The effect of a mono component protease and different AMEn levels in broiler diets on growth performance from 1 to 18 days of age.

The objective of the present study was to evaluate the effect of the inclusion of a mono component serine protease (RONOZYME ProAct, DSM Nutritional Products) in diets with two different AMEn contents on apparent ileal digestibility (AID) of amino acids (AA) and growth performance in broilers from 1 to 18 days of age.

[Mono con espejo] [Material gr��fico]

Resumen: Descripci��n: representaci��n aleg��rica de la vanidad en la figura de un mono sujetando un espejo

Furs, capitols, prouisions e actes de Cort, fets y atorgats per ... don Phelip ... en les Corts generals per aquell celebrades als regnicols de la Ciutat y Regne de Valencia, en la vila de Mon��o, en lo Any M.DC.XXVI [Texto impreso]

Colof��n

Furs, capitols, prouisions e actes de cort fets per... Don Phelip princep e primogenit... del emperador y rey ... e Gobernador general dels regnes de la Corona de Arago [Texto impreso] : en les corts generals per aquell celebrades als regnicols de la ciutat y regne de Valencia, en la vila de Mon��o, en lo any MDXXXXVII

Hay un ejemplar encuadernado con: Furs, capitols, prouisions e actes de Cort (XVI/6).

Enhanced green fluorescence by the expression of an Aequorea victoria green fluorescent protein mutant in mono- and dicotyledonous plant cells.

The expression of the jellyfish green fluorescent protein (GFP) in plants was analyzed by transient expression in protoplasts from Nicotiana tabacum, Arabidopsis thaliana, Hordeum vulgare, and Zea mays. Expression of GFP was only observed with a mutated cDNA, from which a recently described cryptic splice site had been removed. However, detectable levels of green fluorescence were only emitted from a small number of protoplasts. Therefore, other mutations in the GFP cDNA leading to single-amino acid exchanges in the chromophore region, which had been previously studied in Escherichia coli, were tested in order to improve the sensitivity of this marker protein. Of the mutations tested so far, the exchange of GFP amino acid tyrosine 66 to histidine (Y66H) led to detection of blue fluorescence in plant protoplasts, while the exchange of amino acid serine 65 to cysteine (S65C) and threonine (S65T) increased the intensity of green fluorescence drastically, thereby significantly raising the detection level for GFP. For GFP S65C, the detectable number of green fluorescing tobacco (BY-2) protoplasts was raised up to 19-fold, while the fluorimetricly determined fluorescence was raised by at least 2 orders of magnitude.

Estudio de la interacci��n tritr��fica: tomate, Meloidogyne javanica y Pochonia chlamydosporia

Los nematodos fitopat��genos y en particular los agalladores causan graves p��rdidas al tomate, un cultivo de gran importancia econ��mica en Espa��a, la Uni��n Europea y en todo el mundo. El manejo de enfermedades por nematicidas qu��micos y fumigantes est�� muy limitado por la prohibici��n en la Uni��n Europea y a escala mundial del uso de muchos nematicidas qu��micos y de fumigantes como el bromuro de metilo. Los suelos supresivos a nematodos son ejemplos de control biol��gico natural que incluyen antagonistas de nematodos con potencial para su uso en el manejo de dichos pat��genos vegetales. Nuestro grupo posee una dilata experiencia en estudio de la biolog��a y en particular en el an��lisis del modo de acci��n del hongo par��sito de huevos de nematodos, Pochonia chlamydosporia. En este art��culo incluimos un resumen de nuestros estudios sobre presencia de P. chlamydosporia en suelos agr��colas. A continuaci��n abordamos el estudio de aspectos celulares y moleculares de la infecci��n de huevos de nematodos por P. chlamydosporia. Nuestro grupo fue pionero al demostrar que los hongos nemat��fagos se comportan como end��fitos colonizando las ra��ces de mono y dicotiled��neas. Recientemente hemos secuenciado el genoma de P. chlamydosporia. El estudio de sus relaciones filogen��micas y el an��lisis de sus familias g��nicas apoyan el comportamiento multitr��fico del hongo. Finalmente aportamos nuestros resultados del an��lisis metabol��mico de la interacci��n tomate-nematodo agallador-P. chlamydosporia. Nuestra actual hip��tesis de trabajo es que el estudio de dicha interacci��n por t��cnicas de an��lisis molecular masivo abren nuevas v��as al manejo sostenible de nematodos bloqueando su comunicaci��n con la planta y activando o modulando las defensas de los cultivos por hongos antagonistas como P. chlamydosporia.

