In vitro differentiation of murine hematopoietic progenitor cells toward the myeloid lineage occurs in response to Staphylococcus aureus and yeast species

We have studied the effect of inactivated microbial stimuli (Candida albicans, Candida glabrata, Saccharomyces boulardii, and Staphylococcus aureus) on the in vitro differentiation of lineage negative (Lin−) hematopoietic progenitor mouse cells. Purified Lin− progenitors were co-cultured for 7 days with the stimuli, and cell differentiation was determined by flow cytometry analysis. All the stimuli assayed caused differentiation toward the myeloid lineage. S. boulardii and particularly C. glabrata were the stimuli that induced in a minor extent differentiation of Lin− cells, as the major population of differentiated cells corresponded to monocytes, whereas C. albicans and S. aureus induced differentiation beyond monocytes: to monocyte-derived dendritic cells and macrophages, respectively. Interestingly, signaling through TLR2 by its pure ligand Pam3CSK4 directed differentiation of Lin− cells almost exclusively to macrophages. These data support the notion that hematopoiesis can be modulated in response to microbial stimuli in a pathogen-dependent manner, being determined by the pathogen-associated molecular patterns and the pattern-recognition receptors involved, in order to generate the populations of mature cells required to deal with the pathogen.

Morphometric redefinition of the component chronospecies of the Globorotalia conoidea - G. inflata lineage in DSDP Hole 29-284

Phylo-zonations (or lineage-zonations) are based upon morphological changes within individual evolutionary lineages. These zonations, although potentially of use for stratigraphic subdivision and correlation, often suffer from a lack of quantitative exactness in the definitions of chronospecies. Thus exact reproducibility is hindered for stratigraphic determinations. The potential of morphometrically defined phylo-zonations is demonstrated on a temperate South Pacific Late Cenozoic lineage of planktonic foraminifera (Globorotalia conoidea through intermediate forms to Globorotalia inflata in DSDP Site 284) exhibiting phyletic gradualism. Our sampling interval is about 0.1 m.y. during the last 8 m.y. Changes in the number of chambers in the final whorl, test conicalness, percentage of keeled forms, and test roundness or inflatedness, are used to quantitatively define the following five chronospecies: G. conoidea (Late Miocene; 6.1->8.3 m.y.), G. conomiozea (latest Miocene ; 5.3-6.1 m.y.), G. puncticulata sphericomiozea (earliest Pliocene; 4.5-5.3 m.y.), G. puncticulata puncticulata (Early-Middle Pliocene; 2.9-4.5 m.y.), and G. inflata (Late Pliocene-Quaternary; 0-2.9 m.y.). This phylo-zonation is directly applicable to temperate cool subtropical Southern Hemisphere areas where the evolution took place (Kennett, 1967, 1973; Scott, 1979). It is still not known if the lineage occurs elsewhere; thus the applicability of the phylo-zonation over broader areas is still uncertain. Trends in general size and aperture shape seem to be climatically controlled, and thus may be only of local stratigraphic utility. The practical applications of morphometric phylo-zonation for stratigraphy is to a large extent dependent upon the amount of time and effort required to statistically define the trends. Experiments with large numbers of subsamples from this lineage demonstrate that accurate stratigraphic determinations are possible from measurements on only 15 specimens per sample, except for those very close to chronospecies boundaries.

Quantitative study of evolution in the Sphaeroidinella lineage in mid-Pleistocene sediment of ODP Hole 154-926A (Appendix A)

