Uma nova espécie de Crossodactylus da Serra do Cipó, Minas Gerais, Brasil (Amphibia, Leptodactylidae)

Crossodactylus bokermanni, sp. n., is described from Serra do Cipó, Minas Gerais, Southeastern Brasil. The new species is characterized by the small size (20-27 mm), slender build, long legs, narrow head, long and acuminate snout, rounded but fairly defined canthus rostralis, and small tympanum. Also, notes on its habits are made and its tadpole is described.

Morfo-anatomia de espécies de Blastocaulon Ruhland (Eriocaulaceae)

Estudou-se a morfo-anatomia das raízes, caules, escapos e folhas de Blastocaulon albidum, B. prostratum e B. scirpeum, que ocorrem nos campos rupestres de Minas Gerais, Brasil. Verificou-se que as espécies apresentam raízes muito finas com epiderme unisseriada, duas-três camadas de células do parênquima cortical e tecidos vasculares com poucos elementos. Os caules em B. albidum e B. prostratum são aéreos, prostrados, ramificados, enquanto em B. scirpeum alguns indivíduos apresentam rizoma ou caules muito curtos. Constituem-se de epiderme, córtex e cilindro vascular. As folhas são delicadas, com células da epiderme com paredes finas, parênquima clorofiliano frouxo e reduzido número de feixes vasculares colaterais. Os escapos são cilíndricos, finos, não formam costelas e possuem apenas quatro feixes vasculares colaterais. As características observadas nessas espécies estudadas são típicas de plantas que ocorrem em locais úmidos e sombreados, ambientes onde é encontrada a maioria das espécies de Blastocaulon e ainda caracterizam anatomicamente o gênero, que se diferencia de Paepalanthus.

Two new genera of neotropical Chrysopini (Neuroptera : Chrysopidae)

Two new genera and two new spec ies of Chrysopini (Neuroptera: Chrysopidae) are described from Brazil. The main characteristics of Cryptochrysa chloros are: a sheath of long setae in the oral cavity; slender forewing; triangular intramedian cell; antennal scape longer than wide; and lines of strong setae ventral to the arcessus. Characteristics of Furcochrysa allata are: large gonocornua; presence of a pseudopenis; arcessus absent; and basal marginal vein of forewing forked.

Mutagenic activity of sweepings and pigments from a household-wax factory assayed with Salmonella typhimurium

The mutagenic activity of garbage originating from a household wax industry was determined by the Salmonella/microsome assay, using the bacterial strains TA100, TA98 and YG1024. The garbage was obtained by sweeping the floor of the factory at the end of the work shift. Organic compounds were extracted by ultrasound for 30 min in dichloromethane or 70% ethanol. After evaporation of solvent, these extracts (HFS: household-wax factory sweepings) were dissolved in DMSO, and were tested for the mutagenic activity at varying concentrations (HFS-ET: 0.08-0.68 mg/plate, HFS-DCM: 0.60-7.31 mg/plate). The colouring agents (pigments) used in the production of the wax were also dissolved in DMSO and tested with the assay. The concentrations tested for each pigment were: Amaranth: 0.46-3.65 mg/plate, Auramine: 0.15-1.2 mg/plate, Tartrazine: 0.46-3.65 mg/plate and Rhodamine B: 0.22-1.82 mg/plate. Both ET and DCM organic extracts had mutagenic activity, especially in the YG1024 strain. The pigments behaved in a similar way, demonstrating that YG1024 was the most sensitive strain for the detection of mutagenicity, and that metabolization increased the activity. Human exposure (occupational and non-occupational) to industrial residues generated during the household-wax manufacturing and packaging process should be monitored, since this type of garbage is normally deposited in the environment without any control. (C) 2004 Elsevier Ltd. All rights reserved.