Solvent-dependent enantioswitching in the Michael addition of ��,��-disubstituted aldehydes to maleimides organocatalyzed by mono-N-Boc-protected cyclohexa-1,2-diamines

Enantiomerically pure mono-N-Boc-protected trans-cyclohexa-1,2-diamines are used as organocatalysts for the enantioselective conjugate addition of ��,��-disubstituted aldehydes to maleimides. Using a single enantiomer of the organocatalyst, both enantiomeric forms of the resulting Michael adducts bearing a new quaternary stereocenter are obtained in high yields, by only changing the reaction solvent from chloroform (up to 86% ee) to aqueous DMF (up to 84% ee).

Phorbol-12-myristate-13-acetate induces nociceptin in human Mono Mac 6 cells via multiple transduction signalling pathways.

BACKGROUND Nociceptin in the peripheral circulation has been proposed to have an immunoregulatory role with regards to inflammation and pain. However, the mechanisms involved in its regulation are still not clear. The aim of this study was to investigate signalling pathways contributing to the regulation of the expression of nociceptin under inflammatory conditions. METHODS Mono Mac 6 cells (MM6) were cultured with or without phorbol-12-myristate-13-acetate (PMA). Prepronociceptin (ppNOC) mRNA was detected by RT-qPCR and extracellular nociceptin by fluorescent-enzyme immunoassay. Intracellular nociceptin and phosphorylated kinases were measured using flow cytometry. To evaluate the contribution of various signalling pathways to the regulation of ppNOC mRNA and nociceptin protein, cells were pre-treated with specific kinase inhibitors before co-culturing with PMA. RESULTS ppNOC mRNA was expressed in untreated MM6 at low concentrations. Exposure of cells to PMA upregulated ppNOC after nine h compared with controls without PMA (median normalized ratio with IQR: 0.18 (0.15-0.26) vs. 0 (0-0.02), P<0.01). Inhibition of mitogen-activated protein kinases specific for signal transduction reversed the PMA effects (all P<0.001). Induction of nociceptin protein concentrations in PMA stimulated MM6 was prevented predominantly by identity of ERK inhibitor (P<0.05). CONCLUSIONS Upregulation of nociceptin expression by PMA in MM6 cells involves several pathways. Underlying mechanisms involved in nociceptin expression may lead to new insights in the treatment of pain and inflammatory diseases.

Nueva coleccio��n de mono��logos y composiciones para recitar.

Poems and prose.

Murillo en la prisio��n, mono��logo en verso.

Mode of access: Internet.

Impresiones; mono��logo en verso

Mode of access: Internet.

Icono-mono-bibliographie des petits formats in-24 du 18e sie��cle. Collection de Lyon avec copie inverse de ses gravures a�� l'e��dition similaire en paralle��le dans la collection rivale dite de Cazin ...

A reissue of p. [19]-90 of the author's Petits joyaux bibliophiliques [1894] Also issued in combination with his Manuel du Cazinophile, the whole forming a supplement to "Cazin, sa vie et ses e��ditions; par un Cazinophile" [Brissart-Binet] Reims, 1877.

The preparation of mono- and di-substitution products of trichloro-s-triazine by replacement of chlorine by trialkylsilylphenyl

"Materials Central, Contract no. AF 33(616)-6480, Project no. 7340."

Ueber die Ortho-Derivate des Mono- und Dialkylanilins.

Thesis (doctoral)--Universitat Basel.

Geology of the Little Antelope Valley clay deposits, Mono County, California.

Mode of access: Internet.