963 resultados para nevo do tecido conjuntivo


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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Dermatosparaxis in animals is an autosomal recessive disorder of the connective-tissue clinically characterized by skin fragility and hiperextensibility. The disease in White Dorper sheep is caused by mutation (c.421G>T) in the ADAM metalloproteinase with thrombospondin type 1 motif, 2 (ADAMTS2) gene. This study describes the dermatological, histological and the molecular findings of the dermatosparaxis in White Dorper sheep from a herd located in the center-west of São Paulo State. The herd consisted of one ram, four ewe and their lambs. In this herd two lambs had clinical signs consistent with dermatosparaxis. Histopathological evaluation of the affected skin of these two animals also revealed consistent findings with dermatosparaxis, characterized by dysplasia of the collagen, which were arranged in small and fragmented collagen bundles and with foci of degeneration of collagen. Prominent cutaneous appendages and severe hemorrhagic focus in dermis region associated with mild neutrophilic infiltrate in the deep dermis. PCR using DNA blood and specific primers to amplify the mutation region c.421G>T was optimized in order to perform molecular diagnosis of the disease. The direct sequencing of the PCR products proved that the two clinically affected animals had the mutation responsible for dermatosparaxis, previously described for this breed and allowed the definitive diagnosis of the disease. This is the first report of the dermatosparaxis in White Dorper sheep in Brazil and the methodology used to confirm the diagnosis could be used in future studies to assess the prevalence of this mutation in Brazil, allowing the adoption of measures to prevent the spread of this mutation in the Brazilian White Dorper herd.

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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The aim of this study was to evaluate the influence of the bed in High Density Porous Polyethylene (Medpor) implants integration. Two different kind of beds -- corticalized and decorticalized -- were made in six dogs. The animals were killed thirty, sixty and ninety days after implantation. Blocks including implant and adjacent bone were harvested, submitted to histological procedures, stained in hematoxiline-eosine and analyzed in optic microscope. The results demonstrated an absence of infection and foreign body reaction associated with High Density Porous Polyethylene (Medpor) implants. Furthermore, bony and fibrovascular ingrowth could be observed into its pores. The best integration was noted in decorticalized group, in which occurred a greater bony and fibrovascular ingrowth in comparison to the corticalized group

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)