4 SPECIES OF BRAZILIAN POISON FROGS RELATED TO EPIPEDOBATES-PICTUS (DENDROBATIDAE) - TAXONOMY AND NATURAL-HISTORY OBSERVATIONS

We present morphological and natural history data on Epipedobates pictus and three additional taxa currently regarded as synonyms of the former: Epipedobates braccatus, E. flavopictus, and E. hahneli. Topotypes of the four species were examined. Morphological data on tadpoles and adults, in addition to distribution patterns and vocalizations, indicate that the four taxa represent distinct species. Two groups are evident within them: (1) the slender-bodied, Amazonian E. hahneli with small flash marks on the thighs and a low-finned tadpole, and (2) the robust bodied, almost extra-Amazonian E. braccatus, E. flavopictus, and E. pictus with large flash marks on the thighs and tadpoles with fins of moderate height.

A NEW SPECIES of THE BOKERMANNOHYLA MARTINSI GROUP FROM CENTRAL BAHIA, BRAZIL WITH COMMENTS on BOKERMANNOHYLA (ANURA: HYLIDAE)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

ESTABILIDADE TERMICA DO PARACETAMOL EM SOLUCAO: CROMATOGRAFIA EM CAMADA DELGADA (CCD) - ESPECTROFOTOMETRIA ULTRAVIOLETA

The interference practised by the products in degradation of paracetemol when there is the application of spectrophotometry UV is the main obstruction to the execution of studies of thermic stability. The application of chromatography in slender layer to the isolation of paracetamol, besides being the excessively hard to apply was satisfactory to the desired proposal. The type and extension of degradation suffered by paracetamol in solution suggest the convenient inclusion, in the formulations, of one system antioxidant. This practice makes possible the blockage of the oxidation of p-aminophenol, produced by the hydrolytic degradation of paracetamol; this fact propitiated the diminution of the number of products of degradation in the medicine, making the use more secure. On the other hand, considering especially the methodological necessities of the present work, the presence of one antioxidant system facilitated the separation of paracetamol through the Thin Layer Chromatography and consequently optimized its quantification by Spectrophotometry UV during the study of thermic stability. The formulation proposed revealed excellent stability.

Effect of organic synthetic food colours on mitochondrial respiration

Eleven organic synthetic dyes, currently or formerly used as food colours in Brazil, were tested to determine their effect on mitochondrial respiration in mitochondria isolated from rat liver and kidney. The compounds tested were: Erythrosine, Ponceau 4R, Allura Red, Sunset yellow, Tartrazine, Amaranth, Brilliant Blue, Indigotine Blue, Fast Red E, Orange GGN and Scarlet GN. All food colours tested inhibited mitochondrial respiration (State III respiration, uncoupled) supported either by α-ketoglutarate or succinate. this inhibition varied largely, e.g. from 100% to 16% for Erythrosine and Tartrazine respectively, at a concentration of 0.1 mg food colour per mitochondrial protein. Both rat liver and kidney mitochondria showed similar patterns of inhibition among the food colours tested. This effect was dose related and the concentration to give 50% inhibition was determined for some of the dyes. The xanthene dye Erythrosine, which showed the strongest effect, was selected for further investigation on mitochondria in vivo.

Regression of the lateral oviducts during the larval-adult transformation of the reproductive system of Melipona quadrifasciata and Frieseomelitta varia

Toward the end of the larval phase (pre-pupa), the reproductive systems of Melipona quadrifasciata and Frieseomelitta varia workers are anatomically similar. Scanning electron microscopy showed that during this developmental phase the right and left ovaries are fused and form a heart-shaped structure located above the midgut. Each ovary is connected to the genital chamber by a long and slender lateral oviduct. During pupal development, the lateral oviducts of workers from both species become extremely reduced due to a drastic process of cell death, as shown by transmission electron microscopy. During the lateral oviduct shortening, their simple columnar epithelial cells show some signs of apoptosis in addition to necrosis. Cell death was characterized by cytoplasmic vesiculation, peculiar accumulation of glycogen, and dilation of cytoplasmic organelles such as mitochondria and rough endoplasmic reticulum. The nuclei, at first irregularly contoured, became swollen, with chromatin flocculation and various areas of condensed chromatin next to the nuclear envelope. At the end of the pupal phase, deep recesses marked the nuclei. At emergence, worker and queen reproductive systems showed marked differences, although reduction in the lateral oviducts was an event occurring in both castes. However, in queens the ovarioles increased in length and the spermatheca was larger than that of workers. At the external anatomical level, the reproductive system of workers and queens could be distinguished in the white- and pink-eyed pupal phase. The metamorphic function of the death of lateral oviduct cells, with consequent oviduct shortening, is discussed in terms of the anatomical reorganization of the reproductive system and of the ventrolateral positioning of adult worker bee ovaries. (C) 2000 Wiley-Liss, Inc.

