IL-12 and neutralization of endogenous IL-10 revert the in vitro antigen-specific cellular immunosuppression of paracoccidioidomycosis patients

Treatment of patients with paracoccidioidomycosis is still a challenge. Patients present defective lymphoproliferation and IFN-γ responses to the main Paracoccidioides brasiliensis antigen (gp43), which correlates with disease severity. Here, we demonstrated that the patients show also a defective synthesis of interleukin (IL)-12. Therefore, we attempted to revert this immune disfunction by adding IL-12 and neutralizing anti-IL-10 antibody to gp-43-stimulated peripheral blood mononuclear cell cultures. Both treatments increased IFN-γ secretion to levels observed with healthy sensitized individuals, but affected proliferation only modestly. When combined, the treatments further increased IFN-γ synthesis and cell proliferation. The addition of suboptimal concentrations of IL-2 also further increased the IL-12-mediated secretion of IFN-γ. Interestingly, the immune modulation was mostly antigen-specific, since the responses to Candida albicans' antigen were not affected. These results suggest that appropriate immune intervention with cytokines and/or anti-cytokines may help in the treatment of PCM. © 2002 Elsevier Science Ltd. All rights reserved.

Diálogos intertextuais no feminino: Margaret Drabble

In The Middle Ground (1980), Margaret Drabble uses an intertextual web to depict the feminine search for identity and psychological integrity in middle age. This paper attempts to identify and analyze the absorption and integration of other texts in The Middle Ground. The dialogue, both in theme and style, with Virginia Woolf's Mrs Dalloway is initially considered, mostly from the perspective of parody. Other confluences are also studied: with Russian fairy tales, with paintings by Hans Holbein, J. B.Vanmour, Van Dyck, Claude Lorrain and Peter de Hooch, and also its references to the whole of Drabble's literary work.

Mensuração de hormônios andrógenos, estrógeno, fosfatase ácida prostática (PAP) e antígeno prostático específico (PSA) em cães adultos com próstata normal e hiperplásica

Prostatic diseases have been a common problem in middle age and older intact male dogs. Among these, benign prostatic hyperplasia (BHP) is the most frequent, age-related and hormonal-dependent condition of human and canine prostate. Blood samples were collected from 37 male intact dogs, tree years old dogs or more to determine androgens, estrogen, prostatic acid phosphatase (PAP) and prostatic specific antigen (PSA) according to histopathological aspects. Low levels of estrogen and high levels of prostatic specific antigen (PSA) were founded in dogs with BHP, respectively. Seric and urinary PAP levels were high in dogs with hyperplasia.

Long-term effects of perinatal androgenization on reproductive parameters of male rat offspring androgenization and male rat reproduction

It is known that during sex differentiation, fetal androgens are critical determinants of the male phenotype. Although testosterone is necessary for normal development of male sexual behavior, perinatal androgen treatment can result in disruption of normal male sexual reproduction. Pregnant Wistar rats were administered either corn oil (vehicle) or testosterone propionate at 0.2 mg/kg from gestational day 12 until the end of lactation and the reproductive function of male offspring was evaluated at 90 (adulthood) and 270 (middle age) days of age. Perinatal androgenization in the rat provoked a reduction in sperm production and reserves in adulthood that did not affect fertility and did not persist at more advanced ages, as shown by the results at post-natal day 270. If perinatal androgenization promotes similar effects in humans of reproductive age, the results of the present work can impact male reproduction health, given the less efficient spermatogenesis and lower sperm reserves in the human epididymis, compared to rodents. © Georg Thieme Verlag KG Stuttgart. New York.

Características comportamentais do controle postural de jovens, adultos e idosos

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

A propriedade intelectual no mundo contemporâneo no contexto geral das relações de propriedade

Pós-graduação em Ciências Sociais - FFC

A representação do caipira na UNESP FM

Pós-graduação em Comunicação - FAAC

Três histórias de Afonso Henriques, compilação, reprodução e reconstrução de uma trajetória e de uma imagem

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

O despojamento em São Bernardo de Claraval e São Francisco de Assis (séculos XII e XIII)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

O parvo em Gil Vicente e o gracioso em Antônio José da Silva: expressões do cômico no teatro português

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Da geografia medieval às origens da geografia moderna: contrastes entre diferentes noções de natureza, espaço e tempo

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

As hagiografias como instrumentos de difusão do cristianismo católico nos meios rurais da Espanha visigótica

Pós-graduação em História - FCLAS

Território, legislação e monarquia no reinado de Alfonso X, o Sábio (1252-1284)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Vogais pretônicas /e/ e /o: um estudo em tempo aparente

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

A poética interartes na tessitura de “O Ouvido” de José Saramago

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)