta-do-cartucho e à lagarta-da-soja

O objetivo deste trabalho foi caracterizar o gene vip3A de Bacillus thuringiensis e verificar a toxicidade da proteína Vip3Aa50 a larvas da lagarta-do-cartucho (Spodoptera frugiperda) e da lagarta-da-soja (Anticarsia gemmatalis). O gene vip3A foi amplificado por PCR, com iniciadores específicos, e gerou um fragmento de 2.370 pb. Esse fragmento foi clonado em vetor pGEM-T Easy e, em seguida, sequenciado, subclonado em vetor de expressão pET-28a (+) e inserido em células de Escherichia coli BL21 (DE3). A expressão da proteína Vip3Aa50 foi induzida por isopropil-β-D-1-tiogalactopiranosídeo (IPTG), visualizada em SDS-PAGE e detectada por Western blot. Os ensaios de toxicidade revelaram alta atividade da proteína Vip3Aa50 contra as larvas neonatas da lagarta-da-soja e da lagarta-do-cartucho, com CL50 de 20,3 e 79,6 ng cm-2, respectivamente. O gene vip3Aa50 é um novo gene da classe vip3A.

Screening of Extracts of Leaves and Stems of Psychotria spp. (Rubiaceae) against Sitophilus zeamais (Coleoptera: Curculionidae) and Spodoptera frugiperda (Lepidoptera: Noctuidae) for Maize Protection

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Telenomus remus (Hymenoptera: Platygastridae) parasitism on eggs of Anticarsia gemmatalis (Lepidoptera: Eribidae) compared with its natural host Spodoptera frugiperda (Lepidoptera: Noctuidae)

The capacity of Telenomus remus to parasitize eggs of Anticarsia gemmatalis, compared with its natural host, Spodoptera frugiperda, was evaluated under different temperatures. The parasitoid T. remus was reared at 25 +/- 1 degrees C for a single generation on both hosts. After reaching the adult stage, they were allowed to parasitize both hosts to study parasitoid biology and parasitism capacity at temperatures between 19 degrees C and 37 +/- 1 degrees C. Egg-to-adult developmental time was similar on both hosts. The number of A. gemmatalis eggs parasitized was lower than that of S. frugiperda eggs at all temperatures. Parental female longevity of parasitoids was greater on A. gemmatalis eggs. This indicated a smaller metabolic expense during parasitism, a common feature observed on nonpreferable hosts. In general, sex ratio was little affected by temperature or hosts. When parental T. remus were reared on A. gemmatalis before the experiment, base temperature (Tb) and the thermal constant (K) were 9.53 degrees C and 209.57 DD on eggs of A. gemmatalis and 9.68 degrees C and 197.79 DD on eggs of S. frugiperda, respectively. When parental T. remus were reared on S. frugiperda eggs, Tb and K were 10.12 degrees C and 188.46 DD and 9.69 degrees C and 190.24 DD for the evaluated host eggs of A. gemmatalis and S. frugiperda, respectively. Therefore, T. remus develops in eggs of A. gemmatalis. This can be beneficial for its use in field crops where outbreaks of both Spodoptera spp. and A. gemmatalis occur. However, A. gemmatalis is a less favorable host for the parasitoid development.

Efeito do nível e da época de desfolha artificial sobre os componentes de produtividade de cultivares de algodoeiro

The periodic substitution of cotton cultivars for the farms can modify the levels of economic damages and control of pests, compromising the success in pest control. The objective of this work was to evaluate the effect of simulated defoliation levels in cotton cultivars actually used. The assay was carried out in experimental area of the Polo Apta Centro Norte, in Pindorama, Sao Paulo state. There were evaluated four defoliations levels (0%, 36.8%, 62.1% e 100%), done at a unique operation, in tree times (30, 60 and 90 days after plant emergence) and four cotton cultivars (IAC-25, DeltaOpal, Fibermax 966 and Fibermax 993). The defoliation was done with a scissor simulating the caterpillar damage. Evaluations included the number of open bolls, weight of one boll and 100 seeds, fiber percentage and productivity (kg ha(-1)). The defoliation negatively affected all components of agronomic traits, with the exception of percentage of fiber. The most damaging levels were 62.1 and 100% removal of the leaf blade. The period of higher susceptibility to defoliation occurs from 30 to 60 days after plant emergence.

Potencial predatório de Carabidae e Staphylinidae (Coleoptera) sobre a lagarta-da-soja

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Efeitos associados de genótipos de eucalipto sobre Thyrinteina arnobia (Stoll) (Lepidoptera: Geometridae) e no predador Podisus nigrispinus (Dallas) (Heteroptera: Pentatomidae)

Pós-graduação em Agronomia (Entomologia Agrícola) - FCAV

Suscetibilidade de Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) a entomopatógenos

Pós-graduação em Agronomia (Entomologia Agrícola) - FCAV

MORTALITY OF ANTICARSIA GEMMATALIS (LEPIDOPTERA: NOCTUIDAE) CATERPILLARS POST EXPOSURE TO A COMMERCIAL NEEM (AZADIRACHTA INDICA, MELIACEAE) OIL FORMULATION

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Determinação dos tipos de resistência de genótipos de soja ao ataque de Spodoptera cosmioides (Walker, 1858) (Lepdoptera: Noctuidae)

The aim of this work was to evaluate the types of resistance of soybean genotypes to the attack of Spodoptera cosmioides (Walker, 1858), under laboratory conditions. Plants of the soybean genotypes, IAC 100, Dowling, PI 227687, PI 274454, BR 16, IGRA RA 626 RR, PI 227682, BRSGO 8360, IGRA RA 516 RR e P 98Y11 RR, were cultivated in vases and placed in a greenhouse in order to have their leaves used afterwards in free-choice and no-choice no-preference for feeding tests and evaluation of the biological parameters of S. cosmioides fed on the genotypes. For free-choice no preference for feeding test, 10 leaf discs were prepared and placed in Petri dishes equidistantly among themselves, and then two recently-hatched caterpillar per genotype were release in the center of the plate. The same test was performed for third instar larvae, so that one caterpillar per genotype was used. In non-choice test, only one genotype was used in each Petri dish, where two recently-hatched or one third instar caterpillars were released, respectively. For both tests, the attractiveness was assessed at 1, 3, 5, 10, 15, 30, 60, 120, 360, 720 and 1440 minutes after the release of the insects. In addition, the leaf area consumed was evaluated. Randomized blocks design and completely randomized design were used for free-choice and non-choice tests, respectively, with 10 replications. In biology test, recently-hatched larvae were transferred to Petri dishes with leaves of the genotypes, evaluating the period of larvae, pupae and overall (larvae + pupae), viability of larvae, pupae and overall, weight of 12 days-old larvae and 24 hours-old pupae and longevity of the adults. Completely randomized design was used for this test, with 30 replications. Genotypes IGRA RA 516 RR and IGRA RA 626 RR were the least and the most consumed by recently-hatched larvae of S. cosmioides, respectively, in non-choice non-preference for feeding test. Genotypes PI 274454 and PI 227687 were...

Fatores e mecanismos que influenciam a resistência em soja a Anticarsia gemmatalis Hübner e Spodoptera frugiperda (J. E. Smith)

Pós-graduação em Agronomia (Entomologia Agrícola) - FCAV

Influência do volume reduzido e do estádio fenológico da planta de soja no controle da Anticarsia gemmatalis (Hübner, 1818)

Pós-graduação em Agronomia (Entomologia Agrícola) - FCAV