Comonitoring of adenosine and dopamine using the wireless instantaneous neurotransmitter concentration system : proof of principle : laboratory investigation

Object

The authors of previous studies have demonstrated that local adenosine efflux may contribute to the therapeutic mechanism of action of thalamic deep brain stimulation (DBS) for essential tremor. Real-time monitoring of the neurochemical output of DBS-targeted regions may thus advance functional neurosurgical procedures by identifying candidate neurotransmitters and neuromodulators involved in the physiological effects of DBS. This would in turn permit the development of a method of chemically guided placement of DBS electrodes in vivo. Designed in compliance with FDA-recognized standards for medical electrical device safety, the authors report on the utility of the Wireless Instantaneous Neurotransmitter Concentration System (WINCS) for real-time comonitoring of electrical stimulation–evoked adenosine and dopamine efflux in vivo, utilizing fast-scan cyclic voltammetry (FSCV) at a polyacrylonitrile-based (T-650) carbon fiber microelectrode (CFM).
Methods

The WINCS was used for FSCV, which consisted of a triangle wave scanned between −0.4 and +1.5 V at a rate of 400 V/second and applied at 10 Hz. All voltages applied to the CFM were with respect to an Ag/AgCl reference electrode. The CFM was constructed by aspirating a single T-650 carbon fiber (r = 2.5 μm) into a glass capillary and pulling to a microscopic tip using a pipette puller. The exposed carbon fiber (the sensing region) extended beyond the glass insulation by ~ 50 μm. Proof of principle tests included in vitro measurements of adenosine and dopamine, as well as in vivo measurements in urethane-anesthetized rats by monitoring adenosine and dopamine efflux in the dorsomedial caudate putamen evoked by high-frequency electrical stimulation of the ventral tegmental area and substantia nigra.
Results

The WINCS provided reliable, high-fidelity measurements of adenosine efflux. Peak oxidative currents appeared at +1.5 V and at +1.0 V for adenosine, separate from the peak oxidative current at +0.6 V for dopamine. The WINCS detected subsecond adenosine and dopamine efflux in the caudate putamen at an implanted CFM during high-frequency stimulation of the ventral tegmental area and substantia nigra. Both in vitro and in vivo testing demonstrated that WINCS can detect adenosine in the presence of other easily oxidizable neurochemicals such as dopamine comparable to the detection abilities of a conventional hardwired electrochemical system for FSCV.
Conclusions

Altogether, these results demonstrate that WINCS is well suited for wireless monitoring of high-frequency stimulation-evoked changes in brain extracellular concentrations of adenosine. Clinical applications of selective adenosine measurements may prove important to the future development of DBS technology.

A proof of concept for using brief Dialectical Behavior Therapy as a treatment for problem gambling

Fourteen ‘treatment resistant’ problem gamblers received 9 weeks of Dialectical Behavior Therapy (DBT) at specialist problem gambling services delivered in Melbourne, Australia. This study is the first to investigate the effectiveness of a brief DBT treatment for problem gambling, with a focus on measuring change in the four DBT process skills (mindfulness, distress tolerance, emotion dysregulation, and negative relationships). Although there were no statistically significant improvements in measures of gambling behaviour, 83% of participants were abstinent or reduced their gambling expenditure pre- to post-treatment. Participants also reported statistically and clinically significant improvements in psychological distress,  mindfulness, and distress tolerance. Moreover, there were no increases in alcohol or substance use. These results are discussed in the context of focusing on a single DBT process skill, and the benefits of using group-based approaches.

Representative actions, proof of fact and author identification in copyright law: a history and a concern

Recent judgments in Australia have called for author identification in order that copyright subsistence may be established. There is a risk that such calls will be taken too literally, to the detriment of author privacy. This article considers the legal mechanisms by which author identity has historically been shielded from disclosure, without the operation of the copyright system being impaired. It expresses the hope that those who are responsible for developing copyright law will be mindful of the concern for author privacy which has long been part of copyright discourse.

Offline grouping proof protocol for RFID systems

Several grouping proof protocols have been proposed over the years but they are either found to be vulnerable to certain attacks or do not comply with EPC Class-1 Gen-2 (C1G2) standard because they use hash functions or other complex encryption schemes. Also, synchronization of keys, forward security, proving simultaneity, creating dependence, detecting illegitimate tags, eliminating unwanted tag processing and denial-of-proof (DoP) attacks have not been fully addressed by many. Our protocol addresses these important gaps and is based on Quadratic Residues property where the tags are only required to use XOR, 128-bit Pseudo Random Number Generators (PRNG) and Modulo (MOD) operations which can be easily implemented on low-cost passive tags and hence achieves EPC C1G2 compliance.