Distribution and abundance of four caiman species (Crocodylia: Alligatoridae) in Jaú National Park, Amazonas, Brazil

Jaú National Park is a large rain forest reserve that contains small populations of four caiman species. We sampled crocodilian populations during 30 surveys over a period of four years in five study areas. We found the mean abundance of caiman species to be very low (1.0 ± 0.5 caiman/km of shoreline), independent of habitat type (river, stream or lake) and season. While abundance was almost equal, the species' composition varied in different waterbody and study areas. We analysed the structure similarity of this assemblage. Lake and river habitats were the most similar habitats, and inhabited by at least two species, mainly Caiman crocodilus and Melanosuchus niger. However, those species can also inhabit streams. Streams were the most dissimilar habitats studied and also had two other species: Paleosuchus trigonalus and P. palpebrosus. The structure of these assemblage does not suggest a pattern of species associated and separated by habitat. Trends in species relationships had a negative correlation with species of similar size, C. crocodilus and P. trigonatus, and an apparent complete exclusion of M. niger and P. trigonatus. Microhabitat analysis suggests a slender habitat partitioning: P. trigonatus was absent from river and lake lgapo (flooded forest), but frequent in stream Igapó. This species was the most terrestrial and found in microhabitats similar to C crocodilus (shallow waters, slow current). Melanosuchus niger inhabits deep, fast moving waters in different study areas Despite inhabiting the same waterbodies in many surveys, M. niger and C. crocodilus did not share the same microhabitats. Paleosuchus palpebrosus was observed only in running waters and never in stagnant lake habitats. Cluster analysis revealed three survey groups: two constitute a mosaic in floodplains. (a) a cluster with both M. niger and C crocodilus, and another (b) with only C. crocodilus. A third cluster (c) included more species, and the presence of Paleosuchus species. There was no significant difference among wariness of caimans between disturbed and undisturbed localities. However, there was a clear trend to increase wariness during the course of consecutive surveys at four localities, suggesting that we, more than local inhabitants, had disturbed caimans. The factors that are limiting caiman populations can be independent of human exploitation. Currently in Amazonia, increased the pressure of hunting, habitat loss and habitat alteration, and there is no evidence of widespread recovery of caiman populations. In large reserves as Jaú without many disturbance, most caiman populations can be low density, suggesting that in blackwater environments their recovery from exploitation should be very slow.

Encapsulação da rifampicina em matrizes poliméricas flutuantes obtidas por liofilização a partir de metilcelulose e ftalato de hidroxipropilmetilcelulose. Avaliação da liberação in vitro

Floating multiparticles for oral administration with different compositions were studied from a matricial polymeric system to obtain sustained release. The polymers used in the multiparticles constitution were methylceullose (MC) and hydroxypropylmethylcelullose phthalate (HPMCP) in several proportions. Spherical and isolated structures were obtained using HPMCP/MC in the range from 1:3 to 1: 13. The diameters of the floating multiparticles were in the range from 3 to 3.25 mm, while the non-floating particles were between 1.75 and 2.1 mm. The morphological analysis by confocal microscopy showed that the probable mechanism of drug release was the diffusion from the inner of particles to external media. The encapsulation of hydrophilic model substances (tartrazin and bordeaux S), showed that the maximum incorporation was about 38%, while for the lipophilic model substances (rifampicin) was 45%. The in vitro release of rifampicin in acid medium was dependent on the ratio HPMCP/MC. In alkaline medium the release followed a two-step profile, with slow release in the initial times and subsequent increase in the higher times The initial drug delivery profile was not dependent on the MC/HPMCP ratio and can be related with the release of the antibiotic from multiparticle inner caused by the swelling of polymers by the presence of water in the system. However, afterwards the release proceeds with typical profile of process involving hydrogels systems.