Morphological evolution in the late Neogene planktonic foraminifer Sphaeroidinella lineage involves a sudden increase of the percentage of specimens equipped with supplementary apertures (from <30% to >70%) in the mid-Pliocene (about 3.5 Ma). This evolutionary transition, marked by the first occurrence of specimens with large supplementary apertures in the lineage, is denoted the Sphaeroidinella event. Changes in the proportions of the supplementary apertures in the lineage were studied in 24 samples from ODP Hole 926A drilled in the equatorial Atlantic Ocean. In addition, detailed chronological models have been compiled for this section as well as for Pliocene sections from DSDP Holes 214, 502A, and 503B, where evolution in the lineage have been analyzed previously. Stratigraphic correlation of the studied sequences suggests that the Sphaeroidinella event took place at about 3.6 Ma in the eastern equatorial Pacific (Hole 503B) and at 3.5-3.6 Ma in the Caribbean (Hole 502A), while in the Atlantic Ocean (Hole 926A) and in the Indian Ocean (Hole 214) the event occurred after 3.5 Ma. The inferred diachrony of the mid-Pliocene Sphaeroidinella transition, which is considered to represent a prime example of punctuated anagenesis, suggests that this evolutionary modality may have an allopatric component. Its short duration (on average less than 50 kyr) and the detailed biochronology that could be established for this event qualifies it as a useful biostratigraphic tool in the low-latitude Pliocene oceans.

Index of the Rolls of honor (ancestor's index) in the Lineage books of the National society of the Daughters of the American revolution, volumes 41 to 80.

Mode of access: Internet.

Eisenhower lineage and reference, 1691-September 3, 1957; a complete set of the series of bulletins on Eisenhower, Eisenhauer, Isenhour, Icenhower, Izanour, etc. family history which were issued between August 20, 1956 and December 31, 1957.

Mode of access: Internet.

The Army lineage book.

Includes bibliography.

Lineage book - National Society of the Daughters of the American Revolution.

Errata for the lineage book, v. 1-39, inclusive, 30 p.; v. 1-36, inclusive, 26 p. in v. 38; v. 1-27, inclusive, 20 p.; v. 1-21, inclusive, 10 p. in v. 21.

The lineage of Lichfield; an essay in eugenics ...

"This edition ... is limited to three hundred and sixty-five numbered copies, printed from type, each copy signed by the author. Copy no. 362."

Reed-Read lineage; Captain John Reed of Providence R.I. and Norwalk, Conn. and his descendants through his sons, John and Thomas, 1660-1909,

Mode of access: Internet.

Americans of royal descent. A collection of genealogies of American families whose lineage is traced to the legimate issue of kings.

Mode of access: Internet.

Index of the Rolls of honor (ancestor's index) in the Lineage books of the National society of the Daughters of the American revolution, volumes 1 to 160.

Vol. [2] has imprint: Washington, D.C., Press of Judd & Detweiler, inc., 1926.

John Walker Maury; his lineage and life. A sketch

Mode of access: Internet.

Lineage record of the members of the Society of Mayflower descendants in the state of Iowa, April, 1933 /

Mode of access: Internet.

Continued discovery of transcriptional units expressed in cells of the mouse mononuclear phagocyte lineage

The current RIKEN transcript set represents a significant proportion of the mouse transcriptome but transcripts expressed in the innate and acquired immune systems are poorly represented. In the present study we have assessed the complexity of the transcriptome expressed in mouse macrophages before and after treatment with lipopolysaccharide, a global regulator of macrophage gene expression, using existing RIKEN 19K arrays. By comparison to array profiles of other cells and tissues, we identify a large set of macrophage-enriched genes, many of which have obvious functions in endocytosis and phagocytosis. In addition, a significant number of LPS-inducible genes were identified. The data suggest that macrophages are a complex source of mRNA for transcriptome studies. To assess complexity and identify additional macrophage expressed genes, cDNA libraries were created from purified populations of macrophage and dendritic cells, a functionally related cell type. Sequence analysis revealed a high incidence of novel mRNAs within these cDNA libraries. These studies provide insights into the depths of transcriptional complexity still untapped amongst products of inducible genes, and identify macrophage and dendritic cell populations as a starting point for sampling the inducible mammalian transcriptome.

Morphological and physiological specialization for digging in amphisbaenians, an ancient lineage of fossorial vertebrates

Amphisbaenians are legless reptiles that differ significantly from other vertebrate lineages. Most species dig underground galleries of similar diameter to that of the animal. We studied the muscle physiology and morphological attributes of digging effort in the Brazilian amphisbaenid Leposternon microcephalum (Squamata; Amphisbaenia), which burrows by compressing soil against the upper wall of the tunnel by means of upward strokes of the head. The individuals tested (