RFID tags : grouping proof with forward security

Several grouping proof protocols have been proposed over the years but they are either found to be vulnerable to certain attacks or do not comply with EPC Class-1 Gen-2 (C1G2) standard because they use hash functions or other complex encryption schemes. Among other requirements, synchronization of keys, forward security, dependence, detecting illegitimate tags, eliminating unwanted tag processing and denial-of-proof (DoP) attacks have not been fully addressed by many. Our protocol addresses these important gaps and is based on simple XOR encryption and 128-bit Pseudo Random Number Generators (PRNG), operations that are easily implemented on low-cost passive tags and hence achieves EPC C1G2 compliance.

Environmental invitingness for transport-related cycling in middle-aged adults: a proof of concept study using photographs

Introduction: Current evidence on associations between modifiable environmental characteristics and transport-related cycling remains inconsistent. Most studies on these associations used questionnaires to determine environmental perceptions, but such tools may be subject to bias due to unreliable recall. Moreover, questionnaires only measure separate environmental characteristics, while real environments are a combination of different characteristics. To overcome these limitations, the present proof of concept study used panoramic photographs of cycling environments to capture direct responses to the physical environment. We examined which depicted environmental characteristics were associated to environments' invitingness for transportation cycling. Furthermore, interactions with gender and participants' cycling behavior were examined. Methods: Fifty-nine middle-aged adults were recruited through purposeful convenience sampling. During a home visit, participants took part in a structured interview assessing demographics and PA during the preceding seven days, followed by an intuitive choice task and a (cognitive) rating task, which both measured 40 photographed environments' invitingness to cycle along. Multi-level cross-classified analyses were conducted using MLwiN 2.26. Results: Both tasks' multivariate results showed that presence of vegetation was identified as the most important environmental characteristic to invite people for engaging in transportation cycling, even when the amount of vegetation was relatively small. In the bivariate analyzes, some differences between results of the cognitive rating task and the intuitive choice task were found, showing that invitingness measured by the rating task was associated with environmental maintenance and cycling infrastructure, whereas invitingness determined by the choice task was associated with more traffic-oriented characteristics. Moreover, only for the choice task's results, moderating effects of gender and participants' cycling behavior in the preceding week were observed. Conclusion: The present study provides proof of concept that capturing people's less cognitive, more intuitive responses to an environment's invitingness for transport-related cycling may be important for revealing environment-behavior associations. If replicated in future studies using larger samples, results of our innovative measurements with photographs, especially those on vegetation, can complete the existing knowledge on which environmental characteristics are important for transportation cycling in adults and could form a basis to inform health promoters and local policy makers. However, future studies replicating our study method in larger samples and other population subgroups are highly encouraged. Moreover, causal relationships should be explored.

A robust grouping proof protocol for RFID EPC C1G2 tags

Several grouping proof protocols for RFID systems have been proposed over the years but they are either found to be vulnerable to certain attacks or do not comply with the EPC class-1 gen-2 (C1G2) standard because they use hash functions or other complex encryption schemes. Among other requirements, synchronization of keys, simultaneity, dependence, detecting illegitimate tags, eliminating unwanted tag processing, and denial-of-proof attacks have not been fully addressed by many. Our protocol addresses these important gaps by taking a holistic approach to grouping proofs and provides forward security, which is an open research issue. The protocol is based on simple (XOR) encryption and 128-bit pseudorandom number generators, operations that can be easily implemented on low-cost passive tags. Thus, our protocol enables large-scale implementations and achieves EPC C1G2 compliance while meeting the security requirements.

Zero knowledge grouping proof protocol for RFID EPC C1G2 Tags

In this paper, we propose a novel zero knowledge grouping proof protocol for RFID Systems. Over the years, several protocols have been proposed in this area but they are either found to be vulnerable to certain attacks or do not comply with the EPC Class 1 Gen 2 (C1G2) standard because they use hash functions or other complex encryption schemes. Also, the unique design requirements of grouping proofs have not been fully addressed by many. Our protocol addresses these important security and design gaps in grouping proofs. We present a novel approach based on pseudo random squares and quadratic residuosity to realize a zero knowledge system. Tag operations are limited to functions such as modulo (MOD), exclusive-or (XOR) and 128 bit Pseudo Random Number Generators (PRNG). These can be easily implemented on passive tags and hence achieves compliance with the EPC Global standard while meeting the security requirements.