A contribution for nonlinear structural dynamics characterization of cantilever beams

Successful experiments in nonlinear vibrations have been carried out with cantilever beams under harmonic base excitation. A flexible slender cantilever has been chosen as a convenient structure to exhibit modal interactions, subharmonic, superharmonic and chaotic motions, and others interesting nonlinear phenomena. The tools employed to analyze the dynamics of the beam generally include frequency- and force-response curves. To produce force-response curves, one keeps the excitation frequency constant and slowly varies the excitation amplitude, on the other hand, to produce frequency-response curves, one keeps the excitation amplitude fixed and slowly varies the excitation frequency. However, keeping the excitation amplitude constant while varying the excitation frequency is a difficult task with an open-loop measurement system. In this paper, it is proposed a closed-loop monitor vibration system available with the electromagnetic shaker in order to keep the harmonic base excitation amplitude constant. This experimental setup constitutes a significant improvement to produce frequency-response curves and the advantages of this setup are evaluated in a case study. The beam is excited with a periodic base motion transverse to the axis of the beam near the third natural frequency. Modal interactions and two-period quasi-periodic motion are observed involving the first and the third modes. Frequency-response curves, phase space and Poincaré map are used to characterize the dynamics of the beam.

Um surto de coccidiose em perdizes (Rhynchotus rufescens), criadas em cativeiro, por Eimeria rhynchoti Reis e Nóbrega, 1936 (Apicomplexa: Eimeriidae).

Eimeria rhynchoti is redescribed parasitizing partridge (Rhynchotus rufescens), reared in captivity, from Jaboticabal City, São Paulo State, Brazil. Sporulation takes place in 48 hours, the shape of oocysts found vary from spherical to elliptic with 23.01 micro +/- 1.57 of length by 21.0 micro +/- 1.78 of width. The microple, polar cap and residuum of the oocysts were absent. The oocyst wall, measures 2.2 micro +/- 0.31 of thickness, is composed by two smooth layers; the polar granule is present. The sporocysts length was 15.03 mm +/- 2.12 by 8.08 mm +/- 0.84 of width vary from elliptic to elongate. Sporocyst wall slender with is fine and Stieda body; the residue found in form of several smaller granules spherical compacts. The sporozoites are contrary extending along the sporocysts wall possessing refracts body of easy visualization.

Morphological adaptations to arboreal habitats and heart position in species of the neotropical whipsnakes genus Chironius

The evolution of arboreality in snakes is accompanied by modifications that are remarkably similar across species. Gravity is one of the most important selective agents, and arboreal snakes present adaptations to circumvent the gradient of pressure, including modifications on heart position (HP) and body slenderness (BS). However, the degree to which different life-history traits influence the cardiovascular system of snakes remains unclear. Here, we used an ecological and a phylogenetic approach to explore the relationship between habitat, HP, BS, and heart size (HS) in five species of the neotropical whipsnakes genus Chironius that occupy terrestrial, semiarboreal, and arboreal habits. Our ecological comparison indicated that the arboreal species have the most posterior-positioned heart, the most slender body, and the smallest HS, whereas the terrestrial representative of the group exhibited the most anterior heart, the less flattened body, and the largest HS. After removing the phylogenetic effect, we found no difference in HP and BS between terrestrial and arboreal species. Habitat only differed when contrasting with HS. Body slenderness and HS were correlated with HP. Our results suggest that different restrictions, such as anatomical constraints, behavior, and phylogenetic inertia, may be important for the studied species. © 2013 The Royal Swedish Academy of Sciences.

História natural das serpentes dos gêneros Echinanthera e Taeniophallus (Echinantherini)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)